Kidney-Qi deficiency diagnosed by Deng's diagnosis standard was not correlated with aging based on clinical observation of 90 participants
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摘要
OBJECTIVE: To verify the Traditional Chinese Medicine(TCM) theory that kidney-Qi deficiency(KQD)is considered to be the main cause of aging using cross-sectional study.METHODS: Demographic and lifestyle characteristics of 90 healthy participants were collected with a self-administered questionnaire. KQD syndrome was diagnosed according to Deng's diagnosis standard. Creatinine-adjusted urinary 8-hydroxy-2'-deoxyguanosine(8-OH-dG) and 8-isomeric-prostaglandin2α(8-iso-PGF2α), salivary advanced oxidation protein products(AOPPs), malondialdehyde(MDA) and dehydroepiandrosterone-sulfate(DHEA-S) were selected as aging markers and measured using enzyme-linked immunosorbent assay.RESULTS: No significant differences were observed in participant characteristics between the KQD group and non-KQD(NKQD) group(P > 0.05). Levels of 8-OH-dG, 8-iso-PGF2α, AOPPs, and MDA increased with age, except for a slight decrease in8-OH-dG in the older group. The increase in8-iso-PGF2α was significant(P < 0.05). DHEA-S significantly decreased with increasing age(P < 0.01).8-OH-dG levels were higher in the KQD group compared with the NKQD group. Levels of urinary8-iso-PGF2α, salivary AOPPs, and MDA in the KQD group were lower than in the NKQD group. Salivary DHEA-S was higher in the KQD group compared with the NKQD group. However, differences between KQD group and NKQD group were not significant.CONCLUSION: The current results suggested that KQD syndrome, as diagnosed by Deng's standard,does not underlie the aging phenotype.
OBJECTIVE: To verify the Traditional Chinese Medicine(TCM) theory that kidney-Qi deficiency(KQD)is considered to be the main cause of aging using cross-sectional study.METHODS: Demographic and lifestyle characteristics of 90 healthy participants were collected with a self-administered questionnaire. KQD syndrome was diagnosed according to Deng's diagnosis standard. Creatinine-adjusted urinary 8-hydroxy-2'-deoxyguanosine(8-OH-dG) and 8-isomeric-prostaglandin2α(8-iso-PGF2α), salivary advanced oxidation protein products(AOPPs), malondialdehyde(MDA) and dehydroepiandrosterone-sulfate(DHEA-S) were selected as aging markers and measured using enzyme-linked immunosorbent assay.RESULTS: No significant differences were observed in participant characteristics between the KQD group and non-KQD(NKQD) group(P > 0.05). Levels of 8-OH-dG, 8-iso-PGF2α, AOPPs, and MDA increased with age, except for a slight decrease in8-OH-dG in the older group. The increase in8-iso-PGF2α was significant(P < 0.05). DHEA-S significantly decreased with increasing age(P < 0.01).8-OH-dG levels were higher in the KQD group compared with the NKQD group. Levels of urinary8-iso-PGF2α, salivary AOPPs, and MDA in the KQD group were lower than in the NKQD group. Salivary DHEA-S was higher in the KQD group compared with the NKQD group. However, differences between KQD group and NKQD group were not significant.CONCLUSION: The current results suggested that KQD syndrome, as diagnosed by Deng's standard,does not underlie the aging phenotype.
OBJECTIVE: To verify the Traditional Chinese Medicine(TCM) theory that kidney-Qi deficiency(KQD)is considered to be the main cause of aging using cross-sectional study.METHODS: Demographic and lifestyle characteristics of 90 healthy participants were collected with a self-administered questionnaire. KQD syndrome was diagnosed according to Deng's diagnosis standard. Creatinine-adjusted urinary 8-hydroxy-2'-deoxyguanosine(8-OH-dG) and 8-isomeric-prostaglandin2α(8-iso-PGF2α), salivary advanced oxidation protein products(AOPPs), malondialdehyde(MDA) and dehydroepiandrosterone-sulfate(DHEA-S) were selected as aging markers and measured using enzyme-linked immunosorbent assay.RESULTS: No significant differences were observed in participant characteristics between the KQD group and non-KQD(NKQD) group(P > 0.05). Levels of 8-OH-dG, 8-iso-PGF2α, AOPPs, and MDA increased with age, except for a slight decrease in8-OH-dG in the older group. The increase in8-iso-PGF2α was significant(P < 0.05). DHEA-S significantly decreased with increasing age(P < 0.01).8-OH-dG levels were higher in the KQD group compared with the NKQD group. Levels of urinary8-iso-PGF2α, salivary AOPPs, and MDA in the KQD group were lower than in the NKQD group. Salivary DHEA-S was higher in the KQD group compared with the NKQD group. However, differences between KQD group and NKQD group were not significant.CONCLUSION: The current results suggested that KQD syndrome, as diagnosed by Deng's standard,does not underlie the aging phenotype.
引文
1 Li Y,Tian Y,Li J,et al.Bufei Yishen granules combined with acupoint sticking therapy suppress oxidative stress in chronic obstructive pulmonary disease rats:via regulating peroxisome proliferator-activated receptor-gamma signaling.J Ethnopharmacol 2016;193:354-361.
2 Xu H,Lawson D,Kras A,Ryan D.The use of preventive strategies for bone loss.Am J Chin Med 2005;33(2):299-306.
3 Guo X.The basic theory of Traditional Chinese Medicine.Shanghai:Shanghai Science and Technology Press,2006:145.
4 Wang X.Textbook of Traditional Chinese Medicine.Beijing:Science Press,2016:136-137.
5 Wang S.Comparison of aging theory in Traditional Chinese Medicine and Western Medicine.Shi Zheng Guo Yi Guo Yao 2009;20(5):1274-1275.
6 Tseng YT,Chang FR,Lo YC.The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death.Phytomedicine 2014;21(5):724-733.
7 Shih HC,Chang KH,Chen FL,et al.Anti-aging effects of the Traditional Chinese Medicine bu-zhong-yi-Qi-tang in mice.Am J Chin Med 2000;28(1):77-86.
8 Tan Y,Liu X,Lu C,et al.Metabolic profiling reveals therapeutic biomarkers of processed Aconitum carmichaeli Debx in treating hydrocortisone induced kidney-Yang deficiency syndrome rats.J Ethnopharmacol 2014;152(3):585-593.
9 Shen ZY.The location of deficiency syndrome of kidney Yang.Chinese Med J-Peking 1999;112(11):973-975.
10 Shen ZY.On similarities and differences of kidney Yang deficiency and potent energy of aged patients based upon the view of internal environment adjustment.Zhong Guo Zhong Xi Yi Jie He Za Zhi 1993;13(6):362-364.
11 Tsang WP,Zhang F,He Q,et al.Icaritin enhances m ESCself-renewal through upregulating core pluripotency transcription factors mediated by ER alpha.Sci Rep-UK 2017;7(40894).
12 Zhang S,Cai W,Huang J,et al.Icariin,a natural flavonol glycoside,extends healthspan in mice.Exp Gerontol 2015;69:226-235.
13 Cai W,Huang J,Zhang S,et al.Icariin and its derivative IcarisideⅡextend healthspan via insulin/IGF-1 pathway in C.elegans.PLoS One 2011;6(e2883512).
14 Shen ZY,Yuan CY,Huang JH.The efficacy of epimedium flavonoids on senescence-delaying in drosophila and its underlying molecular mechanisms.Zhong Guo Lao Nian Xue Za Zhi 2005;9:1061-1063.
15 Deng T,Deng Z,Luo Y.Diagnosis standard of Traditional Chinese Medicine syndrome.Guangzhou:Guangdong Science and Technology Press,1990:424.
16 Barja G,Herrero A.Oxidative damage to mitochondrial DNA is inversely related to maximum life span in the heart and brain of mammals.Faseb J 2000;14(2):312-318.
17 Lopez-Otin C,Blasco MA,Partridge L,Serrano M,Kroemer G.The hallmarks of aging.Cell 2013;153(6):1194-1217.
18 Tacconelli S,Capone ML,Patrignani P.Measurement of 8-iso-prostaglandin F2alpha in biological fluids as a measure of lipid peroxidation.Methods Mol Biol 2010;644:165-178.
19 Jacob KD,Noren HN,Trzeciak AR,Evans MK.Markers of oxidant stress that are clinically relevant in aging and age-related disease.Mech Ageing Dev 2013;134(3-4):139-157.
20 Zeng Q,E LL,Wang DS,Liu HC.Relationship of advanced oxidative protein products in human saliva and plasma:age-and gender-related changes and stability during storage.Free Radic Res 2012;46(10):1201-1206.
21 Grune T.Protein oxidation products as biomarkers.Free Radic Biol Med 2014;75 Suppl 1:S7.
22 Perret M,Aujard F.Aging and season affect plasma dehydroepiandrosterone sulfate(DHEA-S)levels in a primate.Exp Gerontol 2005;40(7):582-587.
23 Khalil AM,Gagaa MH,Alshamali AM.8-Oxo-7,8-dihydro-2'-deoxyguanosine as a biomarker of DNA damage by mobile phone radiation.Hum Exp Toxicol 2012;31(7):734-740.
24 Nie B,Gan W,Shi F,et al.Age-dependent accumulation of 8-oxoguanine in the DNA and RNA in various rat tissues.Oxid Med Cell Longev 2013;2013:303181.
25 Zhang Y,Zhang L,Zhang L,Bai J,Ge H,Liu P.Expression changes in DNA repair enzymes and mitochondrial DNA damage in aging rat lens.Mol Vis 2010;16:1754-1763.
26 Wang AL,Lukas TJ,Yuan M,Neufeld AH.Increased mitochondrial DNA damage and down-regulation of DNArepair enzymes in aged rodent retinal pigment epithelium and choroid.Mol Vis 2008;14:644-651.
27 Kondo T,Ohshima T,Ishida Y.Age-dependent expression of 8-hydroxy-2'-deoxyguanosine in human pituitary gland.Histochem J 2001;33(11-12):647-651.
28 Yanar K,Aydin S,Cakatay U,et al.Protein and DNA oxidation in different anatomic regions of rat brain in a mimetic ageing model.Basic Clin Pharmacol Toxicol 2011;109(6):423-433.
29 Pilger A,Rudiger HW.8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures.Int Arch Occup Environ Health 2006;80(1):1-15.
30 Yavuzer H,Yavuzer S,Cengiz M,et al.Biomarkers of lipid peroxidation related to hypertension in aging.Hypertens Res 2016;39(5):342-348.
31 Wang H,Zhao B,Vrcek I,Johnston JM,He Y.Role of malondialdehyde in the age-related macular degeneration.In:Oxidative Stress in Applied Basic Research and Clinical Practice.New Jersey:Humana Press,2012:85-93.
32 Rea IM,McMaster D,Donnelly J,McGrath LT,Young IS.Malondialdehyde and measures of antioxidant activity in subjects from the Belfast elderly longitudinal free-living aging study.In:Annals of the New York Academy of Sciences 2004;1019:392-395.
33 Mutlu-Turkoglu U,Ilhan E,Oztezcan S,Kuru A,Aykac-Toker G,Uysal M.Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects.Clin Biochem2003;36(5):397-400.
34 Traverso N,Patriarca S,Balbis E,et al.Anti malondialdehyde-adduct immunological response as a possible marker of successful aging.Exp Gerontol 2003;38(10):1129-1135.
35 Melough MM,Sun X,Chun OK.The Role of AOPP in age-related bone loss and the potential benefits of Berry Anthocyanins.Nutrients 2017;9(7):E789.
36 Willis EL,Wolf RF,White GL,McFarlane D.Age-and gender-associated changes in the concentrations of serum TGF-1beta,DHEA-S and IGF-1 in healthy captive baboons(Papio hamadryas anubis).Gen Comp Endocrinol2014;195:21-27.
37 Edes AN.Dehydroepiandrosterone-sulfate(DHEA-S),sex,and age in zoo-housed western lowland gorillas(Gorilla gorilla gorilla).Primates 2017;58(3):385-392.
38 Buford TW,Willoughby DS.Impact of DHEA(S)and cortisol on immune function in aging:a brief review.Appl Physiol Nutr Metab 2008;33(3):429-433.
39 Zhang D,Sun L.Note and translation of Huangdi's classic on medicine.Beijing:New World Press,2008:426.
2 Xu H,Lawson D,Kras A,Ryan D.The use of preventive strategies for bone loss.Am J Chin Med 2005;33(2):299-306.
3 Guo X.The basic theory of Traditional Chinese Medicine.Shanghai:Shanghai Science and Technology Press,2006:145.
4 Wang X.Textbook of Traditional Chinese Medicine.Beijing:Science Press,2016:136-137.
5 Wang S.Comparison of aging theory in Traditional Chinese Medicine and Western Medicine.Shi Zheng Guo Yi Guo Yao 2009;20(5):1274-1275.
6 Tseng YT,Chang FR,Lo YC.The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death.Phytomedicine 2014;21(5):724-733.
7 Shih HC,Chang KH,Chen FL,et al.Anti-aging effects of the Traditional Chinese Medicine bu-zhong-yi-Qi-tang in mice.Am J Chin Med 2000;28(1):77-86.
8 Tan Y,Liu X,Lu C,et al.Metabolic profiling reveals therapeutic biomarkers of processed Aconitum carmichaeli Debx in treating hydrocortisone induced kidney-Yang deficiency syndrome rats.J Ethnopharmacol 2014;152(3):585-593.
9 Shen ZY.The location of deficiency syndrome of kidney Yang.Chinese Med J-Peking 1999;112(11):973-975.
10 Shen ZY.On similarities and differences of kidney Yang deficiency and potent energy of aged patients based upon the view of internal environment adjustment.Zhong Guo Zhong Xi Yi Jie He Za Zhi 1993;13(6):362-364.
11 Tsang WP,Zhang F,He Q,et al.Icaritin enhances m ESCself-renewal through upregulating core pluripotency transcription factors mediated by ER alpha.Sci Rep-UK 2017;7(40894).
12 Zhang S,Cai W,Huang J,et al.Icariin,a natural flavonol glycoside,extends healthspan in mice.Exp Gerontol 2015;69:226-235.
13 Cai W,Huang J,Zhang S,et al.Icariin and its derivative IcarisideⅡextend healthspan via insulin/IGF-1 pathway in C.elegans.PLoS One 2011;6(e2883512).
14 Shen ZY,Yuan CY,Huang JH.The efficacy of epimedium flavonoids on senescence-delaying in drosophila and its underlying molecular mechanisms.Zhong Guo Lao Nian Xue Za Zhi 2005;9:1061-1063.
15 Deng T,Deng Z,Luo Y.Diagnosis standard of Traditional Chinese Medicine syndrome.Guangzhou:Guangdong Science and Technology Press,1990:424.
16 Barja G,Herrero A.Oxidative damage to mitochondrial DNA is inversely related to maximum life span in the heart and brain of mammals.Faseb J 2000;14(2):312-318.
17 Lopez-Otin C,Blasco MA,Partridge L,Serrano M,Kroemer G.The hallmarks of aging.Cell 2013;153(6):1194-1217.
18 Tacconelli S,Capone ML,Patrignani P.Measurement of 8-iso-prostaglandin F2alpha in biological fluids as a measure of lipid peroxidation.Methods Mol Biol 2010;644:165-178.
19 Jacob KD,Noren HN,Trzeciak AR,Evans MK.Markers of oxidant stress that are clinically relevant in aging and age-related disease.Mech Ageing Dev 2013;134(3-4):139-157.
20 Zeng Q,E LL,Wang DS,Liu HC.Relationship of advanced oxidative protein products in human saliva and plasma:age-and gender-related changes and stability during storage.Free Radic Res 2012;46(10):1201-1206.
21 Grune T.Protein oxidation products as biomarkers.Free Radic Biol Med 2014;75 Suppl 1:S7.
22 Perret M,Aujard F.Aging and season affect plasma dehydroepiandrosterone sulfate(DHEA-S)levels in a primate.Exp Gerontol 2005;40(7):582-587.
23 Khalil AM,Gagaa MH,Alshamali AM.8-Oxo-7,8-dihydro-2'-deoxyguanosine as a biomarker of DNA damage by mobile phone radiation.Hum Exp Toxicol 2012;31(7):734-740.
24 Nie B,Gan W,Shi F,et al.Age-dependent accumulation of 8-oxoguanine in the DNA and RNA in various rat tissues.Oxid Med Cell Longev 2013;2013:303181.
25 Zhang Y,Zhang L,Zhang L,Bai J,Ge H,Liu P.Expression changes in DNA repair enzymes and mitochondrial DNA damage in aging rat lens.Mol Vis 2010;16:1754-1763.
26 Wang AL,Lukas TJ,Yuan M,Neufeld AH.Increased mitochondrial DNA damage and down-regulation of DNArepair enzymes in aged rodent retinal pigment epithelium and choroid.Mol Vis 2008;14:644-651.
27 Kondo T,Ohshima T,Ishida Y.Age-dependent expression of 8-hydroxy-2'-deoxyguanosine in human pituitary gland.Histochem J 2001;33(11-12):647-651.
28 Yanar K,Aydin S,Cakatay U,et al.Protein and DNA oxidation in different anatomic regions of rat brain in a mimetic ageing model.Basic Clin Pharmacol Toxicol 2011;109(6):423-433.
29 Pilger A,Rudiger HW.8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures.Int Arch Occup Environ Health 2006;80(1):1-15.
30 Yavuzer H,Yavuzer S,Cengiz M,et al.Biomarkers of lipid peroxidation related to hypertension in aging.Hypertens Res 2016;39(5):342-348.
31 Wang H,Zhao B,Vrcek I,Johnston JM,He Y.Role of malondialdehyde in the age-related macular degeneration.In:Oxidative Stress in Applied Basic Research and Clinical Practice.New Jersey:Humana Press,2012:85-93.
32 Rea IM,McMaster D,Donnelly J,McGrath LT,Young IS.Malondialdehyde and measures of antioxidant activity in subjects from the Belfast elderly longitudinal free-living aging study.In:Annals of the New York Academy of Sciences 2004;1019:392-395.
33 Mutlu-Turkoglu U,Ilhan E,Oztezcan S,Kuru A,Aykac-Toker G,Uysal M.Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects.Clin Biochem2003;36(5):397-400.
34 Traverso N,Patriarca S,Balbis E,et al.Anti malondialdehyde-adduct immunological response as a possible marker of successful aging.Exp Gerontol 2003;38(10):1129-1135.
35 Melough MM,Sun X,Chun OK.The Role of AOPP in age-related bone loss and the potential benefits of Berry Anthocyanins.Nutrients 2017;9(7):E789.
36 Willis EL,Wolf RF,White GL,McFarlane D.Age-and gender-associated changes in the concentrations of serum TGF-1beta,DHEA-S and IGF-1 in healthy captive baboons(Papio hamadryas anubis).Gen Comp Endocrinol2014;195:21-27.
37 Edes AN.Dehydroepiandrosterone-sulfate(DHEA-S),sex,and age in zoo-housed western lowland gorillas(Gorilla gorilla gorilla).Primates 2017;58(3):385-392.
38 Buford TW,Willoughby DS.Impact of DHEA(S)and cortisol on immune function in aging:a brief review.Appl Physiol Nutr Metab 2008;33(3):429-433.
39 Zhang D,Sun L.Note and translation of Huangdi's classic on medicine.Beijing:New World Press,2008:426.