牛初乳安全性的毒理学研究
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摘要
目的:评价牛初乳的安全性。方法:按照《保健食品检验与评价技术规范(2003版)》进行急性经口毒性试验、Ames试验、骨髓细胞微核试验、精子畸形试验和大鼠30d喂养试验等毒理学安全评价试验。结果:牛初乳大鼠经口急性毒性试验中MTD值>15. 0g/kg·BW。小鼠骨髓微核试验、Ames试验、小鼠精子致畸试验对牛初乳样品没有致突变的作用。大鼠30d喂养试验各项指标也均未见明显毒性反应:对照组和各剂量组大鼠的生长发育均未见异常,血液学、血液生化、主要脏器重量、脏器系数均在正常值范围内,组织病理学检查未见与受试物有关的异常改变。结论:牛初乳属于无毒级、不会引起突变,大鼠30d喂养试验各项指标也均未见明显毒性反应。
Objective To evaluate the toxicology safety of cow colostrum. Method Acute oral toxicity test,genetic toxicity test and 30 days oral toxicity test were conducted according to technical guidlines of inspection and evaluation for health food. Result MID of acute oral toxicity test for cow colostrum was greater than 15. 0 g/kg·BW. No mutation effects were induced in Ames test,bone marrow cell micronucleus test in mice,and sperm abnormality test in mice. For the 30 days oral toxicity test,the parameters including growth and development,hematology,biochemistry,main organ weight and organ weight ratio were in the range of normal value,and no abnormality related to the cow colostrum was induced in the histopathology. Conclusion No obvious toxicity was induced in cow colostrum.
Objective To evaluate the toxicology safety of cow colostrum. Method Acute oral toxicity test,genetic toxicity test and 30 days oral toxicity test were conducted according to technical guidlines of inspection and evaluation for health food. Result MID of acute oral toxicity test for cow colostrum was greater than 15. 0 g/kg·BW. No mutation effects were induced in Ames test,bone marrow cell micronucleus test in mice,and sperm abnormality test in mice. For the 30 days oral toxicity test,the parameters including growth and development,hematology,biochemistry,main organ weight and organ weight ratio were in the range of normal value,and no abnormality related to the cow colostrum was induced in the histopathology. Conclusion No obvious toxicity was induced in cow colostrum.
引文
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[8]孙红梅,罗蓉,李冬梅.辅酶Q10和维生素E配伍的稳定性和食用安全性研究[J].中国食物与营养,2016,22(6):17-20.
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[2]赵岩.初乳素的免疫调节作用[J].草食家畜,2001(3):55-57.
[3]程光宇,唐梓进,吴京燕,等.活性初乳素对小鼠的抗衰老作用[J].南京师大学报(自然科学版),1996,19(3):69-72.
[4]江海涛,吴京燕,程光宇,等.初乳素预防化学性肝损伤的实验研究[J].中国乳品工业,2005,33(5):12-14.
[5]吴京燕,潘玉英,杨云峰,等.牛初乳冻干粉对酒精性肝损伤的预防作用[J].中国比较医学杂志,2007,17(1):33-35.
[6]江海涛,任源浩,陈勇,等.牛初乳冻干粉改善小鼠睡眠状况的实验研究[J].中国乳品工业,2006,34(12):29-30、45.
[7]胡居吾,陈俊杰,涂伟,等.乳铁蛋白牛初乳颗粒的毒理安全性研究[J].安徽农业科学,2012(12):7195-7198.
[8]孙红梅,罗蓉,李冬梅.辅酶Q10和维生素E配伍的稳定性和食用安全性研究[J].中国食物与营养,2016,22(6):17-20.
[9]陈雅静.关于“食品”含义的文献综述---新《食品安全法》第150条探讨[J].中国食物与营养,2017,23(5):17-19.