稀土元素对短叶对齿藓生理生化的研究
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摘要
通过测定分析3个主要生理指标过氧化物酶(POD)活性、丙二醛(MDA)含量和叶绿素含量的变化,研究了短叶对齿藓组培苗在6个不同浓度梯度的轻稀土La~(3+),Ce~(4+)和重稀土Y~(3+)单一元素胁迫下的生理响应和变化。结果如下:(1)短叶对齿藓体内的过氧化物酶(POD)活性和丙二醛(MDA)含量各处理组均低于对照组; Ce~(4+)元素在3. 55×10~(-2)mmol·L~(-1)时显著提高了POD活性,在7. 1×10~(-2)mmol·L~(-1)时显著增加了膜脂过氧化产物丙二醛(MDA)含量。表明短叶对齿藓对Ce4+元素胁迫响应较强;而La~(3+)元素各处理浓度间POD的变化较为平缓,胁迫响应较弱; Y~(3+)处理居中。(2)较低浓度的La~(3+)(1. 8×10~(-2)mmol·L~(-1))和Ce~(4+)(3. 55×10~(-2)mmol·L~(-1))时显著提高叶绿素a、叶绿素b和总叶绿素含量; 3种稀土元素在较高浓度时叶绿素的含量均有明显下降。本研究为进一步探明白云鄂博稀土矿区的苔藓植物生长发育受稀土元素的影响奠定了基础。
Based on the successful application of tissue cultured seedlings of Didymodon tectorum,three physiological indexes,including POD vitality,MDA and chlorophyll content were determined to check out the influences of the rare earth elements stress under gradient concentrations treatment. We choosed the light rare earth La~(3+) ,Ce~(4+) and heavy rare earth Y~(3+) three elements and setted respectively six concentrations under the single elemnet stress,in order to explore the physiological response and changes of D. tectorum to the rare earth stress. The results were the following:( 1) The peroxidase( POD) activity and malondialdehyde( MDA) content in D. tectorum were lower in each treatment group than those in the control group; Ce~(4+) element significantly promoted the POD activity at 3. 55 × 10~(-2) mmol · L~(-1),at 7. 1 × 10~(-2) mmol·L~(-1),the content of MDA increased. It showed that the D. tectorum had a stronger response to Ce~(4+) stress,while La~(3+) showed a more gradual change in POD activity,and the stress response was weaker; Y~(3+) treatment was intermediate;( 2) At lower concentrations of La~(3+) ( 1. 8 × 10~(-2) mmol · L~(-1)) and Ce~(4+) ( 3. 55 × 10~(-2) mmol · L~(-1)) the contents of chlorophyll a,chlorophyll b and total chlorophyll increased significantly,but at higher concentrations of all three rare earth elements,the contents of chlorophyll decreased obviousily. This study laid the foundation for further exploration of the effects of rare earth elements on the growth and the development of bryophytes in theBaiyun obo rare earth mineral area.
Based on the successful application of tissue cultured seedlings of Didymodon tectorum,three physiological indexes,including POD vitality,MDA and chlorophyll content were determined to check out the influences of the rare earth elements stress under gradient concentrations treatment. We choosed the light rare earth La~(3+) ,Ce~(4+) and heavy rare earth Y~(3+) three elements and setted respectively six concentrations under the single elemnet stress,in order to explore the physiological response and changes of D. tectorum to the rare earth stress. The results were the following:( 1) The peroxidase( POD) activity and malondialdehyde( MDA) content in D. tectorum were lower in each treatment group than those in the control group; Ce~(4+) element significantly promoted the POD activity at 3. 55 × 10~(-2) mmol · L~(-1),at 7. 1 × 10~(-2) mmol·L~(-1),the content of MDA increased. It showed that the D. tectorum had a stronger response to Ce~(4+) stress,while La~(3+) showed a more gradual change in POD activity,and the stress response was weaker; Y~(3+) treatment was intermediate;( 2) At lower concentrations of La~(3+) ( 1. 8 × 10~(-2) mmol · L~(-1)) and Ce~(4+) ( 3. 55 × 10~(-2) mmol · L~(-1)) the contents of chlorophyll a,chlorophyll b and total chlorophyll increased significantly,but at higher concentrations of all three rare earth elements,the contents of chlorophyll decreased obviousily. This study laid the foundation for further exploration of the effects of rare earth elements on the growth and the development of bryophytes in theBaiyun obo rare earth mineral area.
引文
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11. Zhao X D. Study on the relationship between the habitat of Huperzia serrata and seasonal fluctuating accumulation of Huper zine A[Dissertation]. Hefei:Hefei University of Technology,2006:53.
12.王建安,沙莎,李艳芝,等. Ce(NO3)3对盾叶薯蓣茎段愈伤组织生长和不定根诱导影响的研究[J].南京师大学报:自然科学版,2010,33(1):94-97.Wang J A,Sha S,Li Y Z,et al. Effect of Ce(NO3)3on Callus Growth and Adventitious Root of Stem From Dioscorea zingberensis[J]. Journal of Nanjing Normal University:Nature Science Edition,2010,33(1):94-97.
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14.高叶青,丁彩琴,任冬梅,等.稀土元素富集对白云鄂博矿区8种常见藓类植物生长及其解剖结构特征的影响[J].西北植物学报,2017,37(1):23-31.Gao Y Q,Ding C Q,Ren D M,et al. Study on the Impacts of REE’s Enrichment on Growth and Anatomy of Eight Common Mosses from Baiyun Obo[J]. Acta Botanica Boreali-Occidentalia Sinica,2017,37(1):23-31.
15.王和勇,罗恒,孙敏.诱导子在药用植物细胞培养中的应用[J].中草药,2004,35(8):959-963.Wang H Y,Luo H,Sun M. Application of elicitor to cell culture of medicinal plants[J]. Chinese Traditional&Herbal Drugs,2004; 35(8):959-963.
16. Fiket ■,Medunic G,Turk F M,et al. Influence of soil characteristics on rare earth fingerprints in mosses and mushrooms:Example of a pristine temperate rainforest(Slavonia,Croatia).[J]. Chemosphere,2017,179:92-100.
17. Krmar M,Mihailovic D T,Arsenic I.,et al. Beryllium-7and 210pb atmospheric deposition measured in moss and dependence on cumulative precipitation[J]. Science of the Total Environment,2016. 541:941-948.
18. Rühling ?,Tyler G. Changes in the atmospheric deposition of minor and rare elements between 1975 and 2000 in south Sweden,as measured by moss analysis[J]. Environmental Pollution,2004,131(3):417-423.
2.李若愚,李强,陈胜等.包头白云鄂博采矿区周边表层土壤中232Th的分布特征[J].环境科学研究,2014,27(1):51-56.Li R Y,Li Q,Chen S,et al. Distribution characteristics of 232 Th in surface soil in the surface of Baotou Baiyunbo mining area[J]. Research of Environmental Science,2014,27(1):51-56.
3. El-Ramady H R H. A contribution on the bio-actions of rare earth elements in the soil/plant environment[D]. Carolo:der Technischen Universitt Carolo,2008.
4. Berg T,Steinnes E. Use of mosses(Hylocomium splendens and Pleurozium schreberi)as biomonitors of heavy metal deposition:from relative to absolute deposition values[J].Environmental Pollution,1997,98(1):61-71.
5. Chiarenzelli J,Aspler L,Dunn C,et al. Multi-element and rare earth element composition of lichens,mosses,and vascular plants from the Central Barrenlands,Nunavut,Canada[J]. Applied Geochemistry,2001,16(2):245-270.
6. Dolegowska S,Migaszewski Z M. Anomalous concentrations of rare earth elements in the moss-soil system from southcentral Poland[J]. Environmental Pollution,2013,178(1):33-40.
7. Babula P,Adam V,Opatrilova R,et al. Uncommon heavy metals,metalloids and their plant toxicity:a review[J].Environmental Chemistry Letters,2008,6(4):189-213.
8. Al-Khashman O A,Shawabkeh R A. Metals distribution in soils around the cement factory in southern Jordan[J]. Environmental Pollution,2006,140(3):387-394.
9.何跃君,薛立.稀土元素对植物的生物效应及其作用机理[J].应用生态学报,2005,16(10):1983-1989.He Y J,Xue L. Biological effects of rare earth elements and their action mechanisms[J]. Chinese Journal of Applied Ecology,2005,16(10):1983-1989.
10.宋卫平,洪法水,万志刚,等.镧、铕对白沙枇杷试管苗生根效应的研究[J].中国稀土学报,2002,20(5):458-462.Song W P,Hong F S,Wan Z G,et al. Effects of Lanthanum and Europium Elements on Rooting of Plantlet Loquat in vitro[J]. Journal of the Chinese Rare Earth Society,2002,20(5):458-462.
11. Zhao X D. Study on the relationship between the habitat of Huperzia serrata and seasonal fluctuating accumulation of Huper zine A[Dissertation]. Hefei:Hefei University of Technology,2006:53.
12.王建安,沙莎,李艳芝,等. Ce(NO3)3对盾叶薯蓣茎段愈伤组织生长和不定根诱导影响的研究[J].南京师大学报:自然科学版,2010,33(1):94-97.Wang J A,Sha S,Li Y Z,et al. Effect of Ce(NO3)3on Callus Growth and Adventitious Root of Stem From Dioscorea zingberensis[J]. Journal of Nanjing Normal University:Nature Science Edition,2010,33(1):94-97.
13.谢寅峰,李群,沈惠娟,等.稀土对银杏苗木叶内含物及其产量的效应[J].南京林业大学学报,2000,24(6):71-74.Xie Y F,Li Q,Shen H J,et al. Effects of rare earth elements on some leaf inclusion and leaf yield in seedlings of Ginkgo biloba[J]. Journal of Nanjing Forestry Unversity,2000,24(6):71-74.
14.高叶青,丁彩琴,任冬梅,等.稀土元素富集对白云鄂博矿区8种常见藓类植物生长及其解剖结构特征的影响[J].西北植物学报,2017,37(1):23-31.Gao Y Q,Ding C Q,Ren D M,et al. Study on the Impacts of REE’s Enrichment on Growth and Anatomy of Eight Common Mosses from Baiyun Obo[J]. Acta Botanica Boreali-Occidentalia Sinica,2017,37(1):23-31.
15.王和勇,罗恒,孙敏.诱导子在药用植物细胞培养中的应用[J].中草药,2004,35(8):959-963.Wang H Y,Luo H,Sun M. Application of elicitor to cell culture of medicinal plants[J]. Chinese Traditional&Herbal Drugs,2004; 35(8):959-963.
16. Fiket ■,Medunic G,Turk F M,et al. Influence of soil characteristics on rare earth fingerprints in mosses and mushrooms:Example of a pristine temperate rainforest(Slavonia,Croatia).[J]. Chemosphere,2017,179:92-100.
17. Krmar M,Mihailovic D T,Arsenic I.,et al. Beryllium-7and 210pb atmospheric deposition measured in moss and dependence on cumulative precipitation[J]. Science of the Total Environment,2016. 541:941-948.
18. Rühling ?,Tyler G. Changes in the atmospheric deposition of minor and rare elements between 1975 and 2000 in south Sweden,as measured by moss analysis[J]. Environmental Pollution,2004,131(3):417-423.