龙葵杂10号向日葵染色体核型分析
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摘要
为了探索向日葵染色体核型和染色体制片技术,以向日葵龙葵杂10号为试验材料,探索了低温预处理向日葵根尖细胞对制片效果的影响;使用Photoshop和Excel软件辅助分析染色体图像进行核型分析。结果表明:低温预处理向日葵根尖细胞,可以获得较理想的染色体制片用于核型分析,龙葵杂10号染色体数量为2n=34,核型公式为2n=34=30m+2sm+2M。
In order to analyze the chromosome karyotype and chromosome production techniques of sunflower Longkuiza10, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using sunflower Longkuiza10 as the experimental material. Photoshop and Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of sunflower Longkuiza10 was 2n=34, and the karyotype formula was 2n=34=30m+2sm+2M.
In order to analyze the chromosome karyotype and chromosome production techniques of sunflower Longkuiza10, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using sunflower Longkuiza10 as the experimental material. Photoshop and Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of sunflower Longkuiza10 was 2n=34, and the karyotype formula was 2n=34=30m+2sm+2M.
引文
[1]刘惠荣,曾子申,利容千.向日葵(H. annuus L.)三个品种的染色体组型分析.中国油料,1984(3):26-30.
[2]崔秋华,蔚荣海,韩立军.向日葵(H. annuus L.)染色体核型分析.吉林农业大学学报,1989(2):23-25.
[3]刘海学.向日葵染色体核型及其遗传转化的研究.长春:吉林大学,2007:12-14.
[4]王立,邹庆军,程晓楠.暴马丁香的染色体核型分析.种子,2015,34(11):31-32.
[5]张雷,姚洪庆,张红.麦瓶草的染色体数目观察及核型分析.德州学院学报,2015,31(4):80-83.
[6]李梦寒,次仁白珍,安克杰,等. 2个西藏白菜型油菜地方品种的核型分析及比较.安徽农学通报,2016,22(15):21-23.
[7]丁鸿,邱东萍,陈少雄.植物染色体标本的制备和染色体核型分析研究进展.南方农业学报,2012,43(12):1958-1962.
[8]李国泰.百合科4种植物染色体的核型比较.中国林副特产,2017(6):21-25.
[9]李顺.非洲菊常见品种的染色体核型与倍性分析.华南师范大学学报(自然科学版),2016,48(6):13-16.
[10]马衣努尔姑·吐地,张延辉,秦伟,等.新疆野苹果的不同种下类型染色体核型分析.中国农业科学,2016,49(8):1540-1549.
[11]魏爱民,杜胜利,韩毅科,等.植物细胞染色体倍性鉴定方法.天津农业科学,2001,7(2):41-43.
[12]Levan A,Fredga K,Sandberg A. Nomenclature for centromeric postion on chromosomes. Hereditas,1964,52(2):201-220.
[13]Arano H. Cytological studies in subfamily(Carduoideae)of Janpan:IX. The karyotype analysis. Botanical Magazine(Tokyo),1963,76:32-39.
[14]Stebbins G L. Chromosomes and evplution in higher plants.London;Edward Aronld,1971:87-89.
[15]李懋学.几种油料植物的核型分析.西北植物学报,1987,7(4):246-251.
[16]Suhaileh A. Karyotype analysis of four varieties of H. annuus.Cytologia,1979,44:319-323.
[17]张长顺.几种农作物的核型研究.云南教育学院学报,1998,14(2):21-22.
[2]崔秋华,蔚荣海,韩立军.向日葵(H. annuus L.)染色体核型分析.吉林农业大学学报,1989(2):23-25.
[3]刘海学.向日葵染色体核型及其遗传转化的研究.长春:吉林大学,2007:12-14.
[4]王立,邹庆军,程晓楠.暴马丁香的染色体核型分析.种子,2015,34(11):31-32.
[5]张雷,姚洪庆,张红.麦瓶草的染色体数目观察及核型分析.德州学院学报,2015,31(4):80-83.
[6]李梦寒,次仁白珍,安克杰,等. 2个西藏白菜型油菜地方品种的核型分析及比较.安徽农学通报,2016,22(15):21-23.
[7]丁鸿,邱东萍,陈少雄.植物染色体标本的制备和染色体核型分析研究进展.南方农业学报,2012,43(12):1958-1962.
[8]李国泰.百合科4种植物染色体的核型比较.中国林副特产,2017(6):21-25.
[9]李顺.非洲菊常见品种的染色体核型与倍性分析.华南师范大学学报(自然科学版),2016,48(6):13-16.
[10]马衣努尔姑·吐地,张延辉,秦伟,等.新疆野苹果的不同种下类型染色体核型分析.中国农业科学,2016,49(8):1540-1549.
[11]魏爱民,杜胜利,韩毅科,等.植物细胞染色体倍性鉴定方法.天津农业科学,2001,7(2):41-43.
[12]Levan A,Fredga K,Sandberg A. Nomenclature for centromeric postion on chromosomes. Hereditas,1964,52(2):201-220.
[13]Arano H. Cytological studies in subfamily(Carduoideae)of Janpan:IX. The karyotype analysis. Botanical Magazine(Tokyo),1963,76:32-39.
[14]Stebbins G L. Chromosomes and evplution in higher plants.London;Edward Aronld,1971:87-89.
[15]李懋学.几种油料植物的核型分析.西北植物学报,1987,7(4):246-251.
[16]Suhaileh A. Karyotype analysis of four varieties of H. annuus.Cytologia,1979,44:319-323.
[17]张长顺.几种农作物的核型研究.云南教育学院学报,1998,14(2):21-22.