高温液态水预处理园林废弃物提高酶解率
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摘要
为探寻园林废弃物的能源化再利用潜力,以红花羊蹄甲树枝和台湾草废弃物为原料,采用高温液态水预处理方法进行工艺优化研究,以提高其酶解率。结果表明:红花羊蹄甲树枝的最优P因子为1233,台湾草废弃物的最优P因子为153,此时总木糖收率分别为85.48%和68.40%,总糖收率分别达到87.84%和86.31%,72 h酶解率分别由原料的43.99%和55.94%提高到81.25%和87.27%,其中台湾草废弃物比红花羊蹄甲树枝在预处理前后都更易酶解。高温液态水预处理过程脱除了大量半纤维和部分木质素,显著改变了物料表面平整结构,增大了比表面积和纤维素结晶指数,这些变化有助于后续酶解。
In order to explore the energy reuse potential of garden waste,Bauhinia blakeana Dunn branches and Zoysia tenuifolia waste were selected as raw materials,and they were pretreated with liquid hot water to obtain the process optimization results and improve the enzymatic digestibility. The results indicated that the optimal P-factor of Bauhiniablakeana Dunn branches and Zoysia tenuifolia waste was 1233 and 153 respectively. At this time,the Bauhinia blakeanaDunn branches and Zoysia tenuifolia waste got total xylose yield of 85.48% and 68.40%,total sugar recovery of 87.84% and 86.31%,and 72 h enzymatic digestibility of 81.25% raised from 43.99% and 87.27% raised from 55.94% respectively,in addition,Zoysia tenuifolia waste had better properties of enzymatic hydrolysis than Bauhinia blakeana Dunn branches. Liquid hot water pretreatment removed a large number of hemicelluloses and some lignin,there was a significant change in surface structure after pretreatment,and it also increased the specific surface area and cellulose crystallinity index,all of the changes could help to improve the accessibility of cellulose to enzyme and increase the enzymatic digestibility.
In order to explore the energy reuse potential of garden waste,Bauhinia blakeana Dunn branches and Zoysia tenuifolia waste were selected as raw materials,and they were pretreated with liquid hot water to obtain the process optimization results and improve the enzymatic digestibility. The results indicated that the optimal P-factor of Bauhiniablakeana Dunn branches and Zoysia tenuifolia waste was 1233 and 153 respectively. At this time,the Bauhinia blakeanaDunn branches and Zoysia tenuifolia waste got total xylose yield of 85.48% and 68.40%,total sugar recovery of 87.84% and 86.31%,and 72 h enzymatic digestibility of 81.25% raised from 43.99% and 87.27% raised from 55.94% respectively,in addition,Zoysia tenuifolia waste had better properties of enzymatic hydrolysis than Bauhinia blakeana Dunn branches. Liquid hot water pretreatment removed a large number of hemicelluloses and some lignin,there was a significant change in surface structure after pretreatment,and it also increased the specific surface area and cellulose crystallinity index,all of the changes could help to improve the accessibility of cellulose to enzyme and increase the enzymatic digestibility.
引文
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[2] Shi Yan,Ge Ying,Chang Jie,et al. Garden wastebiomass for renewable and sustainable energyproduction in China:Potential, challenges anddevelopment[J]. Renewable&Sustainable EnergyReviews,2013,22(8):432—437.
[3] Wani K A,Mamta,Rao R J. Bioconversion of gardenwaste,kitchen waste and cow dung into value-addedproducts using earthworm Eisenia fetida[J]. SaudiJournalofBiologicalSciences,2013,20(2):149—154.
[4] Kumar A, Singh L K, Ghosh S. Bioconversion oflignocellulosic fraction of water-hyacinth(Eichhorniacrassipes)hemicellulose acid hydrolysate to ethanol byPichia stipitis[J]. Bioresource Technology,2009,100(13):3293—3297.
[5]余强,庄新姝,袁振宏,等.木质纤维素类生物质制取燃料及化学品的研究进展[J].化工进展,2012,31(4):784—791.[5] Yu Qiang,Zhuang Xinshu,Yuan Zhenhong,et al.Research progress on fuel and chemicals productionfrom lignocellulose biomass[J]. Chemical Industry andEngineering Progress,2012,31(4):784—791.
[6] Alvira P, Tomás-PejóE, Ballesteros M, et al.Pretreatment technologies for an efficient bioethanolproduction process based on enzymatic hydrolysis:Areview[J]. Bioresource Technology,2010,101(13):4851—4861.
[7]余强,庄新姝,袁振宏,等.高温液态水两步预处理提高桉木酶解率[J].太阳能学报,2011,32(8):1129—1133.[7] Yu Qiang,Zhuang Xinshu,Yuan Zhenhong,et al.Liquid hot water pretreatment of eucalyptus grandis toenhance enzymatic digestibility of cellulose[J]. ActaEnergiae Solaris Sinica,2011,32(8):1129—1133.
[8] Zhuang Xinshu,Wang Wen,Yu Qiang,et al. Liquidhot water pretreatment of lignocellulosic biomass forbioethanol production accompanying with high valuableproducts[J]. Bioresource Technology,2016,199:68—75.
[9] Mosier N,Hendrickson R,Ho N,et al. Optimization ofpH controlled liquid hot water pretreatment of corn stover[J]. Bioresource Technology,2005,96(18):1986—1993.
[10] Sluiter A,Hames B,Ruiz R,et al. Determination ofstructural carbohydrates and lignin in biomass[R].Technical Report NREL/TP-510-42623,2008.
[11] Zhuang Xinshu,Yuan Zhenhong,Ma Longlong,et al.Kinetic study of hydrolysis of xylan and agriculturalwastes with hot liquid water[J]. BiotechnologyAdvances,2009,27(5):578—582.
[12] Sixta H. Handbook of pulp[M]. Weinheim,Germany:Wiley-vch,2006.
[13]陈嘉翔.制浆化学[M].北京:中国轻工业出版社,1990:230—235.[13] Chen Jiaxiang. Pulping chemistry[M]. Beijing:ChinaLight Industry Press,1990:230—235.
[14] Yu Qiang,Zhuang Xinshu,Lyu Shuangliang,et al.Liquid hot water pretreatment of sugarcane bagasse andits comparison with chemical pretreatment methods forthe sugar recovery and structural changes[J].Bioresource Technology,2013,129(2):592—598.