碱性成纤维细胞生长因子抑制过氧化氢诱导的骨髓间充质干细胞的凋亡

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• 英文论文题名：Basic fibroblast growth factor protects bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis
• 论文作者：高小青 ; 范光碧 ; 邓莉 ; 郭侃 ; 杜杰
• 英文论文作者：GAO Xiao-qing ; FAN Guang-bi ; DENG Li ; GUO Kan ; DU Jie ; Department of Anatomy and Neurobiology ; Southwest Medical University
• 年：2017
• 作者机构：西南医科大学解剖学教研室及神经生物学研究室;
• 论文关键词：碱性成纤维细胞生长因子 ; 骨髓间充质干细胞 ; 过氧化氢 ; 凋亡
• 英文论文关键词：basic fibroblast growth factor ; bone marrow mesenchymal stem cells ; hydrogen peroxide ; oxidative stress ; apoptosis
• 会议召开时间：2017-08-07
• 会议录名称：中国解剖学会第六届全国解剖学技术学术会议论文汇编
• 语种：中文
• 分类号：R329.2
• 学会代码：ZGJP
• 会议名称：中国解剖学会第六届全国解剖学技术学术会议
• 会议地点：中国江西九江
• 主办单位：中国解剖学会科技开发和咨询工作委员会
• 学会名称：中国解剖学会
• 页数：4
• 文件大小：2099k
• 原文格式：D
• 会议级别：全国

摘要

目的观察碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)对过氧化氢(H2O2)诱导的骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)凋亡的保护作用,并探讨其机制。方法用b FGF干预H2O2处理的BMSCs,MTT法检测b FGF处理的BMSCs活性,LDH试剂盒检测细胞培养液乳酸脱氢酶(LDH)漏出量,TUNEL法检测细胞凋亡率,ELISA法检测细胞上清液中IL-6和TNF-α的表达及细胞中Bcl-2/Bax比值。结果 b FGF能显著改善细胞活性(P<0.05),降低LDH漏出量及凋亡率(P<0.05),减少细胞上清液中IL-6和TNF-α的表达(P<0.05),上调细胞的Bcl-2/Bax比值(P<0.05)。结论 b FGF可能通过减少细胞IL-6和TNF-α的表达以及上调Bcl-2/Bax比值,从而增强BMSCs的抗H2O2损伤能力。
Objective This study was designed to investigate protective effect and potential mechanisms of basic fibroblast growth factor(b FGF) against hydrogen peroxide(H2O2)-induced apoptosis of bone marrow mesenchymal stem cells(BMSCs).Methods b FGF treated BMSCs exposed to H2O2.MTT assay was used to detect the cell viability,LDH assay was performed to text lactate dehydrogenase leakage,DAPI staining and TUNEL assay was used to check apoptosis,and ELISA assay was served to detect the expression of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) and Bcl-2/Bax ratio.Results b FGF treatment significantly improved viability of BMSCs(P<0.05),attenuated LDH leakage and apoptosis(P<0.05),decreased the levels of TNF-α and IL-6 and increased expession ratio of Bcl-2/Bax in H2O2-induced BMSCs(P<0.05).Conclusion b FGF increases the anti-apoptotic ability of BMSCs by decreasing the levels of TNF-α and IL-6 and increasing expession ratio of Bcl-2/Bax.

引文

[1]Liu Y,Yi XC,Guo G,et al.Basic fibroblast growth factor increases the transplantation-mediated therapeutic effect of bone mesenchymal stem cells following traumatic brain injury[J].Molecular Medicine Reports,2014,9(1):333-339.
    [2]O'Driscoll C,Wallace D,Cotter TG.b FGF promotes photoreceptor cell survival in vitro by PKA-mediated inactivation of glycogen synthase kinase 3beta and CREB-dependent Bcl-2 up-regulation[J].Journal of Neurochemistry,2007,103(3):860-870.
    [3]Wang Z,Wang Y,Ye J,et al.b FGF attenuates endoplasmic reticulum stress and mitochondrial injury on myocardial ischaemia/reperfusion via activation of PI3K/Akt/ERK1/2 pathway[J].Journal of Cellular and Molecular Medicine,2015,19(3):595-607.
    [4]Coombes E,Jiang J,Chu XP,et al.Pathophysiologically relevant levels of hydrogen peroxide induce glutamate-independent neurodegeneration that involves activation of transient receptor potential melastatin 7channels[J].Antioxidants&Redox Signaling,2011,14(10):1815-1827.
    [5]Shen JN,Xu LX,Shan L,et al.Neuroprotection of(+)-2-(1-Hydroxyl-4-Oxocyclohexyl)Ethyl Caffeate Against Hydrogen Peroxide and Lipopolysaccharide Induced Injury via Modulating Arachidonic Acid Network and p38-MAPK Signaling[J].Curr Alzheimer Res,2015,12(9):892-902.
    [6]Ma F,Xiao Z,Chen B,et al.Accelerating probliferation of neural stem/progenitor cells in collagen sponges immobilized with engineered basic fibroblast growth factor for nervous system tissue engineering[J].Biomacromolecules,2014,15(3):1062-1068.
    [7]Luo JC,Lin HY,Lu CL,et al.Dexamethasone inhibits basic fibroblast growth factor-stimulated gastric epithelial cell probliferation[J].Biochemical Pharmacology,2008,76(7):841-849.
    [8]Colenci R,da Silva Assun??o LR,Mogami Bomfim SR,et al.Bone marrow mesenchymal stem cells stimulated by b FGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro[J].Archives of Oral Biology,2014,59(3):268-276.
    [9]Wang JJ,Liu YL,Sun YC,et al.Basic Fibroblast Growth Factor Stimulates the probliferation of Bone Marrow Mesenchymal Stem Cells in Giant Panda(Ailuropoda melanoleuca)[J].PLo S One,2015,10(9):e0137712.
    [10]Peluso JJ.Basic fibroblast growth factor(b FGF)regulation of the plasma membrane calcium ATPase(PMCA)as part of an anti-apoptotic mechanism of action[J].Biochemical Pharmacology,2003,66(8):1363-1369.
    [11]Park J,Kang W,Ryu SW,et al.Hepatitis C virus infection enhances TNFα-induced cell death via suppression of NF-κB[J].Hepatology,2012,56(3):831-840.
    [12]Zhang L,Tao L,Shi T,Zhang F,et al.Paeonol inhibits B16F10melanoma metastasis In vitro and In Vivo via disrupting proinflammatory cytokines-mediated NF-κB and STAT3 pathways[J].IUBMB Life,2015,67(10):778-788.
    [13]Di Liddo R,Bertalot T,Schuster A,et al.Anti-inflammatory activity of Wnt signaling in enteric nervous system:in vitro preliminary evidences in rat primary cultures[J].Journal of Neuroinflammation,2015,12:23-41.
    [14]Hu HW,Li XK,Zheng,RY,et al.b FGF expression mediated by a hypoxia-regulated adenoviral vector protects PC12 cell death induced by serum deprivation[J].Biochemical and Biophysical Research,2009,390(1):115-120.
    [15]Zeng B,Chen H,Zhu C,et al.Effects of combined mesenchymal stem cells and heme oxygenase-1 therapy on cardiac performance.Europe Journal[J]Cardiothoracic Surgery,2008,34(4):850-856.
    [16]Nishida S,Nagamine H,Tanaka Y,et al.Protective effect of basic fibroblast growth factor against myocyte death and arrhythmias in acute myocardial infarction in rats[J].Circulation Journal,2003,67(4):334-339.