高、低糖甘蔗品种苗期糖分含量及代谢相关酶活性分析
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摘要
以高糖(GT35)和低糖(B8)甘蔗品种苗期不同部位的叶和茎为材料,采用HPLC技术测定蔗糖、葡萄糖和果糖含量,分析2个甘蔗品种叶和茎中蔗糖代谢相关酶活性与糖分含量的相关性和差异性。结果表明:2个甘蔗品种苗期叶中蔗糖含量与SPS和SS-s酶活性呈显著正相关,茎中己糖含量与NI酶活性呈显著正相关,茎中蔗糖含量与SPS酶活性呈显著正相关,而与SS-c、SAI和CIN酶活性呈显著负相关。GT35茎中蔗糖含量显著高于B8,叶中蔗糖含量显著低于B8。GT35茎中SPS和叶中SS-s酶活性均显著高于B8,茎中SS-s和SS-c酶活性低于B8,其中只有幼茎中SS-c酶活性与B8差异不显著。说明苗期叶中高SS-s酶活性,茎中高SPS酶活性,低SS-s和SS-c酶活性,可能是调节高糖甘蔗品种苗期茎中蔗糖积累的重要因素。
The stems and leaves of different maturity at the seedling stage of high sugar(GT35) and low sugar(B8)sugarcane were used as the materials,and the contents of sucrose,glucose and fructose were measured using high performance liquid chromatography(HPLC),to analyze the correlation and difference between sucrose metabolic enzymes and sugar accumulation in high and low sugar sugarcane.The sucrose content in the leaves was positively correlated with the activity of sucrose phosphate synthase(SPS) and sucrose synthase in the synthesis direction(SS-s).The hexose content in the stem was positively correlated with the activity of neutral/alkaline invertase(NI),the sucrose content in the stem was positively correlated with the activity of SPS,and the activity of sucrose synthetase in the cleavage direction(SS-c),soluble acid invertase(SAI) and cell wall-bound invertase(CIN) was negatively correlated.Sucrose content in the stem of GT35 was significantly higher than that of B8,and sucrose content in the leaf was significantly lower than that of B8.The SPS activity in stems and SS-s activity leaves of GT35 were significantly higher than that of B8,while the SS-s and SS-c activity in GT35 stems was lower than that of B8,but only the SS-c activity in young stems of GT35 was not significantly lower than of B8.It indicates that high SS-s activity in leaves,high SPS activity in stems,low SS-s and SS-c activity in stems may be important factors for regulating sucrose accumulation in high-sugar varieties of sugarcane at seedling stage.
The stems and leaves of different maturity at the seedling stage of high sugar(GT35) and low sugar(B8)sugarcane were used as the materials,and the contents of sucrose,glucose and fructose were measured using high performance liquid chromatography(HPLC),to analyze the correlation and difference between sucrose metabolic enzymes and sugar accumulation in high and low sugar sugarcane.The sucrose content in the leaves was positively correlated with the activity of sucrose phosphate synthase(SPS) and sucrose synthase in the synthesis direction(SS-s).The hexose content in the stem was positively correlated with the activity of neutral/alkaline invertase(NI),the sucrose content in the stem was positively correlated with the activity of SPS,and the activity of sucrose synthetase in the cleavage direction(SS-c),soluble acid invertase(SAI) and cell wall-bound invertase(CIN) was negatively correlated.Sucrose content in the stem of GT35 was significantly higher than that of B8,and sucrose content in the leaf was significantly lower than that of B8.The SPS activity in stems and SS-s activity leaves of GT35 were significantly higher than that of B8,while the SS-s and SS-c activity in GT35 stems was lower than that of B8,but only the SS-c activity in young stems of GT35 was not significantly lower than of B8.It indicates that high SS-s activity in leaves,high SPS activity in stems,low SS-s and SS-c activity in stems may be important factors for regulating sucrose accumulation in high-sugar varieties of sugarcane at seedling stage.
引文
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[21]章英才,陈亚萍,景红霞,等.‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系[J].果树学报,2014,31(2):250-257.
[22]Liu Y J,Wang G L,Ma J,et al.Transcript profiling of sucrose synthase genes involved in sucrose metabolism among four carrot(Daucus carota L.)cultivars reveals distinct patterns[J].Bmc Plant Biology,2018,18(1):1-12.
[23]Sachdeva M,Mann A P S,Batta S K.Sucrose metabolism and expression enzyme activities in low and high storing sugarcane genotypes of key sucrose[J].Sugar Tech,2003,5(4):265-271.
[24]罗霄,郑国琦,王俊.果实糖代谢及其影响因素的研究进展[J].农业科学研究,2008,29(2):69-74.
[2]赵丽宏,王俊刚,杨本鹏,等.甘蔗体内的蔗糖积累[J].基因组学与应用生物学,2009,28(2):385-390.
[3]秦翠鲜,桂意云,陈忠良,等.植物蔗糖合成酶基因研究进展[J].分子植物育种,2018,16(12):3907-3914.
[4]Sturm A.Invertases:primary structures,functions and roles in plant development and sucrose partitioning[J].Plant Physiology,1999,121(1):l-8.
[5]Wan H,Wu L,Yang Y,et al.Evolution of sucrose metabolism:the dichotomy of invertases and beyond[J].Trends in Plant Science,2018,23(2):163-177.
[6]周文灵,江永,李奇伟,等.甘蔗蔗糖积累的规律、影响因素及其调控机制的研究进展[J].甘蔗糖业,2011,40(6):11-17.
[7]Zhu Y J,Albert H H,Moore P H.Differential expression of soluble acid invertase genes in the shoots of high-sucrose and low-sucrose species of Saccharum and their hybrids[J].Australian Journl of Plant Physiology,2000,27(3):193-199.
[8]牛俊奇,Phan Thi Thu,邵敏,等.甘蔗工艺成熟期转化酶及其抑制子与蔗糖积累的相关性研究[J].西南农业学报,2015,28(4):160-1611.
[9]Pan Y Q,Lou H L,Li Y R.Soluble acid invertase and sucrose phosphate synthase:key enzymes in regulating sucrose accumulation in sugarcane stalk[J].Sugar Tech,2009,11(1):28-33.
[10]武红霞,姚全胜,王松标,等.‘KRS’芒果发育过程中糖积累与代谢相关酶活性的关系[J].安徽农业科学,2016,44(17):24-26,55.
[11]牛俊奇,黄静丽,赵文慧,等.甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究[J].生物技术通报,2015,31(9):105-110
[12]陈兰平,陈由强,方静平,等.甘蔗SPS基因家族成员表达与糖分积累关系解析[J].热带作物学报,2014,35(7):1354-1361.
[13]张明方,李志凌.高等植物中与蔗糖代谢相关的酶[J].植物生理学通讯,2002,38(3):289-295
[14]王俊刚,赵婷婷,张树珍,等.甘蔗体内的蔗糖转运与运输途径[J].植物生理学通讯,2008,3:605-611.
[15]闫梅玲,代红军,单守明,等.蔗糖代谢相关酶对果实糖积累影响的研究进展[J].安徽农业科学,2009,37(29):14021-14023.
[16]田代发,宋焕忠.甘蔗生长发育特性与高产栽培技术[J].种子科技,2015,33(3):49-50,52.
[17]王嘉佳,唐中华.可溶性糖对植物生长发育调控作用的研究进展[J].植物学研究,2014,3:71-76.
[18]谭中文,梁计南,陈建平,等.甘蔗苗期生化性状与糖分及产量的关系[J].甘蔗,2003,10(2):1-5.
[19]艾买尔江.吾斯曼,王冀川,等.不同基因型甜高粱糖分积累规律研究[J].新疆农垦科技,2015,38(1):47-48.
[20]Sachdeva M,Bhatia S,Batta S K.Sucrose accumulation in sugarcane:a potential target for crop improvement[J].Acta Physiology Plant,2011,33(5):1571-1583.
[21]章英才,陈亚萍,景红霞,等.‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系[J].果树学报,2014,31(2):250-257.
[22]Liu Y J,Wang G L,Ma J,et al.Transcript profiling of sucrose synthase genes involved in sucrose metabolism among four carrot(Daucus carota L.)cultivars reveals distinct patterns[J].Bmc Plant Biology,2018,18(1):1-12.
[23]Sachdeva M,Mann A P S,Batta S K.Sucrose metabolism and expression enzyme activities in low and high storing sugarcane genotypes of key sucrose[J].Sugar Tech,2003,5(4):265-271.
[24]罗霄,郑国琦,王俊.果实糖代谢及其影响因素的研究进展[J].农业科学研究,2008,29(2):69-74.