HPLC法同时测定石见穿中迷迭香酸和丹酚酸B的含量
|
摘要
目的建立HPLC法测定石见穿中迷迭香酸和丹酚酸B的含量。方法采用ZORBAX SB-C18柱(5μm,4.6×250mm),以乙腈-0.1%甲酸溶液(20∶80)为流动相,检测波长为286nm。结果迷迭香酸在0.0154～1.286μg,丹酚酸B在0.0132～1.103μg范围内进样量与峰面积呈良好的线性关系(r=1.0000),平均回收率分别为102.7%和101.5%,RSD分别为0.8%和1.7%。结论本方法简便快捷、重复性好,为石见穿药材质量标准的建立提供研究基础。
OBJECTIVE To establish a method for determining the content of rosmatinic acid and salvianolic B in Herba Salvia chinensis.METHODS The HPLC method was adopted,the separation was performed on a column ZORBAX SB-C18(5μm,4.6×250mm),acetonitrile-0.1% formic acid(20∶80) was used as the mobile phase,and the determination wavelength was at 286 nm.RESULTS Both rosmatinic acid and salvianolic B showed a good linearity at the range of 0.0154～1.286μg and 0.0132～1.103μg(r=1.0000),respectively;the average recoveries of rosmatinic acid was 102.7% with RSD 0.8%(n=6),whereas 101.5% for salvianolic B with RSD 1.7%(n=6).CONCLUSION The method was simple,accurate,reliable and repeatable;it can provide a basis for quality evaluating for Herba Salvia chinensis as medicinal materials.
OBJECTIVE To establish a method for determining the content of rosmatinic acid and salvianolic B in Herba Salvia chinensis.METHODS The HPLC method was adopted,the separation was performed on a column ZORBAX SB-C18(5μm,4.6×250mm),acetonitrile-0.1% formic acid(20∶80) was used as the mobile phase,and the determination wavelength was at 286 nm.RESULTS Both rosmatinic acid and salvianolic B showed a good linearity at the range of 0.0154～1.286μg and 0.0132～1.103μg(r=1.0000),respectively;the average recoveries of rosmatinic acid was 102.7% with RSD 0.8%(n=6),whereas 101.5% for salvianolic B with RSD 1.7%(n=6).CONCLUSION The method was simple,accurate,reliable and repeatable;it can provide a basis for quality evaluating for Herba Salvia chinensis as medicinal materials.
引文
[1]谢宗万.中华本草[M].第七册.上海:上海科学技术出版社,1999,162-164.
[2]国家药典委员会编.中国药典[S]. 1977年版.一部. 138-139.
[3]谢宗万.《本草纲目拾遗》石打穿与石见穿的品种考证[J].中国中药杂志,2000,25(1):49.
[4]郝近大,谢宗万.紫参古今名实考[J].中国中药杂志,1994,19(3):131-133.
[5]雷涛,孟繁钦,王艳芳.石见穿化学成分及药理研究进展[J].中国医药导报,2010,7(29):9-10,23.
[6]高俊峰,王秀辉,张鹏.石见穿化学成分和药理作用研究进展[J].中国实验方剂学杂志,2013,19(12):348-351.
[7]沙秀秀,戴新新,宿树兰,等.丹参茎叶药材的质量标准研究[J].药物分析杂志,2016,36(6):1094-1100.
[8]高俊峰,丁玲,张鹏,等.高效液相色谱法测定石见穿中迷迭香酸的含量[J].中华中医药杂志,2014,29(4):1192-1193.
[9]高俊峰,丁玲,张鹏,等. HPLC法测定石见穿中丹酚酸B的含量[J].中国药房,2014,25(7):639-640.
[10]王紫燕,李春沁,穆敏婕,等. HPLC波长切换法同时测定补肾活血方中丹参素、葛根素、大豆苷和丹酚酸B的含量[J].药物分析杂志,2016,36(6):1020-1026.
[2]国家药典委员会编.中国药典[S]. 1977年版.一部. 138-139.
[3]谢宗万.《本草纲目拾遗》石打穿与石见穿的品种考证[J].中国中药杂志,2000,25(1):49.
[4]郝近大,谢宗万.紫参古今名实考[J].中国中药杂志,1994,19(3):131-133.
[5]雷涛,孟繁钦,王艳芳.石见穿化学成分及药理研究进展[J].中国医药导报,2010,7(29):9-10,23.
[6]高俊峰,王秀辉,张鹏.石见穿化学成分和药理作用研究进展[J].中国实验方剂学杂志,2013,19(12):348-351.
[7]沙秀秀,戴新新,宿树兰,等.丹参茎叶药材的质量标准研究[J].药物分析杂志,2016,36(6):1094-1100.
[8]高俊峰,丁玲,张鹏,等.高效液相色谱法测定石见穿中迷迭香酸的含量[J].中华中医药杂志,2014,29(4):1192-1193.
[9]高俊峰,丁玲,张鹏,等. HPLC法测定石见穿中丹酚酸B的含量[J].中国药房,2014,25(7):639-640.
[10]王紫燕,李春沁,穆敏婕,等. HPLC波长切换法同时测定补肾活血方中丹参素、葛根素、大豆苷和丹酚酸B的含量[J].药物分析杂志,2016,36(6):1020-1026.