嫁接对干旱胁迫下烤烟生理特性的影响
详细信息 查看官网全文
- 英文论文题名：Effects of grafting on physiological characteristics of flue-cured tobacco under drought stress
- 论文作者：向金友 ; 谢冰 ; 杨懿德 ; 饶在生 ; 杨洋 ; 易蔓 ; 周先国
- 英文论文作者：Xiang Jinyou ; Xie Bing ; Yang Yide ; Rao Zaisheng ; Yang Yang ; Yi Man ; Zhou Xianguo ; Yibin Tobacco Company of Sichuan Province
- 年：2016
- 作者机构：四川省烟草公司宜宾市公司;
- 论文关键词：嫁接 ; 干旱胁迫 ; 生理特性 ; 酶活性 ; 烤烟 ; 抗旱性
- 英文论文关键词：grafting ; drought stress ; physiological characteristic ; enzyme activity ; flue-cured tobacco ; droughtresistance
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：8
- 文件大小：1590k
- 原文格式：D
摘要
以KRK26为接穗,K326和安烟2号为砧木,通过盆栽试验研究了不同程度干旱水平下嫁接对烟株生理特性的影响,探讨了嫁接对烤烟抗旱性的影响。结果表明,在前期干旱条件下,烟株的叶绿素相对含量(SPAD值)降低,脯氨酸含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性以及过氧化物酶(POD)活性升高。随着干旱程度的加剧和生育期的推进,嫁接苗和自根苗的脯氨酸含量持续升高,而SPAD值、SOD活性、CAT活性、POD活性则呈现下降趋势,但降幅不同,嫁接苗降幅较低。干旱胁迫下除SPAD值优势不明显外,KRK26/安烟2号的其它指标均优于KRK26/K326和KRK26。KRK26/安烟2号在水分胁迫时受到的伤害程度要小于KRK26/K326、KRK26,其中KRK26遭受损害最重。研究表明,嫁接烟株的生长特性对土壤干旱的响应存在明显差异。以上结果说明,嫁接技术能提高烟叶重要指标,可用于增强烤烟抗旱性。
Using KRK26 as scion, K326 and Anyan 2 as rootstock, the effects of grafting on physiological characteristics of flue-cured tobacco was studied in pot culture under different water conditions. And then the effects of grafting on drought resistance of flue-cured tobacco was discussed. The results indicated that under drought stress conditions at early period, the relative content of chlorophyll(SPAD readings) decreased, while the content of proline, superoxide dismutase(SOD) activities, catalase(CAT) activities and peroxidase(POD) activities in flue-cured tobacco demonstrated an increasing trend. The content of proline continued to increase in both grafted seedlings and non-grafted seedlings with the aggravation of drought stress when tobacco leaves were turning to mature, whereas, the SPAD readings, SOD activities, CAT activities and POD activities all demonstrated a decreasing trend. The speed of decreasing in grafted seedlings was slower than that in non-grafted seedlings. Compared with KRK26/K326 and KRK26, KRK26/Anyan 2 had greater parameters under drought stress, but the SPAD readings did not have significant difference. All the results showed that less damage was made to KRK26/Anyan 2 than KRK26/K326 and KRK26 under drought stress, and KRK26 suffered the most serious damage. In conclusion, there were obvious differences among grafted tobacco in physiological characteristics, and there response to soil drought. These results indicated that grafting techniques improve the key parameters influe-cured tobacco leaf and can be used toenhance drought resistance.
Using KRK26 as scion, K326 and Anyan 2 as rootstock, the effects of grafting on physiological characteristics of flue-cured tobacco was studied in pot culture under different water conditions. And then the effects of grafting on drought resistance of flue-cured tobacco was discussed. The results indicated that under drought stress conditions at early period, the relative content of chlorophyll(SPAD readings) decreased, while the content of proline, superoxide dismutase(SOD) activities, catalase(CAT) activities and peroxidase(POD) activities in flue-cured tobacco demonstrated an increasing trend. The content of proline continued to increase in both grafted seedlings and non-grafted seedlings with the aggravation of drought stress when tobacco leaves were turning to mature, whereas, the SPAD readings, SOD activities, CAT activities and POD activities all demonstrated a decreasing trend. The speed of decreasing in grafted seedlings was slower than that in non-grafted seedlings. Compared with KRK26/K326 and KRK26, KRK26/Anyan 2 had greater parameters under drought stress, but the SPAD readings did not have significant difference. All the results showed that less damage was made to KRK26/Anyan 2 than KRK26/K326 and KRK26 under drought stress, and KRK26 suffered the most serious damage. In conclusion, there were obvious differences among grafted tobacco in physiological characteristics, and there response to soil drought. These results indicated that grafting techniques improve the key parameters influe-cured tobacco leaf and can be used toenhance drought resistance.
引文
[1]刘国顺.烟草栽培学[M].北京:中国农业出版社,2005.
[2]Bihmidine S,Cao M X,Kang M,et al.Expression of Chlorovirus MT325 aquaglyceroporin(aqpv1)in tobacco and its role in mitigating drought stress[J].Planta,2014,240(1):209-221.
[3]Liu X,Liu S,Wu J,et al.Overexpression of Arachishypogaea NAC3 in tobacco enhances dehydration and drought tolerance by increasing superoxide scavenging[J].Plant physiology and biochemistry,2013,70:354-359.
[4]Zhai S,Gao Q,Liu X,et al.Overexpression of a Zea mays phospholipase C1 gene enhances drought tolerance in tobacco in part by maintaining stability in the membrane lipid composition[J].Plant Cell:Tissue and Organ Culture,2013,115(2):253-262.
[5]梅芳,杨铁钊,刘剑君,等.嫁接对烤烟氮代谢关键酶活性及叶绿素含量的影响[J].西北农业学报,2012,21(5):132-135.
[6]韩锦峰,汪耀富,岳翠凌,等.干旱胁迫下烤烟光合特性和氮代谢研究[J].华北农学报,1994,9(2):39-45.
[7]Xie H,Yang D H,Yao H,et al.i TRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco(Nicotianatabacum)in response to drought stress[J].Biochemical and biophysical research communications,2016,469(3):768-775.
[8]王鹏翔.干旱胁迫对烤烟形态结构、主要生理指标及化学成分的影响[D].贵阳:贵州大学,2008.
[9]Alameda D,Anten N P R,Villar R.Soil compaction effects on growth and root traits of tobacco depend on light,water regime and mechanical stress[J].Soil and Tillage Research,2012,120:121-129.
[10]Seraji R A N,Navaey H N,Ali S O,et al.Evaluating resistance to drought stress in flue-cured tobacco varieties via stress susceptibility indexes in dry farming condition[J].International Journal of Farming and Allied Sciences,2014,3:373-376.
[11]Ahmad P,Hameed A,Abd-Allah E F,et al.Biochemical and Molecular Approaches for Drought Tolerance in Plants[M]//Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment.Springer New York,2014:1-29.
[12]Drake M P,Vann M C,Fisher L R.Influence of nitrogen application rate on the yield,quality,and chemical components of flue-cured tobacco,part II:Application method[J].Tobacco Science,2015:26-34.
[13]邓桂秀,田飞,赵继平,等.成熟期干旱胁迫对烤烟产量和品质的影响[J].贵州农业科学,2012,40(9):93-96.
[14]Sánchez-Rodríguez E,Romero L,Ruiz J M.Role of grafting in resistance to water stress in tomato plants:ammonia production and assimilation[J].Journal of plant growth regulation,2013,32(4):831-842..
[15]曾建敏,姚恒,李天福,等.烤烟叶片叶绿素含量的测定及其与SPAD值的关系[J].分子植物育种,2009,7(1):56-62.
[16]Liang X,Zhang L,Natarajan S K,et al.Proline mechanisms of stress survival[J].Antioxidants&redox signaling,2013,19(9):998-1011.
[17]Ravet K,Pilon M.Copper and iron homeostasis in plants:the challenges of oxidative stress[J].Antioxidants&redox signaling,2013,19(9):919-932.
[18]Park S,Lee D E,Jang H,et al.Melatonin-rich transgenic rice plants exhibit resistance to herbicide-induced oxidative stress[J].Journal of Pineal Research,2013,54(3):258-263..
[19]Francoz E,Ranocha P,Nguyen-Kim H,et al.Roles of cell wall peroxidases in plant development[J].Phytochemistry,2015,112:15-21.
[20]王新建,何威,杨淑红,等.干旱胁迫下4种楸树嫁接苗叶绿素含量的变化[J].经济林研究,2008,26(1):20-24.
[21]CvikrováM,GemperlováL,MartincováO,et al.Effect of drought and combined drought and heat stress on polyamine metabolism in proline-over-producing tobacco plants[J].Plant physiology and biochemistry,2013,73:7-15.
[22]周冀衡,王彦亭,余佳斌,等.干旱条件下氮肥形态对烤烟叶片内源保护酶活性的影响[J].中国烟草科学,2001,2:5-8.
[23]蒋选利,李振歧,康振生.过氧化物酶与植物抗病性研究进展[J].西北农林科技大学学报:自然科学版,2001,29(6):124-129.
[2]Bihmidine S,Cao M X,Kang M,et al.Expression of Chlorovirus MT325 aquaglyceroporin(aqpv1)in tobacco and its role in mitigating drought stress[J].Planta,2014,240(1):209-221.
[3]Liu X,Liu S,Wu J,et al.Overexpression of Arachishypogaea NAC3 in tobacco enhances dehydration and drought tolerance by increasing superoxide scavenging[J].Plant physiology and biochemistry,2013,70:354-359.
[4]Zhai S,Gao Q,Liu X,et al.Overexpression of a Zea mays phospholipase C1 gene enhances drought tolerance in tobacco in part by maintaining stability in the membrane lipid composition[J].Plant Cell:Tissue and Organ Culture,2013,115(2):253-262.
[5]梅芳,杨铁钊,刘剑君,等.嫁接对烤烟氮代谢关键酶活性及叶绿素含量的影响[J].西北农业学报,2012,21(5):132-135.
[6]韩锦峰,汪耀富,岳翠凌,等.干旱胁迫下烤烟光合特性和氮代谢研究[J].华北农学报,1994,9(2):39-45.
[7]Xie H,Yang D H,Yao H,et al.i TRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco(Nicotianatabacum)in response to drought stress[J].Biochemical and biophysical research communications,2016,469(3):768-775.
[8]王鹏翔.干旱胁迫对烤烟形态结构、主要生理指标及化学成分的影响[D].贵阳:贵州大学,2008.
[9]Alameda D,Anten N P R,Villar R.Soil compaction effects on growth and root traits of tobacco depend on light,water regime and mechanical stress[J].Soil and Tillage Research,2012,120:121-129.
[10]Seraji R A N,Navaey H N,Ali S O,et al.Evaluating resistance to drought stress in flue-cured tobacco varieties via stress susceptibility indexes in dry farming condition[J].International Journal of Farming and Allied Sciences,2014,3:373-376.
[11]Ahmad P,Hameed A,Abd-Allah E F,et al.Biochemical and Molecular Approaches for Drought Tolerance in Plants[M]//Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment.Springer New York,2014:1-29.
[12]Drake M P,Vann M C,Fisher L R.Influence of nitrogen application rate on the yield,quality,and chemical components of flue-cured tobacco,part II:Application method[J].Tobacco Science,2015:26-34.
[13]邓桂秀,田飞,赵继平,等.成熟期干旱胁迫对烤烟产量和品质的影响[J].贵州农业科学,2012,40(9):93-96.
[14]Sánchez-Rodríguez E,Romero L,Ruiz J M.Role of grafting in resistance to water stress in tomato plants:ammonia production and assimilation[J].Journal of plant growth regulation,2013,32(4):831-842..
[15]曾建敏,姚恒,李天福,等.烤烟叶片叶绿素含量的测定及其与SPAD值的关系[J].分子植物育种,2009,7(1):56-62.
[16]Liang X,Zhang L,Natarajan S K,et al.Proline mechanisms of stress survival[J].Antioxidants&redox signaling,2013,19(9):998-1011.
[17]Ravet K,Pilon M.Copper and iron homeostasis in plants:the challenges of oxidative stress[J].Antioxidants&redox signaling,2013,19(9):919-932.
[18]Park S,Lee D E,Jang H,et al.Melatonin-rich transgenic rice plants exhibit resistance to herbicide-induced oxidative stress[J].Journal of Pineal Research,2013,54(3):258-263..
[19]Francoz E,Ranocha P,Nguyen-Kim H,et al.Roles of cell wall peroxidases in plant development[J].Phytochemistry,2015,112:15-21.
[20]王新建,何威,杨淑红,等.干旱胁迫下4种楸树嫁接苗叶绿素含量的变化[J].经济林研究,2008,26(1):20-24.
[21]CvikrováM,GemperlováL,MartincováO,et al.Effect of drought and combined drought and heat stress on polyamine metabolism in proline-over-producing tobacco plants[J].Plant physiology and biochemistry,2013,73:7-15.
[22]周冀衡,王彦亭,余佳斌,等.干旱条件下氮肥形态对烤烟叶片内源保护酶活性的影响[J].中国烟草科学,2001,2:5-8.
[23]蒋选利,李振歧,康振生.过氧化物酶与植物抗病性研究进展[J].西北农林科技大学学报:自然科学版,2001,29(6):124-129.