食品中黄曲霉毒素检测技术的研究进展
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摘要
黄曲霉毒素主要是由黄曲霉、寄生曲霉产生的次级代谢产物,在自然界中尤其是湿热地区分布广泛,污染范围广,具有强致癌和致突变性。目前,用于检测黄曲霉毒素含量的技术主要包括大型仪器分析技术和免疫分析技术。对当前黄曲霉毒素检测技术的研究进展进行简要综述,旨在为食品中黄曲霉毒素的检测分析提供参考。
Aflatoxins are mainly secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. It is widely distributed in nature,especially in hot and humid areas,with a wide range of pollution,and is highly carcinogenic and mutagenic. At present, the detection technology for aflatoxins mainly includes large-scale instrumental analysis technology and immunoassay technology. In this paper,a brief review of the current research progress in the detection of aflatoxins was provided to provide reference for the detection and analysis of aflatoxins in food.
Aflatoxins are mainly secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. It is widely distributed in nature,especially in hot and humid areas,with a wide range of pollution,and is highly carcinogenic and mutagenic. At present, the detection technology for aflatoxins mainly includes large-scale instrumental analysis technology and immunoassay technology. In this paper,a brief review of the current research progress in the detection of aflatoxins was provided to provide reference for the detection and analysis of aflatoxins in food.
引文
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[11]Vaclavik L,Vaclavikova M,Begley T H,et al.Determination of multiple mycotoxins in dietary supplements containing green coffee bean extracts using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry(UHPLC-MS/MS)[J].Journal of Agricultural and Food Chemistry,2013,61(20):4 822-4 830.
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[15]Li P,Zhang Q,Zhang W,et al.Development of a classspecific monoclonal antibody-based ELISA for aflatoxins in peanut[J].Food Chemistry,2009(1):313-317.
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[17]宋青龙,李成洪,傅巍,等.黄曲霉毒素B1胶体金快速定量检测试剂盒的研发[J].动物营养学报,2017(10):3 703-3 709.
[18]Zhang Z,Li Y,Li P,et al.Monoclonal antibody-quantum dots Cd Te conjugate-based fluoroimmunoassay for the determination of aflatoxin B1in peanuts[J].Food Chemistry,2014(1):314-319.
[19]Beloglazova N V,Eremin S A.Rapid screening of aflatoxin B1in beer by fluorescence polarization immunoassay[J].Talanta,2015(15):170-175.
[20]Hui L,Daibin Y,Peiwu L,et al.Palladium nanoparticlesbased fluorescence resonance energy transfer aptasensor for highly sensitive detection of aflatoxin M1in milk[J].Toxins,2017(10):318-324.
[21]Tang X,Zhang Z,Li P,et al.Sample-pretreatment-free based high sensitive determination of aflatoxin M1in raw milk using a time-resolved fluorescent competitive immunochromatographic assay[J].Rsc Advances,2014,5(1):558-564.
[22]Kanungo L,Bhand S.Fluorimetric immunoassay for multianalysis of aflatoxins[J].Journal of Analytical Methods in Chemistry,2013(7):1-8.
[23]裘雪梅,朱立鑫,刘仁荣,等.黄曲霉毒素B1化学发光免疫检测方法的建立[J].畜牧与饲料科学,2015(10):29-32.
[24]王岩,于源华,王维,等.黄曲霉毒素M1化学发光酶免疫检测方法的建立[J].食品科技,2013(5):342-346.
[25]汪玉,徐桂芳,张清榕,等.基于磁性纳米纤维/碳纳米角修饰的黄曲霉毒素电化学发光免疫传感器的研制与应用[J].广东化工,2015(9):153-160.
[2]Reddy K R N,Reddy C S,Muralidharan K.Detection of Aspergillus spp.and aflatoxin B1in rice in India[J].Food Microbiology,2009,26(1):27-31.
[3]Delmulle B S,De Saeger S M,Sibanda L,et al.Development of an immunoassay-based lateral flow dipstickfor the rapid detection of aflatoxin B1in pig feed[J].Journal of Agricultural and Food Chemistry,2005,53(9):3 364-3 368.
[4]Sweeney M J,Dobson A D.Mycotoxin production by Aspergillus,Fusarium and Penicillium species.[J].International Journal of Food Microbiology,1998,43(3):141-158.
[5]Zijden A S M V D,Koelensmid W A A B,Boldingh J.Aspergillus flavus and turkey X disease:Isolation in crys-talline form of a toxin responsible for turkey X disease[J].Nature,1962(11):1 060-1 062.
[6]Zheng H,Zheng Y,Luan L,et al.An ultra-high-performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of aflatoxins B1,B2,G1,G2,M1and M2in traditional Chinese medicines[J].Analytica Chimica Acta,2010(2):165-171.
[7]何婷.黄曲霉毒素纳米抗体研制及其免疫分析技术研究[D].北京:中国农业科学院,2016.
[8]Campos W E O,Rosas L B,Neto A P,et al.Extended validation of a senstive and robust method for simultaneous quantification of aflatoxins B1,B2,G1and G2in Brazil nuts by HPLC-FLD[J].Journal of Food Composition and Analysis,2017(6):90-96.
[9]Fu Z,Huang X,Min S.Rapid determination of aflatoxins in corn and peanuts[J].Journal of Chromatography A,2008(1-2):271-274.
[10]Lattanzio V M T,Ciasca B,Powers S,et al.Improved method for the simultaneous determination of aflatoxins,ochratoxin A and Fusarium toxins in cereals and derived products by liquid chromatography-tandem mass spectrometry after multi-toxin immunoaffinity clean up[J].Journal of Chromatography A,2014(4):139-143.
[11]Vaclavik L,Vaclavikova M,Begley T H,et al.Determination of multiple mycotoxins in dietary supplements containing green coffee bean extracts using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry(UHPLC-MS/MS)[J].Journal of Agricultural and Food Chemistry,2013,61(20):4 822-4 830.
[12]Porstmann T,Kiessig S T.Enzyme immunoassay techniques.An overview[J].Journal of Immunological Methods,1992(1-2):5-21.
[13]Lawellin D W,Grant D W,Joyce B K.Enzyme-linked immunosorbent analysis for aflatoxin B1[J].Applied&Environmental Microbiology,1977,34(1):94-96.
[14]Zhang Q.An ultra-sensitive monoclonal antibody-based competitive enzyme immunoassay for aflatoxin M1in milk and infant milk products[J].Food Chemistry,2011(4):1 359-1 364.
[15]Li P,Zhang Q,Zhang W,et al.Development of a classspecific monoclonal antibody-based ELISA for aflatoxins in peanut[J].Food Chemistry,2009(1):313-317.
[16]Fei Ma,Ran C,Peiwu L,et al.Preparation of an immunoaffinity column with amino-silica gel microparticles and its application in sample cleanup for aflatoxin detection in agri-products[J].Molecules,2013,18(2):2 222-2 235.
[17]宋青龙,李成洪,傅巍,等.黄曲霉毒素B1胶体金快速定量检测试剂盒的研发[J].动物营养学报,2017(10):3 703-3 709.
[18]Zhang Z,Li Y,Li P,et al.Monoclonal antibody-quantum dots Cd Te conjugate-based fluoroimmunoassay for the determination of aflatoxin B1in peanuts[J].Food Chemistry,2014(1):314-319.
[19]Beloglazova N V,Eremin S A.Rapid screening of aflatoxin B1in beer by fluorescence polarization immunoassay[J].Talanta,2015(15):170-175.
[20]Hui L,Daibin Y,Peiwu L,et al.Palladium nanoparticlesbased fluorescence resonance energy transfer aptasensor for highly sensitive detection of aflatoxin M1in milk[J].Toxins,2017(10):318-324.
[21]Tang X,Zhang Z,Li P,et al.Sample-pretreatment-free based high sensitive determination of aflatoxin M1in raw milk using a time-resolved fluorescent competitive immunochromatographic assay[J].Rsc Advances,2014,5(1):558-564.
[22]Kanungo L,Bhand S.Fluorimetric immunoassay for multianalysis of aflatoxins[J].Journal of Analytical Methods in Chemistry,2013(7):1-8.
[23]裘雪梅,朱立鑫,刘仁荣,等.黄曲霉毒素B1化学发光免疫检测方法的建立[J].畜牧与饲料科学,2015(10):29-32.
[24]王岩,于源华,王维,等.黄曲霉毒素M1化学发光酶免疫检测方法的建立[J].食品科技,2013(5):342-346.
[25]汪玉,徐桂芳,张清榕,等.基于磁性纳米纤维/碳纳米角修饰的黄曲霉毒素电化学发光免疫传感器的研制与应用[J].广东化工,2015(9):153-160.