参黄降糖汤对2型糖尿病大鼠胰岛素抵抗机制及瘦素水平影响研究
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摘要
目的:探讨参黄降糖汤对2型糖尿病大鼠胰岛素抵抗机制及瘦素水平影响。方法:选取清洁级SD雄性大鼠120只依据随机数字表法将其分成六组,对照组(20只)、模型组(20只)、吡格列酮组(20只)、低剂量组(20只)、中剂量组(20只)及高剂量组(20只);大鼠禁食12小时,将配制好的STZ溶液依据60mg/kg注射入大鼠腹腔内,在上述基础上使用高脂高胆固醇饲料继续喂养4周造模;低剂量、中剂量及高剂量组大鼠参黄降糖汤灌胃,剂量分别为0. 7g/kg·d、1. 4g/kg·d、2. 8g/kg·d灌胃,吡格列酮组大鼠给予吡格列酮1mg/kg·d,对照组和模型组使用10ml/d蒸馏水灌胃,每天一次,共进行八周;观察大鼠一般状况,检测六组大鼠空腹血糖(FPG)、甘油三脂(TG)及胆固醇(CHOL)含量,ELISA法检测大鼠血清瘦素及胰岛素含量,计算大鼠胰岛素抵抗指数(HOMA-IR)。结果:中、高剂量组大鼠FPG、TG含量均低于模型组及低剂量组,差异有统计学意义(P<0. 05);中、高剂量组大鼠血清瘦素含量、胰岛素及HOMA-IR低于模型组及低剂量组,差异均有统计学意义(P<0. 05);中、高剂量组大鼠血清瘦素含量、胰岛素及HOMA-IR高于对照组,差异均有统计学意义(P<0. 05)。中、高剂量组与吡格列酮组大鼠FPG、TG、大鼠血清瘦素含量、胰岛素及HOMA-IR无明显差异,差异无统计学意义(P> 0. 05)。结论:参黄降糖汤可明显改善糖尿病大鼠各项症状,调节血清瘦素、胰岛素含量。
Objective: To analyze the effect of Shenhuang Jiangtang Decoction on insulin resistance and leptin level for type2 diabetic rats. Methods: 100 clean SD male rats were divided into four groups according to the random number table method:controlled group(20rats),diabetic model group(20rats),pioglitazone group(20rats),medium dose group(20rats) and high dose group(20rats),rats fasted for 12 h,the prepared STZ solution based on 60mg/kg injected into the abdominal cavity of rats,continuous injection of 4weeks in high fat feed to continue feeding 4weeks modeling. The rats in the medium dose and the high dose groups were gavage with Shenhuang Jiangtang Decoction at the doses of 1. 4g/kg and 2. 8g/kg,respectively. The controlled group was given gavage with 30g/kg distilled water once daily for eight weeks. The general condition of the rats was observed. The levels of fasting blood glucose(FPG),triglyceride(TG) and cholesterol(CHOL) in the five groups were detected.The levels of serum leptin and insulin in the rats were measured by ELISA. The resistance index-IR was caculated. Results: The contents of FPG and TG in middle and the high dose groups were lower than those in the model group and the low dose group(P<0. 05). The levels of serum leptin,insulin and HOMA-IR in middle-dose and the high-dose groups were higher than those in the controlled group(P<0. 05),and the differences were statistically significant(P<0. 05). The levels of serum leptin,insulin and HOMA-IR of FPG,TG and rat in the middle and the high dosage groups and the pioglitazone group had no significant difference(P>0. 05). Conclusion: Shenhuang Jiangtang Decoction can significantly improve the symptoms of diabetic rats,regulate serum leptin,insulin content.
Objective: To analyze the effect of Shenhuang Jiangtang Decoction on insulin resistance and leptin level for type2 diabetic rats. Methods: 100 clean SD male rats were divided into four groups according to the random number table method:controlled group(20rats),diabetic model group(20rats),pioglitazone group(20rats),medium dose group(20rats) and high dose group(20rats),rats fasted for 12 h,the prepared STZ solution based on 60mg/kg injected into the abdominal cavity of rats,continuous injection of 4weeks in high fat feed to continue feeding 4weeks modeling. The rats in the medium dose and the high dose groups were gavage with Shenhuang Jiangtang Decoction at the doses of 1. 4g/kg and 2. 8g/kg,respectively. The controlled group was given gavage with 30g/kg distilled water once daily for eight weeks. The general condition of the rats was observed. The levels of fasting blood glucose(FPG),triglyceride(TG) and cholesterol(CHOL) in the five groups were detected.The levels of serum leptin and insulin in the rats were measured by ELISA. The resistance index-IR was caculated. Results: The contents of FPG and TG in middle and the high dose groups were lower than those in the model group and the low dose group(P<0. 05). The levels of serum leptin,insulin and HOMA-IR in middle-dose and the high-dose groups were higher than those in the controlled group(P<0. 05),and the differences were statistically significant(P<0. 05). The levels of serum leptin,insulin and HOMA-IR of FPG,TG and rat in the middle and the high dosage groups and the pioglitazone group had no significant difference(P>0. 05). Conclusion: Shenhuang Jiangtang Decoction can significantly improve the symptoms of diabetic rats,regulate serum leptin,insulin content.
引文
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[15]郭明飞,高家荣,方朝晖,等.黄地安消胶囊对改善2型糖尿病GK大鼠胰岛素抵抗的影响[J].中成药,2017,39(8):1549~1554
[16] Al S M,Mojiminiyi O A,Al A A,et al. Study of Leptin and Adiponectin as Disease Markers in Subjects with Obstructive Sleep Apnea[J]. Disease Markers,2015,14(1):203~205
[17]崔维.半夏泻心汤对糖尿病大鼠胰岛素抵抗的影响[J].中医学报,2016,31(7):1008~1011
[18] Suksomboon N,Poolsup N,Ko H D K. Effect of vitamin K supplementation on insulin sensitivity:a meta-analysis[J]. Diabetes Metabolic Syndrome Obesity Targets Therapy,2017,10(6):169~177
[19]刘瑞霞,曾建平,崔德芝,等.降糖增敏汤治疗老年2型糖尿病胰岛素抵抗临床研究[J].山东中医药大学学报,2016,7(5):439~441
[20]林戎,沈忱,黄昭穗.瘦素与妊娠糖尿病患者胰岛素抵抗及胎儿发育的相关性[J].国际内分泌代谢杂志,2017,37(6):216~219
[2]叶兰,王梅,杨祖菁.脂肪细胞因子瘦素、脂联素及趋化素与胰岛素抵抗及妊娠期糖尿病的关联研究[J].现代中西医结合杂志,2015,24(2):136~139
[3] Zhang L,Song H,Ge Y,et al. Temporal relationship between diet-induced steatosis and onset of insulin/leptin resistance in male wistar rats.[J]. Plos One,2015,10(2):8~13
[4]于健,邹迪莎,叶瑶,等.巴马小型猪T2DM模型胰岛素抵抗及瘦素、抵抗素水平的变化[J].山东医药,2015,3(5):10~12
[5]张民庆.现代临床方剂学(精)[M].北京:人民卫生出版社,2004:172~174
[6]储全根.加味桃核承气汤及其不同提取物对糖尿病大鼠心肌和主动脉病变的影响[D].广州中医药大学,2005
[7]郭毅飞,李雪锋,李敏,等.餐后有氧运动对初治肥胖2型糖尿病患者糖脂代谢、胰岛素抵抗及瘦素水平的影响[J].疑难病杂志,2016,15(7):706~709
[8] Wang Y,Feng Q,Niu X,et al. The Preventive Effect of Zuogui Wan on Offspring Rats’Impaired Glucose Tolerance Whose Mothers Had Gestational Diabetes Mellitus[J]. Evid Based Complement Alternat Med,2016,16(7):362~367
[9]蒯美玉,马志,孙鑫,等.桑酮碱改善2型糖尿病大鼠的糖脂代谢[J].中成药,2017,4(1):21~26
[10]肖子曾,曾呈茜,戴冰,等.六味地黄汤对2型糖尿病大鼠环磷酸腺苷及脂肪磷酸二酯酶3B的影响[J].中国中医药信息杂志,2015,22(8):73~76
[11]牟健,庄贵华,尚红利.加味补阳还五汤对糖尿病大鼠血糖的影响及其抗氧化活性[J].中成药,2017,39(8):1561~1566
[12]马育轩,于国强,张洪财,等.针刺配合黄连温胆汤加减对代谢综合征大鼠胰岛素抵抗及脂肪细胞因子的影响[J].中医药信息,2017,3(2):71~74
[13] Wjidan K,Ibrahim E,Caszo B,et al. Dysregulation of Glucose Homeostasis Following Chronic Exogenous Administration of Leptin in Healthy Sprague-Dawley Rats[J]. Journal of Clinical Diagnostic Research Jcdr,2015,9(12):6~9
[14]赵海英,王杏芹,牛志浩,等.脂联素、瘦素、抵抗素与妊娠糖尿病胰岛素抵抗相关性分析[J].医学动物防制,2015,10(9):1042~1044
[15]郭明飞,高家荣,方朝晖,等.黄地安消胶囊对改善2型糖尿病GK大鼠胰岛素抵抗的影响[J].中成药,2017,39(8):1549~1554
[16] Al S M,Mojiminiyi O A,Al A A,et al. Study of Leptin and Adiponectin as Disease Markers in Subjects with Obstructive Sleep Apnea[J]. Disease Markers,2015,14(1):203~205
[17]崔维.半夏泻心汤对糖尿病大鼠胰岛素抵抗的影响[J].中医学报,2016,31(7):1008~1011
[18] Suksomboon N,Poolsup N,Ko H D K. Effect of vitamin K supplementation on insulin sensitivity:a meta-analysis[J]. Diabetes Metabolic Syndrome Obesity Targets Therapy,2017,10(6):169~177
[19]刘瑞霞,曾建平,崔德芝,等.降糖增敏汤治疗老年2型糖尿病胰岛素抵抗临床研究[J].山东中医药大学学报,2016,7(5):439~441
[20]林戎,沈忱,黄昭穗.瘦素与妊娠糖尿病患者胰岛素抵抗及胎儿发育的相关性[J].国际内分泌代谢杂志,2017,37(6):216~219