‘鸭梨’及其自交亲和性芽变‘金坠梨’花粉ABC转运蛋白家族分析
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摘要
自交亲和性的‘金坠梨’是由自交不亲和性的‘鸭梨’芽变而来,为探究‘金坠梨’突变机理,综合分析了二者花粉蛋白质组差异表达蛋白质。在489个差异表达蛋白质中,发现了参与自交不亲和识别机制的ABC转运家族蛋白;KEGG富集分析发现,ABC转运蛋白被显著富集,其中PyABCB9-1,PyABCB9-2,PyABCC2,PyABCC3,PyABCD1,PyABCG31等6个蛋白显著下调;生物信息学分析表明,6个ABC转运蛋白不同家族在物种间具有很高的保守性;这6个显著下调蛋白可能影响了‘金坠梨’花柱S-RNase进入‘自己’花粉管的运输过程而导致其亲和性突变。
‘Jinzhuili’ is a spontaneous self-compatible mutant of ‘Yali’. The mechanism of self-incompatibility in pear was studied at proteome level by using Isbaric Tags for Relative and Absolute Quantitation(iTRAQ). ABC transporters implicated in self-incompatibility recognition mechanism were found among 489 differentially expressed proteins. As a result, ABC transporters were significantly enriched by KEGG. And six down-regulated ABC transporters were obtained, including PyABCB9-1, PyABCB9-2, PyABCC2, PyABCC3, PyABCD1 and PyABCG31. Bioinformatics analysis showed that they had good conservativeness among species. These six ABC transporters might affect the transportation of S-RNase into the pollen tube of ‘Jinzhuili’, and then lead to self-compatible mutation in ‘Jinzhuili’.
‘Jinzhuili’ is a spontaneous self-compatible mutant of ‘Yali’. The mechanism of self-incompatibility in pear was studied at proteome level by using Isbaric Tags for Relative and Absolute Quantitation(iTRAQ). ABC transporters implicated in self-incompatibility recognition mechanism were found among 489 differentially expressed proteins. As a result, ABC transporters were significantly enriched by KEGG. And six down-regulated ABC transporters were obtained, including PyABCB9-1, PyABCB9-2, PyABCC2, PyABCC3, PyABCD1 and PyABCG31. Bioinformatics analysis showed that they had good conservativeness among species. These six ABC transporters might affect the transportation of S-RNase into the pollen tube of ‘Jinzhuili’, and then lead to self-compatible mutation in ‘Jinzhuili’.
引文
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[24] Meng Dong,Gu Zhaoyu,Li Wei,et al.Apple MdABCF assists the transportation of S-RNase into pollen tubes[J].The Plant Journal,2014,78(6):990-1 002.
[25] Choi Hyunju,Jin Junyoung,Choi Setbyoul,et al.An ABCG/WBC-type ABC transporter is essential for transport of sporopollenin precursors for exine formation in developing pollen[J].Plant Journal,2011,65(2):181-193.
[26] Cecchetti V,Brunetti P,Napoli N,et al.ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development[J].Journal of Integrative Plant Biology,2015,57(12):1 089-1 098.
[2] 金炳奎,宗成文,曹后男,等.延边地区梨品种授粉亲和性研究[J].延边大学农学学报,2012,34(3):207-210.
[3] 周俊国,扈惠灵,张慧蓉,等.苹果和梨自交不亲和程度的荧光显微观察[J].河南科技学院学报(自然科学版),2010,38(2):27-31.
[4] Li W,Zhao F,Fang W,et al.Identification of early salt stress responsive proteins in seedling roots of upland cotton(Gossypium hirsutum L.) employing iTRAQ-based proteomic technique[J].Frontiers in Plant Science,2015,6:732.
[5] Eva María Gómez,Federico Dicenta,Pedro José Martínez-García,et al.iTRAQ-based quantitative proteomic analysis of pistils and anthers from self-incompatible and self-compatible almonds with the Sf haplotype[J].Molecular Breeding,2015,35(5):120.
[6] Rudd J J,Franklin F C H,Franklin-Tong V E.Ca2+-independent phosphorylation of a 68 kDa pollen protein is stimulated by the self-incompatibility response in Papaver rhoeas[J].Plant Journal,1997,12(3):507-514.
[7] Qu H,Guan Y,Wang Y,et al.PLC-Mediated Signaling Pathway in Pollen Tubes Regulates the Gametophytic Self-incompatibility of Pyrus Species[J].Frontiers in Plant Science,2017,8:1 164.
[8] 王海静,李明媛,武军凯,等.‘鸭梨’及其自交亲和性芽变‘金坠梨’花粉蛋白质组病原防御相关蛋白表达分析[J].河北科技师范学院学报,2018,32(1):1-8.
[9] Matsumoto D,Yamane H,Abe K,et al.Identification of a Skp1-Like Protein interacting with SFB,the Pollen S determinant of the gametophytic self-incompatibility in Prunus[J].Plant Physiology,2012,159(3):1 252-1 262.
[10] YUAN Hui,MENG Dong,GU Zhaoyu,et al.A novel gene,MdSSK1,as a component of the SCF complex rather than MdSBP1 can mediate the ubiquitination of S-RNase in apple[J].Journal of Experimental Botany,2014,65(12):3 121-3 131.
[11] Wu Junkai,Li Maofu,Li Tianzhong.Genetic features of the spontaneous self-compatible mutant,'JinZhui' (Pyrus bretschneideri Rehd.)[J].PLoS One,2013,8(10):e76509.
[12] Li M F,Li X F,Han Z H,et al.Molecular analysis of two Chinese pear (Pyrus bretschneideri Rehd.) spontaneous self-compatible mutants,Yan Zhuang and Jin Zhui[J].Plant Biology(Stuttg),2009,11(5):774-783.
[13] Bradford,M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72:248-254.
[14] Goldraij A,Kondo K,Lee C B,et al.Compartmentalization of S-RNase and HT-B degradation in self-incompatible Nicotiana[J].Nature,2006,439(7 078):805-810.
[15] Luu D T,Qin X,Morse D,et al.S-RNase uptake by compatible pollen tubes in gametophytic self-incompatibility[J].Nature,2000,407(6 804):649-651.
[16] Franklintong V E.Signaling and the Modulation of Pollen Tube Growth[J].The Plant Cell,1999,11(4):727-738.
[17] Sekere? J,Pejchar P,?antru?ek J,et al.Analysis of Exocyst Subunit EXO70 Family Reveals Distinct Membrane Polar Domains in Tobacco Pollen Tubes[J].Plant Physiology,2017,173(3):1 659-1 675.
[18] ?ársky V,Kulich I,Fendrych M,et al.Exocyst complexes multiple functions in plant cells secretory pathways[J].Plant Biology,2013,16:726-733.
[19] Linton K J,Higgins C F.The Escherichia coli ATP-binding cassette (ABC) proteins[J].Molecular Microbiology,1998,28(1):5-13.
[20] Kang J,Hwang J U,Lee M,et al.PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid[J].Proc Natl Acad Sci,2010,107(5):2 355-2 360.
[21] Geisler M.Plant ABC Transporters[M].Switzerland:Springer International Publishing,2014.
[22] Theodoulou F L.Plant ABC transporters[J].Biochimica Et Biophysica Acta Biomembranes,2000,1 465(1/2):79-103.
[23] Williams J S,Der J P,dePamphilis C W,et al.Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflate[J].Plant Cell,2014,26(7):2 873-2 888.
[24] Meng Dong,Gu Zhaoyu,Li Wei,et al.Apple MdABCF assists the transportation of S-RNase into pollen tubes[J].The Plant Journal,2014,78(6):990-1 002.
[25] Choi Hyunju,Jin Junyoung,Choi Setbyoul,et al.An ABCG/WBC-type ABC transporter is essential for transport of sporopollenin precursors for exine formation in developing pollen[J].Plant Journal,2011,65(2):181-193.
[26] Cecchetti V,Brunetti P,Napoli N,et al.ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development[J].Journal of Integrative Plant Biology,2015,57(12):1 089-1 098.
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