粉色、黑色花生种皮色素的抗氧化活性及组分差异分析
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摘要
为了深入了解粉色和黑色花生种皮色素的抗氧化活性及组分差异,采用高效液相色谱-高分辨飞行时间质谱(UPLC-Q-TOF-MS)联用仪分析了粉色和黑色花生种皮色素的组分,从粉色花生种皮色素中鉴定出(表)儿茶素和2种原花色素二聚体、1种原花色素三聚体和2种原花色素四聚,黑色花生种皮色素中鉴定出原花色素二聚体、三聚体、四聚体和2种矢车菊素、4种飞燕草素类组分。采用分光光度法测定了粉色、黑色花生种皮色素的抗氧化活性,粉色花生种皮色素的抗氧化能力为(17.46±1.88) U/mg,黑色花生种皮色素的总抗氧化能力(23.77±1.58) U/mg;黑色花生种皮色素对羟自由基和超氧阴离子的半抑制力IC_(50)分别为0.58、1.79 mg/m L;粉色花生种皮色素对羟自由基和超氧阴离子的半抑制力IC_(50)分别为0.39、1.41 mg/m L。2种不同颜色花生种皮色素的组分具有明显差异,其抗氧化活性也存在异同之处,黑色花生总抗氧化能力强于粉色花生种皮色素,而抑制羟自由基和超氧阴离子的能力弱于粉色花生种皮色素。
To have a deep understanding of the difference in antioxidant activity and composition of the seed coat pigments between pink and black peanuts,the composition of seed coat pigments from pink and black peanuts were analyzed by UPLCQ-TOF-MS.Catechin,two dimer,one trimer and two tetramers of proanthocyanidins were identified in the pink peanut coat,while two cyanidin,four delpinidin besides dimer,trimer and tetramers of proanthocyanidins were identified in black peanuts seed coat.The antioxidant activity of pink and black peanut seed coat pigment were determined by spectrophotometry.The results showed that the antioxidant activity of pink peanut seed coat pigments was( 17.46 + 1.88) U/mg.the antioxidant activity of black peanut seed coat pigments was( 23.77 + 1.58) U/mg; the semi-inhibitory effect IC_(50) of black peanut seed coat pigments on hydroxyl radicals and superoxide anions were 0.58 and 1.79,respectively,and that of red peanut seed coat pigments on hydroxyl Semi-inhibitory IC_(50) of oxygen anion were 0.39 and 1.41 mg/m L. Its compounds and antioxidant activity had obvious differences.The total antioxidant activity of black peanut was stronger than that of pink peanut,while the ability of inhibiting hydroxyl radical and superoxide anion was weaker than that of pink peanut.
To have a deep understanding of the difference in antioxidant activity and composition of the seed coat pigments between pink and black peanuts,the composition of seed coat pigments from pink and black peanuts were analyzed by UPLCQ-TOF-MS.Catechin,two dimer,one trimer and two tetramers of proanthocyanidins were identified in the pink peanut coat,while two cyanidin,four delpinidin besides dimer,trimer and tetramers of proanthocyanidins were identified in black peanuts seed coat.The antioxidant activity of pink and black peanut seed coat pigment were determined by spectrophotometry.The results showed that the antioxidant activity of pink peanut seed coat pigments was( 17.46 + 1.88) U/mg.the antioxidant activity of black peanut seed coat pigments was( 23.77 + 1.58) U/mg; the semi-inhibitory effect IC_(50) of black peanut seed coat pigments on hydroxyl radicals and superoxide anions were 0.58 and 1.79,respectively,and that of red peanut seed coat pigments on hydroxyl Semi-inhibitory IC_(50) of oxygen anion were 0.39 and 1.41 mg/m L. Its compounds and antioxidant activity had obvious differences.The total antioxidant activity of black peanut was stronger than that of pink peanut,while the ability of inhibiting hydroxyl radical and superoxide anion was weaker than that of pink peanut.
引文
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[24]邝敏杰,齐敏玉,何静仁,等.紫菜薹花色苷组分鉴定及其稳定性和抗氧化性[J].中国农业科学,2014,47(20):4067-4077.
[25]爦tef2nu爫M N,C2ta A,Pop R,et al.Anthocyanins HPLC-DAD and MS characterization,total phenolics,and antioxidant activity of some berries extracts[J].Analytical Letters,2011,44(18):2843-2855.
[26]张智猛,万书波,戴良香,等.不同类型花生品种籽仁部位抗氧化能力及功能成分研究[J].食品与生物技术学报,2009,28(6):741-748.
[27]肖春玲,田理刚,岳思远,等.不同色泽花生衣抗氧化活性研究[J].食品研究与开发,2017,38(7):9-13.
[2]霍婷,杨慧玲,薛文通,等.花生红衣色素的研究进展[J].食品工业科技,2008,29(11):289-295.
[3]杨庆利,张吉民.花生功能成分加工学[M].北京:中国农业出版社,2010:352.
[4]Phan-Thien K Y,Wright G C,Lee N A.Peanut antioxidants:Part 2.Quantitation of free and matrix-bound phytochemicals in five selected genotypes with diverse antioxidant capacity by high performance liquid chromatography(HPLC)[J].LWT-Food Science and Technology,2014,57(1):312-319.
[5]Bi J,Yang Q L,Yu L N,at al.Evaluation of antioxidant activity of alcoholic extract from peanut hulls[J].Advanced Materials Research,2011,233-235:2849-2854.
[6]刘连红,陈飞,张丽,等.花生的药用成分及其提取分离技术的研究进展[J].生物加工过程,2018,16(4):40-48.
[7]许胜,屈达才,黄慧明,等.花色素及其花色苷单体抗肿瘤活性研究进展[J].食品工业科技,2016(14):379-384.
[8]李侠,林湖庭.姜黄素抗突变抗癌作用研究进展[J].华西药学杂志,1996,3(5):78-81.
[9]赵萍,竹军,刘冰,等.花生红衣乙醇提取物乙酸乙酯组分抑菌活性的研究[J].食品工业科技,2016,37(2):134-137.
[10]张红梅,王静,金征宇.天然红色素在肉制品中的应用[J].中国食品工业,2004(3):50-51.
[11]Munekata P E S,Calomeni A V,Rodrigues C E C,et al.Peanut skin extract reduces lipid oxidation in cooked chicken patties[J].Poultry Science,2015,94(3):442-446.
[12]韩升廷.花生红衣中多酚的提取及体外抗氧化研究[M].长春:长春工业大学,2013.
[13]杜蕾,李新华,陈芳芳.黑花生衣色素抗氧化作用的研究[J].食品工业科技,2012,33(13):100-103.
[14]Tsujita T,Shintani T,Sato H.Preparation and characterisation of peanut seed skin polyphenols[J].Food Chemistry,2014,151:15-20.
[15]杜蕾,李新.黑、红花生衣中原花色素的分析[J].食品科学,2014,35(4):190-194.
[16]Du L,Li X H,Liu Y.Determination of functional components of peanut skins pigment[J].Advanced Materials Research,2013,807:2035-2042.
[17]林茂,赵景芳,郑秀艳,等.不同颜色花生种皮色素提取工艺研究[J].花生学报,2018,47(3):32-39.
[18]欧阳新平,陈子龙,邱学青.超高效液相色谱/高分辨质谱法测定木质素氧化降解产物中单酚类化合物[J].分析化学,2014,42(5):723-728.
[19]刘国强,董静,王弘,等.4种儿茶素类化合物电离喷雾质谱裂解规律的研究[J].高等学校化学学报,2009,30(8):1566-1570.
[20]邓菊,褚仕超,刘张武,等.蚕豆皮中原花青素结构的初步鉴定[J].中国酿造,2013,32(2):137-143.
[21]徐歆,姚其凤,祝琳,等.紫娟茶原花青素的组分及活性评价[J].食品工业科技,2018,39(21):235-240.
[22]Lee J H,Kang N S,Shin S O,et al.Characterisation of anthocyanins in the black soybean(Glycine max L.)by HPLC-DAD-ESI/MS analysis[J].Food Chemistry,2009,112(1):226-231.
[23]Hao J,Zhu H,Zhang Z,et al.Identification of anthocyanins in black rice(Oryza sativa L.)by UPLC/Q-TOF-MS and their in vitro and in vivo antioxidant activities[J].Journal of Cereal Science,2015,64:92-99.
[24]邝敏杰,齐敏玉,何静仁,等.紫菜薹花色苷组分鉴定及其稳定性和抗氧化性[J].中国农业科学,2014,47(20):4067-4077.
[25]爦tef2nu爫M N,C2ta A,Pop R,et al.Anthocyanins HPLC-DAD and MS characterization,total phenolics,and antioxidant activity of some berries extracts[J].Analytical Letters,2011,44(18):2843-2855.
[26]张智猛,万书波,戴良香,等.不同类型花生品种籽仁部位抗氧化能力及功能成分研究[J].食品与生物技术学报,2009,28(6):741-748.
[27]肖春玲,田理刚,岳思远,等.不同色泽花生衣抗氧化活性研究[J].食品研究与开发,2017,38(7):9-13.