Linc-OIP5调控乳腺癌细胞的增殖和侵袭
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摘要
目的本研究旨在探讨linc-OIP5与乳腺癌恶性程度的关系,以及对乳腺癌细胞生物学功能的影响。方法采用RT-PCR方法检测linc-OIP5在不同恶性程度的乳腺癌细胞系中的表达情况;采用转染si RNA方法干扰乳腺癌细胞中的linc-OIP5基因,后以RT-PCR方法检测干扰效率;采用CCK8方法检测乳腺癌细胞的增殖能力;以及Transwell细胞侵袭实验检测细胞的侵袭能力。结果乳腺癌细胞中linc-OIP5的表达量与细胞的恶性程度呈正相关。在细胞功能实验中,与对照组相比,linc-OIP5干扰组细胞的增殖和侵袭能力均减弱。结论 Linc-OIP5在乳腺癌中起到致癌基因的作用,可以调控乳腺癌细胞的增殖和侵袭能力。
Objective To investigate the relationship between linc-OIP5 and the malignancy of breast cancer,and the effect of linc-OIP5 on the biological function of breast cancer cells. Methods RT-PCR was used to detect the expression of linc-OIP5 in breast cancer cell lines with different malignant degrees. The linc-OIP5 in breast cancer cells was knocked down by si RNA,and the interference efficiency was detected by RT-PCR. The proliferation activity of breast cancer cells was detected by CCK8 assay,and the invasion ability was measured by Transwell cell invasion assay. Results The relative expression of linc-OIP5 was positively correlated with the malignant degree of breast cancer cells. In the cell function experiment,the proliferation and invasiveness of the cells in the linc-OIP5 knockdown group were decreased compared with the control group. Conclusion Linc-OIP5 plays an oncogenic role in breast cancer and could regulate the proliferation and invasiveness of breast cancer cells.
Objective To investigate the relationship between linc-OIP5 and the malignancy of breast cancer,and the effect of linc-OIP5 on the biological function of breast cancer cells. Methods RT-PCR was used to detect the expression of linc-OIP5 in breast cancer cell lines with different malignant degrees. The linc-OIP5 in breast cancer cells was knocked down by si RNA,and the interference efficiency was detected by RT-PCR. The proliferation activity of breast cancer cells was detected by CCK8 assay,and the invasion ability was measured by Transwell cell invasion assay. Results The relative expression of linc-OIP5 was positively correlated with the malignant degree of breast cancer cells. In the cell function experiment,the proliferation and invasiveness of the cells in the linc-OIP5 knockdown group were decreased compared with the control group. Conclusion Linc-OIP5 plays an oncogenic role in breast cancer and could regulate the proliferation and invasiveness of breast cancer cells.
引文
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[2]ZHAO W Y,GENG D H,LI S Q,et al.LncRNA HOTAIR influences cell growth,migration,invasion,and apoptosis via the mi R-20a-5p/HMGA2 axis in breast cancer[J].Cancer Medicine,2018,7(3):842-55.
[3]FAN S,FAN C N,LIU N,et al.Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation,migration and invasion in breast cancer[J].Molecular Medicine Reports,2018,17(8):6405-12.
[4]XU Y L,LIN X Y,XU J W,et al.SULT1E1 inhibits cell proliferation and invasion by activating PPARγin breast cancer[J].Journal of Cancer,2018,9(6):1078-1087.
[5]DONG J,XU J,WANG X,et al.Influence of the interaction between long noncoding RNAs and hypoxia on tumorigenesis[J].Tumor Biol,2016,37(2):1379-1385.
[6]ARUN G,DIERMEIER S,AKERMAN M,et al.Differentiation of mammary tumors and reduction in metastasis upon Malat1 lncRNAloss[J].Genes Dev,2016,30(1):34-51.
[7]HU G W,WU L,KUANG W,et al.Knockdown of linc-OIP5 inhibits proliferation and migration of glioma cells through down-regulation of YAP-NOTCH signaling pathway[J].Gene,2017,610:24-31.
[8]MENDELL J T.Targeting a long noncoding RNA in breast cancer[J].N Engl J Med,2016,374(23):2287-2289.
[9]MESEURE D,ALSIBAI K D,NICOLAS A,et al.Long noncoding RNAs as new architects in cancer epigenetics,prognostic biomarkers,and potential therapeutic targets[J].Biomed Res Int,2015,2015:320214.
[10]PANDEY G K,KANDURI C.Long noncoding RNAs and neuroblastoma[J].Oncotarget,2015,6(23):18265-18275.
[11]DENG J,DENG H,LIU C,et al.Long non-coding RNA OIP5-AS1functions as an oncogene in lung adenocarcinoma through targeting mi R-448/Bcl-2[J].Biomed Pharmacother,2018,98:102-110.
[12]YANG N,CHEN J,ZHANG H,et al.LncRNA OIP5-AS1 loss-induced micro RNA-410 accumulation regulates cell proliferation and apoptosis by targeting KLF10 via activating PTEN/PI3K/AKTpathway in multiple myeloma[J].Cell Death Dis,2017,8(8):e2975.
[13]DONG J,XU J,WANG X,et al.Influence of the interaction between long noncoding RNAs and hypoxia on tumorigenesis[J].Tumor Biol,2016,37(2):1379-1385.