摘要
通过PCR直接测序的方法获得我国现有5种鹿亚科物种线粒体DNA控制区(D-loop)全序列,并结合GenBank检索到我国的2种鹿亚科动物同源序列进行分析。结果显示:我国鹿亚科动物D-loop区序列全长在921~1 072bp之间,序列中存在重复单元,碱基T、A、C、G的平均含量分别为32.1%、30.2%、22.7%及15.0%,各物种间的遗传距离范围在0.064~0.106,处于属间差异,支持将麋鹿和豚鹿并入鹿属的观点。以控制区全序列为基础构建的系统进化树结果表明梅花鹿、马鹿和白唇鹿亲缘较近,它们与水鹿构成一组进化枝,而坡鹿与麋鹿构成一组进化枝,以上两枝最后与豚鹿并在一起。
引文
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