低胆固醇林蛙卵油和植物甾醇(豆甾醇)在大鼠中的降脂作用（英文）
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摘要
目的:探索林蛙副产物林蛙卵油的开发价值。创新点:首次验证了低胆固醇林蛙卵油的减重降脂功能。方法:Sprague-Dawley大鼠连续被灌胃8周后,分成8组:(1)对照组喂食标准饲料;(2)高脂组喂食60%高脂饲料;(3)阳性对照组喂食3.33 mg/(kg·d)辛伐他汀;(4)OORC组喂食2 g/(kg·d)的低胆固醇林蛙卵油(OORC);(5)植物甾醇组喂食0.48g/(kg·d)的豆甾醇;(6)低剂量组喂食1g/(kg·d)的低胆固醇OORC和0.24g/(kg·d)的豆甾醇;(7)中剂量组喂食2g/(kg·d)的低胆固醇OORC和0.48 g/(kg·d)的豆甾醇;(8)高剂量组喂食4 g/(kg·d)的低胆固醇OORC和0.96 g/(kg·d)的豆甾醇。经过不同方法处理后,比较各组大鼠的体重、器官指数,以及血清中总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白浓度。同时,对动物肝脏进行组织切片染色并评价损伤情况。结论:低胆固醇林蛙卵油或者其与植物甾醇(如豆甾醇)联用可以预防高脂饮食诱导的增重、肝损伤和高血脂症状。
引文
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Zhang T,Zhao C,Zhao C,et al.,2019.Optimization of conditions for cholesterol removal from Rana chensinensis ovum oil.Curr Topics Nutraceut Res,17(2):135-139.
Bao Y,Jiang W,Liu D,et al.,2016.Advances in pharmacological research of Chinese forest frog.Mod Chin Med,18(8):1077-1080(in Chinese).https://doi.org/10.13313/j.issn.1673-4890.2016.8.031
Chen JT,Wesley R,Shamburek RD,et al.,2005.Meta-analysis of natural therapies for hyperlipidemia:plant sterols and stanols versus policosanol.Pharmacotherapy,25(2):171-183.https://doi.org/10.1592/phco.25.2.171.56942
Cholesterol Treatment Trialists’(CTT)Collaboration,2015.Efficacy and safety of LDL-lowering therapy among men and women:meta-analysis of individual data from174 000 participants in 27 randomised trials.Lancet,385(9976):1397-1405.https://doi.org/10.1016/S0140-6736(14)61368-4
Ji XF,Lin F,Qiu FP,2013.Optimization on aqueous enzymatic extraction conditions of forest frog egg protein by response surface method.J Changchun Univ Technol(Nat Sci Ed),34(2):137-144(in Chinese).https://doi.org/10.3969/j.issn.1674-1374.2013.02.005
Jin LL,Wang QY,Lou H,et al.,2004.The analysis and research of components of fatty acid in the ovum oil of Rana chensinensis.Food Sci,25(10):232-234(in Chinese).https://doi.org/10.3321/j.issn:1002-6630.2004.10.055
Nelson RH,2013.Hyperlipidemia as a risk factor for cardiovascular disease.Prim Care,40(1):195-211.https://doi.org/10.1016/j.pop.2012.11.003
Park Y,Carr TP,2013.Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines.Nutrit Res,33(2):154-161.https://doi.org/10.1016/j.nutres.2012.11.014
Wang ZY,Zhao YY,Su TT,et al.,2015.Characterization and antioxidant activity in vitro and in vivo of polysaccharide purified from Rana chensinensis skin.Carbohydr Polym,126:17-22.https://doi.org/10.1016/j.carbpol.2015.03.031
Yu L,Morishima C,Ioannou GN,2013.Dietary cholesterol intake is associated with progression of liver disease in patients with chronic hepatitis C:analysis of the hepatitis C antiviral long-term treatment against cirrhosis trial.Clin Gastroenterol Hepatol,11(12):1661-1666.https://doi.org/10.1016/j.cgh.2013.05.018
Zhang T,Zhao C,Zhao C,et al.,2019.Optimization of conditions for cholesterol removal from Rana chensinensis ovum oil.Curr Topics Nutraceut Res,17(2):135-139.