Inhibitors of Eicosanoid Biosynthesis Influencing the Transcripts Level of sHSP21.4 Gene Induced by Pathogen Infections, in Antheraea pernyi
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- 英文论文题名：Inhibitors of Eicosanoid Biosynthesis Influencing the Transcripts Level of sHSP21.4 Gene Induced by Pathogen Infections, in Antheraea pernyi
- 论文作者：Congfen Zhang ; Lishang Dai ; Lei Wang ; Cen Qian ; Guoqing Wei ; Jun Li ; Baojian Zhu ; Chaoliang Liu
- 英文论文作者：Congfen Zhang ; Lishang Dai ; Lei Wang ; Cen Qian ; Guoqing Wei ; Jun Li ; Baojian Zhu ; Chaoliang Liu ; College of Life Science ; Anhui Agricultural University
- 年：2016
- 作者机构：College of Life Science, Anhui Agricultural University;
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S885.1
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：498k
- 原文格式：D
- 会议级别：全国
摘要
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of s HSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a s Hsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 k Da protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus(NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with ds RNA result in the decrease at the expression levels of several immune response-related genes(defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of s HSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a s Hsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 k Da protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus(NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with ds RNA result in the decrease at the expression levels of several immune response-related genes(defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.
Small heat shock proteins(s HSPs) can regulate protein folding and protect cells from stress. To investigate the role of s HSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a s Hsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 k Da protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus(NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with ds RNA result in the decrease at the expression levels of several immune response-related genes(defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.
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