基于线粒体COⅠ、Cytb和COⅡ基因的中国草地螟不同地理种群遗传分化分析
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摘要
【目的】揭示中国草地螟Loxostege sticticalis不同地理种群的遗传分化程度。【方法】采用PCR技术扩增中国西北和华北地区草地螟11个地理种群的线粒体COⅠ,Cytb和COⅡ基因序列,基于其序列变异及单倍型贝叶斯系统发育树和单倍型网络图分析,探讨不同地理种群间的遗传距离、分子系统发生关系及遗传分化程度。【结果】草地螟11个地理种群的线粒体COⅠ,Cytb和COⅡ基因序列分别有24, 12和69个变异位点(分别占总序列的3.6%, 2.7%和8.8%),检测到的单倍型分别为22, 14和16个,单倍型多样度(Hd)分别为0.7600, 0.5842和0.7341,核苷酸平均差异度(K)分别为1.704, 0.752和3.997,不同单倍型间的遗传距离平均值分别为0.004, 0.005和0.013。总种群的Tajima's D和Fu's Fs值皆不显著,表明草地螟不同地理种群间的遗传分化不明显,群体大小稳定。根据各地理种群的单倍型建立的系统发育树和单倍型网络图表明,各单倍型散布在不同的地理种群中,无明显的地理分布格局。【结论】草地螟各地理种群的遗传距离与地理距离间不具有显著的相关性,其遗传分化不明显。
【Aim】 To investigate the genetic differentiation of different geographic populations of Loxostege sticticalis in China. 【Methods】 The mitochondrial COⅠ, Cytb and COⅡ sequences from 11 geographic populations of L. sticticalis in Northwest and North China were amplified by PCR, and the genetic distance, molecular phylogeny and degree of genetic differentiation were analyzed based on their sequence variation, Bayesian inference(BI) phylogenetic tree and haplotype network. 【Results】 The sequence fragments of mitochondrial COⅠ, Cytb and COⅡ from 11 geographic populations of L. sticticalis had 24, 12 and 69 variable sites, respectively(accounting for 3.6%, 2.7% and 8.8% of the total length, respectively), 22, 14 and 16 haplotypes with the haplotype diversity(Hd) of 0.7600, 0.5842 and 0.7341, respectively, and the average nucleotide difference(K) of 1.704, 0.752 and 3.997, respectively. The average genetic distance based on mitochondrial COI, Cytb and COII sequences between different haplotypes was 0.004, 0.005 and 0.013, respectively. Both the Tajima's D and Fu's Fs of the total population were not significant, suggesting that the genetic differentiation among different geographic populations of L. sticticalis is not significant and the population size remains stable. The phylogenetic tree and haplotype network based on the haplotypes showed that the haplotypes were highly mixed in different geographic populations, without clear geographical distribution pattern. 【Conclusion】 The genetic distance between geographic populations of L. sticticalis is not significantly correlated with their geographical distance, and there is no obvious genetic differentiation between these populations.
【Aim】 To investigate the genetic differentiation of different geographic populations of Loxostege sticticalis in China. 【Methods】 The mitochondrial COⅠ, Cytb and COⅡ sequences from 11 geographic populations of L. sticticalis in Northwest and North China were amplified by PCR, and the genetic distance, molecular phylogeny and degree of genetic differentiation were analyzed based on their sequence variation, Bayesian inference(BI) phylogenetic tree and haplotype network. 【Results】 The sequence fragments of mitochondrial COⅠ, Cytb and COⅡ from 11 geographic populations of L. sticticalis had 24, 12 and 69 variable sites, respectively(accounting for 3.6%, 2.7% and 8.8% of the total length, respectively), 22, 14 and 16 haplotypes with the haplotype diversity(Hd) of 0.7600, 0.5842 and 0.7341, respectively, and the average nucleotide difference(K) of 1.704, 0.752 and 3.997, respectively. The average genetic distance based on mitochondrial COI, Cytb and COII sequences between different haplotypes was 0.004, 0.005 and 0.013, respectively. Both the Tajima's D and Fu's Fs of the total population were not significant, suggesting that the genetic differentiation among different geographic populations of L. sticticalis is not significant and the population size remains stable. The phylogenetic tree and haplotype network based on the haplotypes showed that the haplotypes were highly mixed in different geographic populations, without clear geographical distribution pattern. 【Conclusion】 The genetic distance between geographic populations of L. sticticalis is not significantly correlated with their geographical distance, and there is no obvious genetic differentiation between these populations.
引文
Bandelt HJ,Forster P,R?hl A,1999.Median-joining networks for inferring intraspecific phylogenies.Mol.Biol.Evol.,16(1):37-48.
Belov VK,Shurovenkov YB,Taran NA,1979.Details of the development of the meadow moth in the RSFSR.Zash.Rast.,5:44-46.
Berezhkov RP,1936.L.sticticalis in the forest zone of western Siberia.Biol.Tomsk.,2:98-131.
Cao WJ,2006.Studies on Genetic Diversity and Migration Trajectory of Different Geographic Populations of the Meadow Moth,Loxostege sticticalis (Lepidoptera:Pyralidae).MSc Thesis,Yangzhou University,Yangzhou,Jiangsu.[曹卫菊,2006.草地螟不同地理种群的遗传多样性及迁飞轨迹分析.江苏扬州:扬州大学硕士学位论文]
Chen SC,Wang XQ,Peng P,Hu X,Duan XF,Lin Q,2015.Genetic differentiation of Empoasca vitis (G?the) (Hemiptera:Cicadellidae) among eleven populations based on 16S rRNA sequence.Southwest China J.Agric.Sci.,28(2):584-590.[陈世春,王晓庆,彭萍,胡翔,段小凤,林强,2015.基于16S rRNA基因序列的11个假眼小绿叶蝉地理种群遗传分化研究.西南农业学报,28(2):584-590]
Chen X,Jiang YY,Meng ZP,Chen K,Kang AG,Li CM,Zhai BP,2016.Extreme climate has become an important factor causing the termination of outbreak periods of Loxostege sticticalis (Lepidoptera:Pyralidae) in China.Acta Entomol.Sin.,59(12):1363-1375.[陈晓,姜玉英,孟正平,陈阔,康爱国,李春民,翟保平,2016.极端气候成为我国草地螟爆发周期终结的重要因子.昆虫学报,59(12):1363-1375]
Dong MS,Yang L,Chen XS,Zhang YJ,2017.Analysis of genetic diversity among different geographic populations of Anaka burmensis (Hemiptera:Cicadellidae) based on part of mtDNA COI gene sequences.Sichuan J.Zool.,36(3):277-283.[董梦书,杨琳,陈祥盛,张余杰,2017.基于线粒体COI基因部分序列的缅甸安小叶蝉地理种群遗传多样性研究.四川动物,36(3):277-283]
Excoffier L,Laval G,Schneider S,2007.Arlequin (version 3.0):an integrated software package for population genetics data analysis.Evol.Bioinform.Online,23(1):47-50.
Filatova TP,1935.The sugar-web worm Loxostege sticticalis L.in Karaganda District.Plant Prot.,4:111-122.
Folmer O,Black M,Hoeh W,Lutz R,Vrijenhoek R,1994.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.Mol.Mar.Biol.Biotechnol.,3(5):294 - 299.
Huelsenbeck JP,Ronquist F,2001.MRBAYES:Bayesian inference of phylogentic trees.Bioinformatics,17(8):754-755.
Jiang XF,Cao WJ,Zhang L,Luo LZ,2010.Beet webworm (Lepidoptera:Pyralidae) migration in China:evidence from genetic markers.Environ.Entomol.,39(1):232-242.
Kang AG,Fan RX,Zhang YH,Li Q,Zhang FY,Yang LJ,Zhao XJ,2003.Occurrence characteristics,factors and control measures of Loxostege sticticalis in the third occurrence period.Entomol.Knowl.,40(1):75-79.[康爱国,樊荣贤,张玉慧,李强,张凤英,杨立军,赵晓娟,2003.草地螟第三个暴发周期的发生特点、成因及防治对策.昆虫知识,40(1):75-79]
Knor IB,1990.The beet webworm in the Asian part of the USSR.Zash.Rast.,11:52-56.
Li J,Zhang Y,Wang ZY,He KL,Wang Q,2010.Genetic differentiation and gene flow among different geographical populations of the Asian corn borer,Ostrinia furnacalis (Guenée) (Lepidoptera:Crambidae) in China estimated by mitochondrial COII gene sequences.Acta Entomol.Sin.,53(10):1135-1143.[李菁,张颖,王振营,何康来,王强,2010.基于线粒体DNA COII基因的亚洲玉米螟中国不同地理种群遗传分化及基因流研究.昆虫学报,53(10):1135-1143]
Li R,Han LL,Wang H,Zhao KJ,Mang YL,Zhang HY,Fan D,2015.Analysis of genetic differentiation among different geographic populations of Aphis glycines (Hemiptera:Aphididae) based on mtDNA COII gene sequences.Chin.J.Appl.Entomol.,52(5):1203-1214.[李冉,韩岚岚,王红,赵奎军,莽逸伦,张红玉,樊东,2015.基于线粒体COII基因的大豆蚜不同地理种群遗传分化研究.应用昆虫学报,52(5):1203-1214]
Librado P,Rozas J,2009.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25:1451-1452.
Mamonov BA,1930.Observations on Loxostege sticticalis and the results of tests of the action of insecticides on cultivated oil plants.Bull.N.Caucas.Agric.Expt.Sta.,66:314.
Mikhal’tsov VP,1980.Tachinids and the meadow moth.Zash.Rast.,4:38.
National Scientific Research Cooperation Group of Loxostege sticticalis,1987.Research on occurrence,forecast and control of Loxostege sticticalis.Bingchong Cebao,(s1):1-9.[全国草地螟科研协作组,1987.草地螟(Loxostege sticticalis Linnaeus)发生及测报和防治的研究.病虫测报,(s1):1-9]
Omelyuta VP,Nadvornaya LS,1980.Parasites of the beet webworm Pyrausta sticticalis L.(Lepidoptera,Pyraustidae) in the Ukraine.Vest.Zool.,2:89-90.
Peakall R,Smouse PE,2006.GENALEX 6:genetic analysis in Excel.Population genetic software for teaching and research.Mol.Ecol.Notes,6:288-295.
Popov P,1976.Morphological and biological features of Pyrausta sticticalis.Rast.Zash.,24(4):33-38.
Rousset F,1997.Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance.Genetics,145(4):1219-1228.
Shu SS,Cui J,Zhu SY,Xu W,Wang XQ,2018.Genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera:Olethreutidae) based on mitochondrial COI gene sequences.J.Plant Prot.,45(2):214-222.[史树森,崔娟,朱诗禹,徐伟,王小奇,2018.基于线粒体COI基因序列的大豆食心虫不同地理种群遗传分化.植物保护学报,45(2):214-222]
Simmons RB,Weller SJ,2001.Utility and evolution of cytochrome b in insects.Mol.Phylogenet.Evol.,20(2):196-210.
Simon C,Frati F,Bekenbach A,Crespi B,Liu H,Flook P,1994.Evolution,weighting,and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers.Ann.Entomol.Soc.Am.,87(6):651-701.
Steinberg D,1935.The possibilities of the sugar beet webworm (Loxostege sticticalis L.) propagation in the virgin steppes of the Kalmuik Autonomous SSR.Bull.Plant Prot.,Leningrad,13:68.
Sun W,Zhang ZT,Dong H,Qian HT,Shi Y,Xie LN,Cong B,2013.Analysis of genetic differentiation and gene flow among different geographic populations of Oedaleus infernalis (Orthoptera:Acrididae) based on mtDNA COI gene sequences.Acta Entomol.Sin.,56(8):907-916.[孙嵬,张柱亭,董辉,钱海涛,石玉,谢丽娜,丛斌,2013.基于线粒体COI基因序列的黄胫小车蝗不同地理种群的遗传分化及基因流分析.昆虫学报,56(8):907-916]
Tajima F,1989.The effect of change in population size on DNA polymorphism.Genetics,123 (3):597-601.
Tamura K,Stecher G,Peterso D,Filipski A,Kumar S,2013.MEGA6:Molecular Evolutionary Genetics Analysis version 6.0.Mol.Biol.Evol.,30(12):2725-2729.
Timm AE,Pringle KL,Wamich L,2005.Genetic diversity of woolly apple aphid Eriosoma lanigerum (Hemiptera:Aphididae) populations in the Western Cape,South Africa.Bull.Entomol.Res.,95(3):187-191.
Wang KQ,Liu XL,Shao TY,2018.Analysis of the genetic diversity and host specialization of Ostrinia furnacalis (Lepidoptera:Crambidae) in Northeast China.Acta Entomol.Sin.,61(9):1054-1066.[王克勤,刘兴龙,邵天玉,2018.中国东北地区亚洲玉米螟遗传多样性及寄主专化性分析.昆虫学报,61(9):1054-1066]
Wang XY,Xu GQ,2014.Genetic differentiation and gene flow among geographic populations of Spodoptera exigua (Lepidoptera:Noctuidae) in China.Acta Entomol.Sin.,57(9):1061-1074.[王兴亚,许国庆,2014.中国甜菜夜蛾地理种群的遗传分化与基因流.昆虫学报,57(9):1061-1074]
Wang Y,Chen KP,Yao Q,2009.An introduction to the operation method of phylogenetic analysis program MrBayes 3.1.J.Anhui Agric.Sci.,37(33):16665-16669.[王勇,陈克平,姚勤,2009.系统发生分析程序MrBayes 3.1使用方法介绍.安徽农业科学,37(33):16665-16669]
Wei Q,Cui WL,Du JL,Sun MJ,Zhao XL,Gu CY,Liang YC,Zhang GZ,Li CX,Huang ZF,Jia ZY,1987.Research on occurrence law,forecast and integrated control of Loxostege sticticalis in Heilongjiang province during 1981-1985.Bingchong Cebao,(s1):98-107.[魏倩,崔万里,杜俊岭,孙明江,赵晓丽,顾成玉,梁艳春,张广芝,李长祥,黄自芳,贾宗谊,1987.黑龙江省草地螟(Loxostege sticticalis)发生规律、预测预报及综合防治研究(1981-1985年).病虫测报,(s1):98-107]
Yang RS,Zhong L,Jiang YR,Shi SL,Liu YQ,Xia RX,Qin L,2011.Genetic diversity and phylogenetic analysis of COI genes from Curculio arakawai weevil and 24 other insect species.Sci.Seric.,37(6):985-992.[杨瑞生,钟亮,姜义仁,石生林,刘彦群,夏润玺,秦利,2011.橡实象虫等25种昆虫线粒体COI基因的遗传多样性及系统进化分析.蚕业科学,37(6):985-992]
Yang SY,Zhou XL,Chen LL,Zhang XH,Yang HY,Shen HM,2018.The genetic analysis of two-spotted spider mite Tetranychus urticae geographical populations in Gansu Province.J.Plant Prot.,45(6):1328-1334.[杨顺义,周兴隆,陈露露,张新虎,杨航宇,沈慧敏,2018.甘肃省二斑叶螨地理种群的遗传分析.植物保护学报,45(6):1328-1334]
Yue ZD,Yuan Y,1983.Preliminary analysis on insect source and occurrence condition of Loxostege sticticalis in Jilin province.Jilin Agric.Sci.,(3):78-81.[岳宗岱,袁艺,1983.吉林省草地螟虫源和发生条件的初步分析.吉林农业科学,(3):78-81]
Zhang SK,Liu MF,Li QR,Li JQ,1987.Research on occurrence law,forecast and integrated control of Loxostege sticticalis in Shanxi province.Bingchong Cebao,(s1):82-97.[张树坤,刘梅凤,李齐仁,李吉庆,1987.山西省草地螟发生规律、 预测预报及其综合防治的研究.病虫测报,(s1):82-97]
Zhao AP,Sun L,Wang MR,Jia SF,Ma GS,2017.Monitoring and population dynamics analysis of six agricultural pest species in Daxing District.China J.Plant Prot.,37(10):38-45.[赵安平,孙璐,王美人,贾淑芬,马国生,2017.大兴区6种农业害虫灯诱监测与种群动态分析.中国植保导刊,37(10):38-45]
Zhao J,Hu SJ,He QJ,Yi CH,He J,Feng ZW,Yang JH,Chen P,2017.Genetic diversity of Bhutanitis thaidina Blanchard,based on mitochondrial COI and ND1.J.Fujian Agric.For.Univ.(Nat.Sci.Ed.),46(4):387-391.[赵健,胡劭骥,和秋菊,易传辉,和菊,冯志伟,杨建华,陈鹏,2017.基于线粒体COI和ND1基因分析三尾凤蝶遗传多样性.福建农林大学学报(自然科学版),46(4):387-391]
Belov VK,Shurovenkov YB,Taran NA,1979.Details of the development of the meadow moth in the RSFSR.Zash.Rast.,5:44-46.
Berezhkov RP,1936.L.sticticalis in the forest zone of western Siberia.Biol.Tomsk.,2:98-131.
Cao WJ,2006.Studies on Genetic Diversity and Migration Trajectory of Different Geographic Populations of the Meadow Moth,Loxostege sticticalis (Lepidoptera:Pyralidae).MSc Thesis,Yangzhou University,Yangzhou,Jiangsu.[曹卫菊,2006.草地螟不同地理种群的遗传多样性及迁飞轨迹分析.江苏扬州:扬州大学硕士学位论文]
Chen SC,Wang XQ,Peng P,Hu X,Duan XF,Lin Q,2015.Genetic differentiation of Empoasca vitis (G?the) (Hemiptera:Cicadellidae) among eleven populations based on 16S rRNA sequence.Southwest China J.Agric.Sci.,28(2):584-590.[陈世春,王晓庆,彭萍,胡翔,段小凤,林强,2015.基于16S rRNA基因序列的11个假眼小绿叶蝉地理种群遗传分化研究.西南农业学报,28(2):584-590]
Chen X,Jiang YY,Meng ZP,Chen K,Kang AG,Li CM,Zhai BP,2016.Extreme climate has become an important factor causing the termination of outbreak periods of Loxostege sticticalis (Lepidoptera:Pyralidae) in China.Acta Entomol.Sin.,59(12):1363-1375.[陈晓,姜玉英,孟正平,陈阔,康爱国,李春民,翟保平,2016.极端气候成为我国草地螟爆发周期终结的重要因子.昆虫学报,59(12):1363-1375]
Dong MS,Yang L,Chen XS,Zhang YJ,2017.Analysis of genetic diversity among different geographic populations of Anaka burmensis (Hemiptera:Cicadellidae) based on part of mtDNA COI gene sequences.Sichuan J.Zool.,36(3):277-283.[董梦书,杨琳,陈祥盛,张余杰,2017.基于线粒体COI基因部分序列的缅甸安小叶蝉地理种群遗传多样性研究.四川动物,36(3):277-283]
Excoffier L,Laval G,Schneider S,2007.Arlequin (version 3.0):an integrated software package for population genetics data analysis.Evol.Bioinform.Online,23(1):47-50.
Filatova TP,1935.The sugar-web worm Loxostege sticticalis L.in Karaganda District.Plant Prot.,4:111-122.
Folmer O,Black M,Hoeh W,Lutz R,Vrijenhoek R,1994.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.Mol.Mar.Biol.Biotechnol.,3(5):294 - 299.
Huelsenbeck JP,Ronquist F,2001.MRBAYES:Bayesian inference of phylogentic trees.Bioinformatics,17(8):754-755.
Jiang XF,Cao WJ,Zhang L,Luo LZ,2010.Beet webworm (Lepidoptera:Pyralidae) migration in China:evidence from genetic markers.Environ.Entomol.,39(1):232-242.
Kang AG,Fan RX,Zhang YH,Li Q,Zhang FY,Yang LJ,Zhao XJ,2003.Occurrence characteristics,factors and control measures of Loxostege sticticalis in the third occurrence period.Entomol.Knowl.,40(1):75-79.[康爱国,樊荣贤,张玉慧,李强,张凤英,杨立军,赵晓娟,2003.草地螟第三个暴发周期的发生特点、成因及防治对策.昆虫知识,40(1):75-79]
Knor IB,1990.The beet webworm in the Asian part of the USSR.Zash.Rast.,11:52-56.
Li J,Zhang Y,Wang ZY,He KL,Wang Q,2010.Genetic differentiation and gene flow among different geographical populations of the Asian corn borer,Ostrinia furnacalis (Guenée) (Lepidoptera:Crambidae) in China estimated by mitochondrial COII gene sequences.Acta Entomol.Sin.,53(10):1135-1143.[李菁,张颖,王振营,何康来,王强,2010.基于线粒体DNA COII基因的亚洲玉米螟中国不同地理种群遗传分化及基因流研究.昆虫学报,53(10):1135-1143]
Li R,Han LL,Wang H,Zhao KJ,Mang YL,Zhang HY,Fan D,2015.Analysis of genetic differentiation among different geographic populations of Aphis glycines (Hemiptera:Aphididae) based on mtDNA COII gene sequences.Chin.J.Appl.Entomol.,52(5):1203-1214.[李冉,韩岚岚,王红,赵奎军,莽逸伦,张红玉,樊东,2015.基于线粒体COII基因的大豆蚜不同地理种群遗传分化研究.应用昆虫学报,52(5):1203-1214]
Librado P,Rozas J,2009.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25:1451-1452.
Mamonov BA,1930.Observations on Loxostege sticticalis and the results of tests of the action of insecticides on cultivated oil plants.Bull.N.Caucas.Agric.Expt.Sta.,66:314.
Mikhal’tsov VP,1980.Tachinids and the meadow moth.Zash.Rast.,4:38.
National Scientific Research Cooperation Group of Loxostege sticticalis,1987.Research on occurrence,forecast and control of Loxostege sticticalis.Bingchong Cebao,(s1):1-9.[全国草地螟科研协作组,1987.草地螟(Loxostege sticticalis Linnaeus)发生及测报和防治的研究.病虫测报,(s1):1-9]
Omelyuta VP,Nadvornaya LS,1980.Parasites of the beet webworm Pyrausta sticticalis L.(Lepidoptera,Pyraustidae) in the Ukraine.Vest.Zool.,2:89-90.
Peakall R,Smouse PE,2006.GENALEX 6:genetic analysis in Excel.Population genetic software for teaching and research.Mol.Ecol.Notes,6:288-295.
Popov P,1976.Morphological and biological features of Pyrausta sticticalis.Rast.Zash.,24(4):33-38.
Rousset F,1997.Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance.Genetics,145(4):1219-1228.
Shu SS,Cui J,Zhu SY,Xu W,Wang XQ,2018.Genetic differentiation among geographic populations of Leguminivora glycinivorella (Lepidoptera:Olethreutidae) based on mitochondrial COI gene sequences.J.Plant Prot.,45(2):214-222.[史树森,崔娟,朱诗禹,徐伟,王小奇,2018.基于线粒体COI基因序列的大豆食心虫不同地理种群遗传分化.植物保护学报,45(2):214-222]
Simmons RB,Weller SJ,2001.Utility and evolution of cytochrome b in insects.Mol.Phylogenet.Evol.,20(2):196-210.
Simon C,Frati F,Bekenbach A,Crespi B,Liu H,Flook P,1994.Evolution,weighting,and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers.Ann.Entomol.Soc.Am.,87(6):651-701.
Steinberg D,1935.The possibilities of the sugar beet webworm (Loxostege sticticalis L.) propagation in the virgin steppes of the Kalmuik Autonomous SSR.Bull.Plant Prot.,Leningrad,13:68.
Sun W,Zhang ZT,Dong H,Qian HT,Shi Y,Xie LN,Cong B,2013.Analysis of genetic differentiation and gene flow among different geographic populations of Oedaleus infernalis (Orthoptera:Acrididae) based on mtDNA COI gene sequences.Acta Entomol.Sin.,56(8):907-916.[孙嵬,张柱亭,董辉,钱海涛,石玉,谢丽娜,丛斌,2013.基于线粒体COI基因序列的黄胫小车蝗不同地理种群的遗传分化及基因流分析.昆虫学报,56(8):907-916]
Tajima F,1989.The effect of change in population size on DNA polymorphism.Genetics,123 (3):597-601.
Tamura K,Stecher G,Peterso D,Filipski A,Kumar S,2013.MEGA6:Molecular Evolutionary Genetics Analysis version 6.0.Mol.Biol.Evol.,30(12):2725-2729.
Timm AE,Pringle KL,Wamich L,2005.Genetic diversity of woolly apple aphid Eriosoma lanigerum (Hemiptera:Aphididae) populations in the Western Cape,South Africa.Bull.Entomol.Res.,95(3):187-191.
Wang KQ,Liu XL,Shao TY,2018.Analysis of the genetic diversity and host specialization of Ostrinia furnacalis (Lepidoptera:Crambidae) in Northeast China.Acta Entomol.Sin.,61(9):1054-1066.[王克勤,刘兴龙,邵天玉,2018.中国东北地区亚洲玉米螟遗传多样性及寄主专化性分析.昆虫学报,61(9):1054-1066]
Wang XY,Xu GQ,2014.Genetic differentiation and gene flow among geographic populations of Spodoptera exigua (Lepidoptera:Noctuidae) in China.Acta Entomol.Sin.,57(9):1061-1074.[王兴亚,许国庆,2014.中国甜菜夜蛾地理种群的遗传分化与基因流.昆虫学报,57(9):1061-1074]
Wang Y,Chen KP,Yao Q,2009.An introduction to the operation method of phylogenetic analysis program MrBayes 3.1.J.Anhui Agric.Sci.,37(33):16665-16669.[王勇,陈克平,姚勤,2009.系统发生分析程序MrBayes 3.1使用方法介绍.安徽农业科学,37(33):16665-16669]
Wei Q,Cui WL,Du JL,Sun MJ,Zhao XL,Gu CY,Liang YC,Zhang GZ,Li CX,Huang ZF,Jia ZY,1987.Research on occurrence law,forecast and integrated control of Loxostege sticticalis in Heilongjiang province during 1981-1985.Bingchong Cebao,(s1):98-107.[魏倩,崔万里,杜俊岭,孙明江,赵晓丽,顾成玉,梁艳春,张广芝,李长祥,黄自芳,贾宗谊,1987.黑龙江省草地螟(Loxostege sticticalis)发生规律、预测预报及综合防治研究(1981-1985年).病虫测报,(s1):98-107]
Yang RS,Zhong L,Jiang YR,Shi SL,Liu YQ,Xia RX,Qin L,2011.Genetic diversity and phylogenetic analysis of COI genes from Curculio arakawai weevil and 24 other insect species.Sci.Seric.,37(6):985-992.[杨瑞生,钟亮,姜义仁,石生林,刘彦群,夏润玺,秦利,2011.橡实象虫等25种昆虫线粒体COI基因的遗传多样性及系统进化分析.蚕业科学,37(6):985-992]
Yang SY,Zhou XL,Chen LL,Zhang XH,Yang HY,Shen HM,2018.The genetic analysis of two-spotted spider mite Tetranychus urticae geographical populations in Gansu Province.J.Plant Prot.,45(6):1328-1334.[杨顺义,周兴隆,陈露露,张新虎,杨航宇,沈慧敏,2018.甘肃省二斑叶螨地理种群的遗传分析.植物保护学报,45(6):1328-1334]
Yue ZD,Yuan Y,1983.Preliminary analysis on insect source and occurrence condition of Loxostege sticticalis in Jilin province.Jilin Agric.Sci.,(3):78-81.[岳宗岱,袁艺,1983.吉林省草地螟虫源和发生条件的初步分析.吉林农业科学,(3):78-81]
Zhang SK,Liu MF,Li QR,Li JQ,1987.Research on occurrence law,forecast and integrated control of Loxostege sticticalis in Shanxi province.Bingchong Cebao,(s1):82-97.[张树坤,刘梅凤,李齐仁,李吉庆,1987.山西省草地螟发生规律、 预测预报及其综合防治的研究.病虫测报,(s1):82-97]
Zhao AP,Sun L,Wang MR,Jia SF,Ma GS,2017.Monitoring and population dynamics analysis of six agricultural pest species in Daxing District.China J.Plant Prot.,37(10):38-45.[赵安平,孙璐,王美人,贾淑芬,马国生,2017.大兴区6种农业害虫灯诱监测与种群动态分析.中国植保导刊,37(10):38-45]
Zhao J,Hu SJ,He QJ,Yi CH,He J,Feng ZW,Yang JH,Chen P,2017.Genetic diversity of Bhutanitis thaidina Blanchard,based on mitochondrial COI and ND1.J.Fujian Agric.For.Univ.(Nat.Sci.Ed.),46(4):387-391.[赵健,胡劭骥,和秋菊,易传辉,和菊,冯志伟,杨建华,陈鹏,2017.基于线粒体COI和ND1基因分析三尾凤蝶遗传多样性.福建农林大学学报(自然科学版),46(4):387-391]
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