牛乳源性无乳链球菌表面蛋白sip及pgk对奶牛的免疫保护试验
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- 英文论文题名：Immunity protective test of dairy cow mastitis streptococcus agalactiae surface protein SIP and PGK to dairy cow
- 论文作者：吴金花 ; 锡林高娃 ; 布日额 ; 白文丽
- 英文论文作者：WU Jin-hua ; XI Lin gao wa ; BU Ri-e ; BAI wen-li ; Inner Mongolia Autonomous Region Engineering Technology Research Center of Prevention and Control the Pathogenic Bacteria in Milk ; College of Life Science ; Inner Mongolia University for Nationalities ; Research Institute of Pathogenic in Milk ; Inner Mongolia University for Nationalities
- 年：2016
- 作者机构：内蒙古乳源性致病菌防控工程技术研究中心;内蒙古民族大学生命科学学院;内蒙古民族大学乳源性致病菌研究所;
- 论文关键词：无乳链球菌 ; 牛乳腺炎 ; 亚单位抗原 ; 免疫
- 英文论文关键词：Streptococcus agalactiae ; pgk subunit ; Immune efficience
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：中文
- 分类号：S858.23
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：5
- 文件大小：525k
- 原文格式：D
- 会议级别：全国
摘要
目的评价牛乳腺炎无乳链球菌表面亚单位抗原SIP、PGK对奶牛的免疫保护性。方法通过制备SIP、PGK、SIP+PGK三种亚单位抗原乳化免疫制剂,分别对科尔沁奶牛进行免疫试验,检测免疫0d、14d、28d、42d、56d、70d、84d的血清抗体滴度,同时设立牛乳腺炎无乳链球菌全菌体裂解免疫制剂免疫组作为对照。在三免后14d利用10×105 CFU/m L的无乳链球菌临床分离株进行乳头感染试验,同时设立非免疫牛对照组。通过感染抑制率统计出免疫保护效果。结果免疫后结果表明SIP、PGK单一抗原免疫后第14d、28d、42d、56d、70d、84d的血清抗体平均滴度分别为1:1200、1:2800、1:4600、1:6600、1:5200、1:4000和1:1000、1:2700、1:4500、1:6400、1:5300、1:3500;全菌体裂解抗原免疫组抗体平均滴度为1:800、1:1600、1:3400、1:4100、1:3400、1:2800;混合抗原免疫后其抗体滴度平均为1:1400、1:3600、1:5400、1:7800、1:9600、1:6000。免疫保护试验结果显示,SIP+PGK混合抗原的免疫保护率达到83.3%,而SIP、PGK抗原及全菌体裂解抗原单独免疫组的免疫保护率分别为33.3%、41.6%、58.3%。结论牛乳腺炎无乳链球菌表面蛋白SIP+PGK混合抗原的免疫保护率优于该两种抗原的单独免疫效果,差异显著(p<0.05),可以对奶牛进行临床免疫来预防无乳链球菌性乳腺炎。
diary mastitis streptococcus agalactiae.Methods The three kind of emulsity immunity agents were prepared and were tested for protective immunity to diary cattle.The antibody titer of sera for immunized at 0d,14 d,28 d,42 d,56 d,70 d and 84 d were determined.The group of immunized with whole cell lysitive antigen was asthe control group.Result The result showed that the sera antibody titer of SIP and PGK single antigen after immune 14 d,28 d,42 d,56 d,70 d,84 d were 1:1200,1:2800,1:4600,1:6600,1:5200,1:4000;1:1000,1:2700,1:4500、1:6400、1:5300、1:3500,respectively.The sera antibody titer of the whole cell lysis immunity antigen were1:800、1:1600、1:3400、1:4100、1:3400、1:2800.And the sera antibody titer of SIP+PGK mixed antigen were1:1400、1:3600、1:5400、1:7800、1:9600、1:6000.The immunity protective assay result showed that the protective rate was 83.3% of the SIP+PGK mixed antigens,and the protective rate of SIP,PGK antigen and whole cell lysis antigen were 33.3%,41.6% and 58.3%,respectively.Conclusion The immunity protective rate of SIP+PGK fusion antigens were better than the immunity protective rate of the two antigens were immuned with respectively.The effect was difference(p<0.05).They were could be used to prevention of dairy cow mastitis.
diary mastitis streptococcus agalactiae.Methods The three kind of emulsity immunity agents were prepared and were tested for protective immunity to diary cattle.The antibody titer of sera for immunized at 0d,14 d,28 d,42 d,56 d,70 d and 84 d were determined.The group of immunized with whole cell lysitive antigen was asthe control group.Result The result showed that the sera antibody titer of SIP and PGK single antigen after immune 14 d,28 d,42 d,56 d,70 d,84 d were 1:1200,1:2800,1:4600,1:6600,1:5200,1:4000;1:1000,1:2700,1:4500、1:6400、1:5300、1:3500,respectively.The sera antibody titer of the whole cell lysis immunity antigen were1:800、1:1600、1:3400、1:4100、1:3400、1:2800.And the sera antibody titer of SIP+PGK mixed antigen were1:1400、1:3600、1:5400、1:7800、1:9600、1:6000.The immunity protective assay result showed that the protective rate was 83.3% of the SIP+PGK mixed antigens,and the protective rate of SIP,PGK antigen and whole cell lysis antigen were 33.3%,41.6% and 58.3%,respectively.Conclusion The immunity protective rate of SIP+PGK fusion antigens were better than the immunity protective rate of the two antigens were immuned with respectively.The effect was difference(p<0.05).They were could be used to prevention of dairy cow mastitis.
引文
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[3]吴金花.通辽地区奶牛乳房炎主要病原菌分离及药物敏感性试验[J].黑龙江畜牧兽医,2007,3:77-78.
[4]Olikoronou G,Machado VS,Santisteban C,et al.Microbial diversity of bovine mastitis milk as described by pyrosequencing of metagenomic 16S r DNA[J].Olo S One,2012,7(10):e 47671.
[5]Mesele A,Tadios H,Kassaye A,et al.Major cause of mastitis and associated risk factors in smallholder dairy farms in and around Hawassa,Southern Ethiopia[J].Tropical Animal health and Production,Online first,9January 2012.
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[7]张海宝,布日额,吴金花,等.牛乳腺炎无乳链球菌表面蛋白pgk抗原优势区的原核表达及其间接ELISA检测方法的建立.中国预防兽医学报,2013,4:312-316.
[8]郎景民,布日额,吴金花,等.牛源无乳链球菌sip蛋白抗原优势区的原核表达及其间接ELISA检测方法的建立.中国预防兽医学报,2011,(33)1:37-40.
[9]Lindahl G.Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens[J].Clin Microbiol Rev,2005,18(1):102-127.
[2]布日额,刘娣,吴金花,等.内蒙古地区奶牛优势性无乳链球菌的分离与鉴定[J].黑龙江畜牧兽医,2009,5.
[3]吴金花.通辽地区奶牛乳房炎主要病原菌分离及药物敏感性试验[J].黑龙江畜牧兽医,2007,3:77-78.
[4]Olikoronou G,Machado VS,Santisteban C,et al.Microbial diversity of bovine mastitis milk as described by pyrosequencing of metagenomic 16S r DNA[J].Olo S One,2012,7(10):e 47671.
[5]Mesele A,Tadios H,Kassaye A,et al.Major cause of mastitis and associated risk factors in smallholder dairy farms in and around Hawassa,Southern Ethiopia[J].Tropical Animal health and Production,Online first,9January 2012.
[6]Sentitula B R,Yadav and Ravinder K.Incidence of staphylococci and Streptococcus agalactiea during winter in mastitis milk of Sahiwal cow and murrah Buffaloes[J].Indian J Microbilogy,Online First,8 August 2011.
[7]张海宝,布日额,吴金花,等.牛乳腺炎无乳链球菌表面蛋白pgk抗原优势区的原核表达及其间接ELISA检测方法的建立.中国预防兽医学报,2013,4:312-316.
[8]郎景民,布日额,吴金花,等.牛源无乳链球菌sip蛋白抗原优势区的原核表达及其间接ELISA检测方法的建立.中国预防兽医学报,2011,(33)1:37-40.
[9]Lindahl G.Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens[J].Clin Microbiol Rev,2005,18(1):102-127.