郑196耐大豆胞囊线虫病研究及耐病机理初探
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摘要
郑196是从黄淮海大豆产区选育的优良大豆品种。该品种在SCN2病圃中鼓粒情况良好;2017年,郑196在黄淮海9个试点的产量与在SCN2病圃中产量相比较,差异不显著,说明该品种具有耐SCN病的特性;利用荧光定量PCR,进一步分析来自Rhg1/Rhg4位点的4个SCN-抗性基因(Glyma18g02580, Glyma18g02590, Glyma18g02610, SHMT)在郑196及其他不同抗性水平大豆中的表达,结果表明:在受到SCN2侵染的0～25 d,4个SCN-抗性基因在抗病材料中的表达量均持续提升;在受到SCN2侵染的10 d/15 d,这4个SCN-抗性基因在郑196及感病材料中表达量达到最高点,之后表达量下降,该结果表明,郑196的耐SCN病机理不同于SCN抗性基因在抗病材料中的抗病机理,其耐病性不是由SCN抗性基因单独调控的,有可能存在其特有的耐病通路或是由抗性基因与耐病基因共同调控其耐病机制。本研究可对抗SCN种质资源创新和抵御SCN策略提供参考依据。
Zheng196 is an elite variety selected from Huang-Huai Valleys. The seed filling condition of this variety was good in SCN2 disease nursery. The yield difference between SCN-disease nursey and 9 other tested areas in 2017 was not significant which showed that it was a SCN-tolerant variety. Further analysis of the expression of four SCN-resistance genes(Glyma18 g02580, Glyma18 g02590, Glyma18 g02610) and Rhg4(SHMT) from Rhg1/Rhg4 sites in Zheng196 and other soybean with different resistance levels by fluorescence quantitative PCR. The results showed that the expression of the 4 SCN resistance genes continued to increase at 0～25 d in resistant materials while reached the highest point at 10/15 d in Zheng 196 and susceptible varieties after infected by SCN2. The results showed that the mechanism of SCN resistance of Zheng196 was different from that of SCN resistance gene in resistant materials, and the tolerance of Zheng196 was not regulated by SCN resistance gene alone. It is possible that there is a unique disease resistance pathway or that resistance genes and disease resistance genes control the mechanism of disease resistance. This study would provide useful information for the innovation of SCN germplasm resources and the defense for SCN strategy.
Zheng196 is an elite variety selected from Huang-Huai Valleys. The seed filling condition of this variety was good in SCN2 disease nursery. The yield difference between SCN-disease nursey and 9 other tested areas in 2017 was not significant which showed that it was a SCN-tolerant variety. Further analysis of the expression of four SCN-resistance genes(Glyma18 g02580, Glyma18 g02590, Glyma18 g02610) and Rhg4(SHMT) from Rhg1/Rhg4 sites in Zheng196 and other soybean with different resistance levels by fluorescence quantitative PCR. The results showed that the expression of the 4 SCN resistance genes continued to increase at 0～25 d in resistant materials while reached the highest point at 10/15 d in Zheng 196 and susceptible varieties after infected by SCN2. The results showed that the mechanism of SCN resistance of Zheng196 was different from that of SCN resistance gene in resistant materials, and the tolerance of Zheng196 was not regulated by SCN resistance gene alone. It is possible that there is a unique disease resistance pathway or that resistance genes and disease resistance genes control the mechanism of disease resistance. This study would provide useful information for the innovation of SCN germplasm resources and the defense for SCN strategy.
引文
Ali M.A.,Abbas A.,Aze em F.,Javed N.,and Bohlmann H.2015,Plant-nematode interactions:from genomics to metabolomics,Int.J.Agric.Biol.,17(6):1071-1082
Ali M.A.,Azeem F.,Li H.,and Bohlmann H.,2017,Smart parasitic nematodes use multifaceted strategies to parasitize plants Front.Plant Sci.,8:1699
Allen T.W.,2017,Soybean yield loss estimates due to diseases in the united states and ontario,canada,from 2010 to 2014Plant Health Progress,18:19-27
Bellafiore S.,Shen Z.X.,Rosso M.N.,Abad P.,Shih P.,and Briggs S.P.,2008,Direct identi覱cation of the Meloidogyne incognita secretome reveals proteins with host cell reprogramming potential,PLoS Pathog,4(10):e1000192
Budischak S.A.,Hansen C.B.,Caudron Q.,Garnier R.,Kartzine T.R.,Pelczer I.,Cressler C.E.,van Leeuwen A.,and Graham A.L.,2018,Feeding immunity:Physiological and behaviora responses to infection and resource limitation,Front.Immunol.,8:1914
Caillaud M.C.,Dubreuil G.,Quentin M.,Perfus-Barbeoch L.Lecomte P.,de Almeida Engler J.,Abad P.,Rosso M.N.,and Favery B.,2008,Root-knot nematodes manipulate plant cel functions during a compatible interaction,J.Plant Physiol.165(1):104-113
Cook D.E.,Lee T.G.,Guo X.,Melito S.,Wang K.,Bayless A.M.,Wang J.,Hughes T.J.,Willis D.K.,Clemente T.E.,Diers B.W.,Jiang J.,Hudson M.E.,and Bent A.F.,2012,Copy number variation of multiple genes at Rhg1 mediates nematode resistance in soybean,Science,338(6111):1206-1209
Davis E.L.,and Mitchum M.G.,2005,Nematodes.Sophisticated parasites of legumes,Plant Physiol.,137(4):1182-1188
Gheysen G.,and Mitchum M.G.,2011,How nematodes manipulate plant development pathways for infection,Curr.Opin.Plant Biol.,14(4):415-421
Goverse A.,and Smant G.,2014,The activation and suppression of plant innate immunity by parasitic nematodes,Annu.Rev.Phytopathol.,52(1):243-265
Haegeman A.,Mantelin S.,Jones J.T.,and Gheysen G.,2012,Functional roles of effectors of plant-parasitic nematodes,Gene,492(1):19-31
Jaouannet M.,Magliano M.,Arguel M.J.,Gourgues M.,Evangelisti E.,Abad P.,and Rosso M.N.,2013,The root-knot nematode calreticulin Mi-CRT is a key effector in plant defense suppression,Mol.Plant-Microbe Interact.,26(1):97-105
Kennedy M.J.,Niblack T.L.,and Krishnan H.B.,1999,Infection by Heterodera glycines elevates isoflavonoid production and influences soybean nodulation,J.Nematol.,31(3):341-347
Koenning S.R.,and Wrather J.A.,2010,Suppression of soybean yield potential in the continental United States from plant diseases estimated from 2006 to 2009,Plant Health Progress,10:1094
Lee S.G.,and Jez J.M.,2014,Nematode phospholipid metabolism:an example of closing the genome-structure-function circle,Trends Parasitol.,30(5):241-250
Lian Y.,Guo J.Q.,Li H.C.,Wu Y.K.,Wei H.,Wang J.S.,Li J.Y.,and Lu W.G.,2017,A new race(X12)of soybean cyst nematode in China,J.Nematol.,49(3):321-326
Lian Y.,Wang J.S.,Li H.C.,Wei H.,Li J.Y.,Wu Y.K.,Lei C.F.,Zhang H.,Wang S.F.,Guo J.Q.,Li Y.X.,Li Z.H.,Jin Q.L.,Xu S.X.,Zhang Z.M.,Yang C.Y.,Yu H.Y.,Geng Z.,Shu W.T.,and Lu W.G.,2016,Race distribution of soybean cyst nematode in the main soybean producing area of Huang-Huai Rivers Valley,Zuowu Xuebao(Acta Agronomica Sinica),42(10):1479-1486(练云,王金社,李海朝,魏荷,李金英,武永康,雷晨芳,张辉,王树峰,郭建秋,李月霞,李志辉,靳巧玲,徐淑霞,张志民,杨彩云,于会勇,耿臻,舒文涛,卢为国,2016,黄淮大豆主产区大豆胞囊线虫生理小种分布调查,作物学报,42(10):1479-1486)
Liu S.,Kandoth P.K.,Warren S.D.,Yeckel G.,Heinz R.,Alden J.,Yang C.,Jamai A.,EI-Mellouki T.,Juvale P.S.,Hill J.,Baum T.J.,Cianzio S.,Whitham S.A.,Korkin D.,Mitchum M.G.,and Meksem K.,2012,A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens,Nature,492(7428):256-260
Lu W.G.,Gai J.Y.,and Li W.D.,2006,Sampling survey and identification of races of soybean cyst nematode(Heterodera glycines ichinohe)in Huang-Huai valleys,Zhongguo Nongye Kexue(Scientia Agriuclura Sinica),39(2):306-312(卢为国盖钧镒,李卫东,2006,黄淮地区大豆胞囊线虫生理小种的抽样调查与研究,中国农业科学,39(2):306-312)
Mitchum M.G.,Hussey R.S.,Baum T.J.,Wang X.H.,Elling A.A.Wubben M.,and Davis E.L.,2013,Nematode effector proteins:an emerging paradigm of parasitism,New Phytol.,199(4):879-894
Mundt C.C.,2015,Durable resistance:a key to sustainable management of pathogens and pests,Infect.Genet.Evol.,27446-455
Mundt C.C.,Cowger C.C.,and Garrett K.A.,2002,Relevance of integreated disease management to resistance durability,Euphytica,124(2):245-252
Nelson R.,Wiesner-Hanks T.,Wisser R.,and Balint-kurti P.,2018,Navigating complexity to breed disease-resistant crops,Nat.Rev.Genet.,19(1):21-33
Niblack T.L.,Wrather J.A.,Heinz R.D.,and Donald P.A.,2003,Distribution and virulence phenotypes of Heterodera glycines in Missouri,Plant Disease,87(8):929-932
Thapa S.,Patel J.A.,Reuter-Carlson U.,and Schroeder N.E.,2017,Embryogenesis in the parasitic nematode Heterodera glycines is independent of host-derived hatching stimulation,BMC Dev.Biol.,17(1):2
Ali M.A.,Azeem F.,Li H.,and Bohlmann H.,2017,Smart parasitic nematodes use multifaceted strategies to parasitize plants Front.Plant Sci.,8:1699
Allen T.W.,2017,Soybean yield loss estimates due to diseases in the united states and ontario,canada,from 2010 to 2014Plant Health Progress,18:19-27
Bellafiore S.,Shen Z.X.,Rosso M.N.,Abad P.,Shih P.,and Briggs S.P.,2008,Direct identi覱cation of the Meloidogyne incognita secretome reveals proteins with host cell reprogramming potential,PLoS Pathog,4(10):e1000192
Budischak S.A.,Hansen C.B.,Caudron Q.,Garnier R.,Kartzine T.R.,Pelczer I.,Cressler C.E.,van Leeuwen A.,and Graham A.L.,2018,Feeding immunity:Physiological and behaviora responses to infection and resource limitation,Front.Immunol.,8:1914
Caillaud M.C.,Dubreuil G.,Quentin M.,Perfus-Barbeoch L.Lecomte P.,de Almeida Engler J.,Abad P.,Rosso M.N.,and Favery B.,2008,Root-knot nematodes manipulate plant cel functions during a compatible interaction,J.Plant Physiol.165(1):104-113
Cook D.E.,Lee T.G.,Guo X.,Melito S.,Wang K.,Bayless A.M.,Wang J.,Hughes T.J.,Willis D.K.,Clemente T.E.,Diers B.W.,Jiang J.,Hudson M.E.,and Bent A.F.,2012,Copy number variation of multiple genes at Rhg1 mediates nematode resistance in soybean,Science,338(6111):1206-1209
Davis E.L.,and Mitchum M.G.,2005,Nematodes.Sophisticated parasites of legumes,Plant Physiol.,137(4):1182-1188
Gheysen G.,and Mitchum M.G.,2011,How nematodes manipulate plant development pathways for infection,Curr.Opin.Plant Biol.,14(4):415-421
Goverse A.,and Smant G.,2014,The activation and suppression of plant innate immunity by parasitic nematodes,Annu.Rev.Phytopathol.,52(1):243-265
Haegeman A.,Mantelin S.,Jones J.T.,and Gheysen G.,2012,Functional roles of effectors of plant-parasitic nematodes,Gene,492(1):19-31
Jaouannet M.,Magliano M.,Arguel M.J.,Gourgues M.,Evangelisti E.,Abad P.,and Rosso M.N.,2013,The root-knot nematode calreticulin Mi-CRT is a key effector in plant defense suppression,Mol.Plant-Microbe Interact.,26(1):97-105
Kennedy M.J.,Niblack T.L.,and Krishnan H.B.,1999,Infection by Heterodera glycines elevates isoflavonoid production and influences soybean nodulation,J.Nematol.,31(3):341-347
Koenning S.R.,and Wrather J.A.,2010,Suppression of soybean yield potential in the continental United States from plant diseases estimated from 2006 to 2009,Plant Health Progress,10:1094
Lee S.G.,and Jez J.M.,2014,Nematode phospholipid metabolism:an example of closing the genome-structure-function circle,Trends Parasitol.,30(5):241-250
Lian Y.,Guo J.Q.,Li H.C.,Wu Y.K.,Wei H.,Wang J.S.,Li J.Y.,and Lu W.G.,2017,A new race(X12)of soybean cyst nematode in China,J.Nematol.,49(3):321-326
Lian Y.,Wang J.S.,Li H.C.,Wei H.,Li J.Y.,Wu Y.K.,Lei C.F.,Zhang H.,Wang S.F.,Guo J.Q.,Li Y.X.,Li Z.H.,Jin Q.L.,Xu S.X.,Zhang Z.M.,Yang C.Y.,Yu H.Y.,Geng Z.,Shu W.T.,and Lu W.G.,2016,Race distribution of soybean cyst nematode in the main soybean producing area of Huang-Huai Rivers Valley,Zuowu Xuebao(Acta Agronomica Sinica),42(10):1479-1486(练云,王金社,李海朝,魏荷,李金英,武永康,雷晨芳,张辉,王树峰,郭建秋,李月霞,李志辉,靳巧玲,徐淑霞,张志民,杨彩云,于会勇,耿臻,舒文涛,卢为国,2016,黄淮大豆主产区大豆胞囊线虫生理小种分布调查,作物学报,42(10):1479-1486)
Liu S.,Kandoth P.K.,Warren S.D.,Yeckel G.,Heinz R.,Alden J.,Yang C.,Jamai A.,EI-Mellouki T.,Juvale P.S.,Hill J.,Baum T.J.,Cianzio S.,Whitham S.A.,Korkin D.,Mitchum M.G.,and Meksem K.,2012,A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens,Nature,492(7428):256-260
Lu W.G.,Gai J.Y.,and Li W.D.,2006,Sampling survey and identification of races of soybean cyst nematode(Heterodera glycines ichinohe)in Huang-Huai valleys,Zhongguo Nongye Kexue(Scientia Agriuclura Sinica),39(2):306-312(卢为国盖钧镒,李卫东,2006,黄淮地区大豆胞囊线虫生理小种的抽样调查与研究,中国农业科学,39(2):306-312)
Mitchum M.G.,Hussey R.S.,Baum T.J.,Wang X.H.,Elling A.A.Wubben M.,and Davis E.L.,2013,Nematode effector proteins:an emerging paradigm of parasitism,New Phytol.,199(4):879-894
Mundt C.C.,2015,Durable resistance:a key to sustainable management of pathogens and pests,Infect.Genet.Evol.,27446-455
Mundt C.C.,Cowger C.C.,and Garrett K.A.,2002,Relevance of integreated disease management to resistance durability,Euphytica,124(2):245-252
Nelson R.,Wiesner-Hanks T.,Wisser R.,and Balint-kurti P.,2018,Navigating complexity to breed disease-resistant crops,Nat.Rev.Genet.,19(1):21-33
Niblack T.L.,Wrather J.A.,Heinz R.D.,and Donald P.A.,2003,Distribution and virulence phenotypes of Heterodera glycines in Missouri,Plant Disease,87(8):929-932
Thapa S.,Patel J.A.,Reuter-Carlson U.,and Schroeder N.E.,2017,Embryogenesis in the parasitic nematode Heterodera glycines is independent of host-derived hatching stimulation,BMC Dev.Biol.,17(1):2
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