高效液相色谱法测定果汁中6-苄基腺嘌呤残留量
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摘要
为了建立高效液相色谱法测定果汁中6-苄基腺嘌呤残留量的检测方法,样品经甲醇超声提取, C_(18)固相萃取柱净化,浓缩定容后直接进样分析。结果表明:该方法在0.10～0.60μg·mL~(-1)范围内线性关系良好(R~2=0.999 6),加标回收率为99.00%～100.67%(加标浓度为1.0, 0.50和0.20μg·mL~(-1)), RSD值为0.31%～0.79%(n=6),方法检出限为7.58×10~(-3)μg·kg~(-1),定量限为2.53×10~(-2)μg·kg~(-1),重复性(n=6)为1.1%,整个提取分离测定流程快速、简便、准确、毒性小,适用于果汁饮料中6-苄基腺嘌呤残留量的测定。
To develope a multi-residue determination method for 6-benzylaminopurine in juice by high performance liquid chromatography(HPLC), the samples were extracted by methanol with ultrasonic and purified by with C_(18) solid phase extraction column. After being concentrated and quantified, the samples were directly injected. The results showed a good linear relationship of 6-benzylaminopurine with the content from 0.10 to 0.60 μg·mL~(-1). The correlation coeffeicient R~2 was 0.999 6, the recoveries of standard addition was within 99.00%-100.67%(standard additive concentrations were 1.0, 0.50 and 0.20 μg·mL~(-1)), the RSD was within 0.31%-0.79%(n=6), the limit of detection was 7.58×10~(-3) μg·kg~(-1), the quantitation was 2.53× 10~(-2) μg·kg~(-1), and the repeatability was 1.1%(n=6). It was proved that this procedure is rapid, simple, accurate and no toxicity for efficient determination of 6-benzylaminopurine in juice.
To develope a multi-residue determination method for 6-benzylaminopurine in juice by high performance liquid chromatography(HPLC), the samples were extracted by methanol with ultrasonic and purified by with C_(18) solid phase extraction column. After being concentrated and quantified, the samples were directly injected. The results showed a good linear relationship of 6-benzylaminopurine with the content from 0.10 to 0.60 μg·mL~(-1). The correlation coeffeicient R~2 was 0.999 6, the recoveries of standard addition was within 99.00%-100.67%(standard additive concentrations were 1.0, 0.50 and 0.20 μg·mL~(-1)), the RSD was within 0.31%-0.79%(n=6), the limit of detection was 7.58×10~(-3) μg·kg~(-1), the quantitation was 2.53× 10~(-2) μg·kg~(-1), and the repeatability was 1.1%(n=6). It was proved that this procedure is rapid, simple, accurate and no toxicity for efficient determination of 6-benzylaminopurine in juice.
引文
[1]董丽红.6-苄基腺嘌呤--植物生长调节剂[J].化学教学,2011(3):66-67.
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[5]杨途煕,魏安智,郑元,等.高效液相色谱法同时测定杏仁中8种植物激素[J].分析化学,2007(9):1359-1361.
[6]王成梅,张广昊.液相色谱-质谱联用仪测定蔬菜中6-苄基腺嘌呤的研究[J].食品安全导刊,2016(9):139-140.
[7]谢君,张义正.植物内源激素的反相高效液相色潽法测定[J].分析测试学报,2001,20(1):60-62.
[8]赵数兰,韩藴华,乔艳红,等.豆科植物促长剂中6-苄基腺嘌呤的RPLC研究[J].天津师大学报(自然科学版),1998,18(1):43-46.
[2]SETYADJIT,JOYCE D C,IRVING D E,et al.Effect of6-benzylaminopurine treatment on the longevity of harvested grevillea‘Sylvia’inflorescences[J].Plant Growth Regulation,2004(43):9-14.
[3]FENG X,YANG Z F,CHEN X H,et al.6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli[J].Journal of Agricultural&Food Chemistry,2012,60(1):234-240.
[4]金米聪,陈晓红,李小平,等.HPLC/APCI/MS法测定水果中6-苄基腺嘌呤[J].分析仪器,2005(3):29-31.
[5]杨途煕,魏安智,郑元,等.高效液相色谱法同时测定杏仁中8种植物激素[J].分析化学,2007(9):1359-1361.
[6]王成梅,张广昊.液相色谱-质谱联用仪测定蔬菜中6-苄基腺嘌呤的研究[J].食品安全导刊,2016(9):139-140.
[7]谢君,张义正.植物内源激素的反相高效液相色潽法测定[J].分析测试学报,2001,20(1):60-62.
[8]赵数兰,韩藴华,乔艳红,等.豆科植物促长剂中6-苄基腺嘌呤的RPLC研究[J].天津师大学报(自然科学版),1998,18(1):43-46.