TLR-8在慢性伴鼻息肉中的表达
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摘要
目的通过检测鼻黏膜上皮组织中Toll样受体(TLR)-8的表达,揭示慢性鼻-鼻窦炎伴鼻息肉的发病机制。方法应用免疫组织化学方法检测18例鼻中隔偏曲伴下鼻甲肥大患者下鼻甲鼻黏膜上皮组织(对照组)和50例慢性鼻-鼻窦炎伴鼻息肉(鼻息肉组)黏膜上皮组织中TLR-8的表达。应用SPSS13.0软件对鼻息肉组及对照组相关指标进行统计学分析。结果 TLR-8在鼻息肉组中的平均光密度值为0.52±0.06,而其在对照组中为0.30±0.05,差异有统计学意义(P<0.05)。鼻息肉组和对照组中TLR-8蛋白的阳性表达率分别为76.0%和27.8%,差异有统计学意义(P<0.05)。结论 TLR-8可能参与了鼻黏膜上皮细胞免疫防御性炎性反应。
Objective To reveal the pathogenesis of chronic rhinosinusitis complicating nasal polyps by detecting the expression of Toll-like receptor-8(TLR-8)in nasal mucosa epithelial tissue.Methods The expression of TLR-8 in inferior turbinate mucosal tissue of 18 patients with nasal septum deviation complicating inferior turbinate hypertrophy(control group)and in mucosal epithelial tissue of 50 patients with chronic rhinosinusitis complicating nasal polyps(nosal polyps group)were detected by using the immunohistochemistry method.The SPSS13.0 software was used to conduct the statistical analysis on the related indicators in the nasal polyps group and control group.Results The average integral optical density value of TLR-8 in the nasal polyps group was 0.52±0.06,which in the control group was 0.30±0.05,and the difference was statistically significant(P<0.05).The positive expression rates of TLR-8 in the nasal polyps group and the control group were 76.0%and 27.8%,respectively,and the difference was statistically significant(P<0.05).Conclusion TLR-8 might participate in immune defensive response of nasal mucosal epithelial cells.
Objective To reveal the pathogenesis of chronic rhinosinusitis complicating nasal polyps by detecting the expression of Toll-like receptor-8(TLR-8)in nasal mucosa epithelial tissue.Methods The expression of TLR-8 in inferior turbinate mucosal tissue of 18 patients with nasal septum deviation complicating inferior turbinate hypertrophy(control group)and in mucosal epithelial tissue of 50 patients with chronic rhinosinusitis complicating nasal polyps(nosal polyps group)were detected by using the immunohistochemistry method.The SPSS13.0 software was used to conduct the statistical analysis on the related indicators in the nasal polyps group and control group.Results The average integral optical density value of TLR-8 in the nasal polyps group was 0.52±0.06,which in the control group was 0.30±0.05,and the difference was statistically significant(P<0.05).The positive expression rates of TLR-8 in the nasal polyps group and the control group were 76.0%and 27.8%,respectively,and the difference was statistically significant(P<0.05).Conclusion TLR-8 might participate in immune defensive response of nasal mucosal epithelial cells.
引文
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[14]MELVIN T A,LANE A P,NGUYEN M T,et al.Sinonasal epithelial cell expression of Toll-like receptor 9is elevated in cystic fibrosis-associated chronic rhinosinusitis[J].Am J Rhinol Allergy,2013,27(1):30-33.
[15]顾兆伟,曹志伟,王韫秀.TLR2和TLR4在慢性鼻窦炎鼻息肉中的表达及临床意义[J].中国耳鼻咽喉颅底外科杂志,2013,19(1):43-47.
[16]ZHAO C Y,WANG X,LIU M,et al.Microarray gene analysis of Toll-like receptor signaling elements in chronic rhinosinusitis with nasal polyps[J].Int Arch Allergy Immunol,2011,156(3):297-304.
[17]汪际云,黄春鑫,乐东海,等.鼻黏膜中Toll样受体5的表达[J].中国耳鼻咽喉头颈外科,2012,19(6):315-318.
[18]ZHANG Q,WANG C H,HAN D M,et al.Differential expression of Toll-like receptor pathway genes in chronic rhinosinusitis with or without nasal polyps[J].Acta OtoLaryngol,2013,133(2):165-173.
[2]KUMAGAI Y,AKIRA S.Identification and functions of pattern-recognition receptors[J].J Allergy Clin Immunol,2010,125(5):985-992.
[3]BASITH S,MANAVALAN B,YOO T H,et al.Roles of toll-like receptors in cancer:a double-edged sword for defense and offense[J].Arch Pharm Res,2012,35(8):1297-1316.
[4]VANDERMEER J,SHA Q,LANE A P,et al.Innate immunity of the sinonasal cavity:expression of messenger RNA for complement cascade components and toll-like receptors[J].Arch Otolaryngol Head Neck Surg,2004,130(12):1374-1380.
[5]THOMPSON M R,KAMINSKI J J,KURT-JONES E A.Pattern recognition receptors and the innate immune response to viral infection[J].Viruses,2011,3(6):920-940.
[6]LUND J M,ALEXOPOULOU L,SATO A,et al.Recognition of single-stranded RNA viruses by Toll-like receptor 7[J].Proc Natl Acad Sci U S A,2004,101(15):5598-5603.
[7]RIFKIN I R,LEADBETTER E A,BUSCONI L,et al.Toll-like receptors,endogenous ligands,and systemic autoimmune disease[J].Immunol Rev,2005,204(1):27-42.
[8]黄朝平,张建辉,朱力,等.紧密连接蛋白Claudin-1与变应性鼻炎发病机制相关性初探[J].中国眼耳鼻喉科杂志,2014,14(5):283-286.
[9]刘锐越,刘涛,周华容.TLR5在变应性鼻炎患者鼻黏膜组织中的表达[J].中国医药科学,2014,4(3):58-62.
[10]李杰恩,唐臻臻,王扬,等.改良切口的鼻中隔成形术疗效观察[J].中国耳鼻咽喉烦底外科杂志,2016,20(1):38-42.
[11]LICHTENEGGER F S,MUELLER K,OTTE B,et al.CD86and IL-12p70are key players for T helper 1polarization and natural killer cell activation by Toll-like receptor-induced dendritic cells[J].PLoS One,2012,7(9):e44266.
[12]LAURIELLO M,MICERA A,MUZI PAOLA,et al.TLR4and TLR9expression in different phenotypes of rhinitis[J].Int J Otolaryngol,2012,2012(1):1-7.
[13]夏忠芳,王智楠,乐建新,等.TLR9在儿童慢性鼻-鼻窦炎及正常鼻黏膜组织中的差异表达[J].华中科技大学学报(医学版),2013,42(5):582-584.
[14]MELVIN T A,LANE A P,NGUYEN M T,et al.Sinonasal epithelial cell expression of Toll-like receptor 9is elevated in cystic fibrosis-associated chronic rhinosinusitis[J].Am J Rhinol Allergy,2013,27(1):30-33.
[15]顾兆伟,曹志伟,王韫秀.TLR2和TLR4在慢性鼻窦炎鼻息肉中的表达及临床意义[J].中国耳鼻咽喉颅底外科杂志,2013,19(1):43-47.
[16]ZHAO C Y,WANG X,LIU M,et al.Microarray gene analysis of Toll-like receptor signaling elements in chronic rhinosinusitis with nasal polyps[J].Int Arch Allergy Immunol,2011,156(3):297-304.
[17]汪际云,黄春鑫,乐东海,等.鼻黏膜中Toll样受体5的表达[J].中国耳鼻咽喉头颈外科,2012,19(6):315-318.
[18]ZHANG Q,WANG C H,HAN D M,et al.Differential expression of Toll-like receptor pathway genes in chronic rhinosinusitis with or without nasal polyps[J].Acta OtoLaryngol,2013,133(2):165-173.