荧光定量PCR法检测乙肝病毒DNA和ELISA法检测乙肝病毒标志物的临床价值
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摘要
目的探究荧光定量PCR法检测乙肝病毒DNA和ELISA法检测乙肝病毒标志物的临床价值。方法本研究选取于2016年1月～2018年2月在本院就诊的乙肝患者共4355例,采用ELISA法,对患者进行定性检测,采用荧光定量PCR法,对患者的病毒水平进行检测。结果 4355例乙肝患者,1(+)、3(+)、5(+)模式的阳性率最高,与其他组间比较差异有统计学意义(P <0.05)。4355例乙肝患者,1(+)、4(+)、5(+)模式的患者数量最多,2(+)、4(+)模式患者数量最少,与其他组比较差异有统计学意义(P <0.05)。结论在不同的HBV-DNA定量测定模式下,所测量出来的阳性率及HBV-DNA水平存在着一定的差异,为了提升乙肝病毒的临床诊断准确率,在乙肝病毒DNA(HBV-DNA)和乙肝病毒标志物(HBV-M)中的临床诊断中,应采用荧光定量PCR法及ELISA法,为乙肝病毒的高效治疗提供依据。
Objective To explore the clinical value of fluorescence quantitative PCR(FQ-PCR) in the detection of hepatitis B virus DNA(HBV-DNA) and ELISA in the detection of hepatitis B virus marker(HBV-M). Methods 4 355 patients with hepatitis B in our hospital from January 2016 to February 2018 were selected,the patients were qualitative tested by ELISA,the patient's virus level were detected by fluorescence quantitative PCR. Results Among 4355 hepatitis B patients, the positive rates of 1(+),3(+),and 5(+) patterns were the highest,and the difference was statistically significant(P < 0.05).Among the 4355 patients with hepatitis B,the number of patients in the 1(+),4(+),and 5(+) patterns were the highest,and the number of patients in the 2(+) and 4(+) patterns were the least.The difference was statistically significant(P < 0.05). Conclusion There are some differences in the positive rate and HBV-DNA levels measured in different HBV-DNA quantitative assays.In order to improve the clinical diagnostic accuracy of hepatitis B virus,in the clinical diagnosis of hepatitis B virus DNA(HBV-DNA) and hepatitis B virus markers(HBV-M),fluorescent quantitative PCR and ELISA should be used to improve the efficiency of hepatitis B virus.Provide a basis for treatment.
Objective To explore the clinical value of fluorescence quantitative PCR(FQ-PCR) in the detection of hepatitis B virus DNA(HBV-DNA) and ELISA in the detection of hepatitis B virus marker(HBV-M). Methods 4 355 patients with hepatitis B in our hospital from January 2016 to February 2018 were selected,the patients were qualitative tested by ELISA,the patient's virus level were detected by fluorescence quantitative PCR. Results Among 4355 hepatitis B patients, the positive rates of 1(+),3(+),and 5(+) patterns were the highest,and the difference was statistically significant(P < 0.05).Among the 4355 patients with hepatitis B,the number of patients in the 1(+),4(+),and 5(+) patterns were the highest,and the number of patients in the 2(+) and 4(+) patterns were the least.The difference was statistically significant(P < 0.05). Conclusion There are some differences in the positive rate and HBV-DNA levels measured in different HBV-DNA quantitative assays.In order to improve the clinical diagnostic accuracy of hepatitis B virus,in the clinical diagnosis of hepatitis B virus DNA(HBV-DNA) and hepatitis B virus markers(HBV-M),fluorescent quantitative PCR and ELISA should be used to improve the efficiency of hepatitis B virus.Provide a basis for treatment.
引文
[1]高娟,刘静.荧光定量PCR法与ELISA法联合检测乙型肝炎的临床价值探讨[J].中国优生与遗传杂志,2017,25(9):29-31.
[2]Yao Zhaoji.Real-time fluorescence quantitative PCR for the detection of hepatitis B virus DNA and hepatitis B virus markers.[J].Inner Mongolia Journal of medicine,2017,49(4):485-486.
[3]DUKAI.Real-time fluorescence quantitative PCR method for the detection and analysis of the value of hepatitis Bvirus DNA[J].Chinese modern medicine application,2017,11(1):55-57.
[4]梁静仪,李海燕,姚春松.荧光定量PCR法与ELISA法检测乙肝病毒感染相关性探讨[J].临床医学工程,2015,22(8):1044-1045.
[5]陈海雁,张桂花,罗锦彬,等.荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析[J].实用临床医药杂志,2014,18(19):108-110.
[6]Chen Haiyan,Zhang Guihua,Luo Jinbin,et al.The analysis of the clinical value of detection of hepatitis B virus DNA and HBV markers by fluorescence quantitative PCRmethod[J].International Journal of laboratory medicine,2014,35(16):2216-2217.
[7]Wang Haifeng.PCR and ELISA for detection of hepatitis Bvirus[J].Journal of Heze Medical College,2014,26(2):12-13,48.
[8]Zhou Yulin,Zheng Jinchuan,Wu Xuyao.Detection of the correlation between hepatitis B virus DNA and HBVmarkers by fluorescence quantitative PCR method[J].Chinese rural health management,2013,33(10):1191-1192.
[9]王欣,朱海燕,王宇.乙肝患者检测血浆D-D二聚体及HBV-DNA的临床意义[J].系统医学,2016,1(10):26-28.
[10]陈海雁,张桂花,罗锦彬,等.荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析[J].国际检验医学杂志,2014,35(16):2216-2217.
[11]汪圣强,耿合员,殷鹏飞,等.实时荧光PCR在乙肝病毒DNA检测中的临床意义[J].中国医学工程,2015,23(3):29-30.
[12]颜敏.血清HBV-DNA荧光定量PCR检测与乙肝病毒标志物模式的相关因素探讨[J].中外医疗,2014,23(26):193-194.
[13]吴洪秋,张永良,黄坚尧,等.乙肝血清学标志物定量和HBVDNA定量联合检测在乙肝病毒感染诊断中的应用[J].胃肠病学和肝病学杂志,2016,25(4):415-418.
[14]成克铭,闵瑶.2007例乙肝标志物?HBV-DNA及ALT检测结果分析及临床价值[J].国际检验医学杂志,2016,37(7):987-989.
[2]Yao Zhaoji.Real-time fluorescence quantitative PCR for the detection of hepatitis B virus DNA and hepatitis B virus markers.[J].Inner Mongolia Journal of medicine,2017,49(4):485-486.
[3]DUKAI.Real-time fluorescence quantitative PCR method for the detection and analysis of the value of hepatitis Bvirus DNA[J].Chinese modern medicine application,2017,11(1):55-57.
[4]梁静仪,李海燕,姚春松.荧光定量PCR法与ELISA法检测乙肝病毒感染相关性探讨[J].临床医学工程,2015,22(8):1044-1045.
[5]陈海雁,张桂花,罗锦彬,等.荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析[J].实用临床医药杂志,2014,18(19):108-110.
[6]Chen Haiyan,Zhang Guihua,Luo Jinbin,et al.The analysis of the clinical value of detection of hepatitis B virus DNA and HBV markers by fluorescence quantitative PCRmethod[J].International Journal of laboratory medicine,2014,35(16):2216-2217.
[7]Wang Haifeng.PCR and ELISA for detection of hepatitis Bvirus[J].Journal of Heze Medical College,2014,26(2):12-13,48.
[8]Zhou Yulin,Zheng Jinchuan,Wu Xuyao.Detection of the correlation between hepatitis B virus DNA and HBVmarkers by fluorescence quantitative PCR method[J].Chinese rural health management,2013,33(10):1191-1192.
[9]王欣,朱海燕,王宇.乙肝患者检测血浆D-D二聚体及HBV-DNA的临床意义[J].系统医学,2016,1(10):26-28.
[10]陈海雁,张桂花,罗锦彬,等.荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析[J].国际检验医学杂志,2014,35(16):2216-2217.
[11]汪圣强,耿合员,殷鹏飞,等.实时荧光PCR在乙肝病毒DNA检测中的临床意义[J].中国医学工程,2015,23(3):29-30.
[12]颜敏.血清HBV-DNA荧光定量PCR检测与乙肝病毒标志物模式的相关因素探讨[J].中外医疗,2014,23(26):193-194.
[13]吴洪秋,张永良,黄坚尧,等.乙肝血清学标志物定量和HBVDNA定量联合检测在乙肝病毒感染诊断中的应用[J].胃肠病学和肝病学杂志,2016,25(4):415-418.
[14]成克铭,闵瑶.2007例乙肝标志物?HBV-DNA及ALT检测结果分析及临床价值[J].国际检验医学杂志,2016,37(7):987-989.