Molecular regulation of bract suppression during panicle development in rice

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• 英文论文题名：Molecular regulation of bract suppression during panicle development in rice
• 论文作者：Lei Wang ; Shengyuan Sun ; Caiguo Xu ; Xianghua Li ; Jinghua Xiao ; Qifa Zhang
• 英文论文作者：Lei Wang ; Shengyuan Sun ; Caiguo Xu ; Xianghua Li ; Jinghua Xiao ; Qifa Zhang ; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) ; Huazhong Agricultural University
• 年：2016
• 作者机构：National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University;
• 英文论文关键词：transcription factor ; reproductive transition ; inflorescence architecture
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：S511
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：842k
• 原文格式：D
• 会议级别：全国

摘要

The reproductive transition in rice is characterized by a number of dramatic changes. Two of the most striking features of these changes are the suppression of bract,and the change of the lateral organ identity from tiller branching into panicle branching. Both of these features indicate suppression of the vegetative programs for panicle development. However,the underlying mechanisms are not well known. We identified a mutant panicle with ectopic leaf(pel) that failed to suppress bract outgrowth. PEL had two paralogs in rice known as PEL-like 1 and 2(PEL1,2). All three PELs were expressed in the bract primordium revealed by in situ hybridization,and PEL proteins formed homodimers and heterodimers in vitro and in vivo. We used CRISPR/Cas9 to generate single gene and triple-gene mutants. Much more bracts were formed in the triple mutant pel pel1 pel2 than any single gene mutant. Moreover,the panicle branches were also completely supplanted by the vegetative shoots in the pel pel1 pel2 plants. Therefore,PELs redundantly terminated the vegetative programs after reproductive transition. Y2 H was used to screen the interacting proteins of PELs,and one protein PEL Interacted Protein 1(PIP1) was identified. Pull-down,Bi FC and LCI further substantiated the physical interactions between PIP1 and PELs in vitro and in planta. Moreover,PIP1 was co-expressed with the PELs based on the global transcriptomic analysis,suggesting that PELs might regulate PIP1 or vice versa. The results of Y1 H,EMSA and Ch IP-q PCR demonstrated that PELs directly regulated PIP1 in rice. Genetic analysis showed that PIP1 also repressed bract outgrowth. All of these genes encoded transcription factors,suggesting that they should have vital roles in reprogramming the transcriptomes following the reproductive transition. Several genomic approaches will be used to address this question,and the related works are underway. According to these results,a complex network of genes required for terminating the vegetative programs after reproductive transition is emerging.
The reproductive transition in rice is characterized by a number of dramatic changes. Two of the most striking features of these changes are the suppression of bract,and the change of the lateral organ identity from tiller branching into panicle branching. Both of these features indicate suppression of the vegetative programs for panicle development. However,the underlying mechanisms are not well known. We identified a mutant panicle with ectopic leaf(pel) that failed to suppress bract outgrowth. PEL had two paralogs in rice known as PEL-like 1 and 2(PEL1,2). All three PELs were expressed in the bract primordium revealed by in situ hybridization,and PEL proteins formed homodimers and heterodimers in vitro and in vivo. We used CRISPR/Cas9 to generate single gene and triple-gene mutants. Much more bracts were formed in the triple mutant pel pel1 pel2 than any single gene mutant. Moreover,the panicle branches were also completely supplanted by the vegetative shoots in the pel pel1 pel2 plants. Therefore,PELs redundantly terminated the vegetative programs after reproductive transition. Y2 H was used to screen the interacting proteins of PELs,and one protein PEL Interacted Protein 1(PIP1) was identified. Pull-down,Bi FC and LCI further substantiated the physical interactions between PIP1 and PELs in vitro and in planta. Moreover,PIP1 was co-expressed with the PELs based on the global transcriptomic analysis,suggesting that PELs might regulate PIP1 or vice versa. The results of Y1 H,EMSA and Ch IP-q PCR demonstrated that PELs directly regulated PIP1 in rice. Genetic analysis showed that PIP1 also repressed bract outgrowth. All of these genes encoded transcription factors,suggesting that they should have vital roles in reprogramming the transcriptomes following the reproductive transition. Several genomic approaches will be used to address this question,and the related works are underway. According to these results,a complex network of genes required for terminating the vegetative programs after reproductive transition is emerging.

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