摘要
目的探讨microRNA-196a2基因多态性(rs1614913)与中国人群肺癌发病相关性。方法应用高分辨率溶解曲线(high resolution melting,HRM)分析技术对32例肺癌患者、27例肺部良性肿瘤患者及84例健康个体的microRNA-196a2基因多态性进行分析,并采用聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polymorphism,PCRRFLP)技术加以佐证。结果 micro-196a2在各组基因型分布均达到Hardy-Weinberg平衡。肺癌组C等位基因的分布频率高于对照组(P=0.0016),OR=2.592(95%CI1.424~4.716),说明C等位基因增加肺癌患病风险。结论 MicroRNA-196a2T/C(rs1614913)多态性增加肺癌的发病风险。MicroRNA-196a2基因多态性rs1614913可能是肺癌的一种有效的遗传标记物。
Objective To investigate the effect of the microRNA-1962 gene polymorphism on risk of lung cancer.MethodsThirty-two patients with lung cancer,27 patients with benign neoplasm in lung and 84 healthy controls.All subjects were genotyped for the microRNA-196a2 SNP by high resolution melting analysis,and the results were supported by PCR-restriction fragment length polymorphism method.Results The microRNA 1962 agenotype distribution among three group was in Hardyweinberg equilibrium.The frequency of the C allele in group of lung cancer was higher than that of the control group(P=0.0016).The odds ratio 2.592(95% CI 1.424~4.716)suggested an association of the C allele with increased risk of lung cancer.Conclusions The microRNA-196a2T/C polymorphism(rs1614913)is associated with an increased risk of lung cancer.The miRNA-196a2 functional polymorphism rs11614913 might be an effective genetic marker for lung cancer.
引文
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