HPLC-DAD法检测三种兽用中药注射液中非法添加喹乙醇和乙酰甲喹的方法研究
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- 英文论文题名：Study on The Method of HPLC-DAD for The Determination of Olaquindox and Mequindox Illegally Added in Three Kinds of Veterinary Drug Injection of Traditional Chinese Medicine
- 论文作者：李树纲 ; 丁兴莉 ; 张蕾 ; 巨福星 ; 王立娟
- 英文论文作者：LI Shu-gang ; DING Xing-li ; ZHANG-Lei ; JU Fu-xing ; WANG Li-juan ; Gansu Institute of Veterinary Drug and Feed Inspection ; General Hospital Pharmacy Department of Lanzhou Military Area Command
- 年：2016
- 作者机构：甘肃省兽药饲料监察所;兰州军区总医院药剂科;
- 论文关键词：喹乙醇 ; 乙酰甲喹 ; 非法添加物 ; 兽用中药注射液 ; HPLC-DAD
- 英文论文关键词：olaquindox ; mequindox ; illegal additives ; veterinary drug injection of traditional chinese medicine ; HPLC-DAD
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S853.76
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：9
- 文件大小：1602k
- 原文格式：O
- 会议级别：全国
摘要
建立了HPLC-DAD法测定三种兽用中药注射液中非法添加喹乙醇和乙酰甲喹的方法。以十八烷基键合硅胶为填充剂,乙腈—水(13:87)为流动相,用二极管阵列检测器进行全波长(200~400nm)扫描,检测波长为258nm,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。结果表明,该色谱条件下喹乙醇、乙酰甲喹与其他物质峰分离良好。喹乙醇、乙酰甲喹在0.5~200μg/mL浓度范围内线性良好,回收率为93.2%~109.9%,RSD<2.8%,喹乙醇、乙酰甲喹的检测限分别为60μ咖L和100μg/mL。本方法简便快捷、准确度和灵敏度高,能满足非法添加物检测的有关法规和要求。
The method of HPLC-DAD for the determination of olaquindox and mequindox illegally added in three kinds of veterinary drμg injection of traditional Chinese medicine was established.It was tested with C18 column.The mobile phase was acetonitrile-water(13:87).It was used diode array detector for wavelength scanning(200~400nm).The detection wavelength was 258 nm.The Peak purity test and spectral similarity test with standards sample was adopted as assistant control method to identify illegally added drugs.The results showed that under the chromatographic condition,it was separated well for olaquindox,mequindox and other materials.The linearity of olaquindox and mequindox was good in the linear range of 0.5~200μg/mL.The recovery of olaquindox and mequindox is 93.2%~109.9%,RSD≤2.8%.The detection limits of olaquindox and mequindoxt were 60μg/mL and 100μg/mL.This method is simple,high accuracy and sensitivity,and can meet the relevant regulations and requirements of illegal additives detection.
The method of HPLC-DAD for the determination of olaquindox and mequindox illegally added in three kinds of veterinary drμg injection of traditional Chinese medicine was established.It was tested with C18 column.The mobile phase was acetonitrile-water(13:87).It was used diode array detector for wavelength scanning(200~400nm).The detection wavelength was 258 nm.The Peak purity test and spectral similarity test with standards sample was adopted as assistant control method to identify illegally added drugs.The results showed that under the chromatographic condition,it was separated well for olaquindox,mequindox and other materials.The linearity of olaquindox and mequindox was good in the linear range of 0.5~200μg/mL.The recovery of olaquindox and mequindox is 93.2%~109.9%,RSD≤2.8%.The detection limits of olaquindox and mequindoxt were 60μg/mL and 100μg/mL.This method is simple,high accuracy and sensitivity,and can meet the relevant regulations and requirements of illegal additives detection.
引文
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[9]中华人民共和国农业部公告第1868号,氟喹诺酮类制剂中非法添加乙酰甲喹、喹乙醇等化学药物检查方法[S].2012.
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[12]吴萍,张明,许彬.白头翁散、苍术香连散中非法添加喹乙醇、乙酰甲喹检查法-高效液相色谱法检测限的测定[J].现代畜牧兽医,2011,6:51-53.
[2]中国兽药典委员会.中华人民共和国兽药典兽药使用指南化学药品卷二○一○年版[S].北京:中国农业出版社,2011.3.
[3]吴宁鹏,王丽景,李慧素,等.五中兽药中非法添加喹乙醇和乙酰甲喹的HPLC-PDA检测方法的建立[J].中国兽医杂志,2014,48(12):43-49.
[4]吴双民,李剑.畜禽喹乙醇中毒[J].中国兽医杂志,1999,25(5):50-52.
[5]耿毅,汪开毓.动物喹乙醇中毒研究进展[J].畜牧业,2000,5:31-32.
[6]苏志强.浅谈肉鸡乙酰甲喹中毒[J].今日畜牧兽医,2007,(6):42.
[7]刘迎春,高怀涛.乙酰甲喹的合理应用[J].养禽与禽病防治,2009,(3):42-43.
[8]杜子明,杨林洲,李淑娟,等.鸡乙酰甲喹中毒的诊断和防制[J].畜牧兽医杂志,2008,27(1):118-120.
[9]中华人民共和国农业部公告第1868号,氟喹诺酮类制剂中非法添加乙酰甲喹、喹乙醇等化学药物检查方法[S].2012.
[10]农医发[2009]17号,附件3:中兽药散剂中非法添加喹乙醇、乙酰甲喹检查方法[S].
[11]周艳飞,李洽,王亚芳.HPLC法测定乙酰甲喹和喹乙醇的方法研究[J].饲料研究,2013,(4):67-69.
[12]吴萍,张明,许彬.白头翁散、苍术香连散中非法添加喹乙醇、乙酰甲喹检查法-高效液相色谱法检测限的测定[J].现代畜牧兽医,2011,6:51-53.