基于网络药理学的金银花活性成分抗炎作用机制的研究
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摘要
目的探讨金银花预防、治疗炎症的药效物质基础和作用机制。方法通过对scifinder数据库文献检索,整理获得金银花已报道的所有化学成分,并寻找与炎症相关的潜在靶点,采用网络药理学的方法对金银花药效物质作用机制进行研究,以金银花的94种活性成分和23种抗炎靶点蛋白为网络节点构建"分子-靶点"网络,并对23种抗炎靶点的分子进行基于String数据库的蛋白相互作用分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的通路富集分析。结果与结论通过分析,筛选出11个度值较高的候选活性分子和11个度值较高的抗炎蛋白。其中度值较高的候选化合物分子包括芦丁(J106)、lonijaposide Q(J111)、madreselvin B(J4)、secologanicdibutylacetal(J3)、3,5-dicaffeoylquinic acid buthyl ester(J80)等11个化合物。度值较高的靶点蛋白为IRAK4、PDE3B、PDE2A、PDE1B、PPARD、ADAM8、TOP2A、HSP90AA1、PDE4B、HSP90AB1、EIF5A。本研究初步研究了金银花抗炎的药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。综合网络分析结果显示,金银花抗炎活性呈现多分子、多靶点和多通路的特点,可为未来深入研究金银花的抗炎作用机制提供科学依据。
In order to explore the pharmacodynamic basis and mechanism of action of Lonicera japonica in preventing and treating inflammation,we used network pharmacology to study the mechanism of active ingredients of Lonicera japonica and constructed a "component-target" network with 94 active components of Lonicera japonica and 23 anti-inflammatory target proteins which were analyzed by the interaction analysis of the String database and the path enrichment analysis of the Kyoto encyclopedia of genes and genomes(KEGG).Finally,eleven candidate active molecules and eleven anti-inflammatory proteins with higher degree were screened,respectively.This study preliminarily verified the pharmacological effect and mechanism of anti-inflammation of Lonicera japonica,which could provide a scientific basis for the study of the anti-inflammatory mechanism of Lonicera japonica.
In order to explore the pharmacodynamic basis and mechanism of action of Lonicera japonica in preventing and treating inflammation,we used network pharmacology to study the mechanism of active ingredients of Lonicera japonica and constructed a "component-target" network with 94 active components of Lonicera japonica and 23 anti-inflammatory target proteins which were analyzed by the interaction analysis of the String database and the path enrichment analysis of the Kyoto encyclopedia of genes and genomes(KEGG).Finally,eleven candidate active molecules and eleven anti-inflammatory proteins with higher degree were screened,respectively.This study preliminarily verified the pharmacological effect and mechanism of anti-inflammation of Lonicera japonica,which could provide a scientific basis for the study of the anti-inflammatory mechanism of Lonicera japonica.
引文
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[2] 兰华,高丹,申鸿.金银花提取液抗氧化酶活性与清除自由基能力的研究[J].中国食品学报,2007,7(2):27-32.
[3] YU Y,SONG W X,ZHU C G.Hemosecoiridoids from the flower buds of Lonicera japonica[J].J Nat Prod,201l,74(10):2151-2160.
[4] 贺伟.金银花的化学成分及药理作用研究[J].中国医药导报,2007,4(24):8.[5] 李娜,张新庄,王俨如.基于网络药理学的通塞脉片治疗耳硬化症的作用机制[J].中国中药杂志,2016,9:1706-1712.
[6] 林卫东,胡靖敏,梁生旺,等.葛根改善胰岛素抵抗的网络药理学研究[J].中药材,2016,7:1628-1632.
[7] 芦丁,朱少晖,方月琴.菊科黄酮类化合物生物活性研究进展[J].学术探讨,2014,7:181-182.
[8] 郭严.刺山柑外敷治疗关节炎有效成分与致敏成分的分离[D].石河子:石河子大学,2012.
[9] 杜芳黎,姚彩云,宋志军.忍冬属植物中环烯醚萜苷类化合物的研究进展[J].天然产物研究与开发,2015,27(7):1296-1307.
[10] 宋亚玲,王红梅,倪付勇,等.金银花中酚酸类成分及其抗炎活性研究[J].中草药,2015,46(4):490-495.
[11] 文海平.白细胞介素-1受体相关激酶家族与自身免疫性疾病[J].医学研究生学报,2011,24(6):78-81.
[12] BEUTE J,LUKKES M,KOEKOEK P.A pathophy-siological role of PDE3 in allergic airway inflammation[J].JCI Insight,2018,3(2):1-16.
[13] 李小明,胡爱虹.磷酸二酯酶抑制剂的药理及临床应用进展[J].安徽医药,2006,10(6):464-465.
[14] PURUSHOTHAMAN B,ARUMUGAM P,SONG J M.A novel catecholopyrimidine based small molecule PDE4B inhibitor suppresses inflammatory cytokines in atopic mice[J].Front Pharmacol,2018,9:223-236.