蝉虫草子实体与发酵菌丝体胞内多糖成分分析
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摘要
比较蝉虫草子实体、孢梗束、虫体以及蝉虫草发酵菌丝体的胞内多糖含量、单糖组成和分子量差异,结果表明,蝉虫草子实体与发酵菌丝中胞内多糖的含量和组成均存在差异。葡萄糖、半乳糖和甘露糖是蝉虫草发酵菌丝体和子实体粗多糖中主要的3种单糖,阿拉伯糖和木糖是子实体粗多糖中的特征性单糖,而果糖属于菌丝体的特征性单糖。子实体粗多糖中高分子量组分(>1×106Da)的相对含量明显高于菌丝体粗多糖。本研究对提升蝉虫草发酵多糖产品品质、促进蝉虫草开发应用具有参考价值。
The content, monosaccharide composition, and molecular weight distribution of the intracellular polysaccharides extracted from the fruiting bodies and fermented mycelia of Cordyceps cicadae were compared. The results showed that the composition and structure of the crude polysaccharides from C.cicadae fruiting bodies and mycelia were different. Glucose, galactose, and mannose were the main monosaccharides in fruiting bodies and mycelia. Arabinose and xylose were found to only exist in the polysaccharides from fruiting bodies, while fructose was only present in mycelia. Relative content of high molecular weight polysaccharides in the fruiting bodies(>1×106 Da) was obviously higher than that in mycelia.The results of this study may benefit the development and quality improvement of the polysaccharide product of the fermented C. cicadae.
The content, monosaccharide composition, and molecular weight distribution of the intracellular polysaccharides extracted from the fruiting bodies and fermented mycelia of Cordyceps cicadae were compared. The results showed that the composition and structure of the crude polysaccharides from C.cicadae fruiting bodies and mycelia were different. Glucose, galactose, and mannose were the main monosaccharides in fruiting bodies and mycelia. Arabinose and xylose were found to only exist in the polysaccharides from fruiting bodies, while fructose was only present in mycelia. Relative content of high molecular weight polysaccharides in the fruiting bodies(>1×106 Da) was obviously higher than that in mycelia.The results of this study may benefit the development and quality improvement of the polysaccharide product of the fermented C. cicadae.
引文
Cheng JW,Wang YB,He L,Qian H,Fu LZ,Li HB,2012.Optimization of fermentation process for the production of intracellular polysaccharide from Paecilomyces cicadae and the immuno-stimulating activity of intracellular polysaccharide.World Journal of Microbiology and Biotechnology,28(12):3293-3299
Dong CH,Li WJ,Li ZZ,Yan WJ,Li TH,Liu XZ,Cai L,Zeng WB,Chai MQ,Chen SJ,Chen ZH,Dai YD,Dai TJ,Du XF,Gu YY,Guo LD,Guo XL,Guo YL,Han RC,He PP,He ZY,Huang XZ,Ke CK,Kong DY,Lang N,Li B,Li Y,Li CR,Li JG,Li PR,Li YL,Lin WF,Liu WH,Liu YF,Liu YQ,Liu ZY,Lu ZM,Luo XC,Lv ZZ,Ma X,Qian ZM,Shi XQ,Shi JS,Wang Y,Wang CS,Wang GQ,Wang WF,Wang YB,Wang M,Wen L,Wei JC,Wen G,Wu GS,Xiao B,Xie F,Xin SQ,Xu J,Xu GH,Xu HY,Xu TH,Xu ZH,Yang WX,Yi SF,Yu XJ,Yu H,Yuan F,Zhai LT,Zhao FL,Zhu JM,Zhu RC,2016.Cordyceps industry in China:current status,challenges and perspectives-Jinhu declaration for cordyceps industry development.Mycosystema,35(1):1-15(in Chinese)
Du Q,2011.Research advancement of edible(medicinal)fungi polysaccharides.Food and Nutrition in China,17(5):75-77(in Chinese)
Kiho T,Nagai K,Miyamoto I,Watanabe T,Ukai S,1990.Polysaccharides in fungi.XXV.Biological activities of two galactomannans from the insect-body portion of Chan hua(fungus:Cordyceps cicadae).Yakugaku zasshi:Journal of the Pharmaceutical Society of Japan,110(4):286-288
Kim HS,Kim YJ,Lee HK,Ryu HS,Kim JS,Yoon MJ,Kang JS,Hong JT,Kim Y,Han SB,2012.Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4.Food and Chemical Toxicology,50(9):3190-3197
Kuo YC,Weng SC,Chou CJ,Chang TT,Tsai WJ,2003.Activation and proliferation signals in primary human Tlymphocytes inhibited by ergosterol peroxide isolated from Cordyceps cicadae.British Journal of Pharmacology,140(5):895-906
Nie S,Zhang H,Li W,Xie M,2013.Current development of polysaccharides from Ganoderma:isolation,structure and bioactivities.Bioactive Carbohydrates and Dietary Fibre,1(1):10-20
Olatunji OJ,Feng Y,Olatunji OO,Tang J,Wei Y,Ouyang Z,Su Z,2016.Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage.Carbohydrate Polymers,153:187-195
Ren X,He L,Cheng J,Chang J,2014.Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae(Miquel)Samson and its antioxidant activities in vitro.PLoS One,9(2):e87578
Sharma SK,Gautam N,Atri NS,2015.Optimized extraction,composition,antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.BMC Complementary and Alternative Medicine,15:446
Wei CY,Li WQ,Shao SS,He L,Cheng J,Han S,Liu Y,2016.Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae.Carbohydrate Polymers,136:728-737
Wei YL,Yang MF,Zou X,Liu AY,2014.Review on bioactive components from Isaria cicadae.Guizhou Agricultural Sciences,2014(12):142-148(in Chinese)
Weng L,2010.Study on the extraction of polysaccharide from wild and artificial Cordyceps sobolifera and bacteriostasis effect.Agricultural Science and Technology and Equipment,2010(11):22-24+29(in Chinese)
Xie F,Li W,Chen MZ,Yu J,2016.Antitumor activity and mechanism of action of wild Cordyceps sobolifera polysaccharide.Food Science,37(13):209-213(in Chinese)
Xu HJ,Mo ZH,Yu JW,Mao XB,Zhu HL,2010.Separation and purification of anti-fungal compound from Cordyceps cicadae.Natural Product Research and Development,22(5):794-797+835(in Chinese)
Yang FQ,Guan J,Li SP,2007.Fast simultaneous determination of 14 nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography.Talanta,73(2):269-273
Yang JZ,Zhuo J,Chen BK,Jin LQ,LüJX,Li LJ,2008.Regulating effects of Paecilomyces cicadae polysaccharides on immunity of aged rats.China Journal of Chinese Materia Medica,33(3):292-295(in Chinese)
Yang S,Zhang H,2016.Optimization of the fermentation process of Cordyceps sobolifera Se-CEPS and its anti-tumor activity in vivo.Journal of Biological Engineering,10:8
Zeng WB,Yu H,Ge F,Yang JY,Chen ZH,Wang YB,Dai YD,Adams A,2014.Distribution of nucleosides in populations of Cordyceps cicadae.Molecules,19(5):6123-6141
Zhang HX,Gao XH,Chen W,Huang KH,2016.Comparison of active ingredients in Cordyceps sobolifera coremium cultivated artificially on different media.Acta Agriculturae Shanghai,2016(5):101-104(in Chinese)
Zhu R,Chen YP,Deng YY,Zheng R,Zhong YF,Wang L,Du LP,2011.Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model.Journal of Zhejiang University Science B,12(12):1024-1033
Zhu R,Zheng R,Deng Y,Chen Y,Zhang S,2014.Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta1-induced activation of kidney fibroblasts.Phytomedicine,21(3):372-378
董彩虹,李文佳,李增智,闫文娟,李泰辉,刘杏忠,蔡磊,曾文波,柴美清,陈仕江,陈自宏,代永东,戴天机,都兴范,顾寅钰,郭良栋,郭晓玲,郭英兰,韩日畴,何沛平,贺宗毅,黄显智,柯传奎,孔德勇,郎宁,李兵,李毅,李春如,李建刚,李培荣,李玉玲,林文飞,刘文海,刘艳芳,刘乙清,刘作易,陆震鸣,罗信昌,吕作舟,马欣,钱正明,施新琴,史劲松,王莹,王成树,王国强,王文风,王元兵,旺姆,温鲁,魏江春,文戈,吴根土,肖波,谢放,辛树权,徐娟,徐国华,徐鸿雁,徐泰浩,许正宏,阳武雄,易斯富,于兴军,虞泓,袁峰,翟立泰,赵丰丽,朱景明,朱荣才,2016.我国虫草产业发展现状、问题及展望.菌物学报,35(1):1-15
杜庆,2011.食(药)用真菌多糖的研究进展.中国食物与营养,17(5):75-77
卫亚丽,杨茂发,邹晓,刘爱英,2014.蝉棒束孢菌的生物学活性研究进展.贵州农业科学,2014(12):142-148
翁梁,2010.野生蝉花与人工蝉花多糖的提取及抑菌试验.农业科技与装备,2010(11):22-24+29
谢飞,李伟,陈美珍,余杰,2016.野生蝉花多糖抗肿瘤活性及其作用机制.食品科学,37(13):209-213
徐红娟,莫志宏,余佳文,毛先兵,朱华李,2010.蝉花抗真菌活性成分的分离纯化研究.天然产物研究与开发,22(5):794-797+835
杨介钻,卓佳,陈柏坤,金丽琴,吕建新,李兰娟,2008.蝉拟青霉多糖对老年大鼠免疫功能的调节作用.中国中药杂志,33(3):292-295
张红霞,高新华,陈伟,黄开华,2016.不同培养基人工培育蝉花中有效成分的比较.上海农业学报,2016(5):101-104
Dong CH,Li WJ,Li ZZ,Yan WJ,Li TH,Liu XZ,Cai L,Zeng WB,Chai MQ,Chen SJ,Chen ZH,Dai YD,Dai TJ,Du XF,Gu YY,Guo LD,Guo XL,Guo YL,Han RC,He PP,He ZY,Huang XZ,Ke CK,Kong DY,Lang N,Li B,Li Y,Li CR,Li JG,Li PR,Li YL,Lin WF,Liu WH,Liu YF,Liu YQ,Liu ZY,Lu ZM,Luo XC,Lv ZZ,Ma X,Qian ZM,Shi XQ,Shi JS,Wang Y,Wang CS,Wang GQ,Wang WF,Wang YB,Wang M,Wen L,Wei JC,Wen G,Wu GS,Xiao B,Xie F,Xin SQ,Xu J,Xu GH,Xu HY,Xu TH,Xu ZH,Yang WX,Yi SF,Yu XJ,Yu H,Yuan F,Zhai LT,Zhao FL,Zhu JM,Zhu RC,2016.Cordyceps industry in China:current status,challenges and perspectives-Jinhu declaration for cordyceps industry development.Mycosystema,35(1):1-15(in Chinese)
Du Q,2011.Research advancement of edible(medicinal)fungi polysaccharides.Food and Nutrition in China,17(5):75-77(in Chinese)
Kiho T,Nagai K,Miyamoto I,Watanabe T,Ukai S,1990.Polysaccharides in fungi.XXV.Biological activities of two galactomannans from the insect-body portion of Chan hua(fungus:Cordyceps cicadae).Yakugaku zasshi:Journal of the Pharmaceutical Society of Japan,110(4):286-288
Kim HS,Kim YJ,Lee HK,Ryu HS,Kim JS,Yoon MJ,Kang JS,Hong JT,Kim Y,Han SB,2012.Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4.Food and Chemical Toxicology,50(9):3190-3197
Kuo YC,Weng SC,Chou CJ,Chang TT,Tsai WJ,2003.Activation and proliferation signals in primary human Tlymphocytes inhibited by ergosterol peroxide isolated from Cordyceps cicadae.British Journal of Pharmacology,140(5):895-906
Nie S,Zhang H,Li W,Xie M,2013.Current development of polysaccharides from Ganoderma:isolation,structure and bioactivities.Bioactive Carbohydrates and Dietary Fibre,1(1):10-20
Olatunji OJ,Feng Y,Olatunji OO,Tang J,Wei Y,Ouyang Z,Su Z,2016.Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage.Carbohydrate Polymers,153:187-195
Ren X,He L,Cheng J,Chang J,2014.Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae(Miquel)Samson and its antioxidant activities in vitro.PLoS One,9(2):e87578
Sharma SK,Gautam N,Atri NS,2015.Optimized extraction,composition,antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.BMC Complementary and Alternative Medicine,15:446
Wei CY,Li WQ,Shao SS,He L,Cheng J,Han S,Liu Y,2016.Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae.Carbohydrate Polymers,136:728-737
Wei YL,Yang MF,Zou X,Liu AY,2014.Review on bioactive components from Isaria cicadae.Guizhou Agricultural Sciences,2014(12):142-148(in Chinese)
Weng L,2010.Study on the extraction of polysaccharide from wild and artificial Cordyceps sobolifera and bacteriostasis effect.Agricultural Science and Technology and Equipment,2010(11):22-24+29(in Chinese)
Xie F,Li W,Chen MZ,Yu J,2016.Antitumor activity and mechanism of action of wild Cordyceps sobolifera polysaccharide.Food Science,37(13):209-213(in Chinese)
Xu HJ,Mo ZH,Yu JW,Mao XB,Zhu HL,2010.Separation and purification of anti-fungal compound from Cordyceps cicadae.Natural Product Research and Development,22(5):794-797+835(in Chinese)
Yang FQ,Guan J,Li SP,2007.Fast simultaneous determination of 14 nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography.Talanta,73(2):269-273
Yang JZ,Zhuo J,Chen BK,Jin LQ,LüJX,Li LJ,2008.Regulating effects of Paecilomyces cicadae polysaccharides on immunity of aged rats.China Journal of Chinese Materia Medica,33(3):292-295(in Chinese)
Yang S,Zhang H,2016.Optimization of the fermentation process of Cordyceps sobolifera Se-CEPS and its anti-tumor activity in vivo.Journal of Biological Engineering,10:8
Zeng WB,Yu H,Ge F,Yang JY,Chen ZH,Wang YB,Dai YD,Adams A,2014.Distribution of nucleosides in populations of Cordyceps cicadae.Molecules,19(5):6123-6141
Zhang HX,Gao XH,Chen W,Huang KH,2016.Comparison of active ingredients in Cordyceps sobolifera coremium cultivated artificially on different media.Acta Agriculturae Shanghai,2016(5):101-104(in Chinese)
Zhu R,Chen YP,Deng YY,Zheng R,Zhong YF,Wang L,Du LP,2011.Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model.Journal of Zhejiang University Science B,12(12):1024-1033
Zhu R,Zheng R,Deng Y,Chen Y,Zhang S,2014.Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta1-induced activation of kidney fibroblasts.Phytomedicine,21(3):372-378
董彩虹,李文佳,李增智,闫文娟,李泰辉,刘杏忠,蔡磊,曾文波,柴美清,陈仕江,陈自宏,代永东,戴天机,都兴范,顾寅钰,郭良栋,郭晓玲,郭英兰,韩日畴,何沛平,贺宗毅,黄显智,柯传奎,孔德勇,郎宁,李兵,李毅,李春如,李建刚,李培荣,李玉玲,林文飞,刘文海,刘艳芳,刘乙清,刘作易,陆震鸣,罗信昌,吕作舟,马欣,钱正明,施新琴,史劲松,王莹,王成树,王国强,王文风,王元兵,旺姆,温鲁,魏江春,文戈,吴根土,肖波,谢放,辛树权,徐娟,徐国华,徐鸿雁,徐泰浩,许正宏,阳武雄,易斯富,于兴军,虞泓,袁峰,翟立泰,赵丰丽,朱景明,朱荣才,2016.我国虫草产业发展现状、问题及展望.菌物学报,35(1):1-15
杜庆,2011.食(药)用真菌多糖的研究进展.中国食物与营养,17(5):75-77
卫亚丽,杨茂发,邹晓,刘爱英,2014.蝉棒束孢菌的生物学活性研究进展.贵州农业科学,2014(12):142-148
翁梁,2010.野生蝉花与人工蝉花多糖的提取及抑菌试验.农业科技与装备,2010(11):22-24+29
谢飞,李伟,陈美珍,余杰,2016.野生蝉花多糖抗肿瘤活性及其作用机制.食品科学,37(13):209-213
徐红娟,莫志宏,余佳文,毛先兵,朱华李,2010.蝉花抗真菌活性成分的分离纯化研究.天然产物研究与开发,22(5):794-797+835
杨介钻,卓佳,陈柏坤,金丽琴,吕建新,李兰娟,2008.蝉拟青霉多糖对老年大鼠免疫功能的调节作用.中国中药杂志,33(3):292-295
张红霞,高新华,陈伟,黄开华,2016.不同培养基人工培育蝉花中有效成分的比较.上海农业学报,2016(5):101-104