HPLC法同时测定不同产地金花葵中6个成分的含量
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摘要
目的建立同时测定金花葵中绿原酸、芦丁、金丝桃苷、异槲皮苷、杨梅素和槲皮素6个成分含量的高效液相色谱法。方法采用Venusil XBP C18色谱柱(250 mm×4. 6 mm,5μm),以体积分数0. 1%甲酸水(A)-乙腈(B)为流动相,梯度洗脱,流速为1. 0 mL·min~(-1),检测波长为355 nm,柱温为25℃。结果绿原酸、芦丁、金丝桃苷、异槲皮苷、杨梅素和槲皮素分别在质量浓度0. 19～12. 42 mg·L~(-1)、0. 42～26. 64 mg·L~(-1)、3. 94～252. 0 mg·L~(-1)、2. 32～148. 4 mg·L~(-1)、0. 52～33. 12 mg·L~(-1)、0. 188～12. 06 mg·L~(-1)内呈现良好的线性关系(r≥0. 999 5),平均加样回收率(n=6)分别为100. 5%、99. 72%、99. 77%、99. 10%、99. 97%和98. 89%,RSD分别为2. 86%、1. 65%、1. 96%、1. 70%、2. 74%和1. 51%;不同产地金花葵中上述6个成分含量分别为0. 007 3%～0. 0261%、0. 015 9%～0. 074 8%、0. 648 3%～1. 076 3%、0. 196%～0. 582 0%、0. 038 8%～0. 118 5%和0. 020 2%～0. 052 7%。结论建立的方法能满足金花葵中6个成分含量的准确测定,为金花葵的质量评价以及合理开发提供参考。
Objective To develop an HPLC method for simultaneous determination of chlorogenic acid,rutin,hyperoside,isoquercitrin,myricetin and quercetin in Aurea Helianthus. Methods An HPLC method for quantitative analysis was applied with an Venusil XBP C18(L) column(250 mm × 4. 6 mm,5 μm) using gradient elution with 0. 1% aqueous formic acid( A)-acetonitrile( B) as the mobile phase at the flowrate of1. 0 mL·min~(-1),the determination wavelength was 355 nm,and the column temperature was set at 25 ℃.Results Good calibration linearity was achieved for chlorogenic acid,rutin,hyperoside,isoquercitrin,myricetin and quercetin in the ranges of 0. 19-12. 42 mg ·L~(-1),0. 42-26. 64 mg·L~(-1),3. 94-252. 0 mg·L~(-1),2. 32-148. 4 mg·L~(-1),0. 52-33. 12 mg·L~(-1) and 0. 188-12. 06 mg·L~(-1),respectively(r≥0. 999 5). The average recoveries( n = 6) were 100. 5% with RSD of 2. 86%,99. 72% with RSD of1. 65%,99. 77% with RSD of 1. 96%,99. 10% with RSD of 1. 70%,99. 97% with RSD of 2. 74%,98. 89% with RSD of 1. 51%,respectively. The contents of 6 components in Aurea Helianthus from different habitats were 0. 007 3%-0. 026 1%,0. 015 9%-0. 074 8%,0. 648 3%-1. 076 3%,0. 196%-0. 582 0%,0. 038 8%-0. 118 5%,0. 020 2%-0. 052 7%. Conclusion The developed method is simple,well separated and precise,which can be used to determine the six kinds of content in Aurea Helianthus,and provide reference for quality evaluation and reasonable exploitation of Aurea Helianthus.
Objective To develop an HPLC method for simultaneous determination of chlorogenic acid,rutin,hyperoside,isoquercitrin,myricetin and quercetin in Aurea Helianthus. Methods An HPLC method for quantitative analysis was applied with an Venusil XBP C18(L) column(250 mm × 4. 6 mm,5 μm) using gradient elution with 0. 1% aqueous formic acid( A)-acetonitrile( B) as the mobile phase at the flowrate of1. 0 mL·min~(-1),the determination wavelength was 355 nm,and the column temperature was set at 25 ℃.Results Good calibration linearity was achieved for chlorogenic acid,rutin,hyperoside,isoquercitrin,myricetin and quercetin in the ranges of 0. 19-12. 42 mg ·L~(-1),0. 42-26. 64 mg·L~(-1),3. 94-252. 0 mg·L~(-1),2. 32-148. 4 mg·L~(-1),0. 52-33. 12 mg·L~(-1) and 0. 188-12. 06 mg·L~(-1),respectively(r≥0. 999 5). The average recoveries( n = 6) were 100. 5% with RSD of 2. 86%,99. 72% with RSD of1. 65%,99. 77% with RSD of 1. 96%,99. 10% with RSD of 1. 70%,99. 97% with RSD of 2. 74%,98. 89% with RSD of 1. 51%,respectively. The contents of 6 components in Aurea Helianthus from different habitats were 0. 007 3%-0. 026 1%,0. 015 9%-0. 074 8%,0. 648 3%-1. 076 3%,0. 196%-0. 582 0%,0. 038 8%-0. 118 5%,0. 020 2%-0. 052 7%. Conclusion The developed method is simple,well separated and precise,which can be used to determine the six kinds of content in Aurea Helianthus,and provide reference for quality evaluation and reasonable exploitation of Aurea Helianthus.
引文
[1]卢丹,贾瑞波.中药金花葵的研究进展[J].中国药物评价,2015,32(2):90-92.
[2]孔静,贺晶,谭立,等.基于金花葵核苷类成分的质量表征关联分析研究[J].北京中医药大学学报,2017,40(5):420-427.
[3]张路,于慧敏,杜凤沛,等.金花葵中总黄酮的分离提取及含量测定[J].吉林师范大学学报(自然科学版),2015,26(4):104-108.
[4]张建.金花葵花总黄酮解热抗炎作用的实验研究[J].中国医科大学学报,2011,61(8):763-764.
[5]吴正平.金花葵花总黄酮镇痛作用的实验研究[J].宜春学院学报,2009,31(4):75-76.
[6]吴正平.金花葵花总黄酮对衰老模型小鼠抗氧化和免疫功能的影响[J].中国老年学杂志,2011,31(10):1800-1801.
[7]杨秀松.金花葵粗黄酮提取物的免疫调节作用研究[J].中国药师,2013,16(9):1307-1310.
[8]林萍,易宏伟,张斐.金丝桃苷药理作用研究进展[J].中国现代中药,2012,14(10):23-26.
[9]唐敏,刘耀,夏培元.金丝桃苷对CCl4诱导的大鼠肝损伤的保护作用研究[J].中国药房,2011,22(7):582-583.
[10]白云,朱瑞超,李晋,等.超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量[J].天津中医药,2018,35(2):147-150.
[11]冯朵,谭立,彭平,等.基于药物体系的金花葵特征图谱质量表征关联分析研究[J].北京中医药大学学报,2016,39(4):308-315.
[12]易剑峰. HPLC法测定金花葵花中金丝桃苷的含量[J].宜春学院学报,2007,25(4):72,97.
[13]曹东怡,虞泓,曾文波,等.金花葵不同部位化学成分分析[J].安徽农业科学,2017,45(22):90-92,109.
[14]邱金玲,刘朋,熊维政,等.金花葵中金丝桃苷的含量测定[J].中医学报,2015,30(12):1793-1795.
[2]孔静,贺晶,谭立,等.基于金花葵核苷类成分的质量表征关联分析研究[J].北京中医药大学学报,2017,40(5):420-427.
[3]张路,于慧敏,杜凤沛,等.金花葵中总黄酮的分离提取及含量测定[J].吉林师范大学学报(自然科学版),2015,26(4):104-108.
[4]张建.金花葵花总黄酮解热抗炎作用的实验研究[J].中国医科大学学报,2011,61(8):763-764.
[5]吴正平.金花葵花总黄酮镇痛作用的实验研究[J].宜春学院学报,2009,31(4):75-76.
[6]吴正平.金花葵花总黄酮对衰老模型小鼠抗氧化和免疫功能的影响[J].中国老年学杂志,2011,31(10):1800-1801.
[7]杨秀松.金花葵粗黄酮提取物的免疫调节作用研究[J].中国药师,2013,16(9):1307-1310.
[8]林萍,易宏伟,张斐.金丝桃苷药理作用研究进展[J].中国现代中药,2012,14(10):23-26.
[9]唐敏,刘耀,夏培元.金丝桃苷对CCl4诱导的大鼠肝损伤的保护作用研究[J].中国药房,2011,22(7):582-583.
[10]白云,朱瑞超,李晋,等.超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量[J].天津中医药,2018,35(2):147-150.
[11]冯朵,谭立,彭平,等.基于药物体系的金花葵特征图谱质量表征关联分析研究[J].北京中医药大学学报,2016,39(4):308-315.
[12]易剑峰. HPLC法测定金花葵花中金丝桃苷的含量[J].宜春学院学报,2007,25(4):72,97.
[13]曹东怡,虞泓,曾文波,等.金花葵不同部位化学成分分析[J].安徽农业科学,2017,45(22):90-92,109.
[14]邱金玲,刘朋,熊维政,等.金花葵中金丝桃苷的含量测定[J].中医学报,2015,30(12):1793-1795.