不同烟草对Cd吸收的敏感性分布(SSD)及烟草中Cd的结合形态
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- 英文论文题名：Species Sensitivity Distributions of Cd-phytotoxicity to Tobacco Cultivars and Binding Forms of Cd in Tobacco Roots
- 论文作者：雷丽萍 ; 刘彬 ; 陈世宝 ; 李宁 ; 郑涵
- 英文论文作者：LEI Li-ping ; LIU Bin ; CHEN Shi-bao ; LI Ning ; ZHENG Han ; Yunnan Academy of Tobacco Sciences ; Key Laboratory of Plant Nutrition and Fertilizer ; Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning ; Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：云南省烟草农业科学研究院;农业部植物营养与施肥重点实验室/中国农科院农业资源与农业区划研究所;
- 论文关键词：烟草 ; Cd ; 物种敏感性分布 ; 转运系数 ; 结合形态
- 英文论文关键词：Tobacco cultivars ; cadmium ; species sensitivity distributions(SSD) ; translocation factors ; binding forms
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：X53;X173
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：8
- 文件大小：1085k
- 原文格式：D
摘要
采用水培试验,结合逻辑斯蒂克模型(Log-logistic)、Burr-III概率函数模型,研究了16种不同烟草对Cd胁迫毒性的剂量-效应关系、物种敏感性分布差异,在此基础上,利用重金属形态连续分析法对不同Cd敏感性烟草根内Cd的结合形态进行了测定。结果表明,16种不同烟草对Cd胁迫存在显著性(P<0.05)差异,基于根伸长毒性的半抑制浓度(EC50)从18.76~60.77 mg.L~(-1),最大相差223.9%;不同品种烟草对Cd毒性的敏感性分布频次具有明显差异,其中Y85为Cd最敏感性品种,而K346为Cd耐性最强品种。对植株体内Cd形态分析结果表明,难溶性Cd的磷酸盐Cd(PO4)n结合态及蛋白质与果胶结合态(FNa Cl)Cd在耐Cd性品种(K326、K346)中的比例大于其在敏感性Cd的品种(Y85、TN86)中的比例,说明以难溶性结合态将Cd束缚在根细胞中降低Cd在植株体内的迁移、转运可能是烟草对Cd胁迫产生耐性的机制之一。
The dose-response curves and the species sensitivity distributions(SSD) of Cd toxicity to 16 different tobacco cultivars were investigated using hydroponic culture using log logistic models and probability function(Burr-III) model. A further investigation on the binding forms of Cd in the roots of the tobacco characterized with varied Cd-sensitivity was made using sequential extraction methods. The results showed that significant differences(p<0.05) of toxicity thresholds(EC50) of Cd to tobacco cultivars were observed based on the root elongation test, the toxicity thresholds of EC50 ranged from 18.76~60.77 mg.L~(-1), with an maximum variation of 223.9% among the cultivars. The accumulated frequency of species sensitivity distributions(SSD) of Cd varied significantly(p<0.05) among the tested tobacco cultivars, the most Cd-sensitivity cultivar was Y85 and the highest Cd-tolerance was K346. The result based on the analysis of binding forms of Cd indicated that the ratios of insoluble Cd binding with phosphate Cd(PO4)n and pectin bound Cd in Cd-tolerance cultivars(K346 and K326) were greater than that of Cd-sensitivity cultivars of Y85 and TN86, the result implied that the binding of Cd with insoluble forms in root cells to decrease the migration and transportation of Cd in plant may be one of the Cd-tolerance mechanism for tobaccos.
The dose-response curves and the species sensitivity distributions(SSD) of Cd toxicity to 16 different tobacco cultivars were investigated using hydroponic culture using log logistic models and probability function(Burr-III) model. A further investigation on the binding forms of Cd in the roots of the tobacco characterized with varied Cd-sensitivity was made using sequential extraction methods. The results showed that significant differences(p<0.05) of toxicity thresholds(EC50) of Cd to tobacco cultivars were observed based on the root elongation test, the toxicity thresholds of EC50 ranged from 18.76~60.77 mg.L~(-1), with an maximum variation of 223.9% among the cultivars. The accumulated frequency of species sensitivity distributions(SSD) of Cd varied significantly(p<0.05) among the tested tobacco cultivars, the most Cd-sensitivity cultivar was Y85 and the highest Cd-tolerance was K346. The result based on the analysis of binding forms of Cd indicated that the ratios of insoluble Cd binding with phosphate Cd(PO4)n and pectin bound Cd in Cd-tolerance cultivars(K346 and K326) were greater than that of Cd-sensitivity cultivars of Y85 and TN86, the result implied that the binding of Cd with insoluble forms in root cells to decrease the migration and transportation of Cd in plant may be one of the Cd-tolerance mechanism for tobaccos.
引文
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[2]雷丽萍,陈世宝,夏振远,等.烟草对污染土壤中镉胁迫的响应机制及影响因素研究进展[J].中国烟草科学,2011,32(4):87-93.LEI Li-ping,CHEN Shi-bao,XIA Zhen-yuan,et al.Tolerance and accumulation of cadmium by tobacco plants and the influence factors in polluted soils[J].Chinese Tobacco Science,2011,32(4):87-93.
[3]刘义新,陶涌,孟丽华,等.烤烟品种K326和云烟87对镉胁迫的生理响应及抗性差异[J].中国烟草科学,2008,29(4):1-5.LIU Yi-xin,TAO Yong,MENG Li-hua,et al.Physiological response and resistance of K326 and Yunyan87 to cadmium[J].Chinese Tobacco Science,2008,29(4):1-5.
[4]贺远,王树声,刘海伟,等.镉浓度对烤烟幼苗镉含量及生长和生理指标的影响[J].中国烟草科学,2014,35(2):37-42.HE Yuan,WANG Shu-sheng,LIU Hai-wei,et al.Effect of Cd concentration on Cd content and growth and physiological indices of tobacco seedlings[J].Chinese Tobacco Science,2014,35(2):37-42.
[5]殷永超,吉普辉,宋雪英,等.龙葵(Solanum nigrum L.)野外场地规模Cd污染土壤修复试验[J].生态学杂志,2014,33(11):3060-3067.YIN Yong-chao,JI Pu-hui,SONG Xue-ying,et al.Field experiment on phytoremediation of cadmium contaminated soils using Solanum nigrum L[J].Chinese Journal of Ecology,2014,33(11):3060-3067.
[6]魏树和,周启星,王新.超积累植物龙葵及其对镉的富集特征[J].环境科学,2005,26(3):167-171.WEI Shu-he,ZHOU Qi-xing,WANG Xin.Cadmium-hyperaccumulator solanum nigrum L.and its accumulating characteristics[J].Environmental Science,2005,26(3):167-171.
[7]International Organization for Standardization.Soil quality determination of the effects of pollutants on soil flora,Part 1:method for the measurement of inhibition of root growth,ISO 11269-1[S].1993,Geneva,Switzerland.
[8]鲁如坤.2000.土壤农业化学分析方法.北京:中国农业科技出版社.Lu Ru-kun.Analytical methods for soils and agricultural chemistry.China Agricultural Science and Technology Press[M],2000,Beijing.
[9]Zhang W,Lin K F,Zhou J,et al.Cadmium accumulation,subcellular distribution and chemical forms in rice seedling in the presence of sulfur[J].Environmental toxicology and pharmacology,2014,37:348-353.
[10]Wang X,Liu Y G,Zeng G M,et al.Sub-cellular distribution and chemical forms of cadmium in Bechmeria nivea gaud[J].Environ.Exp.Bot,2008,62,389-395.
[11]Haanstra L,Doelman P,Voshaar J H O.The use of sigmoidal dose response curves in soil ecotoxicological research[J].Plant Soil,2005,84(2):293-297.
[12]魏威,梁东丽,陈世宝.土壤中外源锌对不同植物毒性的敏感性分布[J].生态学杂志,2012,31(3):538-543.WEI Wei,LIANG Dong-li,CHEN Shi-bao.Plant species sensitivity distribution to the phytotoxicity of soil exogenous zinc[J].Chinese Journal of Ecology,2012,31(3):538-543.
[13]Iannone M F,Groppa M D,Benavides M P.Cadmium induces different biochemical responses in wild type and catalase-deficient tobacco plants[J],Environmental and Experimental Botany,2015,109:201-211.
[14]Rosén K,Eriksson J,Vinichuk M.Uptake and translocation of 109Cd and stable Cd within tobacco plants[J].Journal of Environmental Radioactivity,2012,113:16-20.
[15]Moulin N L,Zhang M,Gadani F,et al.Critical review of the science and options for reducing cadmium in tobacco and other plants[J].Advances in Agronomy,2004,83,111-180.
[16]王小庆,李句梅,韦东普等,土壤中铜生态阈值的影响因素及其预测模型[J].中国环境科学,2014,34(2):445-451.WANG Xiao-qing,LI Ju-mei,WEI Dong-pu,et al.Major soil factors affecting ecological threshold for copper and the predictable models[J].China Environmental Science,
[17]Fu-Liu Xu,Yi-Long Li,Yin Wang et al.Key issues for the development and application of the species sensitivity distribution(SSD)model for ecological risk assessment[J].Ecological Indicators,2015,54:227-237.