3株禽滑液囊支原体分离株致病性的比较和评价（英文）
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摘要
【背景】禽滑液囊支原体是感染鸡的一种重要病原体,给我国的家禽养殖业带来了严重的经济损失。【目的】评价从病鸡中分离到的3株禽滑液囊支原体的致病性,以期丰富不同区域来源的分离株对鸡致病性的认识。【方法】分别用分离株CHN-WF224-2016、CHN-BZJ2-2015、CHN-JNB19-2016感染商品肉鸡,并在攻毒后第10天和21天进行实验室解剖,通过气囊和足垫的临床病变、血清学检测结果及气管粘膜病理形态学改变来比较评价分离株的致病力。【结果】CHN-BZJ2-2015和CHN-WF224-2016分离株能引起明显足垫肿胀和关节滑膜炎,其中CHN-BZJ2-2015分离株致病力最强,感染21d后的血清学检测和气管粘膜厚度与CHN-JNB19-2016分离株和MS-H疫苗株比较差异显著(P<0.05)。【结论】本研究表明我国目前流行的禽滑液囊支原体菌株其致病力存在明显差异,强调养殖场要积极控制和预防禽滑液囊支原体感染的重要性,同时为今后该疫苗的研发积累了实验数据。
[Background] Mycoplasma synoviae(MS) is an important causative agent infecting chicken, which causes enormous losses to the poultry industry in China. [Objective] This study was designed to evaluate the pathogenicity of three isolates from MS outbreak farms, which could enrich our understanding about pathogenicity of MS isolates from different districts. [Methods] Systemic MS infection was induced experimentally in commercial broiler chickens with recent MS isolates(CHN-WF224-2016, CHN-BZJ2-2015 and CHN-JNB19-2016). The virulence of each strain was evaluated by detecting air sac and foot pad lesions, serologic response, tracheal mucosal thickness and MS isolation rates from the infected chicken at 10 and 21 days post-infection and comparing these results with those obtained from a live attenuated vaccine strain(MS-H) and uninfected controls. [Results] Isolates CHN-BZJ2-2015 and CHN-WF224-2016 induced foot pad lesions and typical infectious synovitis, especially CHN-BZJ2-2015 group. Serologic response and tracheal mucosal thickness measurements at 21 days post infection indicated that CHN-BZJ2-2015 isolate was significantly more virulent than the CHN-JNB19-2016 isolate and MS-H strain(P<0.05). [Conclusion] The variation in virulence of MS isolates emphasizes the importance of active, on-going control and prevention of MS in the chicken flocks of China and accumulates experimental data for vaccine development.
[Background] Mycoplasma synoviae(MS) is an important causative agent infecting chicken, which causes enormous losses to the poultry industry in China. [Objective] This study was designed to evaluate the pathogenicity of three isolates from MS outbreak farms, which could enrich our understanding about pathogenicity of MS isolates from different districts. [Methods] Systemic MS infection was induced experimentally in commercial broiler chickens with recent MS isolates(CHN-WF224-2016, CHN-BZJ2-2015 and CHN-JNB19-2016). The virulence of each strain was evaluated by detecting air sac and foot pad lesions, serologic response, tracheal mucosal thickness and MS isolation rates from the infected chicken at 10 and 21 days post-infection and comparing these results with those obtained from a live attenuated vaccine strain(MS-H) and uninfected controls. [Results] Isolates CHN-BZJ2-2015 and CHN-WF224-2016 induced foot pad lesions and typical infectious synovitis, especially CHN-BZJ2-2015 group. Serologic response and tracheal mucosal thickness measurements at 21 days post infection indicated that CHN-BZJ2-2015 isolate was significantly more virulent than the CHN-JNB19-2016 isolate and MS-H strain(P<0.05). [Conclusion] The variation in virulence of MS isolates emphasizes the importance of active, on-going control and prevention of MS in the chicken flocks of China and accumulates experimental data for vaccine development.
引文
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[2]May M,Kleven SH,Brown DR.Sialidase activity in Mycoplasma synoviae[J].Avian Diseases,2007,51(4):829-833
[3]Sid H,Hartmann S,Petersen H,et al.Mycoplasma gallisepticum modifies the pathogenesis of influenza A virus in the avian tracheal epithelium[J].International Journal of Medical Microbiology,2016,306(3):174-186
[4]Stipkovits L,Glavits R,Palfi V,et al.Pathologic lesions caused by coinfection of Mycoplasma gallisepticum and H3N8 low pathogenic avian influenza virus in chickens[J].Veterinary Pathology,2012,49(2):273-283
[5]Raviv Z,Ferguson-Noel N,Laibinis V,et al.Role of Mycoplasma synoviae in commercial layer Escherichia coli peritonitis syndrome[J].Avian Diseases,2007,51(3):685-690
[6]Anderson GC,Bletner JK,Munro DA,et al.Studies of infectious synovitis in chickens[J].American Journal of Veterinary Research,1956,17(65):747-754
[7]Jordan FTW.Avian mycoplasma and Pathogenicity-a review[J].Avian Pathology,1975,4(3):165-174
[8]Feberwee A,de Vries TS,Landman WJM.Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms[J].Avian Pathology,2008,37(6):629-633
[9]Landman WJM.Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum?A viewpoint from the Netherlands[J].Avian Pathology,2014,43(1):2-8
[10]Kreizinger Z,Grózner D,Sulyok KM,et al.Antibiotic susceptibility profiles of Mycoplasma synoviae strains originating from Central and Eastern Europe[J].BMC Veterinary Research,2017,13(1):342
[11]Sun SK,Lin X,Liu JM,et al.Phylogenetic and pathogenic analysis of Mycoplasma synoviae isolated from native chicken breeds in China[J].Poultry Science,2017,96(7):2057-2063
[12]El-Gazzar M,Ghanem M,McDonald K,et al.Development of Multilocus Sequence Typing(MLST)for Mycoplasma synoviae[J].Avian Diseases,2017,61(1):25-32
[13]Du?ani?D,Be?i?RL,Cizelj I,et al.Mycoplasma synoviae invades non-phagocytic chicken cells in vitro[J].Veterinary Microbiology,2009,138(1/2):114-119
[14]Ghaniei A.Molecular characterization of Mycoplasma synoviae isolated from broiler chickens of West Azarbaijan province by PCR of vlhA gene[J].Veterinary Research Forum,2016,7(3):197-202
[15]Razin S,Tully JG.Methods in Mycoplasmology[M].New York:Academic Press,1983:185-196
[16]Raviv Z,Kleven SH.The development of diagnostic real-time taqman PCRs for the four pathogenic avian mycoplasmas[J].Avian Diseases,2009,53(1):103-107
[17]Kleven SH,Fletcher OJ,Davis RB.Influence of strain of Mycoplasma synoviae and route of infection on development of synovitis or airsacculitis in broilers[J].Avian Diseases,1975,19(1):126-135
[18]Whithear KG.Control of avian mycoplasmoses by vaccination[J].Revue Scientifique et Technique,1996,15(4):1527-1553
[19]Xue J,Xu MY,Ma ZJ,et al.Serological investigation of Mycoplasma synoviae infection in China from 2010 to 2015[J].Poultry Science,2017,96(9):3109-3112
[20]Dijkman R,Feberwee A,Landman WJM.Development,validation and field evaluation of a quantitative real-time PCRable to differentiate between field Mycoplasma synoviae and the MS-H-live vaccine strain[J].Avian Pathology,2017,46(4):403-415
[21]Feberwee A,Morrow CJ,Ghorashi SA,et al.Effect of a live Mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a M.synoviae infection preceded by an infection with infectious bronchitis virus D1466[J].Avian Pathology,2009,38(5):333-340
[22]Feberwee A,Dijkman R,Klinkenberg D,et al.Quantification of the horizontal transmission of Mycoplasma synoviae in non-vaccinated and MS-H-vaccinated layers[J].Avian Pathology,2017,46(4):346-358
[23]Kleven SH,King DD,Anderson DP.Airsacculitis in broilers from Mycoplasma synoviae:effect on air-sac lesions of vaccinating with infectious bronchitis and Newcastle virus[J].Avian Diseases,1972,16(4):915-924
[24]Ewing ML,Cookson KC,Phillips RA,et al.Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens[J].Avian Diseases,1998,42(2):230-238
[25]Noormohammadi AH,Jones JF,Harrigan KE,et al.Evaluation of the non-temperature-sensitive field clonal isolates of the Mycoplasma synoviae vaccine strain MS-H[J].Avian Diseases,2003,47(2):355-360
[26]Morrow CJ,Markham JF,Whithear KG.Production of temperature-sensitive clones of Mycoplasma synoviae for evaluation as live vaccines[J].Avian Diseases,1998,42(4):667-670
[2]May M,Kleven SH,Brown DR.Sialidase activity in Mycoplasma synoviae[J].Avian Diseases,2007,51(4):829-833
[3]Sid H,Hartmann S,Petersen H,et al.Mycoplasma gallisepticum modifies the pathogenesis of influenza A virus in the avian tracheal epithelium[J].International Journal of Medical Microbiology,2016,306(3):174-186
[4]Stipkovits L,Glavits R,Palfi V,et al.Pathologic lesions caused by coinfection of Mycoplasma gallisepticum and H3N8 low pathogenic avian influenza virus in chickens[J].Veterinary Pathology,2012,49(2):273-283
[5]Raviv Z,Ferguson-Noel N,Laibinis V,et al.Role of Mycoplasma synoviae in commercial layer Escherichia coli peritonitis syndrome[J].Avian Diseases,2007,51(3):685-690
[6]Anderson GC,Bletner JK,Munro DA,et al.Studies of infectious synovitis in chickens[J].American Journal of Veterinary Research,1956,17(65):747-754
[7]Jordan FTW.Avian mycoplasma and Pathogenicity-a review[J].Avian Pathology,1975,4(3):165-174
[8]Feberwee A,de Vries TS,Landman WJM.Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms[J].Avian Pathology,2008,37(6):629-633
[9]Landman WJM.Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum?A viewpoint from the Netherlands[J].Avian Pathology,2014,43(1):2-8
[10]Kreizinger Z,Grózner D,Sulyok KM,et al.Antibiotic susceptibility profiles of Mycoplasma synoviae strains originating from Central and Eastern Europe[J].BMC Veterinary Research,2017,13(1):342
[11]Sun SK,Lin X,Liu JM,et al.Phylogenetic and pathogenic analysis of Mycoplasma synoviae isolated from native chicken breeds in China[J].Poultry Science,2017,96(7):2057-2063
[12]El-Gazzar M,Ghanem M,McDonald K,et al.Development of Multilocus Sequence Typing(MLST)for Mycoplasma synoviae[J].Avian Diseases,2017,61(1):25-32
[13]Du?ani?D,Be?i?RL,Cizelj I,et al.Mycoplasma synoviae invades non-phagocytic chicken cells in vitro[J].Veterinary Microbiology,2009,138(1/2):114-119
[14]Ghaniei A.Molecular characterization of Mycoplasma synoviae isolated from broiler chickens of West Azarbaijan province by PCR of vlhA gene[J].Veterinary Research Forum,2016,7(3):197-202
[15]Razin S,Tully JG.Methods in Mycoplasmology[M].New York:Academic Press,1983:185-196
[16]Raviv Z,Kleven SH.The development of diagnostic real-time taqman PCRs for the four pathogenic avian mycoplasmas[J].Avian Diseases,2009,53(1):103-107
[17]Kleven SH,Fletcher OJ,Davis RB.Influence of strain of Mycoplasma synoviae and route of infection on development of synovitis or airsacculitis in broilers[J].Avian Diseases,1975,19(1):126-135
[18]Whithear KG.Control of avian mycoplasmoses by vaccination[J].Revue Scientifique et Technique,1996,15(4):1527-1553
[19]Xue J,Xu MY,Ma ZJ,et al.Serological investigation of Mycoplasma synoviae infection in China from 2010 to 2015[J].Poultry Science,2017,96(9):3109-3112
[20]Dijkman R,Feberwee A,Landman WJM.Development,validation and field evaluation of a quantitative real-time PCRable to differentiate between field Mycoplasma synoviae and the MS-H-live vaccine strain[J].Avian Pathology,2017,46(4):403-415
[21]Feberwee A,Morrow CJ,Ghorashi SA,et al.Effect of a live Mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a M.synoviae infection preceded by an infection with infectious bronchitis virus D1466[J].Avian Pathology,2009,38(5):333-340
[22]Feberwee A,Dijkman R,Klinkenberg D,et al.Quantification of the horizontal transmission of Mycoplasma synoviae in non-vaccinated and MS-H-vaccinated layers[J].Avian Pathology,2017,46(4):346-358
[23]Kleven SH,King DD,Anderson DP.Airsacculitis in broilers from Mycoplasma synoviae:effect on air-sac lesions of vaccinating with infectious bronchitis and Newcastle virus[J].Avian Diseases,1972,16(4):915-924
[24]Ewing ML,Cookson KC,Phillips RA,et al.Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens[J].Avian Diseases,1998,42(2):230-238
[25]Noormohammadi AH,Jones JF,Harrigan KE,et al.Evaluation of the non-temperature-sensitive field clonal isolates of the Mycoplasma synoviae vaccine strain MS-H[J].Avian Diseases,2003,47(2):355-360
[26]Morrow CJ,Markham JF,Whithear KG.Production of temperature-sensitive clones of Mycoplasma synoviae for evaluation as live vaccines[J].Avian Diseases,1998,42(4):667-670