新疆北部地区牛源及人源耐甲氧西林金黄色葡萄球菌的检测及耐药性分析
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- 英文论文题名：Detecion and Drug Resistance Analysis of Methicillin-resistant Staphylococcus aureus Strains Isolated from Bovine Mastitis and Human in North Xinjiang
- 论文作者：李善春 ; 江航 ; 袁贝 ; 马鹏睿 ; 易鸳鸯 ; 苏艳
- 英文论文作者：Li Shanchun ; Jiang Hang ; Yuan Bei ; Ma Pengrui ; Yi Yuanyang ; Su Yan ; College of Veterinary Medicine ; Xinjiang Agriculture University
- 年：2016
- 作者机构：新疆农业大学动物医学学院;
- 论文关键词：新疆北部地区 ; 牛乳房炎 ; MRSA ; mecA基因 ; nuc基因 ; 耐药性
- 英文论文关键词：Bovine mastitis ; MRSA ; Antimicrobial resistance analysis ; MecA gene ; Nuc gene
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：中文
- 分类号：R440;S858.23
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：8
- 文件大小：645k
- 原文格式：D
- 会议级别：全国
摘要
为了解新疆北部地区奶牛乳房炎及临床健康人群中耐甲氧西林金黄色葡萄球菌(MRSA)感染和耐药现状。对2014-2015年采集的新疆北部地区规模化奶牛场临床型和隐性乳房炎乳样和健康人群的咽拭子,经分离纯化后mec A基因的PCR扩增结果鉴定得到19株牛源及1株人源MRSA。采用K-B纸片扩散法和最小抑菌浓度(MIC)法检测20株MRSA对20种药物的敏感性。19株牛源分离菌株MRSA菌株对头孢呋辛,头孢噻呋,头孢西丁,磺胺嘧啶钠,克林霉素,氨苄西林六种药物耐药率大于80%,20株菌对头孢呋辛耐药率高达100%;分离菌株多重耐药情况严重,其中耐受10种以上药物的菌株占94.8%;人源分离菌株对四环素,氨苄西林,链霉素,磺胺甲恶唑敏感,对16种药物耐药。研究显示新疆北部地区牛源及人源MRSA分离菌株的多重耐药现象较为严重,牛源MRSA的存在及多重耐药将该地区该病的抗菌药物治疗形成挑战。
In order to investigate the current status of the infection and the antimicrobial resistance of methicillin-resistant Staphylococcus aureus(MRSA) isolates from bovine mastitis and health human.19 MRSA strains from bovine and 1 MRSA strain from human were isolated and detection of mec A gene by PCR assay were performed to detect the presence of MRSA.K-B disk diffusion and minimum inhibitory concentration(MIC) value method were used to determine the antimicrobial susceptibility of these MRSA to 20 different antimicrobial agents.Our result indicated that total of 20 MRSA isolate were detected and the result of resistance assay revealed that the resistant rate of ampicillin,cefuroxime,ceftiofur,cefoxitin,sulfadiazine sodium,clindymycin was more than80% and resistant rate of cefuroxime was 100%.This investigation suggested that multi-drug resistance of these isolates was very serious and more than 94.8% MRSA strains resisted to 10-16 types antibiotics.For MRSA strain isolated from human,it sensitive to Tetracycline,ampicillin,streptomycin and sulfafurazole,it also showed multi-drug resistance to 16 types antibiotics.This data indicated that multi-drug antimicrobial resistance of the MRSA isolated from bovine mastitis and health people in North Xinjiang was very serious,the existence of bovine MRSA and its multi-drug resistance will pose a big challenge to the antimicrobial treatment of bovine mastitis and the survey will provide rational basis for cow mastitis treatment.
In order to investigate the current status of the infection and the antimicrobial resistance of methicillin-resistant Staphylococcus aureus(MRSA) isolates from bovine mastitis and health human.19 MRSA strains from bovine and 1 MRSA strain from human were isolated and detection of mec A gene by PCR assay were performed to detect the presence of MRSA.K-B disk diffusion and minimum inhibitory concentration(MIC) value method were used to determine the antimicrobial susceptibility of these MRSA to 20 different antimicrobial agents.Our result indicated that total of 20 MRSA isolate were detected and the result of resistance assay revealed that the resistant rate of ampicillin,cefuroxime,ceftiofur,cefoxitin,sulfadiazine sodium,clindymycin was more than80% and resistant rate of cefuroxime was 100%.This investigation suggested that multi-drug resistance of these isolates was very serious and more than 94.8% MRSA strains resisted to 10-16 types antibiotics.For MRSA strain isolated from human,it sensitive to Tetracycline,ampicillin,streptomycin and sulfafurazole,it also showed multi-drug resistance to 16 types antibiotics.This data indicated that multi-drug antimicrobial resistance of the MRSA isolated from bovine mastitis and health people in North Xinjiang was very serious,the existence of bovine MRSA and its multi-drug resistance will pose a big challenge to the antimicrobial treatment of bovine mastitis and the survey will provide rational basis for cow mastitis treatment.
引文
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[4]Weese J S,Duijkeren E V.Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine.Veterinary Microbiology,2010,140:418-429.
[5]欧阳范献.MRSA的SCCmec分子分型及其耐药性研究[D].海南海口:华南热带农业大学,2007.
[6]Devriese L A,Van Dammme L R,Fameree L.Methicillin-(cloxacillin)-resistant Staphylococcus aureus strains isolated from bovine mastitis case.Zentralbl.Veterinarmed,1972,8(19):598-605.
[7]Ferber D.Livestock feed ban preserves druds′power.Science,2002,295(5552):27-28.
[8]Juhasz-Kaszanyitzkyé,Janosi S,Somogyi P,Daná,Bloois L G,Duijkeren E V,Wagenaar J A.MRSAtransmission between Cows and humans.Emerging Infectious Disease,2007,13(4):630-632.
[9]Lee J H.Methicillin(oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans.Appled Environmental Microbiology,2003,69(11):6489-6494.
[10]Turutoglu H,Huasoksuz H,Ozturk D,Yildirim M,Sagnak S.Methicillin and aminoglycoside resistance in Staphylococcus aureus isolates from bovine mastitis and sequence analysis of their mec A genes.Veterinary Research Communications,2009,33:945-956.
[11]Türkyilmaz S,Tekbiyik S,Oryasin E,Bozdogan B.Molecular epidemiology and antimicrobial resistance mechanisms of meyhicillin-resistant Staphylococcus aureus isolated from bovine milk.Zoonoses and Public Health,2010,57:197-203.
[12]Fe?le A,Scott C,Kadlec K,Ehricht R,Monecke S,Schwarz S.Characterization of methicillin-resistant Staphylococcus aureus ST398 from case of bovine mastitis.Journal of Antimicrob Chemother,2010,65:619-625.
[13]Wei Y Y,Wang X,Hao F,Yu Y.The classification and identification of pathogenic methicillin-resistant Staphylococcus aureus isolated from bovine mastitis.China Dairy,2011,109(1):50-52.(in Chinese)
[14]王登峰,王治才,杨学云,等.我国牛源金黄色葡萄球菌耐药现状研究及药敏试验方法探讨[J].中国动物传染病学报,2011,19(1):31-39.
[15]Moon J S,Lee A R,Kang H M,Lee E S,Kim M N,Paik Y H,Park Y H,Joo Y S,Koo H C.Phenotypic and genetic antibiogram of methicillin-resistant Staphylococci isolated from bovine mastitis in Korea.Journal of Dairy Sciences,2007,90:1176-1185.
[16]Galdiero E,Liguori G,Isanto M D,Damiano N,Sommese L.Distribution of mec A among methicilllin-resistant clinical staphylococcal strains isolated at hospitals in Naples,Italy.European Journal of Epidemiology,2003,18:139-145.
[17]Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing;eighteenth informational supplement,M100-S19[S].Wayne,PA,2009.
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[19]Michael N A,Levy S B.Molecular.mechanisms of antibacterial multidrug resistance.Cell,2007,128(23):1037-1050
[20]Wang G Q,Yang M M,Xing Y,Liu W,Feng R Y.Drug resistance analysis of Staphylococcus aureus from dairy cow mastitis in Ningxia area.Progess in veterinaiy Medicine,2011,32(10):59-62.(in Chinese)
[21]苏洋,蒲万霞,陈智华,邓海平.牛源金黄色葡萄球菌的耐药性及耐甲氧西林金黄色葡萄球菌的检测,中国农业科学.2012,45(17):3602-3607.
[22]杜锐,韩文瑜,雷连成,等.动物源性金黄色葡萄球菌耐药性的检测[J].中国兽医科技,2005,35(3):230-232.
[23]王登峰,李建军,段新华,杨学云,吴建勇,玛利亚木,王治才.我国牛源金黄色葡萄球菌耐药现状及药敏检测方法探讨,中国动物传染病学报2011,19(1):31-38.
[24]章锐锋,万欢英.社区获得性MRSA感染与定植的危险因素分析[J].中华医院感染学杂志,2008,18(7):938-940.
[2]Jürgen S H,B?ebel K,Wilfried W,Michael Z,Axel S,Klaus F.The epidemiology of Staphylococcus aureus infections from subclinical mastitis in dairy cows during a control programme.Veterinary Microbiology,2003,96:91-102.
[3]Talbot B G,Lcasse P.Progress in the development of mastitis vaccines.Livestock Production Science,2005,98(1/2):103-113.
[4]Weese J S,Duijkeren E V.Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine.Veterinary Microbiology,2010,140:418-429.
[5]欧阳范献.MRSA的SCCmec分子分型及其耐药性研究[D].海南海口:华南热带农业大学,2007.
[6]Devriese L A,Van Dammme L R,Fameree L.Methicillin-(cloxacillin)-resistant Staphylococcus aureus strains isolated from bovine mastitis case.Zentralbl.Veterinarmed,1972,8(19):598-605.
[7]Ferber D.Livestock feed ban preserves druds′power.Science,2002,295(5552):27-28.
[8]Juhasz-Kaszanyitzkyé,Janosi S,Somogyi P,Daná,Bloois L G,Duijkeren E V,Wagenaar J A.MRSAtransmission between Cows and humans.Emerging Infectious Disease,2007,13(4):630-632.
[9]Lee J H.Methicillin(oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans.Appled Environmental Microbiology,2003,69(11):6489-6494.
[10]Turutoglu H,Huasoksuz H,Ozturk D,Yildirim M,Sagnak S.Methicillin and aminoglycoside resistance in Staphylococcus aureus isolates from bovine mastitis and sequence analysis of their mec A genes.Veterinary Research Communications,2009,33:945-956.
[11]Türkyilmaz S,Tekbiyik S,Oryasin E,Bozdogan B.Molecular epidemiology and antimicrobial resistance mechanisms of meyhicillin-resistant Staphylococcus aureus isolated from bovine milk.Zoonoses and Public Health,2010,57:197-203.
[12]Fe?le A,Scott C,Kadlec K,Ehricht R,Monecke S,Schwarz S.Characterization of methicillin-resistant Staphylococcus aureus ST398 from case of bovine mastitis.Journal of Antimicrob Chemother,2010,65:619-625.
[13]Wei Y Y,Wang X,Hao F,Yu Y.The classification and identification of pathogenic methicillin-resistant Staphylococcus aureus isolated from bovine mastitis.China Dairy,2011,109(1):50-52.(in Chinese)
[14]王登峰,王治才,杨学云,等.我国牛源金黄色葡萄球菌耐药现状研究及药敏试验方法探讨[J].中国动物传染病学报,2011,19(1):31-39.
[15]Moon J S,Lee A R,Kang H M,Lee E S,Kim M N,Paik Y H,Park Y H,Joo Y S,Koo H C.Phenotypic and genetic antibiogram of methicillin-resistant Staphylococci isolated from bovine mastitis in Korea.Journal of Dairy Sciences,2007,90:1176-1185.
[16]Galdiero E,Liguori G,Isanto M D,Damiano N,Sommese L.Distribution of mec A among methicilllin-resistant clinical staphylococcal strains isolated at hospitals in Naples,Italy.European Journal of Epidemiology,2003,18:139-145.
[17]Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing;eighteenth informational supplement,M100-S19[S].Wayne,PA,2009.
[18]Leonard F C,Markcy B K.Meticillin-resistant Staphylococcus aureus in animals:a rview[J].Vet J,2008175(1):27-36.
[19]Michael N A,Levy S B.Molecular.mechanisms of antibacterial multidrug resistance.Cell,2007,128(23):1037-1050
[20]Wang G Q,Yang M M,Xing Y,Liu W,Feng R Y.Drug resistance analysis of Staphylococcus aureus from dairy cow mastitis in Ningxia area.Progess in veterinaiy Medicine,2011,32(10):59-62.(in Chinese)
[21]苏洋,蒲万霞,陈智华,邓海平.牛源金黄色葡萄球菌的耐药性及耐甲氧西林金黄色葡萄球菌的检测,中国农业科学.2012,45(17):3602-3607.
[22]杜锐,韩文瑜,雷连成,等.动物源性金黄色葡萄球菌耐药性的检测[J].中国兽医科技,2005,35(3):230-232.
[23]王登峰,李建军,段新华,杨学云,吴建勇,玛利亚木,王治才.我国牛源金黄色葡萄球菌耐药现状及药敏检测方法探讨,中国动物传染病学报2011,19(1):31-38.
[24]章锐锋,万欢英.社区获得性MRSA感染与定植的危险因素分析[J].中华医院感染学杂志,2008,18(7):938-940.