桧木纯露解酒护肝作用的实验研究
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摘要
为考察桧木纯露的解酒防醉效果及其对小鼠酒精性肝损伤的保护作用,将100只雄性小鼠随机分为模型组、阳性药对照组(0.15 mL/10 g)、桧木纯露高、中、低剂量组(0.2、0.15、0.1 m L/10 g)。通过灌胃各组小鼠56度红星二锅头(0.15 mL/10 g),观察其醉酒潜伏期和醒酒时间研究其防醉与解酒活性。同时各组小鼠灌胃相应药物连续14 d,测定小鼠血清中天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)及肝组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)水平,并计算肝脏脏器系数。结果表明,各给药组小鼠的醉酒潜伏期较模型组小鼠相比延长且醒酒时间缩短(P<0.05或P<0.01),桧木纯露组小鼠血清AST及ALT活性下降,肝组织中SOD水平升高MDA水平降低(P<0.05或P<0.01),肝脏系数降低(P<0.05或P<0.01)。说明桧木纯露具有防醉解酒作用,可增强酒精中毒小鼠的抗氧化能力,对小鼠酒精性肝损伤具有一定的保护作用。
In order to investigate the effect of Guimu Chunlu(GMCL)on antialcoholism and liver protection in mice,100 male mice were randomly divided into model group,positive drug control group(0.15 mL/10 g),GMCL high,medium and low dose groups(0.2,0.15,0.1 mL/10 g). The mice were administrated 0.15 mL/10 g ethanol,the latent time of drunkenness and the durative time of drunkenness were calculated in all groups. Mice were administrated the related drugs for 14 d,the activities of serum ALT(aspartate aminotransferase)and AST(alanine aminotransferase),the content of MDA(malondialdehyde)and SOD(superoxide dismutase)in liver were measured. And the liver index was calculated. The results showed that GMCL could significantly(P <0.05 or P <0.01) postpone the tolerance time of mice to the alcohol,and shorten the duration of drunkenness obviously. GMCL could markedly decrease the activities of AST and ALT,the level of MDA and liver index,increase the activities of SOD(P<0.05 or P<0.01). It showed that GMCL had beneficial effects on preventing drunkenness and relieving alcoholism. It could reduce liver injury induced by ethanol in mice.
In order to investigate the effect of Guimu Chunlu(GMCL)on antialcoholism and liver protection in mice,100 male mice were randomly divided into model group,positive drug control group(0.15 mL/10 g),GMCL high,medium and low dose groups(0.2,0.15,0.1 mL/10 g). The mice were administrated 0.15 mL/10 g ethanol,the latent time of drunkenness and the durative time of drunkenness were calculated in all groups. Mice were administrated the related drugs for 14 d,the activities of serum ALT(aspartate aminotransferase)and AST(alanine aminotransferase),the content of MDA(malondialdehyde)and SOD(superoxide dismutase)in liver were measured. And the liver index was calculated. The results showed that GMCL could significantly(P <0.05 or P <0.01) postpone the tolerance time of mice to the alcohol,and shorten the duration of drunkenness obviously. GMCL could markedly decrease the activities of AST and ALT,the level of MDA and liver index,increase the activities of SOD(P<0.05 or P<0.01). It showed that GMCL had beneficial effects on preventing drunkenness and relieving alcoholism. It could reduce liver injury induced by ethanol in mice.
引文
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[3]陈兴芬,单承莺,马世宏,等.侧柏叶化学成分、生理活性及防脱发功能研究进展[J].中国野生植物资源, 2010, 29(3):1-5
[4]赵永光,常立新,周永国,等.超声波法提取侧柏叶片中黄酮类化合物的研究[J].河北农业大学学报, 2005, 28(2):54-56
[5]刘廷礼,邱琴,赵怡,等.侧柏叶挥发油成分的GC-MS分析[J].中药材, 2000, 23(8):460-461
[6]黄立峰,朱乾坤.金线莲提取物对小鼠急性酒精性肝损伤的保护作用[J].福州总医院学报, 2005(z1):279-280
[7]袁军清,蔡明来.柴芦疏肝解毒胶囊对动物实验性肝损伤的保护作用[J].中国医药指南, 2011, 9(24):42-43
[8]李丹,郭春林,王春艳.郁芝胶囊对大鼠酒精性脂肪肝的治疗作用[J].中国老年学, 2012, 32(6):1200-1202
[9]陈兴芬,单承莺,马世宏,等.侧柏叶化学成分、生理活性及防脱发功能研究进展[J].中国野生植物资源, 2010, 29(3):1-5
[10]赵永光,常立新,周永国,等.超声波法提取侧柏叶片中黄酮类化合物的研究[J].河北农业大学学报, 2005, 28(2):54-56
[11]刘廷礼,邱琴,赵怡,等.侧柏叶挥发油成分的GC-MS分析[J].中药材, 2000, 23(8):460-461
[12]李国栋,陈园园,王盼,等.野菊花中萜类和黄酮类化合物保肝作用研究[J].中草药, 2013(24):3510-3514