仙人掌果多糖的结构、理化性质与溶液构象特征
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- 英文论文题名：Structure,physicochemical properties and solution conformation characterization of polysaccharide from the fruit of Opuntia dillenii haw
- 论文作者：韩雨露 ; 汪彬慧 ; 章宝 ; 陈寒青
- 英文论文作者：Han Yulu ; Wang Binhui ; Zhang Bao ; Chen Hanqing ; School of Food Science and Engineering ; Hefei University of Technology
- 年：2016
- 作者机构：合肥工业大学食品科学与工程学院;
- 论文关键词：仙人掌果 ; 多糖 ; 结构 ; 理化性质 ; 溶液构象
- 英文论文关键词：opuntia dillenii haw.fruit ; polysaccharide ; structure ; physicochemical property ; solution conformation
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.23
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：102k
- 原文格式：O
- 会议级别：全国
摘要
本文采用水提醇沉法,从仙人掌果果肉中提取得到粗多糖,粗多糖经过脱色脱蛋白后,再经过DEAE-52纤维素和Sephacryl S-400凝胶柱层析进一步分离纯化,获得一个均一的酸性多糖组分(OFPP)。通过采用GC、HPGPC、FT-IR、SEM、Raman光谱、TG-DSC、XRD、AFM、圆二色谱、刚果红实验等技术手段研究了OFPP的初级结构、理化性质及溶液构象特征。结果表明,该多糖组分分子量为1.98×10~3KDa,糖醛酸含量为34.29%,且主要由鼠李糖(40.8%)、半乳糖(34.5%)、阿拉伯糖(14.4%)、葡萄糖(5.9%)、岩藻糖(1.8%)、木糖(1.6%)和甘露糖(1%)等组成。研究还表明,OFPP表面形态为宽松的片状,呈现半结晶状,并且具有良好的热稳定性。OFPP在水溶液中缺乏三股螺旋构象,可能呈现球状构象。AFM进一步证实了OFPP在水溶液中存在分子球状构象,并且单一分子直径约为26m,高度约为5.9nm。
An acidic polysaccharide fraction named as OFPP was isolated and purified from Opuntia dillenii haw fruits by hot water extraction,alcohol precipitation,decoloration,deproteinization,DEAE cellulose 52 and Sephacryl S-400 column chromatography.The primary structure,physicochemical properties and solution conformation characterization of OFPP were investigated by GC,HPGPC,FT-IR,SEM,Raman,TG-DSC,XRD,AFM,circular dichroism spectroscopy and congo red method.The results revealed that OFPP was a homogeneous heteropolysaccharide with an average molecular weight of 1.98 x 103 KDa.Uronic acid content of OFPP was 34.29%,and it consisted of rhamnose(40.8%),galactose(34.5%),arabinose(14.4%),glucose(5.9%),fucose(1.8%),xylose(1.6%),and mannose(1%).OFPP exhibited good thermal stability and loose flaky morphology with semi-crystalline structure.Moreover,the results indicated that OFPP lacked triple-helix conformation and exhibited sphere-like conformation in the solution.In addition,the AFM images further confirmed that OFPP exhibited a spherical conformation in aqueous solution with the molecular diameter of 26 nm and the height of 5.9 nm.
An acidic polysaccharide fraction named as OFPP was isolated and purified from Opuntia dillenii haw fruits by hot water extraction,alcohol precipitation,decoloration,deproteinization,DEAE cellulose 52 and Sephacryl S-400 column chromatography.The primary structure,physicochemical properties and solution conformation characterization of OFPP were investigated by GC,HPGPC,FT-IR,SEM,Raman,TG-DSC,XRD,AFM,circular dichroism spectroscopy and congo red method.The results revealed that OFPP was a homogeneous heteropolysaccharide with an average molecular weight of 1.98 x 103 KDa.Uronic acid content of OFPP was 34.29%,and it consisted of rhamnose(40.8%),galactose(34.5%),arabinose(14.4%),glucose(5.9%),fucose(1.8%),xylose(1.6%),and mannose(1%).OFPP exhibited good thermal stability and loose flaky morphology with semi-crystalline structure.Moreover,the results indicated that OFPP lacked triple-helix conformation and exhibited sphere-like conformation in the solution.In addition,the AFM images further confirmed that OFPP exhibited a spherical conformation in aqueous solution with the molecular diameter of 26 nm and the height of 5.9 nm.
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