黑鲷增殖放流个体分子判别方法的建立与应用
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- 英文论文题名：The establishment and application of molecular identification method of releasing-recapture individuals for black sea bream, Sparus macrocephlus
- 论文作者：付亚男 ; 李纯厚 ; 林琳
- 英文论文作者：Fu Yanan ; Li Chunhou ; Lin Lin ; South China Sea Fishery Resources Development and Utilization Laboratory ; Ministry of Agriculture/Key Laboratory of Fishery Ecology and Environment ; Guangdong Province/South China Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; College of Fisheries and Life Science ; Shanghai Ocean University
- 年：2016
- 作者机构：农业部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室/中国水产科学研究院南海水产研究所;上海海洋大学,水产与生命学院;
- 论文关键词：黑鲷 ; 系谱分析 ; 微卫星 ; 增殖放流
- 英文论文关键词：Black sea bream ; Parentage analysis ; Microsatellite ; Stock enhancement
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S931.5
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：560k
- 原文格式：D
- 会议级别：全国
摘要
本研究使用系谱分析技术,建立了黑鲷放流个体分子判别方法,并在放流实践中对方法进行了应用。首先,选取102尾黑鲷亲本,隔离催卵,孵化受精卵;采用"高通量测序法"开发获得黑鲷"分子判别微卫星标记组",并对亲本群体进行等位基因分型;将分型数据输入系谱分析软件Cervus,进行模拟判别,以评估标记组的判别能力,确定判别标准;之后,将黑鲷幼鱼培育至放流规格,在大亚湾进行放流,并回捕。最后,使用上述标记组和判别标准完成对回捕群体中放流个体的判别。结果,共开发获得多态性黑棘鲷微卫星标记47个,从中精选出13个标记,组成"分子判别标记组";模拟识别结果表明,此标记组具有充足的分子判别能力,并确定LOD临界值为7.19;使用该临界值为判别标准,在240尾回捕个体中共识别出7尾放流个体,放流个体占比为2.92%。本研究可为黑鲷及其它海洋生物增殖放流效果的准确评估提供技术基础。
A parentage assignment method was established for Sparus macrocephlus and applied to accurately identify releasing-recapture individuals of S. macrocephlus. Firstly, a S.macrocephlus broodstock population was isolated to oviposit, the fertilized eggs were hatched. A microsatellite marker group for molecular identification was obtained using "high-throughput sequencing method". The broodstock population were genotyped using the marker group. The genotyping data was inputted to Cervus, a parentage analysis software. A parentage assignment simulation was conducted to evaluate the assignment ability of the marker group and determine the critical LOD value. Subsequently, the larvae were cultured to achieve the release standards and released in Daya Bay. After six months the recapture action was carried out. Finally, the marker group and critical LOD value were used to identify the releasing-recapture individuals. As a result,forty-seven polymorphic microsatellite markers were obtained. Thirteen markers were selected to compose the microsatellite marker group for molecular identification.
A parentage assignment method was established for Sparus macrocephlus and applied to accurately identify releasing-recapture individuals of S. macrocephlus. Firstly, a S.macrocephlus broodstock population was isolated to oviposit, the fertilized eggs were hatched. A microsatellite marker group for molecular identification was obtained using "high-throughput sequencing method". The broodstock population were genotyped using the marker group. The genotyping data was inputted to Cervus, a parentage analysis software. A parentage assignment simulation was conducted to evaluate the assignment ability of the marker group and determine the critical LOD value. Subsequently, the larvae were cultured to achieve the release standards and released in Daya Bay. After six months the recapture action was carried out. Finally, the marker group and critical LOD value were used to identify the releasing-recapture individuals. As a result,forty-seven polymorphic microsatellite markers were obtained. Thirteen markers were selected to compose the microsatellite marker group for molecular identification.
引文