CTCL患者外周血淋巴细胞亚群、血清sIL-2R及炎性因子的水平变化特点及意义
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摘要
目的:探讨皮肤T细胞淋巴瘤(CTCL)患者外周血淋巴细胞亚群及血清可溶性白细胞介素2受体(sIL-2R)、炎性因子的水平变化特点及意义。方法:选取2013年1月至2018年1月在我院治疗的CTCL患者35例(CTCL组),同时选取炎症性皮肤病患者35例作为对照组,检测两组血清sIL-2R、B淋巴细胞、T淋巴细胞和NK细胞水平以及白细胞介素-6(IL-6)、肿瘤坏死因子(TNF-α)和C反应蛋白(CRP)。结果:CTCL组血清sIL-2R为(770.15±87.44)U/ml,明显高于对照组(P<0.05),而B淋巴细胞为6.10(0.15～10.57)%,明显低于对照组(P<0.05);CTCL组和对照组T淋巴细胞和NK细胞比较差异无统计学意义(P>0.05);CTCL组血清IL-6、TNF-α和CRP分别为110.02(67.39,230.03)pg/ml、(31.29±7.28)pg/ml和(36.60±6.10)mg/L,明显高于对照组(P<0.05);皮损改善患者治疗后sIL-2R为(230.04±78.11)U/ml,明显低于皮损未改善患者(P<0.05)。结论:CTCL患者血清sIL-2R、IL-6、TNF-α和CRP升高,而B淋巴细胞水平降低,其中sIL-2R与治疗效果有一定关系,值得进一步研究。
Objective:To investigate the level and significance of peripheral blood lymphocyte subsets and serum soluble interleukin 2 receptor(sIL-2 R) and inflammatory factors in patients with cutaneous T cell lymphoma(CTCL).Methods:From January 2013 to January 2018,35 CTCL patients(group CTCL) were treated in our hospital,and 35 patients with inflammatory dermatosis were selected as control group.The levels of serum sIL-2 R,B lymphocyte,T lymphocyte and NK cells and interleukin-6(IL-6),tumor necrosis factor(TNF-α) and C reactive protein(CRP) were detected in two groups.Results:The serum sIL-2 R in CTCL group was(770.15±87.44)U/ml,which was significantly higher than that in the control group(P<0.05),while the B lymphocyte was 6.10(0.15 ～10.57)%,which was significantly lower than that of the control group(P<0.05).There were no significant difference in T lymphocyte and NK cell between CTCL group and control group(P>0.05).The serum IL-6,TNF-α and CRP in the CTCL group were 110.02(67.39,230.03)pg/ml,(31.29±7.28)pg/ml and(36.60±6.10)mg/L,which were significantly higher than those in the control group(P<0.05).The sIL-2 R of patients with skin lesions improved after treatment was(230.04±78.11)U/ml,which was significantly lower than that of patients whose skin lesions were not improved(P<0.05).Conclusion:The serum levels of sIL-2 R,IL-6,TNF-α and CRP in patients with CTCL are increased,and the level of B lymphocytes are decreased,and sIL-2 R is related to the effect of treatment,which is worth further study.
Objective:To investigate the level and significance of peripheral blood lymphocyte subsets and serum soluble interleukin 2 receptor(sIL-2 R) and inflammatory factors in patients with cutaneous T cell lymphoma(CTCL).Methods:From January 2013 to January 2018,35 CTCL patients(group CTCL) were treated in our hospital,and 35 patients with inflammatory dermatosis were selected as control group.The levels of serum sIL-2 R,B lymphocyte,T lymphocyte and NK cells and interleukin-6(IL-6),tumor necrosis factor(TNF-α) and C reactive protein(CRP) were detected in two groups.Results:The serum sIL-2 R in CTCL group was(770.15±87.44)U/ml,which was significantly higher than that in the control group(P<0.05),while the B lymphocyte was 6.10(0.15 ～10.57)%,which was significantly lower than that of the control group(P<0.05).There were no significant difference in T lymphocyte and NK cell between CTCL group and control group(P>0.05).The serum IL-6,TNF-α and CRP in the CTCL group were 110.02(67.39,230.03)pg/ml,(31.29±7.28)pg/ml and(36.60±6.10)mg/L,which were significantly higher than those in the control group(P<0.05).The sIL-2 R of patients with skin lesions improved after treatment was(230.04±78.11)U/ml,which was significantly lower than that of patients whose skin lesions were not improved(P<0.05).Conclusion:The serum levels of sIL-2 R,IL-6,TNF-α and CRP in patients with CTCL are increased,and the level of B lymphocytes are decreased,and sIL-2 R is related to the effect of treatment,which is worth further study.
引文
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[14]Li Gangcan,Song Yanping,Zhang Yunjie,et al.Effects of mesenchymal stem cells on hematopoietic support and T lymphocyte secretion in patients with aplastic anemia[J].Chinese Journal of Tissue Engineering Research,2016,20(1):107-112.[李罡灿,宋艳萍,张韵洁,等.间充质干细胞对再生障碍性贫血患者造血支持及T淋巴细胞分泌功能的影响[J].中国组织工程研究,2016,20(1):107-112.]
[2]Jiang W,Abou YZ,Dabaja B,et al.CD4:CD8 ratio in mycosis fungoides:Prognostic and predictive value of circulating T lymphocyte subsets for outcomes after total skin electron beam radiation[J].International Journal of Radiation Oncology Biology Physics,2016,96(2):S20.
[3]Yu Kai,Wang Yiyu,Jin Xianhua,et al.Effects of acitretin and interferon on proliferation and expression of interleukin-15 in human cutaneous T-cell lymphoma cell line Hut78[J].Chinese Journal of Dermatology,2016,49(11):811-815.[于凯,王一宇,金仙花,等.阿维A与干扰素对人皮肤T细胞淋巴瘤细胞株Hut78细胞增殖及白细胞介素15表达的影响[J].中华皮肤科杂志,2016,49(11):811-815.]
[4]Alcasid M,Ma L,Gotlib JR,et al.The clinicopathologic significance of lymphocyte subsets in acute myeloid leukemia[J].International Journal of Laboratory Hematology,2017,39(2):129-136.
[5]Ding Cheng,Wang Ping.Research progress of interleukin 13 and cutaneous T-cell lymphoma[J].Journal of Anhui Medical University,2016,51(9):1388-1390.[丁承,王平.白介素13与皮肤T细胞淋巴瘤的研究进展[J].安徽医科大学学报,2016,51(9):1388-1390.]
[6]Jin E,Li S,Ren M,et al.Boron affects immune function through modulation of splenic T lymphocyte subsets,cytokine secretion,and lymphocyte proliferation and apoptosis in rats[J].Biological Trace Element Research,2017,178(2):1-15.
[7]Kakugawa K,Kojo S,Tanaka H,et al.Essential roles of SATB1 in specifying T lymphocyte subsets[J].Cell Reports,2017,19(6):1176-1188.
[8]Zhang Yanfang,Wu Yanli,Shi Hongmei,et al.Changes of T lymphocyte subsets and NK cells,serum interleukin and TNF-αin children with hand,foot and mouth disease[J].Chinese Journal of Nosocomiology,2016,26(11):2604-2606.[张艳芳,武艳丽,施红梅,等.手足口病患儿T淋巴细胞亚群与NK细胞和血清白介素、TNF-α变化的研究[J].中华医院感染学杂志,2016,26(11):2604-2606.]
[9]Zhou JF,Chen ZY,Yang SM,et al.Clinical features and peripheral blood T lymphocyte subsets in hand,foot,and mouth disease according to different pathogens[J].Indian Journal of Pediatrics,2017,84(2):124-127.
[10]Zhang Xiaona,Li Jidong,Hu Fangjie,et al.The number of spleen T lymphocytes decreased and the immunological activity decreased in high altitude hypoxic exposed mice[J].Journal of Cellular and Molecular Immunology,2017,33(2):164-167.[张晓娜,李积东,胡方杰,等.高原低氧暴露小鼠脾脏T淋巴细胞数量减少且免疫活性下降[J].细胞与分子免疫学杂志,2017,33(2):164-167.]
[11]Emery P,Mcinnes I,Genovese MC,et al.A7.16 Characterisation of changes in lymphocyte subsets in baricitinib-treated patients with rheumatoid arthritis in two phase 3 studies[J].Annals of the Rheumatic Diseases,2016,75(Suppl 1):A62.1-A62.
[12]Shi Yan,Hui Xiaoxia,Liu Ping,et al.Correlation between serum cytokines and soluble interleukin-2 receptors in children with measles and severity of disease[J].Chinese Journal of Nosocomiology,2016,26(15):3551-3553.[石岩,惠小霞,刘平,等.麻疹患儿血清细胞因子和可溶性白细胞介素2受体与病情严重程度的相关性研究[J].中华医院感染学杂志,2016,26(15):3551-3553.]
[13]Sigrid L,Kristoffersen EK,Dipak S,et al.Serum BAFF and A-PRIL levels,T-lymphocyte subsets,and immunoglobulins after B-cell depletion using the monoclonal anti-CD20 antibody rituximab in myalgic encephalopathy/chronic fatigue syndrome[J].Plos One,2016,11(8):e0161226.
[14]Li Gangcan,Song Yanping,Zhang Yunjie,et al.Effects of mesenchymal stem cells on hematopoietic support and T lymphocyte secretion in patients with aplastic anemia[J].Chinese Journal of Tissue Engineering Research,2016,20(1):107-112.[李罡灿,宋艳萍,张韵洁,等.间充质干细胞对再生障碍性贫血患者造血支持及T淋巴细胞分泌功能的影响[J].中国组织工程研究,2016,20(1):107-112.]