MALAT1基因在食管癌组织中的表达及其与肿瘤病情发展的关系
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摘要
目的:探讨MALAT1基因在食管癌组织中的表达及其与肿瘤病情发展的关系。方法:选取在本院接受食管癌根治术治疗的原发性食管癌患者98例作为研究对象,根据术中留取组织标本不同分为食管癌组织、癌旁组织两类。对比不同食管组织中MALAT1基因、食管癌增殖相关基因表达量、食管癌侵袭相关基因表达量的差异,进一步采用相关性分析评估食管癌组织中MALAT1基因表达量与肿瘤病情的内在联系。结果:食管癌组织中MALAT1 mRNA的表达量高于癌旁组织;增殖相关基因FOXA1、MACC1、MAGE-A1、Nucleostemin、ABCE1mRNA的表达量高于癌旁组织,KLF4mRNA的表达量低于癌旁组织;侵袭相关基因PAR-2、PEBP4、UHRF1、Nestin mRNA的表达量高于癌旁组织,TSLC1mRNA的表达量低于癌旁组织(P<0.05)。Pearson检验发现,食管癌组织中MALAT1基因表达量与癌细胞的增殖、侵袭活性均直接相关。结论:食管癌组织中MALAT1基因表达量异常增高,随该基因表达量增加、癌细胞的增殖及侵袭活力增加。
Objective:To investigate the expression of MALAT1 gene in esophageal carcinoma and its relationship with tumor progression.Methods:98 patients with primary esophageal cancer who underwent radical operation for esophageal cancer in our hospital between August 2015 and December 2017 were selected as the research subjects,and the tissue specimens obtained during operation were divided into esophageal cancer tissues and tissues adjacent to carcinoma.The differences in the expression of MALAT1 gene,esophageal cancer proliferation-related genes and esophageal cancer invasion-related genes in different esophageal cancer tissues were compared,and correlation analysis was used to further evaluate the inner link between MALAT1 gene expression in esophageal cancer tissues and tumor condition.Results:MALAT1 mRNA expression in esophageal cancer tissues was higher than that in adjacent tissues;proliferation-related genes FOXA1,MACC1,MAGE-A1,Nucleostemin and ABCE1 mRNA expression were higher than those in adjacent tissues whereas KLF4 mRNA expression was lower than that in adjacent tissues;invasion-related genes PAR-2,PEBP4,UHRF1 and Nestin mRNA expression were higher than those in adjacent tissues whereas TSLC1 mRNA expression was lower than that in adjacent tissues(P<0.05).Pearson test showed that the MALAT1 gene expression in esophageal cancer was directly correlated with the proliferation and invasion activity of cancer cells.Conclusion:The expression of MALAT1 gene abnormally increases in esophageal cancer tissues,and the proliferation and invasion activity of cancer cells increase with the increase of the gene expression.
Objective:To investigate the expression of MALAT1 gene in esophageal carcinoma and its relationship with tumor progression.Methods:98 patients with primary esophageal cancer who underwent radical operation for esophageal cancer in our hospital between August 2015 and December 2017 were selected as the research subjects,and the tissue specimens obtained during operation were divided into esophageal cancer tissues and tissues adjacent to carcinoma.The differences in the expression of MALAT1 gene,esophageal cancer proliferation-related genes and esophageal cancer invasion-related genes in different esophageal cancer tissues were compared,and correlation analysis was used to further evaluate the inner link between MALAT1 gene expression in esophageal cancer tissues and tumor condition.Results:MALAT1 mRNA expression in esophageal cancer tissues was higher than that in adjacent tissues;proliferation-related genes FOXA1,MACC1,MAGE-A1,Nucleostemin and ABCE1 mRNA expression were higher than those in adjacent tissues whereas KLF4 mRNA expression was lower than that in adjacent tissues;invasion-related genes PAR-2,PEBP4,UHRF1 and Nestin mRNA expression were higher than those in adjacent tissues whereas TSLC1 mRNA expression was lower than that in adjacent tissues(P<0.05).Pearson test showed that the MALAT1 gene expression in esophageal cancer was directly correlated with the proliferation and invasion activity of cancer cells.Conclusion:The expression of MALAT1 gene abnormally increases in esophageal cancer tissues,and the proliferation and invasion activity of cancer cells increase with the increase of the gene expression.
引文
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22 Han G,Zhang L,Ni X,et al.MicroRNA-873Promotes Cell Proliferation,Migration,and Invasion by Directly Targeting TSLC1in Hepatocellular Carcinoma[J].Cell Physiol Biochem,2018,46(6):2261-2270.
23 Pujari R,Hunte R,Thomas R,et al.Human T-cell leukemia virus type 1(HTLV-1)tax requires CADM1/TSLC1for inactivation of the NF-κB inhibitor A20and constitutive NF-κB signaling[J].PLoS Pathog,2015,11(3):e1004721.
2 Mota FC,Cecconello I,Takeda FR,et al.Neoadjuvant therapy or upfront surgery?A systematic review and meta-analysis of T2N0esophageal cancer treatment options[J].Int J Surg,2018,54(Pt A):176-181.
3 Amodio N,Raimondi L,Juli G,et al.MALAT1:a druggable long non-coding RNA for targeted anti-cancer approaches[J].J Hematol Oncol,2018,11(1):63.
4 Bai L,Wang A,Zhang Y,et al.Knockdown of MAL-AT1enhances chemosensitivity of ovarian cancer cells to cisplatin through inhibiting the Notch1signaling pathway[J].Exp Cell Res,2018,366(2):161-171.
5 Xiping Z,Bo C,Shifeng Y,et al.Roles of MALAT1in development and migration of triple negative and Her-2positive breast cancer[J].Oncotarget,2017,9(2):2255-2267.
6 Xiong Y,Wang J,Zhu H,et al.Chronic oxymatrine treatment induces resistance and epithelial mesenchymal transition through targeting the long non-coding RNAMALAT1in colorectal cancer cells[J].Oncol Rep,2018,39(3):967-976.
7 Li J,Zhang S,Zhu L,et al.Role of transcription factor FOXA1in non small cell lung cancer[J].Mol Med Rep,2018,17(1):509-521.
8 Zhang S,Liu Q,Zhang Q,et al.MicroRNA-30a-5p suppresses proliferation,invasion and tumor growth of hepatocellular cancer cells via targeting FOXA1[J].Oncol Lett,2017,14(4):5018-5026.
9 Ozturk E,Aksoy SA,Ugras N,et al.Coexistence of MACC1and NM23-H1dysregulation and tumor budding promise early prognostic evidence for recurrence risk of early-stage colon cancer[J].APMIS,2018,126(2):99-108.
10 Prguda-Mujic J,Milde-Langosch K,Mueller V,et al.The Predictive Significance of Metastasis-Associated in Colon Cancer-1(MACC1)in Primary Breast Cancer[J].Ann Clin Lab Sci,2018,48(2):191-196.
11 Yi E,Chang JE,Leem C,et al.Association of MAGEA1-6Expression with Lung Cancer Progression[J].JCancer,2017,8(8):1324-1329.
12 Wang J,Wang L,Ji Q,et al.Knockdown of Nucleostemin in an ovarian cancer SKOV-3cell line and its effects on cell malignancy[J].Biochem Biophys Res Commun,2017,487(2):262-267.
13 Ding X,Zhong T,Jiang L,et al.miR-25enhances cell migration and invasion in non-small-cell lung cancer cells via ERK signaling pathway by inhibiting KLF4[J].Mol Med Rep,2018,17(5):7005-7016.
14 Zheng D,Dai Y,Wang S,et al.MicroRNA-299-3p promotes the sensibility of lung cancer to doxorubicin through directly targeting ABCE1[J].Int J Clin Exp Pathol,2015,8(9):10072-10081.
15 Tian Y,Tian X,Han X,et al.ABCE1plays an essential role in lung cancer progression and metastasis[J].Tumour Biol,2016,37(6):8375-8382.
16 Chen J,Xie L,Zheng Y,et al.Effects of silenced PAR-2on cell proliferation,invasion and metastasis of esophageal cancer[J].Oncol Lett,2017,14(4):4115-4121.
17 Wu Z,Liu B,Zheng X,et al.Role of the PEBP4protein in the development and metastasis of gastric cancer[J].Oncotarget,2017,8(11):18177-18184.
18 Zhang D,Dai Y,Cai Y,et al.PEBP4promoted the growth and migration of cancer cells in pancreatic ductal adenocarcinoma[J].Tumour Biol,2016,37(2):1699-1705.
19 Yuan B,Liu Y,Yu X,et al.FOXM1contributes to taxane resistance by regulating UHRF1-controlled cancer cell stemness[J].Cell Death Dis,2018,9(5):562.
20 Hong JH,Jin EH,Kim S,et al.LINE-1hypomethylation is inversely correlated with UHRF1overexpression in gastric cancer[J].Oncol Lett,2018,15(5):6666-6670.
21 Czekierdowski A,Stachowicz N,Czekierdowska S,et al.Prognostic significance of TEM7and nestin expression in women with advanced high grade serous ovarian cancer[J].Ginekol Pol,2018,89(3):135-141.
22 Han G,Zhang L,Ni X,et al.MicroRNA-873Promotes Cell Proliferation,Migration,and Invasion by Directly Targeting TSLC1in Hepatocellular Carcinoma[J].Cell Physiol Biochem,2018,46(6):2261-2270.
23 Pujari R,Hunte R,Thomas R,et al.Human T-cell leukemia virus type 1(HTLV-1)tax requires CADM1/TSLC1for inactivation of the NF-κB inhibitor A20and constitutive NF-κB signaling[J].PLoS Pathog,2015,11(3):e1004721.