叶面喷施水杨酸对当归幼苗生理特性和保护酶活性的影响
|
摘要
目的:研究叶面喷施水杨酸(SA)对当归幼苗生长和保护酶活性的影响,为当归种子种苗的生产提供科学依据。方法:采用叶面喷施的方法,设置不同处理浓度,考察水杨酸对当归幼苗生物量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。结果:叶面喷施适宜浓度的水杨酸能促进当归幼苗的生长并提高保护酶活性。其中以0.5 mmol·L~(-1)水杨酸处理的当归幼苗株高和鲜质量为最高,分别为对照组的135.2%和224.6%,超过此浓度,植株生长受到抑制。1.0 mmol·L~(-1)水杨酸处理的当归幼苗,SOD、POD、CAT酶活性分别为对照组的149.1%(7 d)、138.2%(7 d)、194.7%(5 d)。结论:叶面喷施0.5 mmol·L~(-1)的水杨酸能够促进当归幼苗生长,较高浓度的水杨酸对提高当归幼苗SOD、POD、CAT酶活性的效果较好,但会加剧对植株生长的抑制作用。
Objective:The study aims at investigating the influences of exogenous salicylic acid on the growth and antioxidase activity of Angelica sinensis seedlings,and providing a theoretical basis for breeding.Methods:Foliar spraying with a series concentration of salicylic acid were applied.The length and fresh weight,superoxide dismutase,peroxidase and catalase activities of the seedlings were measured after salicylic acid treatments.Results:The growth and antioxidase activity of A.sinensis seedlings could be promoted with appropriate concentration of salicylic acid treatments.The length and fresh weight of the seedlings was increased to 135.2% and 224.6% compared with the control after 0.5 mmol·L~(-1) salicylic acid spraying respectively.However,the growth conditions and weights of seedlings were inhibited by increasing concentration of salicylic acid.SOD,POD and CAT activities were promoted to 149.1%(7 d),138.2%(7 d),and 194.7%(5 d)compared with controls respectively under the treatment of 1.0 mmol·L~(-1) salicylic acid.Conclusion:0.5 mmol·L~(-1) of salicylic acid foliar spraying is benefit for growth condition of seedlings,and relatively higher salicylic acid concentration will be benefit to antioxidase activities promotion,but inhibits the growth condition of A.sinensis seedlings apparently.
Objective:The study aims at investigating the influences of exogenous salicylic acid on the growth and antioxidase activity of Angelica sinensis seedlings,and providing a theoretical basis for breeding.Methods:Foliar spraying with a series concentration of salicylic acid were applied.The length and fresh weight,superoxide dismutase,peroxidase and catalase activities of the seedlings were measured after salicylic acid treatments.Results:The growth and antioxidase activity of A.sinensis seedlings could be promoted with appropriate concentration of salicylic acid treatments.The length and fresh weight of the seedlings was increased to 135.2% and 224.6% compared with the control after 0.5 mmol·L~(-1) salicylic acid spraying respectively.However,the growth conditions and weights of seedlings were inhibited by increasing concentration of salicylic acid.SOD,POD and CAT activities were promoted to 149.1%(7 d),138.2%(7 d),and 194.7%(5 d)compared with controls respectively under the treatment of 1.0 mmol·L~(-1) salicylic acid.Conclusion:0.5 mmol·L~(-1) of salicylic acid foliar spraying is benefit for growth condition of seedlings,and relatively higher salicylic acid concentration will be benefit to antioxidase activities promotion,but inhibits the growth condition of A.sinensis seedlings apparently.
引文
[1] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:133-134.
[2] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1992,55(3):41-43.
[3] 武延安,陈秀实.古本草中当归产地的商榷与新考[J].中国现代中药,2016,18(10):1354-1358.
[4] 尹玲莉,侯晓杰.植物抗性信号分子—水杨酸研究进展[J].中国农学通报,2007,23(1):338-342.
[5] CHEN Y E,CUI J M,LI G X,et al.Effect of salicylic acid on the antioxidant system and photosystem II in wheat seedlings[J].Biol Plant,2016,60(1):139-147.
[6] 李天来,李淼,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3):586-590.
[7] 宋士清,郭世荣,尚庆茂,等.外源SA对盐胁迫下黄瓜幼苗的生理效应[J].园艺学报,2006,33(1):68-72.
[8] 王楠,高静,黄文静,等.旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应[J].草业科学,2018,35(1):106-114.
[9] 王春丽,梁宗锁,李殿荣,等.茉莉酸甲酯和水杨酸对丹参幼苗中蔗糖代谢和酚酸类物质积累的影响[J].西北植物学报,2011,31(7):1405-1410.
[10] WANG X M,YANG B,REN C G,et al.Involvement of abscisic acid and salicylic acid in signal cascade regulating bacterial endophyt-induced volatile oil biosynthesis in plantlets of Atractylodes lancea[J].Physiol Plant,2015,153(1):30-42.
[11] GIMéNEZ M J,SERRANO M,VALVERDE J M,et al.Preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars[J].J Sci Food Agric,2017,97(4):1220-1228.
[12] YIN Y Q,QI F,GAO L,et al.iTRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress[J].RSC Adv,2018,8(32):17905-17913.
[2] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1992,55(3):41-43.
[3] 武延安,陈秀实.古本草中当归产地的商榷与新考[J].中国现代中药,2016,18(10):1354-1358.
[4] 尹玲莉,侯晓杰.植物抗性信号分子—水杨酸研究进展[J].中国农学通报,2007,23(1):338-342.
[5] CHEN Y E,CUI J M,LI G X,et al.Effect of salicylic acid on the antioxidant system and photosystem II in wheat seedlings[J].Biol Plant,2016,60(1):139-147.
[6] 李天来,李淼,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3):586-590.
[7] 宋士清,郭世荣,尚庆茂,等.外源SA对盐胁迫下黄瓜幼苗的生理效应[J].园艺学报,2006,33(1):68-72.
[8] 王楠,高静,黄文静,等.旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应[J].草业科学,2018,35(1):106-114.
[9] 王春丽,梁宗锁,李殿荣,等.茉莉酸甲酯和水杨酸对丹参幼苗中蔗糖代谢和酚酸类物质积累的影响[J].西北植物学报,2011,31(7):1405-1410.
[10] WANG X M,YANG B,REN C G,et al.Involvement of abscisic acid and salicylic acid in signal cascade regulating bacterial endophyt-induced volatile oil biosynthesis in plantlets of Atractylodes lancea[J].Physiol Plant,2015,153(1):30-42.
[11] GIMéNEZ M J,SERRANO M,VALVERDE J M,et al.Preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars[J].J Sci Food Agric,2017,97(4):1220-1228.
[12] YIN Y Q,QI F,GAO L,et al.iTRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress[J].RSC Adv,2018,8(32):17905-17913.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |