不同干燥方法对麦冬多元活性成分的影响
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摘要
探究不同干燥方法对麦冬多元活性成分的影响。采用超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定不同干燥方法下麦冬中甾体皂苷、高异黄酮、氨基酸及核苷类共29种活性成分的含量;根据29种目标成分的含量,用聚类分析(HCA)及主成分分析(PCA)对不同干燥方法下麦冬进行综合评价。结果表明,29种目标成分在一定浓度范围内线性关系良好,相关系数均大于0. 999 0;精密度、重复性、稳定性良好,RSD均小于5%;平均加样回收率为97. 02%～102. 78%,RSD均小于5%。PCA结果显示,以晒干、无硫烘干的麦冬样品综合质量较优。所建立的方法准确、可靠,可用于麦冬药材内在质量的综合评价,该研究可为揭示麦冬传统干燥方法科学内涵提供依据,同时为麦冬产地加工时干燥方法的优选提供基础资料。
To explore the effects of different drying methods on multiple bioactive constituents in Ophiopogonis Radix. A comprehensive analytical method employed with ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry( UFLC-QTRAP-MS/MS) was used for the simultaneous determination of twenty-nine components including steroidal saponins,homoisoflavonoids,amino acids and nucleosides in Ophiopogonis Radix. Hierarchical clustering analysis( HCA) and principal component analysis( PCA) have been applied to comprehensively analyze and evaluate the results of multiple active constituents. The results showed that twenty-nine components had good linearity,and correlation coefficients were more than 0. 999 0. The method exhibited good precision,repeatability and stability with the relative standard deviation( RSD) less than 5%,the average recoveries were between 97. 02% and 102. 78%,and all RSD were less than 5%. In addition,the results of PCA showed that sun drying and non-sulfurized oven drying samples have better quality. The established method was accurate and reliable,which could be used to appraise the quality of Ophiopogonis Radix. Our study can provide basis for revealing the scientific connotation of traditional drying method of Ophiopogonis Radix,and may lay the way for optimization of drying method during the processing of Ophiopogonis Radix.
To explore the effects of different drying methods on multiple bioactive constituents in Ophiopogonis Radix. A comprehensive analytical method employed with ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry( UFLC-QTRAP-MS/MS) was used for the simultaneous determination of twenty-nine components including steroidal saponins,homoisoflavonoids,amino acids and nucleosides in Ophiopogonis Radix. Hierarchical clustering analysis( HCA) and principal component analysis( PCA) have been applied to comprehensively analyze and evaluate the results of multiple active constituents. The results showed that twenty-nine components had good linearity,and correlation coefficients were more than 0. 999 0. The method exhibited good precision,repeatability and stability with the relative standard deviation( RSD) less than 5%,the average recoveries were between 97. 02% and 102. 78%,and all RSD were less than 5%. In addition,the results of PCA showed that sun drying and non-sulfurized oven drying samples have better quality. The established method was accurate and reliable,which could be used to appraise the quality of Ophiopogonis Radix. Our study can provide basis for revealing the scientific connotation of traditional drying method of Ophiopogonis Radix,and may lay the way for optimization of drying method during the processing of Ophiopogonis Radix.
引文
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2 Chinese Pharmacopoeia Commission.Pharmacopoeia of the People’s Republic of China:VolⅠ(中华人民共和国药典:第一部)[M].Beijing:China Medical Science Press,2015:155-156.
3 Lun W,Jiang XL,Zhang WM,et al.Homo-aro-cholestane,furostane and spirostane saponins from the tubers of Ophiopogon japonicas[J].Phytochemistry,2017,136:125-132.
4 Chen MH,Chen XJ,Wang M,et al.Ophiopogon japonicus-Aphytochemical,ethnomedicinal and pharmacological review[J].J Ethnopharmacol,2016,181:193-213.
5 Zhao JW,Chen DS,Deng CS,et al.Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas[J].BMC Complement Altern Med,2017,17(1):7-18.
6 Wang Y,Liu F,Liang Z,et al.Homoisoflavonoids and the antioxidant activity of Ophiopogon japonicus root[J].Iranian Jof Pharm Research Ijpr,2017,16:357-365.
7 Zhang HQ,Liu P,Zhu ZH,et al.Analysis and evaluation of amino acids and nucleosides in different parts of ripe fruit of Trichosanthes rosthornii harm[J].Mod Chin Med(中国现代中药),2017,19:1683-1687.
8 Wang GK,Zheng J,Yu Y,et al.Determination and evaluation of heavy metal elements in Kalimeris indica by ICP-MS coupled with principal component analysis and cluster analysis[J].Nat Prod Res Dev(天然产物研究与开发),2015,27:2084-2089.
9 Gao Q,Tang F,Sun J,et al.Simultaneous determination of nucleosides and amino acids in bamboo shoots by HPLC[J].Nat Prod Res Dev(天然产物研究与开发),2016,28:1409-1413.
10 Zhao RH,Duan JA,Gao ZJ,et al.Analysis and evaluation of traditional and modern drying technologies and methods of primary processing of traditional chinese medicinal materials[J].Mod Chin Med(中国现代中药),2013,15:1026-1035.
11 Duan JA,Su SL,Lv JL,et al.Traditional experiences and modern cognition on primary processing of traditional chinese medicinal materials[J].Chin Mater Med(中国中药杂志),2009,34:3151-3157.
12 Wu FM,Zhang FF,Li M,et al.Study on comprehensive evaluation of drying methods of sichuan Ophiopogon japonicus at production area[J].Chin Medicinal Mater(中药材),2015,38:1400-1402.
13 Wu FM,Zeng J,Li M,et al.Effect of drying temperature on quality of Ophiopogonis Radix based on the appearance character characteristics and content of chemical components[J].Chin J Pharm Anal(药物分析杂志),2016,36:1310-1315.
14 Xie M,Wang J,Zhang QL,et al.Correlation and principal component analyses of inorganic elements in Gentiana officinalis H.Smith.from different areas of Gansu province[J].Nat Prod Res Dev(天然产物研究与开发),2016,28:1402-1408.
15 Liu XY,Li JP,Chen XY,et al.Determination of eight active components in Radix Hedysari by HPLC and its cluster analysis[J].Acta Pharm Sin(药学学报),2016,51:786-791.