去花对牛大力生物量和有效成分含量的影响
|
摘要
【目的】研究去花对牛大力生物量和药用部位块根有效成分含量的影响,为生产上疏花、疏荚果对牛大力块根的发育调控提供理论指导。【方法】以1年生热选1号牛大力苗为试验材料,根据"库—源"调控原理在花期进行去花处理,测定处理后70 d植株的叶片数及处理后7个月地上部和地下部的鲜干重、根条数、根长和根粗等生长指标及生物量,并采用高效液相色谱法(HPLC)测定牛大力块根中芒柄花素、高丽槐素和刺桐碱含量。【结果】与不去花对照相比,去花处理可极显著增加牛大力的叶片数、地下部鲜重和根粗(P<0.01,下同),同时地下部干重、根长和侧根数也显著增加(P<0.05,下同),各指标分别增加40.68%、72.56%、31.71%、105.35%、33.59%和49.25%,但根条数却显著减少37.74%。去花处理后牛大力块根中的有效成分刺桐碱含量极显著降低25.45%,芒柄花素含量显著降低40.42%。【结论】在牛大力栽培前期(1年生),去花处理可通过增加叶片数(增大光合面积)、根长度、粗度和侧根数进而增加产量,但有效成分含量明显降低。
【Objective】The effects of defloration on biomass and contents of active components of Millettia speciosa Champ. were studied. This would provide theoretical guidance for studying mechanism of root development by defloration and legume removing in practical production.【Method】The one-year-old M. speciosa Rexuanyihao seedlings were applied with defloration during flowering stage based on sink-source relation. The leaf number(70 d after defloration),shoot fresh and dry weight,root fresh and dry weight,root number,root length and root diameter(seven months after defloration)were measured. Then the contents of fermononetin,maackiain and erythrine in root were determined by high performance liquid chromatography(HPLC).【Rusult】The results showed that leaf number,root fresh weight,root diameter(P<0.01,the same below)extremely increased by 40.68%,72.56% and 31.71%,and underground dry weight,root length,lateral root number(P<0.05,the same below)significantly increased by 105.35%,33.59% and 49.25%, respectively compared with non-defloration,but the root number significantly decreased by 37.74% in the defloration treatment. The contents of fermononetin extremely decreased by 25.45%,and erythrine significantly decreased by 40.42% compared with the control.【Conclusion】Therefore,the defloration can increase yields by increasing leaf number(enlarge photosynthetic area),root length,root diameter and lateral root number at early growth stage of M. speciosa(one-year-old),but the active components contents reduce largely.
【Objective】The effects of defloration on biomass and contents of active components of Millettia speciosa Champ. were studied. This would provide theoretical guidance for studying mechanism of root development by defloration and legume removing in practical production.【Method】The one-year-old M. speciosa Rexuanyihao seedlings were applied with defloration during flowering stage based on sink-source relation. The leaf number(70 d after defloration),shoot fresh and dry weight,root fresh and dry weight,root number,root length and root diameter(seven months after defloration)were measured. Then the contents of fermononetin,maackiain and erythrine in root were determined by high performance liquid chromatography(HPLC).【Rusult】The results showed that leaf number,root fresh weight,root diameter(P<0.01,the same below)extremely increased by 40.68%,72.56% and 31.71%,and underground dry weight,root length,lateral root number(P<0.05,the same below)significantly increased by 105.35%,33.59% and 49.25%, respectively compared with non-defloration,but the root number significantly decreased by 37.74% in the defloration treatment. The contents of fermononetin extremely decreased by 25.45%,and erythrine significantly decreased by 40.42% compared with the control.【Conclusion】Therefore,the defloration can increase yields by increasing leaf number(enlarge photosynthetic area),root length,root diameter and lateral root number at early growth stage of M. speciosa(one-year-old),but the active components contents reduce largely.
引文
陈斌龙,张晓明.2011.留花蕾数对白术生长的影响[J].浙江农业科学,(3):539-540.[Chen B L,Zhang X M.2011.Effects of flower bud number on the growth of Atractylodes macrocephala[J].Zhejiang Agricultural Sciences,(3):539-540.]
陈晨.2016.海南牛大力植物成分分析及其质量控制研究[D].海口:海南大学.[Chen C.2016.The study of the components and quality control of Millettia speciosa in Hainan Province[D].Haikou:Hainan University.]
陈琪,陈兴福,杨文钰,张玉,王琦,赵一帆.2013.生殖生长对川泽泻源库关系的影响[J].中草药,44(12):1654-1660.[Chen Q,Chen X F,Yang W Y,Zhang Y,Wang Q,Zhao Y F.2013.Effect of reproductive growth on source-sink relationship of Alisma plantago-aquatica[J].Chinese Traditional and Herbal Drugs,44(12):1654-1660.]
黄璐琦,陈美兰,肖培根.2004.中药材道地性研究的现代生物学基础及模式假说[J].中国中药杂志,29(6):494-496.[Huang L Q,Chen M L,Xiao P G.2004.Modern biological basis and model hypothesis for the genuine study of Chinese medicinal materials[J].China Journal of Chinese Materia Medica,29(6):494-496.]
李冬萍.2013.中药材牛大力离体培养与繁育的研究[D].南宁:广西大学.[Li D P.2013.Study on culture and propagation in vitro of Chinese traditional medicine Millettia speciosa Champ.[D].Nanning:Guangxi University.]
李辉.2014.菊芋去花对菊芋生长发育的影响以及对参与生长阶段调控基因NF-YB家族的克隆和表达分析[D].南京:南京农业大学.[Li H.2014.The effect of defloration on Jerusalem artichoke growth and cloning and expression pattern of HTNE-YB family[D].Nanjing:Nanjing Agriculture University.]
李克烈,李志英,王荣香,徐立.2018.3种牛大力种质资源的光合特性的比较[J].分子植物育种,16(13):4413-4417.[Li K L,Li Z Y,Wang R X,Xu L.2018.Photosynthetic characteristics comparison of germplasm resources of three Callerya speciosa accessions[J].Molecular Plant Breeding,16(13):4413-4417.]
李淑如,潘永存,黄竹英,利幼,邓乔华.2009.采摘花蕾对丹参产量和质量的影响研究[J].现代中药研究与实践,22(6):10-12.[Li S R,Pan Y C,Huang Z Y,Li Y,Deng QH.2009.Effect of defloration on the yield and quality of Salvia miltiorrhiza Bge.[J].Research and Practice of Chinese Medicines,22(6):10-12.]
蔺海明,邱黛玉,陈垣.2007.当归苗根直径大小对提前抽薹率及产量的影响[J].中草药,38(9):1386-1389.[Lin HM,Qiu D Y,Chen Y.2007.Effect of root diameter on early bolting rate and yield in seedling of Angelica sinensis[J].Chinese Traditional and Herbal Drugs,38(9):1386-1389.]
孟祥才,孙晖,杨舸,王喜军.2008.打顶对北柴胡产量和质量的影响[J].中药材,31(3):336-337.[Meng X C,Sun H,Yang G,Wang X J.2008.The effect of topping on yield and quality of Bupleurum chinensis[J].Journal of Chinese Medicinal Materials,31(3):336-337.]
孟祥才,杨国辉,于冬梅,孙晖,王喜军.2010.不同性别及去花对穿龙薯蓣中薯蓣皂苷元含量的影响[J].时珍国医国药,21(11):2808-2809.[Meng X C,Yang G H,Yu DM,Sun H,Wang X J.2010.Effects of different genders and defloration on the content of diosgenin in Dioscorea nipponica[J].Lishizhen Medicine and Material Medica Research,21(11):2808-2809.]
潘颖南,张向军,蒙平,庾韦花,陈少珍,唐军,黄素梅,邹瑜.2010.药用植物牛大力组织培养初探[J].广西农业科学,41(6):523-525.[Pan Y N,Zhang X J,Meng P,Yu W H,Chen S Z,Tang J,Huang S M,Zou Y.2010.Tissue culture of medicinal plant Millettia speciosa Champ.[J].Guangxi Agricultural Sciences,41(6):523-525.]
潘颖南,张向军,庾韦花,蒙平,邓海燕,李婷.2016.牛大力植株生长特性及化学去蕾研究[J].西南农业学报,29(3):678-682.[Pan Y N,Zhang X J,Yu W H,Meng P,Deng H Y,Li T.2016.Study on plant growth characteristics and chemical removal of flower bud of Millettia specisoa Champ.[J].Southwest China Journal of Agricultural Sciences,29(3):678-682.]
单丽燕,刘辉,回飞,马金星,高凯.2016.去花对菊芋数量学性状的影响[J].北方园艺,(11):68-70.[Shan L Y,Liu H,Hui F,Ma J X,Gao K.2016.Effect of deflorate on quantitive trait of Helianthus tuberosus L.[J].Journal of North Horticulture,(11):68-70.]
时群,韦大器,陈丽文,朱开昕.2007.牛大力茎段组织培养污染率控制方法的初步研究[J].广西中医学院学报,(3):63-65.[Shi Q,Wei D Q,Chen L W,Zhu K X.2007.Preliminary study on the control method of contamination rate in stem culture of Millettia specisoa Champ.[J].Journal of Guangxi Traditional Chinese Medical University,(3):63-65.]
唐专辉,罗秋莲,莫静,张雪凤,朱艺萍,李燕婧,李典鹏,黄江燕,罗轩.2017.牛大力多糖对小鼠常压耐缺氧的研究[J].广西大学学报(自然科学版),42(3):1203-1208.[Tang Z H,Luo Q L,Mo J,Zhang X F,Zhu Y P,Li Y J,Li D P,Huang J Y,Luo X.2017.Study on mice’s normobaric hypoxia tolerance of polysaccharides from Millettia spe-ciosa Champ.[J].Journal of Guangxi University,42(3):1203-1208.]
王春华,王英,王国才,轧霁,张晓琦,叶文才.2008.牛大力的化学成分研究[J].中草药,39(7):972-975.[Wang C H,Wang Y,Wang G C,Ya J,Zhang X Q,Ye W C.2008.Chemical constituents from roots of Millettia speciosa[J].Chinese Traditional and Herbal Drugs,39(7):972-975.]
王祝年,赖富丽,王茂媛,王建荣.2011.牛大力根的化学成分研究[J].热带作物学报,32(12):2378-2380.[Wang ZN,Lai F L,Wang M Y,Wang J R.2011.Chemical constituents of the roots of Millettia speciosa[J].Chinese Journal of Tropical Crops,32(12):2378-2380.]
王祝年,李志英,徐立,黄碧兰.2005.牛大力的组织培养和快速繁殖[J].植物生理学通讯,41(6):800.[Wang Z N,Li Z Y,Xu L,Huang B L.2005.Tissue culture and rapid propagation of Millettia speciosa Champ.[J].Plant Physiology Communications,41(6):800.]
谢婵,黄慧学,邓日健,刘华钢,梁秋云.2016.甜牛大力和苦牛大力对免疫抑制小鼠免疫调节作用的比较研究[J].西安交通大学学报(医学版),37(3):451-454.[Xie C,Huang H X,Deng R J,Liu H G,Liang Q Y.2016.Comparison of the immunoregulatory effects of Radix millettia speciosa and Radix millettia Championi on immunousuppressed mice[J].Journal of Xi’an Jiaotong Unibersity(Medical Sciences),37(3):451-454.]
叶春秀.2016.牛大力膨大根特征及其化学调控的生理学机制[D].南宁:广西大学.[Ye C X.2016.Study on the characters of swollen root and the physiological effects of chemical regulators of Callerya speciosa[D].Nanning:Guangxi University.]
叶春秀,蓝芳,黄荣韶,李良,张平刚,钟霞军.2016.植物生长调节剂对牛大力种子萌发及幼苗质量的影响[J].种子,35(6):93-95.[Ye C X,Lan F,Huang R S,Li L,Zhang P G,Zhong X J.2016.Effect of plant growth regulators on seed germination of Millettia speciosa Champ.[J].Seed,35(6):93-95.]
尹红芳,晋小军.2009.控制抽薹对宽叶羌活产量和品质的影响[J].甘肃农业大学学报,44(3):77-80.[Yin H F,Jin X J.2009.Effect of controlling bolting on yield and quality from root of Notopterygium forbesii[J].Journal of Gansu Agricultural University,44(3):77-80.]
于英,王秀全,刘霞,王琦,赵淑春.2003.摘花序、打顶对北柴胡生育性状及根部产量的影响[J].吉林农业大学学报,25(3):303-306.[Yu Y,Wang X Q,Liu X,Wang Q,Zhao S C.2003.Influence of inflorescence plucking and tip pruning on vegetative characters and root production of Bupleurum chinense[J].Journal of Jilin Agricultural University,25(3):303-306.]
翟勇进,黄浩,白隆华,姚绍娥.2016.蔓生和攀援灌木牛大力的品质研究[J].中药材,39(2):299-301.[Zhai Y J,Huang H,Bai L H,Yao S E.2016.Study on the quality of vines and climbing Millettia speciosa Champ.[J].Journal of Chinese Medicinal Materials,39(2):299-301.]
张锋,王建华,余松烈,陈雨海,董庆裕.2006.源库比改变对白首乌产量形成的影响[J].中国中药杂志,31(5):372-375.[Zhang F,Wang J H,Yu S L,Chen Y H,Dong Q Y.2006.Effect of source-sink ratio changing on yield formation of Cynanchum bungei[J].China Journal of Chinese Materia Medice,31(5):372-375.]
张红瑞,高致明.2007.控制生殖生长对丹参根有效成分含量的影响[J].安徽农业科学,35(22):6811-6812.[Zhang H R,Gao Z M.2007.Effect of controlling the procreation growth on effective ingredients content in the roots of Salvia miltiorrhiza Bge.[J].Journal of Anhui Agricultural Science,35(22):6811-6812.]
张彦玲.2006.桔梗(Platycodon grandiflorum)生长发育和打顶、追肥对其产量和总皂甙含量积累动态的影响[D].郑州:河南农业大学.[Zhang Y L.2006.The growth and development of Platycodon grandiflorum and effect on dynamic accumulation of yield and the content of total saponins by topping off and top dressing[D].Zhengzhou:Henan Agriculture University.]
正英平,孔祥义,张连学,索滨华,张平.1996.人参摘蕾对同化产物在植株体内及皂甙中分配的影响[J].特产研究,(4):16-18.[Zheng Y P,Kong X Y,Zhang L X,Suo BH,Zhang P.1996.Effects of defloration on distribution of photosynthetic product and saponins in ginseng[J].Special Wild Economic Animal and Plant Research,(4):16-18.]
钟霞军,涂冬萍,王柳萍.2015.牛大力的化学成分及药理作用研究进展[J].广西中医药大学学报,18(3):73-75.[Zhong X J,Tu D P,Wang L P.2015.Research progress on chemical composition and pharmacological action of Millettia speciosa Champ.[J].Journal of Guangxi University of Chinese Medicine,18(3):73-75.]
周洁,邹琳,刘伟,方磊,李佳,赵红艳,王晓.2014.人工打花对黄芩药材生物量和有效物质积累的影响[J].西北林学院学报,29(2):135-138.[Zhou J,Zou L,Liu W,Fang L,Li J,Zhao H Y,Wang X.2014.Effects of defloration on the accumulation of biomass and bioactive substances of Scutellaria baicalensis[J].Journal of Northwest Forestry University,29(2):135-138.]
朱飞,冯继承.2007.开花结果对桔梗产量和总皂苷含量影响的对比研究[J].黑龙江医药,20(5):421-422.[Zhu F,Feng J C.2007.Comparative on the study of the influence of blooming and fruiting on the output and total sapoin of Platyeodom garndiflorum(Jacq.)A.DC.[J].Heilongjiang Medicine Journal,20(5):421-422.]
祝丽香,王建华,孙印石,唐行涛,耿慧云.2010.桔梗开花后可溶性糖和淀粉分配特性的研究[J].园艺学报,37(2):319-324.[Zhu L X,Wang J H,Sun Y S,Tang X T,Geng H Y.2010.The soluble sugar and starch allocation characteristics after flowering developing in Platycodon grandiflorium[J].Acta Horticulturae Sinica,37(2):319-324.]
陈晨.2016.海南牛大力植物成分分析及其质量控制研究[D].海口:海南大学.[Chen C.2016.The study of the components and quality control of Millettia speciosa in Hainan Province[D].Haikou:Hainan University.]
陈琪,陈兴福,杨文钰,张玉,王琦,赵一帆.2013.生殖生长对川泽泻源库关系的影响[J].中草药,44(12):1654-1660.[Chen Q,Chen X F,Yang W Y,Zhang Y,Wang Q,Zhao Y F.2013.Effect of reproductive growth on source-sink relationship of Alisma plantago-aquatica[J].Chinese Traditional and Herbal Drugs,44(12):1654-1660.]
黄璐琦,陈美兰,肖培根.2004.中药材道地性研究的现代生物学基础及模式假说[J].中国中药杂志,29(6):494-496.[Huang L Q,Chen M L,Xiao P G.2004.Modern biological basis and model hypothesis for the genuine study of Chinese medicinal materials[J].China Journal of Chinese Materia Medica,29(6):494-496.]
李冬萍.2013.中药材牛大力离体培养与繁育的研究[D].南宁:广西大学.[Li D P.2013.Study on culture and propagation in vitro of Chinese traditional medicine Millettia speciosa Champ.[D].Nanning:Guangxi University.]
李辉.2014.菊芋去花对菊芋生长发育的影响以及对参与生长阶段调控基因NF-YB家族的克隆和表达分析[D].南京:南京农业大学.[Li H.2014.The effect of defloration on Jerusalem artichoke growth and cloning and expression pattern of HTNE-YB family[D].Nanjing:Nanjing Agriculture University.]
李克烈,李志英,王荣香,徐立.2018.3种牛大力种质资源的光合特性的比较[J].分子植物育种,16(13):4413-4417.[Li K L,Li Z Y,Wang R X,Xu L.2018.Photosynthetic characteristics comparison of germplasm resources of three Callerya speciosa accessions[J].Molecular Plant Breeding,16(13):4413-4417.]
李淑如,潘永存,黄竹英,利幼,邓乔华.2009.采摘花蕾对丹参产量和质量的影响研究[J].现代中药研究与实践,22(6):10-12.[Li S R,Pan Y C,Huang Z Y,Li Y,Deng QH.2009.Effect of defloration on the yield and quality of Salvia miltiorrhiza Bge.[J].Research and Practice of Chinese Medicines,22(6):10-12.]
蔺海明,邱黛玉,陈垣.2007.当归苗根直径大小对提前抽薹率及产量的影响[J].中草药,38(9):1386-1389.[Lin HM,Qiu D Y,Chen Y.2007.Effect of root diameter on early bolting rate and yield in seedling of Angelica sinensis[J].Chinese Traditional and Herbal Drugs,38(9):1386-1389.]
孟祥才,孙晖,杨舸,王喜军.2008.打顶对北柴胡产量和质量的影响[J].中药材,31(3):336-337.[Meng X C,Sun H,Yang G,Wang X J.2008.The effect of topping on yield and quality of Bupleurum chinensis[J].Journal of Chinese Medicinal Materials,31(3):336-337.]
孟祥才,杨国辉,于冬梅,孙晖,王喜军.2010.不同性别及去花对穿龙薯蓣中薯蓣皂苷元含量的影响[J].时珍国医国药,21(11):2808-2809.[Meng X C,Yang G H,Yu DM,Sun H,Wang X J.2010.Effects of different genders and defloration on the content of diosgenin in Dioscorea nipponica[J].Lishizhen Medicine and Material Medica Research,21(11):2808-2809.]
潘颖南,张向军,蒙平,庾韦花,陈少珍,唐军,黄素梅,邹瑜.2010.药用植物牛大力组织培养初探[J].广西农业科学,41(6):523-525.[Pan Y N,Zhang X J,Meng P,Yu W H,Chen S Z,Tang J,Huang S M,Zou Y.2010.Tissue culture of medicinal plant Millettia speciosa Champ.[J].Guangxi Agricultural Sciences,41(6):523-525.]
潘颖南,张向军,庾韦花,蒙平,邓海燕,李婷.2016.牛大力植株生长特性及化学去蕾研究[J].西南农业学报,29(3):678-682.[Pan Y N,Zhang X J,Yu W H,Meng P,Deng H Y,Li T.2016.Study on plant growth characteristics and chemical removal of flower bud of Millettia specisoa Champ.[J].Southwest China Journal of Agricultural Sciences,29(3):678-682.]
单丽燕,刘辉,回飞,马金星,高凯.2016.去花对菊芋数量学性状的影响[J].北方园艺,(11):68-70.[Shan L Y,Liu H,Hui F,Ma J X,Gao K.2016.Effect of deflorate on quantitive trait of Helianthus tuberosus L.[J].Journal of North Horticulture,(11):68-70.]
时群,韦大器,陈丽文,朱开昕.2007.牛大力茎段组织培养污染率控制方法的初步研究[J].广西中医学院学报,(3):63-65.[Shi Q,Wei D Q,Chen L W,Zhu K X.2007.Preliminary study on the control method of contamination rate in stem culture of Millettia specisoa Champ.[J].Journal of Guangxi Traditional Chinese Medical University,(3):63-65.]
唐专辉,罗秋莲,莫静,张雪凤,朱艺萍,李燕婧,李典鹏,黄江燕,罗轩.2017.牛大力多糖对小鼠常压耐缺氧的研究[J].广西大学学报(自然科学版),42(3):1203-1208.[Tang Z H,Luo Q L,Mo J,Zhang X F,Zhu Y P,Li Y J,Li D P,Huang J Y,Luo X.2017.Study on mice’s normobaric hypoxia tolerance of polysaccharides from Millettia spe-ciosa Champ.[J].Journal of Guangxi University,42(3):1203-1208.]
王春华,王英,王国才,轧霁,张晓琦,叶文才.2008.牛大力的化学成分研究[J].中草药,39(7):972-975.[Wang C H,Wang Y,Wang G C,Ya J,Zhang X Q,Ye W C.2008.Chemical constituents from roots of Millettia speciosa[J].Chinese Traditional and Herbal Drugs,39(7):972-975.]
王祝年,赖富丽,王茂媛,王建荣.2011.牛大力根的化学成分研究[J].热带作物学报,32(12):2378-2380.[Wang ZN,Lai F L,Wang M Y,Wang J R.2011.Chemical constituents of the roots of Millettia speciosa[J].Chinese Journal of Tropical Crops,32(12):2378-2380.]
王祝年,李志英,徐立,黄碧兰.2005.牛大力的组织培养和快速繁殖[J].植物生理学通讯,41(6):800.[Wang Z N,Li Z Y,Xu L,Huang B L.2005.Tissue culture and rapid propagation of Millettia speciosa Champ.[J].Plant Physiology Communications,41(6):800.]
谢婵,黄慧学,邓日健,刘华钢,梁秋云.2016.甜牛大力和苦牛大力对免疫抑制小鼠免疫调节作用的比较研究[J].西安交通大学学报(医学版),37(3):451-454.[Xie C,Huang H X,Deng R J,Liu H G,Liang Q Y.2016.Comparison of the immunoregulatory effects of Radix millettia speciosa and Radix millettia Championi on immunousuppressed mice[J].Journal of Xi’an Jiaotong Unibersity(Medical Sciences),37(3):451-454.]
叶春秀.2016.牛大力膨大根特征及其化学调控的生理学机制[D].南宁:广西大学.[Ye C X.2016.Study on the characters of swollen root and the physiological effects of chemical regulators of Callerya speciosa[D].Nanning:Guangxi University.]
叶春秀,蓝芳,黄荣韶,李良,张平刚,钟霞军.2016.植物生长调节剂对牛大力种子萌发及幼苗质量的影响[J].种子,35(6):93-95.[Ye C X,Lan F,Huang R S,Li L,Zhang P G,Zhong X J.2016.Effect of plant growth regulators on seed germination of Millettia speciosa Champ.[J].Seed,35(6):93-95.]
尹红芳,晋小军.2009.控制抽薹对宽叶羌活产量和品质的影响[J].甘肃农业大学学报,44(3):77-80.[Yin H F,Jin X J.2009.Effect of controlling bolting on yield and quality from root of Notopterygium forbesii[J].Journal of Gansu Agricultural University,44(3):77-80.]
于英,王秀全,刘霞,王琦,赵淑春.2003.摘花序、打顶对北柴胡生育性状及根部产量的影响[J].吉林农业大学学报,25(3):303-306.[Yu Y,Wang X Q,Liu X,Wang Q,Zhao S C.2003.Influence of inflorescence plucking and tip pruning on vegetative characters and root production of Bupleurum chinense[J].Journal of Jilin Agricultural University,25(3):303-306.]
翟勇进,黄浩,白隆华,姚绍娥.2016.蔓生和攀援灌木牛大力的品质研究[J].中药材,39(2):299-301.[Zhai Y J,Huang H,Bai L H,Yao S E.2016.Study on the quality of vines and climbing Millettia speciosa Champ.[J].Journal of Chinese Medicinal Materials,39(2):299-301.]
张锋,王建华,余松烈,陈雨海,董庆裕.2006.源库比改变对白首乌产量形成的影响[J].中国中药杂志,31(5):372-375.[Zhang F,Wang J H,Yu S L,Chen Y H,Dong Q Y.2006.Effect of source-sink ratio changing on yield formation of Cynanchum bungei[J].China Journal of Chinese Materia Medice,31(5):372-375.]
张红瑞,高致明.2007.控制生殖生长对丹参根有效成分含量的影响[J].安徽农业科学,35(22):6811-6812.[Zhang H R,Gao Z M.2007.Effect of controlling the procreation growth on effective ingredients content in the roots of Salvia miltiorrhiza Bge.[J].Journal of Anhui Agricultural Science,35(22):6811-6812.]
张彦玲.2006.桔梗(Platycodon grandiflorum)生长发育和打顶、追肥对其产量和总皂甙含量积累动态的影响[D].郑州:河南农业大学.[Zhang Y L.2006.The growth and development of Platycodon grandiflorum and effect on dynamic accumulation of yield and the content of total saponins by topping off and top dressing[D].Zhengzhou:Henan Agriculture University.]
正英平,孔祥义,张连学,索滨华,张平.1996.人参摘蕾对同化产物在植株体内及皂甙中分配的影响[J].特产研究,(4):16-18.[Zheng Y P,Kong X Y,Zhang L X,Suo BH,Zhang P.1996.Effects of defloration on distribution of photosynthetic product and saponins in ginseng[J].Special Wild Economic Animal and Plant Research,(4):16-18.]
钟霞军,涂冬萍,王柳萍.2015.牛大力的化学成分及药理作用研究进展[J].广西中医药大学学报,18(3):73-75.[Zhong X J,Tu D P,Wang L P.2015.Research progress on chemical composition and pharmacological action of Millettia speciosa Champ.[J].Journal of Guangxi University of Chinese Medicine,18(3):73-75.]
周洁,邹琳,刘伟,方磊,李佳,赵红艳,王晓.2014.人工打花对黄芩药材生物量和有效物质积累的影响[J].西北林学院学报,29(2):135-138.[Zhou J,Zou L,Liu W,Fang L,Li J,Zhao H Y,Wang X.2014.Effects of defloration on the accumulation of biomass and bioactive substances of Scutellaria baicalensis[J].Journal of Northwest Forestry University,29(2):135-138.]
朱飞,冯继承.2007.开花结果对桔梗产量和总皂苷含量影响的对比研究[J].黑龙江医药,20(5):421-422.[Zhu F,Feng J C.2007.Comparative on the study of the influence of blooming and fruiting on the output and total sapoin of Platyeodom garndiflorum(Jacq.)A.DC.[J].Heilongjiang Medicine Journal,20(5):421-422.]
祝丽香,王建华,孙印石,唐行涛,耿慧云.2010.桔梗开花后可溶性糖和淀粉分配特性的研究[J].园艺学报,37(2):319-324.[Zhu L X,Wang J H,Sun Y S,Tang X T,Geng H Y.2010.The soluble sugar and starch allocation characteristics after flowering developing in Platycodon grandiflorium[J].Acta Horticulturae Sinica,37(2):319-324.]