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臭灵丹精油化学成分及其活性研究
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摘要
研究臭灵丹(Laggera pterodonta(DC.)Benth.)精油组成以及其中木香成分与抗菌活性:利用气味活性追踪分离的方法分析精油化学组成,并从臭灵丹精油具有木香气味的部位分离并鉴定了12个化合物,通过气味评价,推断该植物木香部位的气味主要是由于这些分离到的倍半萜化合物的贡献。利用GC-MS和GC-FID气相色谱联用技术分析云南4个不同地区(昆明,丽江,普洱,新平)臭灵丹精油化学组成。同时,对这些不同产地的臭灵丹挥发油也进行了抗菌活性测试。研究结果表明,采自这四个地区植物样品提取得到的挥发油化学组成以及抗菌活性均不相同。说明不同的化学组成可能是导致4种样品抗菌敏感程度不同的原因。
The woody notes constituents and antimicrobial activities of laggera pterodonta were investigated by means of analyzing their chemical composition of essential oils:By focusing on woody note fractions of the essential oil,one new bisabolane-type sesquiterpenoid,10-hydroxy-2,7(14),ll-bisabolatriene,together with eleven known compounds were isolated by using olfactory-guided fractionation.On the basis of preliminary odor assessment,it suggests that the odor of the woody note fractions of the essential oil was assumed to be due to these isolated sesquiterpenoids.The essential oils obtained by hydrodistillation from leaves of L.pterodonta collected in 4 different locations(Kunming,Lijiang,Puer,Xinping) in Yunna,China were analyzed by GC-MS and GC-FID.The chemical compositions of the oils in four locations were different from each other.Antimicrobial effects of essential oils from four places were also tested.These two parts of experiments suggested that environmental deviations might have big influence on chemical composition variations of the essential oils of L.pterodonta and difference of chemical compositions may lead to dissimilar sensitivity against microbes among these four samples.
引文
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