尼古丁与胃蛋白酶相互作用机理初步研究
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- 英文论文题名：Preliminaryinvestigation on the interaction mechanism betweennicotineand pepsin
- 论文作者：韩敬美 ; 廖晓祥 ; 袁大林 ; 雷萍 ; 郑绪东 ; 樊瑛 ; 尚善斋 ; 汤建国 ; 李晖 ; 陈永宽
- 英文论文作者：Han Jing-mei ; Liao Xiao-xiang ; Yuan Da-lin ; Lei Ping ; Zheng Xu-dong ; Fan Ying ; Shan Shang-zhai ; Li Zhi-qiang ; Tang Jian-guo ; Li Hui ; Chen Yong-kuan ; Non-traditional Tobacco Products Institute ; Technology Center of Yunnan Industrial of China Tobacco Industry CO. ; Ltd ; Si Chuan university ; School of Chemical Engineering
- 年：2016
- 作者机构：云南中烟技术中心新型烟草制品研究所;四川大学化学工程学院;
- 论文关键词：尼古丁 ; 胃蛋白酶 ; 光谱学 ; 分子对接 ; 作用机理
- 英文论文关键词：nicotine ; pepsin ; spectroscopy ; molecular-docking ; interaction mechanism
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：中文
- 分类号：R96
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：14
- 文件大小：1956k
- 原文格式：D
摘要
为了探究口含烟尼古丁(NIC)在人体胃部的吸收特性,在模拟胃生理条件下(pH=2.0),利用多种光谱学方法和分子模拟对接技术,初步研究了NIC与胃蛋白酶(Pepsin)相互作用机理。光谱学与分子对接结果表明:1)NIC与Pepsin之间为静态荧光猝灭机制;2)NIC与Pepsin之间通过氢键和范德华力自发的发生相互作用;3)NIC与Pepsin之间存在着一个高亲和力的结合位点;4)加入NIC导致Pepsin构象发生变化,同时对Pepsin活性呈明显的增强效应。以上研究结果对口含烟产品开发、质量控制和安全评价具有重要指导意义。
In order to explore the absorption characteristics of snus nicotine(NIC)in the human stomach, the interaction mechanism between NIC and pepsin was preliminary studied using a variety of spectroscopic methods and molecular docking simulation technologyunder simulated gastric physiological conditions(p H = 2.0). The research of spectroscopy and molecular dockingindicated that 1) the quenching mechanism of NIC on pepsin was static quenching; 2) the interactionbetween NIC and pepsin wasbased onhydrogen bonding and van der waalsforces; 3) a single high-affinity binding site existed between NIC and pepsin;4)NIC had effect on the structure of pepsin and at the same time NIC enhanced the activity of pepsin when it was inhaled into the stomach.The above research results have important guiding significance for the development, quality control and safety evaluation of snus products.
In order to explore the absorption characteristics of snus nicotine(NIC)in the human stomach, the interaction mechanism between NIC and pepsin was preliminary studied using a variety of spectroscopic methods and molecular docking simulation technologyunder simulated gastric physiological conditions(p H = 2.0). The research of spectroscopy and molecular dockingindicated that 1) the quenching mechanism of NIC on pepsin was static quenching; 2) the interactionbetween NIC and pepsin wasbased onhydrogen bonding and van der waalsforces; 3) a single high-affinity binding site existed between NIC and pepsin;4)NIC had effect on the structure of pepsin and at the same time NIC enhanced the activity of pepsin when it was inhaled into the stomach.The above research results have important guiding significance for the development, quality control and safety evaluation of snus products.
引文
[1]薛泽春,程新胜,杨丽文,等.尼古丁猝灭牛血清蛋白荧光机制的研究[J].上海交通大学学报:农业科学版,2006,24(1):44-47.
[2]俞银姣.吸烟的危害与尼古丁替代疗法[J].药物不良反应杂志,2008,10(5):346-351.
[3]邓林园,方晓义.尼古丁依赖的神经生物学机制[J].心理科学进展,2005,13(4):534-543.
[4]陈韵,孔祥荣,沈星灿,等.尼古丁与BSA相互作用的光谱研究[J].光谱学与光谱分析,2005,25(10):1652-1657.
[5]李步海,刘宇飞,孙小梅,等.巴氯芬与胃蛋白酶相互作用的光谱研究[J].中南民族大学学报:自然科学版,2011,30(1):21-24.
[6]Xiaoxiang Liao,Jianguo Tang,Jiawei He,Dalin Yuan,Bing Liang,Qiang Chen,and Hui Li.A New Perspective on Probing the Interaction of Nicotine with Human Serum Albumin[J].Current Analytical Chemistry,2015,DOI:10.2174/1573411012666151203202103.
[7]Xiaoxiang Liao,Dalin Yuan,Jianguo Tang,Hongqin Yang,Bing Liang,Qiang Cheng,and Hui Li.Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin:NMR,Fluorescence,and FTIR Approaches[J].Journal of Spectroscopy,2016,2016(4):1-9.
[8]梁会丽.黄酮类化合物与胃蛋白酶的相互作用及其对酶活性影响的研究[D].郑州大学,2014.
[9]G.Kortüm,H.Wilski,The mechanism of quenching of fluorescence,Transactions of the Faraday Society,1955,51:1620-1623.
[10]E.Gokoglu,F.Kipcak,Z.Seferoglu,Studies on the interactions of 3,6-diaminoacridine derivatives with human serum albumin by fluorescence spectroscopy,Luminescence:the journal of biological and chemical luminescence,2014,29:872-877.
[11]李步海,刘宇飞,孙小梅,等.巴氯芬与胃蛋白酶相互作用的光谱研究[J].中南民族大学学报(自然科学版),2011,01:21-24.
[12]H.J.Zeng,J.You,H.L.Liang,T.Qi,R.Yang,L.B.Qu,Investigation on the binding interaction between silybin and pepsin by spectral and molecular docking,International journal of biological macromolecules,2014,67:105-111.
[13]Q.Wang,S.R.Zhang,X.Ji,Investigation of interaction of antibacterial drug sulfamethoxazole with human serum albumin by molecular modeling and multi-spectroscopic method,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2014,124:84-90.
[14]M.Zolfagharzadeh,M.Pirouzi,A.Asoodeh,M.R.Saberi,J.Chamani,A comparison investigation of DNP-binding effects to HSA and HTF by spectroscopic and molecular modeling techniques,Journal of biomolecular structure&dynamics,2014,32:1936-1952.
[15]A.Samanta,S.Jana,D.Ray,N.Guchhait,Modulated photophysics of a cationic DNA-staining dye inside protein bovine serum albumin:study of binding interaction and structural changes of protein,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2014,121:23-34.
[16]季婉茹,程可,葛凯,等.苯唑西林钠与胃蛋白酶相互作用的光谱研究[J].淮北师范大学学报(自然科学版),2014,03:24-29.
[17]M.Ying,F.Huang,H.Ye,H.Xu,L.Shen,T.Huan,S.Huang,J.Xie,S.Tian,Z.Hu,Z.He,J.Lu,K.Zhou,Study on interaction between curcumin and pepsin by spectroscopic and docking methods,International journal of biological macromolecules,2015,79:201-208.
[18]Y.Wang,Y.Mo,L.Zhou,Synthesis of Cd Se quantum dots using selenium dioxide as selenium source and its interaction with pepsin,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2011,79:1311-1315.
[19]肖敏,邵从英,葛宝宝,等.头孢曲松钠与胃蛋白酶相互作用的光谱研究[J].淮北师范大学学报(自然科学版),2014,04:27-31.
[20]H.J.Zeng,H.L.Liang,J.You,L.B.Qu,Study on the binding of chlorogenic acid to pepsin by spectral and molecular docking,Luminescence:the journal of biological and chemical luminescence,2014,29:715-721.
[21]J.Yan,Q.Wang,Q.Pan,Z.Rao,Y.Su,H.Li,Assessment of the interaction between fraxinellone and bovine serum albumin by optical spectroscopy and molecular modeling methods,Journal of Luminescence,2013,137:180-185.
[22]D.Roy,S.Dutta,S.S.Maity,S.Ghosh,A.S.Roy,K.S.Ghosh,S.Dasgupta,Spectroscopic and docking studies of the binding of two stereoisomeric antioxidant catechins to serum albumins,Journal of Luminescence,2012,132:1364-1375.
[23]D.M.Byler,H.Susi,Examination of the secondary structure of proteins by deconvolved FTIR spectra,Biopolymers,1986,25:469-487.
[24]M.R.Housaindokht,Z.Rouhbakhsh Zaeri,Z.Sharifi,Magnetic field effect on the binding of HSA with amlodipine and propranolol,Journal of Luminescence,2013,137:64-69.
[25]N.Shahabadi,A.Khorshidi,N.H.Moghadam,Study on the interaction of the epilepsy drug,zonisamide with human serum albumin(HSA)by spectroscopic and molecular docking techniques,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2013,114:627-632.
[26]Wu G,Robertson DH,Brooks CL,Vieth M.Detailed analysis of grid‐based molecular docking:A case study of CDOCKER—A CHARMm‐based MD docking algorithm.J Comput Chem 2003,24(13):1549-1562.
[27].
[28]L.Guo,X.Ma,J.Yan,K.Xu,Q.Wang,H.Li,Interaction Behavior Between Niclosamide and Pepsin Determined by Spectroscopic and Docking Methods,Journal of fluorescence,2015,25:1681-1693.
[2]俞银姣.吸烟的危害与尼古丁替代疗法[J].药物不良反应杂志,2008,10(5):346-351.
[3]邓林园,方晓义.尼古丁依赖的神经生物学机制[J].心理科学进展,2005,13(4):534-543.
[4]陈韵,孔祥荣,沈星灿,等.尼古丁与BSA相互作用的光谱研究[J].光谱学与光谱分析,2005,25(10):1652-1657.
[5]李步海,刘宇飞,孙小梅,等.巴氯芬与胃蛋白酶相互作用的光谱研究[J].中南民族大学学报:自然科学版,2011,30(1):21-24.
[6]Xiaoxiang Liao,Jianguo Tang,Jiawei He,Dalin Yuan,Bing Liang,Qiang Chen,and Hui Li.A New Perspective on Probing the Interaction of Nicotine with Human Serum Albumin[J].Current Analytical Chemistry,2015,DOI:10.2174/1573411012666151203202103.
[7]Xiaoxiang Liao,Dalin Yuan,Jianguo Tang,Hongqin Yang,Bing Liang,Qiang Cheng,and Hui Li.Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin:NMR,Fluorescence,and FTIR Approaches[J].Journal of Spectroscopy,2016,2016(4):1-9.
[8]梁会丽.黄酮类化合物与胃蛋白酶的相互作用及其对酶活性影响的研究[D].郑州大学,2014.
[9]G.Kortüm,H.Wilski,The mechanism of quenching of fluorescence,Transactions of the Faraday Society,1955,51:1620-1623.
[10]E.Gokoglu,F.Kipcak,Z.Seferoglu,Studies on the interactions of 3,6-diaminoacridine derivatives with human serum albumin by fluorescence spectroscopy,Luminescence:the journal of biological and chemical luminescence,2014,29:872-877.
[11]李步海,刘宇飞,孙小梅,等.巴氯芬与胃蛋白酶相互作用的光谱研究[J].中南民族大学学报(自然科学版),2011,01:21-24.
[12]H.J.Zeng,J.You,H.L.Liang,T.Qi,R.Yang,L.B.Qu,Investigation on the binding interaction between silybin and pepsin by spectral and molecular docking,International journal of biological macromolecules,2014,67:105-111.
[13]Q.Wang,S.R.Zhang,X.Ji,Investigation of interaction of antibacterial drug sulfamethoxazole with human serum albumin by molecular modeling and multi-spectroscopic method,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2014,124:84-90.
[14]M.Zolfagharzadeh,M.Pirouzi,A.Asoodeh,M.R.Saberi,J.Chamani,A comparison investigation of DNP-binding effects to HSA and HTF by spectroscopic and molecular modeling techniques,Journal of biomolecular structure&dynamics,2014,32:1936-1952.
[15]A.Samanta,S.Jana,D.Ray,N.Guchhait,Modulated photophysics of a cationic DNA-staining dye inside protein bovine serum albumin:study of binding interaction and structural changes of protein,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2014,121:23-34.
[16]季婉茹,程可,葛凯,等.苯唑西林钠与胃蛋白酶相互作用的光谱研究[J].淮北师范大学学报(自然科学版),2014,03:24-29.
[17]M.Ying,F.Huang,H.Ye,H.Xu,L.Shen,T.Huan,S.Huang,J.Xie,S.Tian,Z.Hu,Z.He,J.Lu,K.Zhou,Study on interaction between curcumin and pepsin by spectroscopic and docking methods,International journal of biological macromolecules,2015,79:201-208.
[18]Y.Wang,Y.Mo,L.Zhou,Synthesis of Cd Se quantum dots using selenium dioxide as selenium source and its interaction with pepsin,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2011,79:1311-1315.
[19]肖敏,邵从英,葛宝宝,等.头孢曲松钠与胃蛋白酶相互作用的光谱研究[J].淮北师范大学学报(自然科学版),2014,04:27-31.
[20]H.J.Zeng,H.L.Liang,J.You,L.B.Qu,Study on the binding of chlorogenic acid to pepsin by spectral and molecular docking,Luminescence:the journal of biological and chemical luminescence,2014,29:715-721.
[21]J.Yan,Q.Wang,Q.Pan,Z.Rao,Y.Su,H.Li,Assessment of the interaction between fraxinellone and bovine serum albumin by optical spectroscopy and molecular modeling methods,Journal of Luminescence,2013,137:180-185.
[22]D.Roy,S.Dutta,S.S.Maity,S.Ghosh,A.S.Roy,K.S.Ghosh,S.Dasgupta,Spectroscopic and docking studies of the binding of two stereoisomeric antioxidant catechins to serum albumins,Journal of Luminescence,2012,132:1364-1375.
[23]D.M.Byler,H.Susi,Examination of the secondary structure of proteins by deconvolved FTIR spectra,Biopolymers,1986,25:469-487.
[24]M.R.Housaindokht,Z.Rouhbakhsh Zaeri,Z.Sharifi,Magnetic field effect on the binding of HSA with amlodipine and propranolol,Journal of Luminescence,2013,137:64-69.
[25]N.Shahabadi,A.Khorshidi,N.H.Moghadam,Study on the interaction of the epilepsy drug,zonisamide with human serum albumin(HSA)by spectroscopic and molecular docking techniques,Spectrochimica acta.Part A,Molecular and biomolecular spectroscopy,2013,114:627-632.
[26]Wu G,Robertson DH,Brooks CL,Vieth M.Detailed analysis of grid‐based molecular docking:A case study of CDOCKER—A CHARMm‐based MD docking algorithm.J Comput Chem 2003,24(13):1549-1562.
[27]
[28]L.Guo,X.Ma,J.Yan,K.Xu,Q.Wang,H.Li,Interaction Behavior Between Niclosamide and Pepsin Determined by Spectroscopic and Docking Methods,Journal of fluorescence,2015,25:1681-1693.
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