MIF mRNA在胃窦胃癌组织中的表达及其与幽门螺杆菌感染的关系
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摘要
目的:探讨胃窦胃癌组织中人巨噬细胞移动抑制因子MIF mRNA的表达,并分析其与幽门螺杆菌感染的关系,分析二者在胃窦胃癌发生中的相关性。方法:选取2013年1月至2014年12月于我院收治的胃窦胃癌患者30例作为观察组,另随机选择10例胃窦胃炎患者作为对照组,采用14C-尿素呼气试验(UBT)检测各组患者有无幽门螺杆菌感染,定量逆转录PCR检测观察组患者及对照组患者组织中MIF mRNA表达。统计分析不同组织中MIF mRNA表达与幽门螺杆菌感染之间的关系。结果:观察组组织中MIF mRNA的表达为(1.09±0.11),高于对照组组织的(0.21±0.08),差异具有统计学意义(P<0.05)。进一步亚组分析,观察组合并幽门螺杆菌感染组织中MIF mRNA的表达为(1.24±0.14),高于非幽门螺杆菌感染者的(1.09±0.11),差异具有统计学意义(P<0.05)。结论:MIF mRNA在胃窦胃癌组织中高表达,幽门螺杆菌感染促进了MIF mRNA的表达,共同促进了胃窦胃癌的发生发展。
Objective: To investigate the human MIF mRNA expression in tissues from patients with gastric antrum cancers and to analyze its relationship with Helicobacter pylori infections in the development of gastric antrum cancer. Methods: 30 patients with gastric antrum cancer in our hospital from January 2013 to December 2014 were selected as observation group, and 10 patients with gastric antral gastritis were randomly selected as control group. 14C-urea breath test(UBT) was used to detect Helicobacter pylori infections or not in each group, and quantitative reverse transcription PCR was used to detect the levels of MIF mRNA expression in the tissues from the patients of the observation group and the control group. Statistically analysised the relationship between the expression of MIF mRNA in different tissues and Helicobacter pylori infection. Results: The level of MIF mRNA expression in tissues from observation group was(1.09±0.11), which was significantly higher in tissues from control group(0.207 ±0.08), the difference was statistically significant(P<0.05). Trough further subgroup analysis,the levels of MIF mRNA expression in Hp infection patients with gastric antrum cancer were(1.24±0.14), which were significantly higher those in non-Hp infection patients(1.09±0.11), the difference was statistically significant(P<0.05). Conclusion: MIF mRNA is highly expressed in gastric antrum carcinoma, and Helicobacter pylori infection promotes the expression of MIF mRNA, and they promote together the development of gastric antrum cancer.
Objective: To investigate the human MIF mRNA expression in tissues from patients with gastric antrum cancers and to analyze its relationship with Helicobacter pylori infections in the development of gastric antrum cancer. Methods: 30 patients with gastric antrum cancer in our hospital from January 2013 to December 2014 were selected as observation group, and 10 patients with gastric antral gastritis were randomly selected as control group. 14C-urea breath test(UBT) was used to detect Helicobacter pylori infections or not in each group, and quantitative reverse transcription PCR was used to detect the levels of MIF mRNA expression in the tissues from the patients of the observation group and the control group. Statistically analysised the relationship between the expression of MIF mRNA in different tissues and Helicobacter pylori infection. Results: The level of MIF mRNA expression in tissues from observation group was(1.09±0.11), which was significantly higher in tissues from control group(0.207 ±0.08), the difference was statistically significant(P<0.05). Trough further subgroup analysis,the levels of MIF mRNA expression in Hp infection patients with gastric antrum cancer were(1.24±0.14), which were significantly higher those in non-Hp infection patients(1.09±0.11), the difference was statistically significant(P<0.05). Conclusion: MIF mRNA is highly expressed in gastric antrum carcinoma, and Helicobacter pylori infection promotes the expression of MIF mRNA, and they promote together the development of gastric antrum cancer.
引文
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[5]李晨,王卫星,左腾,等.MIF抑制剂ISO-1在妊娠大鼠重症急性胰腺炎肺损伤中的作用[J].海南医学,2016,(2):180-183Li Chen,Wang Wei-xing,Zuo Teng,et al.Effects of MIF inhibitor ISO-1 on lung injury in rat models of acute pancreatitis in pregnancy[J].Hainan Medical Journal,2016,(2):180-183
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[10]Salem SA,Asaad MK,Elsayed SB,et al.Evaluation of macrophage migration inhibitory factor(MIF)levels in serum and lesional skin of patients with alopecia areata[J].Int J Dermatol,2016,55(12):1357-1361
[11]Bloom J,Metz C,Nalawade S,et al.Identification of Iguratimod as an Inhibitor of Macrophage Migration Inhibitory Factor(MIF)with Steroid-sparing Potential[J].J Biol Chem,2016,291(51):26502-26514
[12]Zhang Y,Gu R,Jia J,et al.Inhibition of macrophage migration inhi bitory factor(MIF)tautomerase activity suppresses microglia-mediated inflammatory responses[J].Clin Exp Pharmacol Physiol,2016,43(11):1134-1144
[13]Yin S,Huang J,Li Z,et al.The Prognostic and Clinicopathological Significance of Tumor-Associated Macrophages in Patients with Gastric Cancer:A Meta-Analysis[J].PLo S One,2017,12(1):e0170042
[14]Wang WM,Liu JC.Effect and molecular mechanism of mir-146a on proliferation of lung cancer cells by targeting and regulating MIF gene[J].Asian Pac J Trop Med,2016,9(8):806-811
[15]Yang S,He P,Wang J,et al.A Novel MIF Signaling Pathway Drives the Malignant Character of Pancreatic Cancer by Targeting NR3C2[J].Cancer Res,2016,76(13):3838-3850
[16]曾钢,李彩东,吴斌,等.乙型肝炎肝硬化患者外周血MIF、IL-17和IL-10检测的临床意义[J].国际检验医学杂志,2015,36(9):1205-1206,1209Zeng Gang,Li Cai-dong,Wu Bin,et al.Clinical significance of detecting peripheral blood MIF,IL-17 and IL-10 in patients with liver cirrhosis[J].International Journal of Laboratory Medicine,2015,36(9):1205-1206,1209
[17]Kim BS,Stoppe C,Grieb G,et al.The clinical significance of the MIF homolog d-dopachrome tautomerase(MIF-2)and its circulating receptor(s CD74)in burn[J].Burns,2016,42(6):1265-1276
[18]Fukaya R,Ohta S,Yaguchi T,et al.MIF Maintains the Tumorigenic Capacity of Brain Tumor-Initiating Cells by Directly Inhibiting p53[J].Cancer Res,2016,76(9):2813-2823
[19]Kountouras J,Polyzos SA,Zeglinas C,et al.Helicobacter pylori-related metabolic syndrome as predictor of progression to esophageal carcinoma in a subpopulation-based Barrett's esophagus cohort[J].Gastrointest Endosc,2017,85(2):462-463
[20]王建军,王泽友,姚永良,等.幽门螺杆菌作用巨噬细胞后诱导的炎症反应研究[J].中国免疫学杂志,2015,31(6):737-740Wang Jian-jun,Wang Ze-you,Yao Yong-liang,et al.Research on inflammatory response of Helicobacter pylori-treated macrophages[J].Chinese Journal of Immunology,2015,31(6):737-740
[21]王巍,潘志峰,唐伟跃,等.胃癌患者血红蛋白的表面增强拉曼光谱分析[J].光谱学与光谱分析,2015,35(12):3402-3405Wang Wei,Pan Zhi-feng,Tang Wei-yue,et al.Research on Early Diagnosis of Gastric Cancer by the Surface Enhanced Raman Spectroscopy of Human Hemoglobin[J].Spectroscopy and Spectral Analysis,2015,35(12):3402-3405
[22]Tustumi F,Bernardo WM,Dias AR,et al.Detection value of free cancer cells in peritoneal washing in gastric cancer:a systematic review and meta-analysis[J].Clinics(Sao Paulo),2016,71(12):733-745
[23]Ahn JS,Sinn DH,Son HJ,et al.Letter:Helicobacter pylori-related non-alcoholic fatty liver disease with concomitant metabolic syndrome as risk factor for colorectal neoplasia-authors'reply[J].Aliment Pharmacol Ther,2017,45(4):577-578
[24]Graham A,Falcone T,Nothnick WB.The expression of micro RNA-451 in human endometriotic lesions is inversely related to that of macrophage migration inhibitory factor(MIF)and regulates MIF expression and modulation of epithelial cell survival[J].Hum Reprod,2015,30(3):642-652
[25]刘展艳,赵子潇,易纯,等.mi R-451下调MIF的表达并抑制其促鼻咽癌细胞浸润和转移的作用[J].现代肿瘤医学,2016,24(4):527-530Liu Zhan-yan,Zhao Zi-xiao,Yi Chun,et al.mi R-451 downregulates the expression of macrophage migration inhibitory factor(MIF)and inhibits the effection that MIF promotes the invasion and metastasis in nasopharyngeal carcinoma cells[J].Journal of Modern Oncology,2016,24(4):527-530
[26]Zeiner PS,Preusse C,Blank AE,et al.MIF Receptor CD74 is Restricted to Microglia/Macrophages,Associated with a M1-Polarized Immune Milieu and Prolonged Patient Survival in Gliomas[J].Brain Pathol,2015,25(4):491-504
[2]密玉帅,岑刚,张威浩,等.长链非编码RNA在胃癌中的研究进展[J].现代生物医学进展,2016,16(13):2548-2551Mi Yu-shuai,Cen Gang,Zhang Wei-hao,et al.The Research Progress of lnc RNAs in Gastric Cancer[J].Progress in Modern Biomedicine,2016,16(13):2548-2551
[3]Shahi H,Bahreiny R,Reiisi S.Helicobacter pylori and Its Virulence Factors'Effect on Serum Oxidative DNA Damages in Adults With Dyspepsia[J].Acta Med Iran,2016,54(11):704-708
[4]Tsujimae M,Yamashita H,Hashimura H,et al.A Comparative Study of a New Class of Gastric Acid Suppressant Agent Named Vonoparazan versus Esomeprazole for the Eradication of Helicobacter pylori[J].Digestion,2016,94(4):240-246
[5]李晨,王卫星,左腾,等.MIF抑制剂ISO-1在妊娠大鼠重症急性胰腺炎肺损伤中的作用[J].海南医学,2016,(2):180-183Li Chen,Wang Wei-xing,Zuo Teng,et al.Effects of MIF inhibitor ISO-1 on lung injury in rat models of acute pancreatitis in pregnancy[J].Hainan Medical Journal,2016,(2):180-183
[6]Ohta S,Yaguchi T,Okuno H,et al.CHD7 promotes proliferation of neural stem cells mediated by MIF[J].Mol Brain,2016,9(1):96
[7]赵光磊,谭辉,田茂松,等.食管鳞癌组织中巨噬细胞移动抑制因子、金属蛋白酶-9和血管内皮生长因子的表达意义[J].中国老年学杂志,2015,35(24):7096-7098Zhao Guang-lei,Tan Hui,Tian Mao-song,et al.Expression and significance of macrophage migration inhibitory factor,matrix metalloproteinase-9 and vascular endothelial growth factor in esophageal squamous cell carcinoma[J].Chinese Journal of Gerontology,2015,35(24):7096-7098
[8]赵夏平,李晓云,于晓辉,等.MIF、IL-17、IL-10在肝细胞癌组织中的表达及临床意义[J].临床肿瘤学杂志,2014,19(4):307-311Zhao Xia-ping,Li Xiao-yun,Yu Xiao-hui,et al.Expression of MIF,IL-17 and IL-10 in hepatocellular carcinoma and their clinical significance[J].Chinese Clinical Oncology,2014,19(4):307-311
[9]Yoo SA,Leng L,Kim BJ,et al.MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis[J].Proc Natl Acad Sci U S A,2016,113(49):E7917-E7926
[10]Salem SA,Asaad MK,Elsayed SB,et al.Evaluation of macrophage migration inhibitory factor(MIF)levels in serum and lesional skin of patients with alopecia areata[J].Int J Dermatol,2016,55(12):1357-1361
[11]Bloom J,Metz C,Nalawade S,et al.Identification of Iguratimod as an Inhibitor of Macrophage Migration Inhibitory Factor(MIF)with Steroid-sparing Potential[J].J Biol Chem,2016,291(51):26502-26514
[12]Zhang Y,Gu R,Jia J,et al.Inhibition of macrophage migration inhi bitory factor(MIF)tautomerase activity suppresses microglia-mediated inflammatory responses[J].Clin Exp Pharmacol Physiol,2016,43(11):1134-1144
[13]Yin S,Huang J,Li Z,et al.The Prognostic and Clinicopathological Significance of Tumor-Associated Macrophages in Patients with Gastric Cancer:A Meta-Analysis[J].PLo S One,2017,12(1):e0170042
[14]Wang WM,Liu JC.Effect and molecular mechanism of mir-146a on proliferation of lung cancer cells by targeting and regulating MIF gene[J].Asian Pac J Trop Med,2016,9(8):806-811
[15]Yang S,He P,Wang J,et al.A Novel MIF Signaling Pathway Drives the Malignant Character of Pancreatic Cancer by Targeting NR3C2[J].Cancer Res,2016,76(13):3838-3850
[16]曾钢,李彩东,吴斌,等.乙型肝炎肝硬化患者外周血MIF、IL-17和IL-10检测的临床意义[J].国际检验医学杂志,2015,36(9):1205-1206,1209Zeng Gang,Li Cai-dong,Wu Bin,et al.Clinical significance of detecting peripheral blood MIF,IL-17 and IL-10 in patients with liver cirrhosis[J].International Journal of Laboratory Medicine,2015,36(9):1205-1206,1209
[17]Kim BS,Stoppe C,Grieb G,et al.The clinical significance of the MIF homolog d-dopachrome tautomerase(MIF-2)and its circulating receptor(s CD74)in burn[J].Burns,2016,42(6):1265-1276
[18]Fukaya R,Ohta S,Yaguchi T,et al.MIF Maintains the Tumorigenic Capacity of Brain Tumor-Initiating Cells by Directly Inhibiting p53[J].Cancer Res,2016,76(9):2813-2823
[19]Kountouras J,Polyzos SA,Zeglinas C,et al.Helicobacter pylori-related metabolic syndrome as predictor of progression to esophageal carcinoma in a subpopulation-based Barrett's esophagus cohort[J].Gastrointest Endosc,2017,85(2):462-463
[20]王建军,王泽友,姚永良,等.幽门螺杆菌作用巨噬细胞后诱导的炎症反应研究[J].中国免疫学杂志,2015,31(6):737-740Wang Jian-jun,Wang Ze-you,Yao Yong-liang,et al.Research on inflammatory response of Helicobacter pylori-treated macrophages[J].Chinese Journal of Immunology,2015,31(6):737-740
[21]王巍,潘志峰,唐伟跃,等.胃癌患者血红蛋白的表面增强拉曼光谱分析[J].光谱学与光谱分析,2015,35(12):3402-3405Wang Wei,Pan Zhi-feng,Tang Wei-yue,et al.Research on Early Diagnosis of Gastric Cancer by the Surface Enhanced Raman Spectroscopy of Human Hemoglobin[J].Spectroscopy and Spectral Analysis,2015,35(12):3402-3405
[22]Tustumi F,Bernardo WM,Dias AR,et al.Detection value of free cancer cells in peritoneal washing in gastric cancer:a systematic review and meta-analysis[J].Clinics(Sao Paulo),2016,71(12):733-745
[23]Ahn JS,Sinn DH,Son HJ,et al.Letter:Helicobacter pylori-related non-alcoholic fatty liver disease with concomitant metabolic syndrome as risk factor for colorectal neoplasia-authors'reply[J].Aliment Pharmacol Ther,2017,45(4):577-578
[24]Graham A,Falcone T,Nothnick WB.The expression of micro RNA-451 in human endometriotic lesions is inversely related to that of macrophage migration inhibitory factor(MIF)and regulates MIF expression and modulation of epithelial cell survival[J].Hum Reprod,2015,30(3):642-652
[25]刘展艳,赵子潇,易纯,等.mi R-451下调MIF的表达并抑制其促鼻咽癌细胞浸润和转移的作用[J].现代肿瘤医学,2016,24(4):527-530Liu Zhan-yan,Zhao Zi-xiao,Yi Chun,et al.mi R-451 downregulates the expression of macrophage migration inhibitory factor(MIF)and inhibits the effection that MIF promotes the invasion and metastasis in nasopharyngeal carcinoma cells[J].Journal of Modern Oncology,2016,24(4):527-530
[26]Zeiner PS,Preusse C,Blank AE,et al.MIF Receptor CD74 is Restricted to Microglia/Macrophages,Associated with a M1-Polarized Immune Milieu and Prolonged Patient Survival in Gliomas[J].Brain Pathol,2015,25(4):491-504