归脾汤对化疗相关性疲劳模型TNF-α分泌的调节效应
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摘要
目的:探讨归脾汤对化疗相关性疲劳模型TNF-α分泌的调节效应。方法:6周龄SPF级C57BL/6小鼠,雌雄各半,共80只,随机分为4组,每组20只,即空白对照组(A组)、化疗疲劳组(B组)、香菇多糖组(C组)和归脾汤组(D组)。实验前正常预养1周。将除A组以外的小鼠腹腔注射5-氟尿嘧啶(5-Fu),剂量为40mg/kg,每天给药1次,连续5天。同时,空白对照组给予生理盐水腹腔注射。造模前两天C组给予香菇多糖、D组给予归脾汤灌胃,A组和B组等量生理盐水灌胃,共14天。期间测量体重和抓力。第15天处死动物取腹腔巨噬细胞、脾和胸腺,测定TNF-α的分泌水平。结果:归脾汤可增加小鼠的体重、抓力以及胸腺指数和脾指数,降低TNF-α的分泌水平。结论:归脾汤可改善化疗引起的相关性疲劳,可能与降低TNF-α的分泌有关。
Objective:To investigate the regulatory effect of Guipi Decoction on TNF-αsecretion in chemotherapy-induced fatigue model.Methods:Eighty six-week-old SPF C57 BL/6 mice were randomly divided into four groups,namely normal,group(group A),CIF group(group B),lentinan group(group C)and Guipi Decoction group(group D).The mice were injected 5-fluorouracil(40 mg/kg)i.p.daily for five days except group A,group A was given intraperitoneal injection of saline.At the same time,group C was given lentinan,group D was given Guipi Decoction,and group A and B were given saline for 14 days.Body weight and grasping power were measured during the experimental period.On the 15 th day,the mice were sacrificed to inspect the TNF-αlevels of peritoneal macrophages as well as spleen and thymus index were measured.Results:Guipi Decoction can increase the body weight of mice,grasping power and thymus index and spleen index,decrease the secretion level of TNF-α.Conclusion:Guipi Decoction can improve CIF caused by 5-Fu,may be associated with reduction of the TNF-α Secretion.
Objective:To investigate the regulatory effect of Guipi Decoction on TNF-αsecretion in chemotherapy-induced fatigue model.Methods:Eighty six-week-old SPF C57 BL/6 mice were randomly divided into four groups,namely normal,group(group A),CIF group(group B),lentinan group(group C)and Guipi Decoction group(group D).The mice were injected 5-fluorouracil(40 mg/kg)i.p.daily for five days except group A,group A was given intraperitoneal injection of saline.At the same time,group C was given lentinan,group D was given Guipi Decoction,and group A and B were given saline for 14 days.Body weight and grasping power were measured during the experimental period.On the 15 th day,the mice were sacrificed to inspect the TNF-αlevels of peritoneal macrophages as well as spleen and thymus index were measured.Results:Guipi Decoction can increase the body weight of mice,grasping power and thymus index and spleen index,decrease the secretion level of TNF-α.Conclusion:Guipi Decoction can improve CIF caused by 5-Fu,may be associated with reduction of the TNF-α Secretion.
引文
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[7]赵江涛,王中霞.通窍鼻炎丸治疗慢性鼻窦炎及其对相关炎性因子的影响[J].山东中医杂志,2017,36(8):663-665.
[8]何晓玲,邹凌云,曹瑶,等.不同强度有氧运动对乳腺癌大鼠癌因性疲乏及血清IL-6、TNF-α、IL-1β水平的影响[J].中国康复医学杂志,2015,30(9):872-876.
[2]钱会南.归脾汤临床应用及实验研究现状[J].中国中医药信息杂志,2001(9):23-25.
[3]陈雯明,王光耀.归脾汤新用[J].中国中医药现代远程教育,2015,13(7):154-155.
[4]新昕,季颖.归脾汤对抑郁模型小鼠血清中IL-4的影响[J].实用中医内科杂志,2011,25(5):41-42.
[5]MAHONEY SE,DAVIS JM,MURPHY EA,et al.Dietary quercetin reduces chemotherapy-induced fatigue in mice[J].Integrative Cancer Therapies,2014,13(5):417-424.
[6]刘立,杨欢,许瑞,等.归脾汤对苯中毒小鼠血清中CD3、CD4和CD8的影响[J].时珍国医国药,2014,25(6):1534-1536.
[7]赵江涛,王中霞.通窍鼻炎丸治疗慢性鼻窦炎及其对相关炎性因子的影响[J].山东中医杂志,2017,36(8):663-665.
[8]何晓玲,邹凌云,曹瑶,等.不同强度有氧运动对乳腺癌大鼠癌因性疲乏及血清IL-6、TNF-α、IL-1β水平的影响[J].中国康复医学杂志,2015,30(9):872-876.