耐酸性乳酸乳球菌的高通量筛选及其酸胁迫响应机制解析
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- 英文论文题名：High-throughput screening of acid tolerance Lactococcus lactis and the analysis of acid stress response mechanism
- 论文作者：朱政明 ; 张娟 ; 堵国成 ; 陈坚
- 英文论文作者：Zhu Zhengming ; Zhang Juan ; Du Guocheng ; Chen Jian ; School of Biotechnology ; Jiangnan University ; Key Laboratory of Industrial Biotechnology ; Ministry of Education ; School of Biotechnology ; Jiangnan University ; National Engineering Laboratory for Cereal Fermentation Technology ; Jiangnan University
- 年：2016
- 作者机构：江南大学生物工程学院;江南大学工业生物技术教育部重点实验室;江南大学粮食发酵工艺与技术国家工程实验室;
- 论文关键词：Lactococcus lactis ; 诱变 ; 高通量筛选 ; 酸胁迫 ; 生理响应机制解析
- 英文论文关键词：lactococcus lactis ; mutagenesis ; high-throughput screening ; acid stress ; stress response mechanism
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：102k
- 原文格式：O
- 会议级别：全国
摘要
本研究旨在提升Lactococcus lactis NZ9000的酸胁迫耐受性,进而对突变菌株的酸胁迫响应机制进行解析。通过化学和紫外诱变并结合高通量筛选技术最终分别筛选出一株酸耐受性菌株命名为L.lactis WH101和L.lactisWH103。考察酸胁迫条件下的菌株生长性能发现,在pH 4.5胁迫环境中,突变菌株的OD_(600)值分别达到0.527和0.431,相比较选育前提高了4.5倍和3.3倍。进一步的菌株存活率实验结果表明pH 4.0胁迫培养3h后,突变菌株的存活率分别是原始菌株的22.4和1.9倍。生理学特性分析发现,在酸胁迫环境下,突变菌株细胞内含有较高的胞内ATP和NH_(4+)含量,维持了较高的胞内pH,从而提高突变菌株酸胁迫抗性。研究结果不仅为食品工业中耐酸菌株的筛选提供一种有效的方法同时为提升本菌株的工业应用提供了一种新的策略。
This study aimed to improve the acid tolerance of Lactococcus lactis NZ9000 and analysis the acid stress response mechanism of the parent strain and mutant strain.In this study,chemical(DES) and ultraviolet(UV) mutagenesis combined with high-throughput screening technology were conducted to screen acid tolerance strains which were named as L.lactis WH101 and L.lactis WH103.The growth performance of stains in acid stress were investigated,results indicated that the OD_(600) of L.lactis WH101 and L.lactis WH103 reached 0.527 and 0.431 which improved 4.5 and 3.3 times compared with parent strain under acid stress(pH 4.5).Further experiments showed that the survival rate of mutant strains were 22.4 and1.9times higher than parent strain respectively under acid stress(pH 4.0 for 3h).Physiological data showed that the cells exhibited higher intracellular NH_(4+) and ATP concentration and maintained high intracellular pH to protect themselves from acid stress.Results showed here not only provided an effective way to screen acid tolerance strains for the food industry,but offered a new strategy to enhance the industrial utility of this strain.
This study aimed to improve the acid tolerance of Lactococcus lactis NZ9000 and analysis the acid stress response mechanism of the parent strain and mutant strain.In this study,chemical(DES) and ultraviolet(UV) mutagenesis combined with high-throughput screening technology were conducted to screen acid tolerance strains which were named as L.lactis WH101 and L.lactis WH103.The growth performance of stains in acid stress were investigated,results indicated that the OD_(600) of L.lactis WH101 and L.lactis WH103 reached 0.527 and 0.431 which improved 4.5 and 3.3 times compared with parent strain under acid stress(pH 4.5).Further experiments showed that the survival rate of mutant strains were 22.4 and1.9times higher than parent strain respectively under acid stress(pH 4.0 for 3h).Physiological data showed that the cells exhibited higher intracellular NH_(4+) and ATP concentration and maintained high intracellular pH to protect themselves from acid stress.Results showed here not only provided an effective way to screen acid tolerance strains for the food industry,but offered a new strategy to enhance the industrial utility of this strain.
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