卵黄抗体体内代谢及中和毒素研究进展
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摘要
食品和饲料若加工、贮藏不当易产生大分子蛋白毒素和小分子毒素。许多研究者在食品和饲料中开始采用添加营养成分的方式来降低毒素毒性和预防其诱发疾病。卵黄抗体具有与抗原特异性结合的生物学特性,作为营养添加剂在中和毒素、治疗病毒和细菌性疾病等方面已得到广泛的应用。本文主要对卵黄抗体活性影响因素、体内代谢和中和蛋白大分子毒素等方面的研究进展以及发挥保护作用机制方面进行综述,最后分析讨论了卵黄抗体对小分子毒素的降毒效应研究现状和应用前景。抗毒素卵黄抗体有望被开发成口服解毒制剂,为降低食品和饲料中毒素毒性增添新的途径。
Improper food processing or storage is associated with the production of macromolecular protein toxins and small molecular toxins. Nowadays, there is an increasing interest in nutritional supplements that may counteract the detrimental effects of toxic compounds and prevent multiple diseases in humans. Egg yolk immunoglobulin(IgY) as a nutraceutical supplement for therapeutic and prophylactic intervention and against macromolecular protein toxins has been extensively studied. This article reviews recent progress in the factors affecting IgY activity, its metabolism and application for the neutralization of macromolecular protein toxins as well as the underlying mechanism. Meanwhile, we discuss the current status of understanding the detoxifying effect of IgY on small molecular toxins and future prospects for its application in this area. IgY has potential application in food and feed as an oral detoxicant.
Improper food processing or storage is associated with the production of macromolecular protein toxins and small molecular toxins. Nowadays, there is an increasing interest in nutritional supplements that may counteract the detrimental effects of toxic compounds and prevent multiple diseases in humans. Egg yolk immunoglobulin(IgY) as a nutraceutical supplement for therapeutic and prophylactic intervention and against macromolecular protein toxins has been extensively studied. This article reviews recent progress in the factors affecting IgY activity, its metabolism and application for the neutralization of macromolecular protein toxins as well as the underlying mechanism. Meanwhile, we discuss the current status of understanding the detoxifying effect of IgY on small molecular toxins and future prospects for its application in this area. IgY has potential application in food and feed as an oral detoxicant.
引文
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[26]CARLANDER D.Avian IgY antibody.in vitro and in vivo[D].Uppsala:Uppsala University,2002:13-20.
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[29]SUZUKI N,LEE Y C.Site-specific N-glycosylation of chicken serum IgG[J].Glycobiology,2004,14(3):275-292.DOI:10.1093/glycob/cwh031.
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[35]THALLEY B S,CARROLL S B.Rattlesnake and scorpion antivenoms from the egg yolks of immunized hens[J].Nature Biotechnology,1990,8(10):934-938.
[36]LIU S H,DONG W H,KONG T H.Preparation and characterization of immunoglobulin yolk against the venom of Naja naja atra[J].Indian Journal of Experimental Biology,2010,48(8):778-785.
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[2]GADDE U,RATHINAM T,LILLEHOJ H S,et al.Passive immunization with hyperimmune egg-yolk IgY as prophylaxis and therapy for poultry diseases:a review[J].Animal Health Research Reviews,2015,16(2):163-176.DOI:10.1017/S1466252315000195.
[3]XU Y P,LI X Y,JIN L J,et al.Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases:a review[J].Biotechnology Advances,2011,29(6):860-868.DOI:10.1016/j.biotechadv.2011.07.003.
[4]DIRAVIYAM T,ZHAO B,WANG Y,et al.Effect of chicken egg yolk antibodies(IgY)against diarrhea in domesticated animals:a systematic review and meta-analysis[J].PLoS ONE,2014,9(5):1-14.DOI:10.1371/journal.pone.0097716.
[5]MüLLER S,SCHUBERY A,ZAJAC J,et al.IgY antibodies in human nutrition for disease prevention[J].Nutrition Journal,2014,14(1):1-7.DOI:10.1186/s12937-015-0067-3.
[6]WANG Qin,HOU Xiaojun,CAI Kun,et al.Passive protection of purified yolk immunoglobulin administered against Shiga toxin 1 in mouse models[J].Canadian Journal of Microbiology,2010,56(12):1003-1010.DOI:10.1139/W10-087.
[7]LOEKEN M R,ROTH T F.Analysis of maternal IgG subpopulations which are transported into the chicken oocyte[J].Immunology,1983,49(1):21-28.
[8]HAMAL K R,BURGESS S C,PEVZNER I Y,et al.Maternal antibody transfer from dams to their egg yolks,egg whites,and chicks in meat lines of chickens[J].Poultry Science,2006,85(8):1364-1372.DOI:10.1093/ps/85.8.1364.
[9]SCHADE R,CALZADO E G,SARMIENTO R,et al.Chicken egg yolk antibodies(IgY-technology):a review of progress in production and use in research and human and veterinary medicine[J].Alternatives to Laboratory Animals,2005,33(2):129-154.
[10]LEE W,SYED A A,TAN S C,et al.Insights into the chicken IgY with emphasis on the generation and applications of chicken recombinant monoclonal antibodies[J].Journal of Immunological Methods,2017,447:71-85.DOI:10.1016/j.jim.2017.05.001.
[11]TIAN Z H,ZHANG X Y.Progress on research of chicken IgYantibody-FcRY receptor combination and transfer[J].Journal of Receptors and Signal Transduction Research,2012,32(5):231-237.DOI:10.3109/10799893.2012.703207.
[12]HELM B A,LING Y,TEALE C,et al.The nature and importance of the inter-epsilon chain disulfide bonds in human IgE[J].European Journal of Immunology,1991,21(6):1543-1548.DOI:10.1002/eji.1830210631.
[13]TAN S H,MOHAMEDALI A,KAPUR A,et al.A novel,cost-effective and efficient chicken egg IgY purification procedure[J].Journal of Immunological Methods,2012,380(1/2):73-76.DOI:10.1016/j.jim.2012.03.003.
[14]DE MEULENAER B,HUYGHEBAERT A.Isolation and purification of chicken egg yolk immunoglobulins:a review[J].Food and Agricultural Immunology,2001,13(4):275-288.DOI:10.1080/09540100120094537.
[15]PRIYANKA B S,ABHIJITH K S,RASTOGI K N,et al.Integrated approach for the extraction and purification of IgY from chicken egg yolk[J].Separation Science and Technology,2014,49(4):275-288.
[16]李春晖,郭立君,于洪涛,等.胃蛋白酶对抗狂犬病毒IgY活性的影响[J].中国生物制品学杂志,2002(1):45-46;54.DOI:10.3969/j.issn.1004-5503.2002.01.013.
[17]SHIMIZU M,NAGASHIMA H,SANO K,et al.Molecular stability of chicken and rabbit immunoglobulin G[J].Bioscience Biotechnology and Biochemistry,1992,56(2):270-274.DOI:10.1271/bbb.56.270.
[18]NILSSON E,ST?LBERG J,LARSSON A.IgY stability in eggs stored at room temperature or at+4℃[J].British Poultry Science,2012,53:42-46.DOI:10.1080/00071668.2011.646951.
[19]LARSSON A,KARLSSON-PARRA A,SJOQUIST J.Use of Chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors[J].Clinical Chemistry,1991,31(3):411-414.
[20]FERREIRA A J,SANTOS J P,BASSI P B,et al.IgY-Technology applied to studies of Toxoplasma gondii infection[M]//Toxoplasmosis,IntechOpen,2017:142-161.DOI:10.5772/67997.
[21]RAHMAN S,VAN NGUYEN S,ICALTO F C,et al.Oral passive IgY-based immunotherapeutics:a novel solution for prevention and treatment of alimentary tract diseases[J].Human Vaccines and Immunotherapeutics,2013,9(5):1039-1048.DOI:10.4161/hv.23383.
[22]LOSONSKY G A,JOHNSON J P,WINKELSTEIN J A,et al.Oral administration of human serum immunoglobulin in immunodeficient patients with viral gastroenteritis.a pharmacokinetic and functional analysis[J].Journal of Clinical Investigation,1985,76(6):2362-2367.DOI:10.1172/JCI112248.
[23]REILLY R M,DOMINGOL R,SANDHU J.Oral delivery of antibodies[J].Clinical Pharmacokinetics,1997,32(4):313-323.DOI:10.2165/00003088-199732040-00004.
[24]IBRAHIM E,RAHMAN A K,ISODA R,et al.In vitro and in vivo effectiveness of egg yolk antibody against Candida albicans(antiCA IgY)[J].Vaccine,2008,26(17):2073-2080.DOI:10.1016/j.vaccine.2008.02.046.
[25]YOKOYAMA H,PERALTA R C,DIAZ R,et al.Passive protective effect of chicken egg yolk against experimental enterotoxigenic Escherichia coli infection in neonatal piglets[J].Infection and Immunity,1992,60:998-1007.
[26]CARLANDER D.Avian IgY antibody.in vitro and in vivo[D].Uppsala:Uppsala University,2002:13-20.
[27]张晓萍,黄浩龙,朱祖康,等.胆囊收缩素卵黄抗体在大鼠十二指肠的吸收[J].动物学报,2004(3):375-379.DOI:10.3969/j.issn.1674-5507.2004.03.010.
[28]ZEYNALIAN H,KAZEROUNI F,EBRAHIMI F,et al.Assessment of the safety of chicken egg yolk antibody(IgY)consumption by measuring the activity of antioxidant enzymes(superoxide dismutase?catalase?glutathione peroxidase)and malondialdehyde concentration as a lipid peroxidation marker in mice[J].Journal of Paramedical Sciences,2017,8(3):29-35.
[29]SUZUKI N,LEE Y C.Site-specific N-glycosylation of chicken serum IgG[J].Glycobiology,2004,14(3):275-292.DOI:10.1093/glycob/cwh031.
[30]LI X Y,JIN L J,LU Y N,et al.Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin(IgY):effects of chitosan concentration[J].Biotechnology and Applied Biochemistry,2009,159(3):778-787.DOI:10.1007/s12010-009-8628-6.
[31]范泉水.蛇毒主要成分研究进展[J].西南国防医药,2017,27(8):901-904.DOI:10.3969/j.issn.1004-0188.2017.08.047.
[32]李江兵.眼镜蛇神经毒镇痛小肽片段的作用机制研究[D].昆明:昆明理工大学,2017:4-5.
[33]CHIPPAUX J P,GOYFFON M.Epidemiology of scorpionism:a global appraisal[J].Acta Tropica,2008,107(2):71-79.DOI:10.1016/j.actatropica.2008.05.021.
[34]ARAúJO A S,LOBATO Z I P,CHáVEZ-OLóRTEGUI C,et al.Brazilian IgY-Bothrops antivenom:Studies on the development of a process in chicken egg yolk[J].Toxicon,2010,55(4):739-744.DOI:10.1016/j.toxicon.2009.11.004.
[35]THALLEY B S,CARROLL S B.Rattlesnake and scorpion antivenoms from the egg yolks of immunized hens[J].Nature Biotechnology,1990,8(10):934-938.
[36]LIU S H,DONG W H,KONG T H.Preparation and characterization of immunoglobulin yolk against the venom of Naja naja atra[J].Indian Journal of Experimental Biology,2010,48(8):778-785.
[37]DUAN H L,HE Q Y,ZHOU B,et al.Anti-Trimeresurus albolabris venom IgY antibodies:preparation,purification and neutralization efficacy[J].Journal of Venomous Animals and Toxins Including Tropical Diseases,2016,22(1):1-6.DOI:10.1186/s40409-016-0078-3.
[38]刘锦花,李恒,和七一,等.抗蛇毒IgY对尖吻蝮(Deinagkistrodon acutus)蛇毒主要活性的中和作用研究[J].中国药理学通报,2017,33(1):58-62.DOI:10.3969/j.issn.1001-1978.2017.01.011.
[39]LEE C H,LEE Y C,LIANG M H,et al.Antibodies against venom of the snake Deinagkistrodon acutus[J].Applied and Environmental Microbiology,2015,82(1):71-80.DOI:10.1128/AEM.02608-15.
[40]LEE C H,LEE Y C,LEU S J,et al.Production and characterization of neutralizing antibodies against Bungarus multicinctus snake venom[J].Applied and Environmental Microbiology,2016,82(23):6973-6982.DOI:10.1128/AEM.01876-16.
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