芽孢杆菌aiiA基因抗病研究进展
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摘要
aii A基因编码的Aii A蛋白是一类由芽孢杆菌产生的酰基高丝氨酸内酯酶,能够专一性地水解革兰氏阴性菌分泌的AHLs信号分子,从而破坏依赖群体感应系统的致病机制,减轻病原菌的致病性。综述了aii A基因与Aii A蛋白的研究现状、aii A基因在抗病方面的研究进展及Aii A蛋白在工业发展的应用现状等,以期为aii A基因在更多领域的应用提供理论基础。
Aii A protein encoded by aii A gene is a kind of acyl-homoserine lactonase produced by Bacillus species that can exclusively hydrolyse the AHLs signal molecules secreted by gram negative bacteria that destroy pathogenic mechanism which depend on the quorum sensing system and reduce the pathogenicity of the pathogen. The essay comprehensively analyzed the research situations of aii A and Aii A, the current research progress of aii A in disease-resistant and the application status of Aii A protein in industrial development, which can provide aii A gene with theoretical basis on more fields of application.
Aii A protein encoded by aii A gene is a kind of acyl-homoserine lactonase produced by Bacillus species that can exclusively hydrolyse the AHLs signal molecules secreted by gram negative bacteria that destroy pathogenic mechanism which depend on the quorum sensing system and reduce the pathogenicity of the pathogen. The essay comprehensively analyzed the research situations of aii A and Aii A, the current research progress of aii A in disease-resistant and the application status of Aii A protein in industrial development, which can provide aii A gene with theoretical basis on more fields of application.
引文
[1]Fuqua W C,Winans S C,Greenberg E P.Quorum sensing in bacteria:the Lux R-Lux family of cell density-responsive transcriptional regulators[J].Journal of Bacteriology,1994,176(2):269.
[2]Schauder S,Bassler B L.The languages of bacteria[J].Genes&Development,2001,15(12):1 468-1 480.
[3]Lin Y H,Xu J L,Hu J,et al.Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes[J].Molecular Microbiology,2003,47(3):849-860.
[4]Taga M E,Bassler B L.Chemical communication among bacteria[J].Proceedings of the National Academy of Sciences,2003,100:14 549-14 554.
[5]Zhang L H,Dong Y H.Quorum sensing and signal interference:diverse implications[J].Molecular Microbiology,2004,53(6):1 563-1 571.
[6]吴红,宋志军.细菌与细菌之间的信息交流——革兰氏阴性细菌的Quorum-Sensing系统[J].自然科学进展,2003,13(7):679-687.
[7]Ulrich R L.Quorum quenching:enzymatic disruption of Nacylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis[J].Applied and Environmental Microbiology,2004,70(10):6 173-6 180.
[8]Kjelleberg S,Steinberg P,Givskov M C,et al.Do marine natural products interfere with prokaryotic AHL regulatory systems?[J].Aquatic Microbial Ecology,1997,13(1):85-93.
[9]Dong Y H,Xu J L,Li X Z,et al.Aii A,an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora[J].Proceedings of the National Academy of Sciences,2000,97(7):3 526-3 531.
[10]Dong Y H,Gusti A R,Zhang Q,et al.Identification of quorum-quenching N-acylhomoserine lactonases from Bacillus species[J].Applied and Environmental Microbiology,2002,68(4):1 754-1 759.
[11]周燚,孙明,喻子牛.苏云金芽孢杆菌Aii A蛋白对魔芋软腐病菌的抗病活性[J].武汉大学学报:理学版,2004,50(6):761-764.
[12]王艳敏.AHL酶基因的克隆及序列分析[D].天津:河北工业大学,2005.
[13]黄天培,杨梅,姚帆,等.蜡质芽孢杆菌aii A基因的克隆及融合表达[J].福建农林大学学报:自然科学版,2006,35(3):292-297.
[14]黄天培,姚帆,潘洁茹,等.枯草芽孢杆菌新抗病基因aii A的克隆[J].福建农林大学学报:自然科学版,2007,36(3):269-273.
[15]黄天培.苏云金芽孢杆菌的重要功能基因[D].福州:福建农林大学,2006.
[16]丁贤,孙威文,张殿昌,等.海洋微生物ZD02信号降解酶基因aii A克隆及其生物信息学分析[J].生态学杂志,2013(8):2 056-2 061.
[17]Khoiri S,Tri A D,Giyanto G.Identification of quorum quenching bacteria and its biocontrol potential against soft rot disease bacteria,Dickeya dadantii[J].Agrivita,2017,39(1):45-55.
[18]黄天培,潘洁茹,姚帆,等.蜡质芽孢杆菌酰基高丝氨酸内酯酶基因的克隆及序列分析[J].武夷科学,2008(4):374-378.
[19]韩丽伟,屈淑平,崔崇士.aii A基因在植物软腐病研究中的应用进展[J].中国蔬菜,2011(8):1-6.
[20]Liu D,Lepore B W,Petsko G A,et al.Three-dimensional structure of the quorum-quenching N-acyl homoserine lactone hydrolase from Bacillus thuringiensis[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(33):11 882-11 887.
[21]温晓芳.aii A基因在大肠杆菌中的表达及其活性检测[D].广州:华南热带农业大学,2005.
[22]Perombelon M C M,Kelman A.Ecology of the soft rot Erwinias[J].Annual Review of Phytopathology,1980,18(1):361-387.
[23]Austin B,Austin D A.Bacterial fish pathogens:diseases of farmed and wild fish.4th edition[J].UK:Praxis Publishing Ltd,2007.
[24]王瑶,戴岳,张勇,等.群体感应信号分子降解基因对铜绿假单胞菌毒力和生物膜形成的影响[J].中国科学:C辑,2007,37(2):234-240.
[25]杨梅,姚帆,黄勤清,等.不同来源的苏云金芽孢杆菌aii A基因表达及其抗病性分析[J].应用与环境生物学报,2007,13(3):373-381.
[26]杨梅,郭丽清,关夏玉,等.Aii A融合蛋白包涵体变性和复性的研究[J].福建师范大学学报:自然科学版,2008,24(4):76-79.
[27]杨梅,张锋,林彬辉,等.Aii A蛋白的可溶性表达及其抗菌活性研究[J].分子细胞生物学报,2008,41(6):465-472.
[28]朱晨光,孙明,喻子牛.带cry3Aa启动子的aii A基因在苏云金芽孢杆菌中的表达[J].生物工程学报,2003,19(4):397-401.
[29]吴怀光,叶伟星,喻子牛,等.在苏云金芽孢杆菌中高效和稳定表达Aii A蛋白[J].中国农业科学,2007,40(10):2 221-2 226.
[30]Molina L,Constantinescu F,Michel L,et al.Degradation of pathogen quorum sensing molecules by soil bacteria:a preventive and curative biological control mechanism[J].FEMS Microbiology Ecology,2003,45(1):71-81.
[31]张霞,胡玉琴,贾振华,等.含苏云金芽孢杆菌aii A基因的重组荧光假单胞菌的构建及对软腐病的抗病活性[J].华北农学报,2006,21(4):13-16.
[32]娄瑞娟,张霞,罗利龙,等.aii A基因表达载体的构建及其在毕赤酵母中的表达[J].微生物学通报,2010,37(5):658-663.
[33]曾世涌,苏小惠,谢盼盼,等.苏云金芽孢杆菌中aii A基因密码子偏好性分析[J].生物技术进展,2013,3(4):257-263.
[34]简思美,何晶晶,毛惠民,等.苏云金芽孢杆菌N-酰基高丝氨酸内酯酶基因(aii A)在毕赤酵母中的优化表达[J].农业生物技术学报,2014,22(11):1 347-1 356.
[35]简思美,蔡斌斌,吴程,等.基因改造Aii A蛋白在毕赤酵母中的表达[J].福建师范大学学报:自然科学版,2015,31(6):55-63.
[36]Dong Y H,Wang L H,Xu J L,et al.Quenching quorumsensing-dependent bacterial infection by an N-acyl homoserine lactonase[J].Nature,2001,411(6 839):813-817.
[37]柴鑫莉,周盈,林拥军,等.苏云金芽孢杆菌抗软腐病aii A基因转花魔芋研究[J].分子植物育种,2007,5(5):613-618.
[38]Ban H,Chai X,Lin Y,et al.Transgenic Amorphophallus konjac expressing synthesized acyl-homoserine lactonase(aii A)gene exhibit enhanced resistance to soft rot disease[J].Plant Cell Reports,2009,28(12):1 847-1 855.
[39]班慧芳.转aii A基因魔芋的软腐病抗性及魔芋内生菌产生抑菌蛋白的组成分析[D].武汉:华中农业大学,2009.
[40]陈磊,廖甜甜,郭政宏,等.抗软腐病基因在花魔芋块茎组织中特异表达的研究[J].分子植物育种,2014,12(6):1 230-1 234.
[41]潘丽晶,张妙彬,梁擎中,等.苏云金芽孢杆菌aii A基因载体构建及石斛兰转化[J].中山大学学报:自然科学版,2009,48(1):67-71.
[42]肖杨.双价基因转化石斛兰的研究[D].广州:中山大学,2010.
[43]Ouyang L J,Li L M.Effects of an inducible aii A[J].Transgenic Research,2016,25(4):441-452.
[44]韩丽伟.花粉管通道法介导aii A和Tu MV-CP基因转化大白菜的研究[D].哈尔滨:东北农业大学,2011.
[45]张言朝,葛宇,于杨,等.南瓜子房注射法遗传转化的研究[J].中国蔬菜,2012(16):27-31.
[46]Rajesh P S,Rai V R.Use of aii A gene amplification for AHL-lactonase production from endophytic bacterium Enterobacter species[J].International Journal of Biological Macromolecules,2015,72:1 013-1 019.
[47]Beladiya C,Tripathy R K,Bajaj P,et al.Expression,purification and immobilization of recombinant Aii A enzyme onto magnetic nanoparticles[J].Protein Expression and Purification,2015,113:56-62.
[2]Schauder S,Bassler B L.The languages of bacteria[J].Genes&Development,2001,15(12):1 468-1 480.
[3]Lin Y H,Xu J L,Hu J,et al.Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes[J].Molecular Microbiology,2003,47(3):849-860.
[4]Taga M E,Bassler B L.Chemical communication among bacteria[J].Proceedings of the National Academy of Sciences,2003,100:14 549-14 554.
[5]Zhang L H,Dong Y H.Quorum sensing and signal interference:diverse implications[J].Molecular Microbiology,2004,53(6):1 563-1 571.
[6]吴红,宋志军.细菌与细菌之间的信息交流——革兰氏阴性细菌的Quorum-Sensing系统[J].自然科学进展,2003,13(7):679-687.
[7]Ulrich R L.Quorum quenching:enzymatic disruption of Nacylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis[J].Applied and Environmental Microbiology,2004,70(10):6 173-6 180.
[8]Kjelleberg S,Steinberg P,Givskov M C,et al.Do marine natural products interfere with prokaryotic AHL regulatory systems?[J].Aquatic Microbial Ecology,1997,13(1):85-93.
[9]Dong Y H,Xu J L,Li X Z,et al.Aii A,an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora[J].Proceedings of the National Academy of Sciences,2000,97(7):3 526-3 531.
[10]Dong Y H,Gusti A R,Zhang Q,et al.Identification of quorum-quenching N-acylhomoserine lactonases from Bacillus species[J].Applied and Environmental Microbiology,2002,68(4):1 754-1 759.
[11]周燚,孙明,喻子牛.苏云金芽孢杆菌Aii A蛋白对魔芋软腐病菌的抗病活性[J].武汉大学学报:理学版,2004,50(6):761-764.
[12]王艳敏.AHL酶基因的克隆及序列分析[D].天津:河北工业大学,2005.
[13]黄天培,杨梅,姚帆,等.蜡质芽孢杆菌aii A基因的克隆及融合表达[J].福建农林大学学报:自然科学版,2006,35(3):292-297.
[14]黄天培,姚帆,潘洁茹,等.枯草芽孢杆菌新抗病基因aii A的克隆[J].福建农林大学学报:自然科学版,2007,36(3):269-273.
[15]黄天培.苏云金芽孢杆菌的重要功能基因[D].福州:福建农林大学,2006.
[16]丁贤,孙威文,张殿昌,等.海洋微生物ZD02信号降解酶基因aii A克隆及其生物信息学分析[J].生态学杂志,2013(8):2 056-2 061.
[17]Khoiri S,Tri A D,Giyanto G.Identification of quorum quenching bacteria and its biocontrol potential against soft rot disease bacteria,Dickeya dadantii[J].Agrivita,2017,39(1):45-55.
[18]黄天培,潘洁茹,姚帆,等.蜡质芽孢杆菌酰基高丝氨酸内酯酶基因的克隆及序列分析[J].武夷科学,2008(4):374-378.
[19]韩丽伟,屈淑平,崔崇士.aii A基因在植物软腐病研究中的应用进展[J].中国蔬菜,2011(8):1-6.
[20]Liu D,Lepore B W,Petsko G A,et al.Three-dimensional structure of the quorum-quenching N-acyl homoserine lactone hydrolase from Bacillus thuringiensis[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(33):11 882-11 887.
[21]温晓芳.aii A基因在大肠杆菌中的表达及其活性检测[D].广州:华南热带农业大学,2005.
[22]Perombelon M C M,Kelman A.Ecology of the soft rot Erwinias[J].Annual Review of Phytopathology,1980,18(1):361-387.
[23]Austin B,Austin D A.Bacterial fish pathogens:diseases of farmed and wild fish.4th edition[J].UK:Praxis Publishing Ltd,2007.
[24]王瑶,戴岳,张勇,等.群体感应信号分子降解基因对铜绿假单胞菌毒力和生物膜形成的影响[J].中国科学:C辑,2007,37(2):234-240.
[25]杨梅,姚帆,黄勤清,等.不同来源的苏云金芽孢杆菌aii A基因表达及其抗病性分析[J].应用与环境生物学报,2007,13(3):373-381.
[26]杨梅,郭丽清,关夏玉,等.Aii A融合蛋白包涵体变性和复性的研究[J].福建师范大学学报:自然科学版,2008,24(4):76-79.
[27]杨梅,张锋,林彬辉,等.Aii A蛋白的可溶性表达及其抗菌活性研究[J].分子细胞生物学报,2008,41(6):465-472.
[28]朱晨光,孙明,喻子牛.带cry3Aa启动子的aii A基因在苏云金芽孢杆菌中的表达[J].生物工程学报,2003,19(4):397-401.
[29]吴怀光,叶伟星,喻子牛,等.在苏云金芽孢杆菌中高效和稳定表达Aii A蛋白[J].中国农业科学,2007,40(10):2 221-2 226.
[30]Molina L,Constantinescu F,Michel L,et al.Degradation of pathogen quorum sensing molecules by soil bacteria:a preventive and curative biological control mechanism[J].FEMS Microbiology Ecology,2003,45(1):71-81.
[31]张霞,胡玉琴,贾振华,等.含苏云金芽孢杆菌aii A基因的重组荧光假单胞菌的构建及对软腐病的抗病活性[J].华北农学报,2006,21(4):13-16.
[32]娄瑞娟,张霞,罗利龙,等.aii A基因表达载体的构建及其在毕赤酵母中的表达[J].微生物学通报,2010,37(5):658-663.
[33]曾世涌,苏小惠,谢盼盼,等.苏云金芽孢杆菌中aii A基因密码子偏好性分析[J].生物技术进展,2013,3(4):257-263.
[34]简思美,何晶晶,毛惠民,等.苏云金芽孢杆菌N-酰基高丝氨酸内酯酶基因(aii A)在毕赤酵母中的优化表达[J].农业生物技术学报,2014,22(11):1 347-1 356.
[35]简思美,蔡斌斌,吴程,等.基因改造Aii A蛋白在毕赤酵母中的表达[J].福建师范大学学报:自然科学版,2015,31(6):55-63.
[36]Dong Y H,Wang L H,Xu J L,et al.Quenching quorumsensing-dependent bacterial infection by an N-acyl homoserine lactonase[J].Nature,2001,411(6 839):813-817.
[37]柴鑫莉,周盈,林拥军,等.苏云金芽孢杆菌抗软腐病aii A基因转花魔芋研究[J].分子植物育种,2007,5(5):613-618.
[38]Ban H,Chai X,Lin Y,et al.Transgenic Amorphophallus konjac expressing synthesized acyl-homoserine lactonase(aii A)gene exhibit enhanced resistance to soft rot disease[J].Plant Cell Reports,2009,28(12):1 847-1 855.
[39]班慧芳.转aii A基因魔芋的软腐病抗性及魔芋内生菌产生抑菌蛋白的组成分析[D].武汉:华中农业大学,2009.
[40]陈磊,廖甜甜,郭政宏,等.抗软腐病基因在花魔芋块茎组织中特异表达的研究[J].分子植物育种,2014,12(6):1 230-1 234.
[41]潘丽晶,张妙彬,梁擎中,等.苏云金芽孢杆菌aii A基因载体构建及石斛兰转化[J].中山大学学报:自然科学版,2009,48(1):67-71.
[42]肖杨.双价基因转化石斛兰的研究[D].广州:中山大学,2010.
[43]Ouyang L J,Li L M.Effects of an inducible aii A[J].Transgenic Research,2016,25(4):441-452.
[44]韩丽伟.花粉管通道法介导aii A和Tu MV-CP基因转化大白菜的研究[D].哈尔滨:东北农业大学,2011.
[45]张言朝,葛宇,于杨,等.南瓜子房注射法遗传转化的研究[J].中国蔬菜,2012(16):27-31.
[46]Rajesh P S,Rai V R.Use of aii A gene amplification for AHL-lactonase production from endophytic bacterium Enterobacter species[J].International Journal of Biological Macromolecules,2015,72:1 013-1 019.
[47]Beladiya C,Tripathy R K,Bajaj P,et al.Expression,purification and immobilization of recombinant Aii A enzyme onto magnetic nanoparticles[J].Protein Expression and Purification,2015,113:56-62.