副猪嗜血杆菌thyA基因的序列分析
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摘要
为探究副猪嗜血杆菌thyA基因的遗传特性,针对于分离的副猪嗜血杆菌辽宁分离株的thyA基因进行测序,并运用软件对基因序列及氨基酸序列的同源性等进行分析研究。结果显示,thyA基因在副猪嗜血杆菌辽宁株亲缘性较高,其中基因序列与已公布的参考菌株同源性为95.7%~100%,氨基酸序列同源性为97.2%~100%,预测thyA基因编码蛋白的三级结构,发现thyA毒力基因在副猪嗜血杆菌中保守存在,为揭示thyA基因在副猪嗜血杆菌中功能奠定了一定基础。
To investigate the genetic characteristics of the thyA gene of Haemophilus parasuis,the thyA gene of Haemophilus parasuis isolate in Liaoning was sequenced,and the homology analysis of the gene sequence and amino acid sequence was performed using software.The results showed that the thyA gene was highly related to the strain of Haemophilus parasuis in Liaoning,and the homology of the gene sequence to the published reference strain was 95.7%-100%,and the amino acid sequence homology was from 97.2%to 100%.The analysis predicted the tertiary structure of the protein encoded by the thyA gene.The thyA virulence gene was found to be conserved in Haemophilus parasuis,laying a foundation for revealing the function of thyA gene in Haemophilus parasuis.
To investigate the genetic characteristics of the thyA gene of Haemophilus parasuis,the thyA gene of Haemophilus parasuis isolate in Liaoning was sequenced,and the homology analysis of the gene sequence and amino acid sequence was performed using software.The results showed that the thyA gene was highly related to the strain of Haemophilus parasuis in Liaoning,and the homology of the gene sequence to the published reference strain was 95.7%-100%,and the amino acid sequence homology was from 97.2%to 100%.The analysis predicted the tertiary structure of the protein encoded by the thyA gene.The thyA virulence gene was found to be conserved in Haemophilus parasuis,laying a foundation for revealing the function of thyA gene in Haemophilus parasuis.
引文
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[15] Rodríguez-Arce I,MartíS,Euba B.Inactivation of the thymidylate synthase thyA in nontypeable Haemophilus influenzae modulates antibiotic resistance and has a strong impact on its interplay with the host airways[J].Front Cell Infect Microbiol,2017,266:7-20.
[16] Ikuo M,Kaito C,Sekimizu K.The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene[J].Microb Pathog,2010,49(1-2):1-7.
[17] Chatterjee I,Kriegeskorte A,Fischer A,et al.In vivo mutations of thymidylate synthase(encoded by thyA)are responsible for thymidine dependency in clinical small-colony variants of Staphylococcus aureus[J].J Bacteriol,2008,190:834-842.
[18] Zhou S M,Xu C G,Zhang B,et al.Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2mutant of Haemophilus parasuis SC096strain via the classical complement pathway[J].Vet J,2013 196(1):111-113.
[2] Oliveira S,Pijoan C.Haemophilus parasuis:new trends on diagnosis,epidemiology and control[J].Vet Microbiol,2004,99:1-12.
[3] Ma B,Hua K,Zhou S.Haemophilus parasuis infection activates NOD1/2-RIP2signaling pathway in PK-15cells[J].Dev Comp Immunol,2018,79:158-165.
[4] Costa-Hurtado M,Olvera A,Martinez-Moliner V,et al.Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence[J].Infect Immun,2013,81(7):2327-2333.
[5]覃一峰,覃念.蓝耳病病毒和副猪嗜血杆菌病混合感染病例的诊断与分析[J].黑龙江畜牧兽医,2017(22):105-107.
[6]余远迪,曾泽,岳华,等.副猪嗜血杆菌和猪传染性胸膜肺炎放线杆菌双重PCR检测方法的建立及应用[J].动物医学进展,2013,34(12):29-33.
[7] Mathieu-Denoncourt A,Letendre C,Auger J P.Limited interactions between Streptococcus suis and Haemophilus parasuis in vitro co-infection studies[J].Pathogens,2018,7(1):6.
[8] Ling Z,Yonghong L,Junfeng L.Tilmicosin-and florfenicolloaded hydrogenated castor oil-solid lipid nanoparticles to pigs:Combined antibacterial activities and pharmacokinetics[J].Vet Pharmacol Ther,2017,41(2):1229-1234.
[9] Zhou X L,Xu X J,Zhao Y X,et al.Distribution of antimicrobial resistance among different serovars of Haemophilus parasuis isolates[J].Vet Microbiol,2010,141:168-173.
[10] Wen Y.Oxy R of Haemophilus parasuis is a global transcriptional regulator important in oxidative stress resistance and growth[J].Gene,2018,15(643):107-116.
[11] Costahurtado M,Aragon V.Advances in the quest for virulence factors of Haemophilus parasuis[J].Vet J,2013,198:571-576.
[12] Oliveria S,Galina L,Pijoan C.Development of a PCR test to diagnose Haemophilus parasuis infections[J].J Vet Diagn Invest,2001,13(6):495-501.
[13]王超,陈欣,黄晨,等.副猪嗜血杆菌的分离鉴定及其OmpP5基因序列分析[J].动物医学进展,2018,39(1):16-21.
[14] McDowall R,Durda Slavic D,MacInnes J I.Evaluation of a real-time polymerase chain reaction assay of the outer membrane protein P2gene for the detection of Haemophilus parasuis in clinical samples[J].Can J Vet Res,2014,78(2):150-152.
[15] Rodríguez-Arce I,MartíS,Euba B.Inactivation of the thymidylate synthase thyA in nontypeable Haemophilus influenzae modulates antibiotic resistance and has a strong impact on its interplay with the host airways[J].Front Cell Infect Microbiol,2017,266:7-20.
[16] Ikuo M,Kaito C,Sekimizu K.The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene[J].Microb Pathog,2010,49(1-2):1-7.
[17] Chatterjee I,Kriegeskorte A,Fischer A,et al.In vivo mutations of thymidylate synthase(encoded by thyA)are responsible for thymidine dependency in clinical small-colony variants of Staphylococcus aureus[J].J Bacteriol,2008,190:834-842.
[18] Zhou S M,Xu C G,Zhang B,et al.Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2mutant of Haemophilus parasuis SC096strain via the classical complement pathway[J].Vet J,2013 196(1):111-113.