菟丝子提取物通过BMP2/Smad4信号通路对MC3T3-E1细胞增殖的影响
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摘要
目的:研究中药菟丝子对MC3T3-E1细胞促增殖作用及其可能的作用机制。方法:体外培养小鼠成骨细胞MC3T3-E1,通过MTT法检测菟丝子提取物对细胞增殖的影响,通过相关试剂盒检测碱性磷酸酶(ALP)活性和钙盐沉积水平,Western Blot检测Ⅰ型胶原蛋白、BMP2和Smad4蛋白表达。结果:菟丝子提取物能够促进小鼠成骨细胞MC3T3-E1增殖,提高ALP活性和钙盐沉积,并上调Col-Ⅰ、BMP2和Smad4蛋白表达(P<0.05),菟丝子提取物浓度在10μg/mL时促进作用较明显。结论:菟丝子提取物能够促进成骨细胞增殖,其作用机制可能与调节BMP2/Smad4信号通路有关。
Objective: To study the proliferation-promoting effects of the extract of dodder seed on MC3 T3-E1 cells and its possible mechanism. Methods: Mouse osteoblasts MC3 T3-E1 cells were cultured in vitro, and the effects of the extract of dodder seed on MC3 T3-E1 cell proliferation was detected by MTT method. Alkaline phosphatase(ALP) activity and the level of calcium salt deposition were detected by relevant kits. The protein expression of type Ⅰ collagen, BMP2 and Smad4 was analyzed by western blot. Results: The extract of dodder seed could promote the proliferation of MC3 T3-E1 cells, enhanced the activity of ALP and calcium salt deposition, and up-regulated expression of Col-Ⅰ, BMP2 and Smad4 protein, the difference was statistically significant(P<0.05). Conclusion: The extract of dodder seed promotes proliferation of osteoblast cells and its mechanism may be related to the regulating BMP2/Smad4 signal pathway.
Objective: To study the proliferation-promoting effects of the extract of dodder seed on MC3 T3-E1 cells and its possible mechanism. Methods: Mouse osteoblasts MC3 T3-E1 cells were cultured in vitro, and the effects of the extract of dodder seed on MC3 T3-E1 cell proliferation was detected by MTT method. Alkaline phosphatase(ALP) activity and the level of calcium salt deposition were detected by relevant kits. The protein expression of type Ⅰ collagen, BMP2 and Smad4 was analyzed by western blot. Results: The extract of dodder seed could promote the proliferation of MC3 T3-E1 cells, enhanced the activity of ALP and calcium salt deposition, and up-regulated expression of Col-Ⅰ, BMP2 and Smad4 protein, the difference was statistically significant(P<0.05). Conclusion: The extract of dodder seed promotes proliferation of osteoblast cells and its mechanism may be related to the regulating BMP2/Smad4 signal pathway.
引文
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[2]郭军,白书阁,王玉民,等.菟丝子抗衰老作用的实验研究.中国老年学杂志,1996,1(1):37-38
[3]郭军,马宏岩,王建军.菟丝子对糖尿病患者抗氧化能力的影响.佳木斯医学院学报,1997,20(1):39-41
[4]Lin M K,Yu Y L,Chen K C,et al.Kaempferol from Semen cuscutae attenuates the immune function of dendritic cells.Immunobiology,2011,216(10):1103-1109
[5]林慧彬,林建强,林建群,等.山东产四种菟丝子免疫增强作用的比较研究.中西医结合学报,2003,1(1):51-53
[6]蔡西国,赵素霞.菟丝子黄酮干预去卵巢大鼠骨代谢研究.中药药理与临床,2007,23(6):27-29
[7]李春霖.老年骨质疏松症的诊断与治疗.西安:中华医学会第十二次全国内分泌学学术会议,2013:112-113
[8]招文华,任辉,沈耿杨,等.骨质疏松症肾阳虚病证结合模型的研究进展.中华中医药杂志,2018,33(1):219-223
[9]李安娜.BMP-Smad信号通路在成骨与破骨细胞生成中的作用.济南:山东大学,2014
[10]李丽莉.BMP-Smad信号通路的调节及其在成骨分化中的作用.济南:山东大学,2013