儿童呼吸道感染者肺炎支原体及肺炎衣原体特异性抗体检测临床意义
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摘要
目的探究儿童呼吸道感染者肺炎支原体及肺炎衣原体特异性抗体检测临床意义。方法选择我院2018年1月～8月收治的85例呼吸道感染患儿设为研究组,另选择同期在我院进行健康体检的85例儿童设为对照组,对照组采用ELISA法检测肺炎支原体及衣原体,研究组在此基础上行咽拭子培养法检测,比较两组肺炎支原体、衣原体抗体检出率,ELISA法与咽拭子培养法对肺炎支原体、衣原体抗体的阳性检出率,分析研究组呼吸道感染性疾病中肺炎支原体、衣原体抗体阳性分布情况。结果研究组患儿肺炎支原体抗体MP-IgM抗体、MP-IgA抗体、MP-IgG抗体及肺炎衣原体特异性抗体CP-IgM抗体、CP-IgG抗体的检出率均高于对照组儿童,差异有统计学意义(P<0.05);ELISA法对肺炎支原体特异性抗体阳性的检出率为54.12%高于咽拭子培养法的36.47%,组间对比具有统计学意义(P<0.05);ELISA法对肺炎衣原体特异性抗体阳性的检出率为58.82%高于咽拭子培养法的37.65%,差异有统计学意义(P<0.05);肺炎支原体、肺炎衣原体抗体阳性在各种儿童呼吸道感染性疾病中均有表现,其中,肺炎和支气管肺炎的阳性检出率较咽炎、扁桃体炎均较高。结论肺炎支原体、肺炎衣原体是引发肺炎和支气管肺炎感染的主要病原菌。
Objective To investigate the clinical significance of detection of specific antibodies against mycoplasma pneumoniae and chlamydia pneumoniae in children with respiratory infection.Methods 85 children with respiratory infection admitted to our hospital from January to August 2018 were selected as study group,while 85 children who underwent physical examination in our hospital were selected as control group.In the control group,mycoplasma pneumoniae and chlamydia pneumoniae were detected by ELISA method,and on this basis the throat swab culture method was used in the study group.The detection rates of mycoplasma pneumoniae and chlamydia pneumoniae antibodies were compared between the two groups.The positive rates of mycoplasma pneumoniae and chlamydia antibodies were detected by ELISA and throat swab and the positive distribution of mycoplasma pneumoniae and chlamydia pneumoniae antibody in respiratory infectious diseases were analyzed in study group.Results The detection rate of mycoplasma pneumoniae antibody MP-IgM antibody, MP-IgA antibody, MP-IgG antibody,chlamydia pneumoniae specific antibody CP-IgM antibody and CP-IgG antibody in the study group were higher than those in the control group,the difference was statistically significant( P<0.05).The positive rate of specific antibody against mycoplasma pneumoniae by ELISA method was 54.12%, which was higher than that by throat swab culture method 36.47%.The comparison between groups was statistically significant(P <0.05).The positive rate of specific antibody against chlamydia pneumoniae by ELISA method was 58.82%, which was higher than that by throat swab culture method 37.65%,the difference was statistically significant( P<0.05).Mycoplasma pneumoniae, chlamydia pneumoniae antibody positive in all kinds of children respiratory infectious diseases,the positive rate of pneumonia and bronchopneumonia was higher than that of pharyngitis and tonsillitis.Conclusion Mycoplasma pneumoniae and chlamydia pneumoniae are the main pathogens causing pneumonia and bronchopneumonia.
Objective To investigate the clinical significance of detection of specific antibodies against mycoplasma pneumoniae and chlamydia pneumoniae in children with respiratory infection.Methods 85 children with respiratory infection admitted to our hospital from January to August 2018 were selected as study group,while 85 children who underwent physical examination in our hospital were selected as control group.In the control group,mycoplasma pneumoniae and chlamydia pneumoniae were detected by ELISA method,and on this basis the throat swab culture method was used in the study group.The detection rates of mycoplasma pneumoniae and chlamydia pneumoniae antibodies were compared between the two groups.The positive rates of mycoplasma pneumoniae and chlamydia antibodies were detected by ELISA and throat swab and the positive distribution of mycoplasma pneumoniae and chlamydia pneumoniae antibody in respiratory infectious diseases were analyzed in study group.Results The detection rate of mycoplasma pneumoniae antibody MP-IgM antibody, MP-IgA antibody, MP-IgG antibody,chlamydia pneumoniae specific antibody CP-IgM antibody and CP-IgG antibody in the study group were higher than those in the control group,the difference was statistically significant( P<0.05).The positive rate of specific antibody against mycoplasma pneumoniae by ELISA method was 54.12%, which was higher than that by throat swab culture method 36.47%.The comparison between groups was statistically significant(P <0.05).The positive rate of specific antibody against chlamydia pneumoniae by ELISA method was 58.82%, which was higher than that by throat swab culture method 37.65%,the difference was statistically significant( P<0.05).Mycoplasma pneumoniae, chlamydia pneumoniae antibody positive in all kinds of children respiratory infectious diseases,the positive rate of pneumonia and bronchopneumonia was higher than that of pharyngitis and tonsillitis.Conclusion Mycoplasma pneumoniae and chlamydia pneumoniae are the main pathogens causing pneumonia and bronchopneumonia.
引文
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[16]刘大宁,张丽媛.荧光定量聚合酶链反应技术在儿童肺炎支原体及肺炎衣原体感染中的应用[J].实用医技杂志,2017,24(3):288-289.
[17]陈建辉.儿童呼吸道感染者肺炎支原体、肺炎衣原体特异性抗体检测临床意义探讨[J].中国卫生检验杂志,2008(7):1380-1383.
[18]雷泽宝,林建军.肺炎支原体抗体IgM、肺炎衣原体抗体IgM、嗜肺军团菌抗体IgM联合检测在儿童呼吸道感染诊治中的价值分析[J].中国妇幼保健,2015,30(35):6251-6253.
[2]王菲,李岩伟,黄小兰,等.肺炎衣原体及肺炎支原体临床血清学检测试剂的研发及应用[J].标记免疫分析与临床,2018,25(5):616-619.
[3]陈炎,丁红梅,张春梅.儿童肺炎支原体肺炎混合感染分析研究[J].宁夏医学杂志,2018,40(5):444-446.
[4]徐莉,王唯,李希辉.急性呼吸道感染患儿肺炎支原体和衣原体感染检测分析[J].中国病原生物学杂志,2017,12(9):893-896.
[5]姜梨梨,王瑞芳,周海群,等.亳州地区2846例儿童急性呼吸道感染病原体IgM抗体检测结果分析[J].重庆医学,2017,46(25):3569-3570,3573.
[6]夏厚才,彭惠轩,罗小兵,等.广州市南沙区儿童社区获得性肺炎病原调查及临床特点分析[J].中国实用医药,2017,12(20):6-8.
[7]张虹霞.呼吸道病原体九联检在儿童急性呼吸道感染中的临床应用[J].甘肃医药,2017,36(6):477,503.
[8]仝慧杰.呼吸道合胞病毒与肺炎支原体联合检测对儿童呼吸道病原菌筛查的应用效果[J].临床合理用药杂志,2018,11(14):150-151.
[9]朴春花.呼吸道感染患儿肺炎支原体和衣原体的检测及临床分析[J],中国现代医药杂志,2016,18(6):76-77.
[10]Converso TR,Goulart C,Rodriguez DM,et al.Systemic immunization with rPotD reduces Streptococcus pneumoniae nasopharyngeal colonization in mice[J].Vaccine,2017,35(1):149-155.
[11]陈秀娟,何兆英.儿童呼吸道感染者肺炎支原体、肺炎衣原体特异性抗体检测临床意义探讨[J].中国医学工程,2014,22(3):125.
[12]李晓,周春菊,邓燕妮,等.肺炎支原体抗体、C-反应蛋白及血清降钙素原检测在儿童呼吸道感染诊断中的应用评价[J].现代诊断与治疗,2018,29(14):2261-2263.
[13]孙静,马洪刚,祁百胜.儿童呼吸道感染检测降钙素原、肺炎衣原体抗体IgM和肺炎支原体抗体IgM的临床意义观察[J].中国中西医结合儿科学,2014,6(1):47-48.
[14]Head BM,Trajtman A,Rueda ZV,et al.Atypical bacterial pneumonia in the HIV-infected population[J].Pneumonia,2017,9(1):12.
[15]黎才丽,黄勇,许政衡.RSV与MP联合检测在住院儿童呼吸道病原菌中的筛查效果观察[J].河北医药,2018,40(17):2677-2679.
[16]刘大宁,张丽媛.荧光定量聚合酶链反应技术在儿童肺炎支原体及肺炎衣原体感染中的应用[J].实用医技杂志,2017,24(3):288-289.
[17]陈建辉.儿童呼吸道感染者肺炎支原体、肺炎衣原体特异性抗体检测临床意义探讨[J].中国卫生检验杂志,2008(7):1380-1383.
[18]雷泽宝,林建军.肺炎支原体抗体IgM、肺炎衣原体抗体IgM、嗜肺军团菌抗体IgM联合检测在儿童呼吸道感染诊治中的价值分析[J].中国妇幼保健,2015,30(35):6251-6253.